Interannual, seasonal, local and body size variations in reproduction of the prawn Penaeus (Marsupenaeus) japonicus (Crustacea: Decapoda: Penaeidae) in the Ariake Sea and Tachibana Bay, Japan

 The reproductive biology of female Penaeus japonicus Bate was investigated in the Ariake Sea and Tachibana Bay (located outside the Ariake Sea), Japan from 1994 to 1996. Interannual, seasonal, individual female body size and spatial influences on the incidence of spawning were examined. The proportion of inseminated females, increased with increasing body size up to 170 mm body length (BL) and decreased thereafter. The minimum size at maturity was similar between years; 130 to 140 mm BL. The minimum size of individuals with developing ovaries or spermatophores differed from that of ripe females by 20 to 25 mm. Spawning occurred in the central part of the Ariake Sea and Tachibana Bay but rarely in the inner part. P. japonicus had a clear reproductive cycle. Spawning started earlier and ended later, occurring from mid-May to mid-October, in the eastern central part of and outside of the Ariake Sea compared with the western central part, where spawning occurred from mid-June to mid-September. Spawning dynamics differed across seasons, body sizes and areas but not across years. The seasonal peak in the proportion of ripe females varied with body size. In small individuals (130 to 169 mm BL), no peak was observed, whereas in large individuals (>170 mm BL) the proportion of ripe individuals peaked in June. The proportion of ripe individuals increased with increasing body size and was high outside the Ariake Sea. Factors causing the variation in spawning dynamics are discussed. Received: 30 November 1998 / Accepted: 12 October 1999     

Predation on meiofaunal and macrofaunal invertebrates by western sandpipers (Calidris mauri): evidence for dual foraging modes

Western sandpiper (Calidris mauri) predation was examined by concurrent experiments and direct observations of foraging behaviour on high intertidal mudflats of the Fraser River estuary, British Columbia. Western sandpipers foraged by either “pecking” on the surface (64% of observational time) or probing into sediment (29%). The first experiment (probe-mark method) consisted of collecting small-volume cores (21.2 cm³) of probed (experimental) and non-probed (control) sediment on the tidal flat, following a 22.5-min feeding period. The second experiment (exclosure method) involved deploying exclosures immediately prior to the feeding period and subsequent collection of cores from inside (control) and outside (experimental) the exclosures. Sediment cores were analysed for both macrofaunal and meiofaunal size fractions. Comparisons between macro- and meiofaunal invertebrate densities in experimental and control sediments revealed significant differences, attributed to shorebird predation, for both experiments. The probe-mark experiment detected the removal of large infaunal polychaetes (∼ 20 mm), while the exclosure experiment showed depletion of epifaunal harpacticoid copepods (0.063–0.5 mm). Predation on macrofaunal cumaceans was detected in both experiments. Invertebrates selected by western sandpipers neither fell within traditional infaunal size classifications (macro- vs. meiofauna; 500 μm delineation) nor corresponded to the highest densities of taxa. Rather, inference from experimental results and observations is that western sandpipers forage in two modes, by: (1) surface gleaning of epibenthic copepods and cumaceans in the macro- and meiofaunal size ranges and (2) selective probing for larger infauna, such as polychaetes. These findings were facilitated by the combination of methodologies employed. Received: 29 December 1999 / Accepted: 11 September 2000     

Grazing rates of organic matter and living fungal biomass of decaying Spartina alterniflora by three species of salt-marsh invertebrates

 The pathway for the flow of salt-marsh grass production into marsh food-webs is still not well defined. We compared the abilities of three marsh macroinvertebrates [salt marsh periwinkles, Littoraria irrorata (Say) (=Littorina irrorata), salt-marsh coffee-bean snails, Melampus bidentatus (Say); and a talitrid amphipod, Uhlorchestia spartinophila Bounsfield and Heard] to access standing-dead leaves of smooth cordgrass (Spartina alterniflora Loisel). The invertebrates were incubated with naturally-decaying leaves, and the rates of removal of organic matter and living fungal biomass (ergosterol) were measured. The impact of invertebrate activity upon fungal growth rates was measured as rates of fungal-membrane synthesis (incorporation of radioacetate into ergosterol). The removal rates of organic leaf biomass per mg individual biomass were highest for amphipods (700 μg mg⁻¹ d⁻¹) and lowest for periwinkles (90 μg mg⁻¹ d⁻¹), but the relatively large biomass of the snails made their removal rates per individual greater than those of amphipods. Net removal of ergosterol by all three invertebrates was >50% for yellow-brown (early-decay) leaf blades. For fully-brown (advanced-decay) blades, >50% removal of ergosterol was found only for periwinkles; exposure to coffee-bean snails and amphipods resulted in a net ergosterol reduction of ≤20%. The lower net reduction of living fungal biomass by coffee-bean snails and amphipods may have been due to fungal-growth stimulation (2.3-fold stimulation in coffee-bean snails and 1.5-fold stimulation in amphipods). Grazing by periwinkles did not stimulate fungal growth, possibly because of its high intensity. Grazing by these three salt-marsh shredders may affect marsh-grass shoot-decay in different ways. Periwinkles may abbreviate the period of fungal production, and incorporate the decaying material relatively quickly into snail biomass and fecal-pellet rain to the sediments. Coffee-bean snails and amphipods may enhance and prolong fungal production, along with the formation of fecal-pellet rain. All three invertebrates fed preferentially on leaf blades rather than leaf sheaths, and feeding rates of gastropods were higher during the night than during the day. Received: 25 November 1998 / Accepted: 4 November 1999     

Reproductive success increases with degree of kinship in cooperative coalitions of female red howler monkeys (Alouatta seniculus)

Evaluation of evolutionary mechanisms proposed to promote cooperative behavior depends on the relative influence of the behavior on the reproductive success of individuals, the reproductive success of the group in which they interact behaviorally, and the degree of gene correlation among cooperators. The genetic relationship within cooperative coalitions of female red howler monkeys was examined for three populations with different densities and growth rates. Patterns of gene correlation change within coalitions is documented using data from the mitochondrial and nuclear genomes, and long-term census monitoring. Differences in fecundity and infant survivorship within and between groups of unrelated (rˉ=0) and related (rˉ≥ 0.25) females are compared. Females that emigrate from their natal groups form coalitions with other migrant females. These coalitions attempt to establish a territory and, once successful in producing offspring, exclude other females from feeding resources. Females in these coalitions had different mtDNA haplotypes and a genetically estimated mean r of 0, supporting demographic data on emigration patterns indicating that these females rarely have the opportunity to form coalitions with kin. Patterns of recruitment and rate of matriline development within social groups supported behavioral data indicating that females actively attempt to promote their own matriline as breeders over that of other females, and that some matrilines are more successful at this than others. Mean r among females was significantly higher in coalitions established as social groups for several generations (rˉ=0.44). In these groups, females all shared the same mtDNA haplotype, and mtDNA haplotype divergence was significantly higher between than within groups. Females in coalitions with kin had significantly higher reproductive success than females in unrelated coalitions in all populations. This difference was not a function of coalition size, number of males, socionomic sex ratio, or primiparity, although anecdotal evidence suggests that allomothering may compensate for inept new mothers in related coalitions more often than in unrelated ones. Differences in territory quality could not be ruled out as a potential causal factor in the saturated populations, but were unlikely in the low-density, growing population. There were substantial differences among long-established coalitions in overall reproductive output in all three populations, and this was significantly correlated with the number of breeding females. Increase in coalition size was a function of both group age and the behavioral tolerance among females. Regardless of the underlying reasons for the patterns observed, reproductive success clearly increases with degree of gene correlation among females within cooperative coalitions, and coalitions that recruit more daughters produce more offspring. The nature of the cooperative relationship among group females directly influences both of these outcomes. This is associated with substantial genetic differentiation among social groups within populations, creating conditions in which genetic tendencies towards cooperative behavior can become tightly associated with group reproductive success. Received: 15 September 1999 / Revised: 27 April 2000 / Accepted: 27 May 2000     

Reproductive success of monogamous and polygynous pied flycatchers (Ficedula hypoleuca)

One of the most interesting aspects of the mating system of pied flycatchers is the regular occurrence of polygyny. Here we present data on the reproductive success of polygynously paired pied flycatcher males compared to monogamous males based on paternity analyses through DNA fingerprinting. Males paired with two females suffered a higher loss in reproductive output per female compared to monogamous males due to (1) a greater proportion of unhatched eggs in their broods, (2) greater nestling mortality and (3) a greater probability of being cuckolded. Nevertheless, the number of fledglings was significantly greater for polygynous males. Based on the number of nestlings that returned for subsequent breeding seasons, however, the reproductive success of monogamous and polygynous males did not differ significantly. These data raise the question as to why males attempt polygyny. Received: 16 August 1999 / Received in revised form: 15 March 2000 / Accepted: 18 March 2000     

Training Captive-Bred or Translocated Animals to Avoid Predators

Abstract: Animal reintroductions and translocations are potentially important interventions to save species from extinction, but most are unsuccessful. Mortality due to predation is a principal cause of failure. Animals that have been isolated from predators, either throughout their lifetime or over evolutionary time, may no longer express appropriate antipredator behavior. For this reason, conservation biologists are beginning to include antipredator training in pre-release preparation procedures. We describe the evolutionary and ontogenetic circumstances under which antipredator behavior may degenerate or be lost, and we use principles from learning theory to predict which elements can be enhanced or recovered by training. The empirical literature demonstrates that training can improve antipredator skills, but the effectiveness of such interventions is influenced by a number of constraints. We predict that it will be easier to teach animals to cope with predators if they have experienced ontogenetic isolation than if they have undergone evolutionary isolation. Similarly, animals should learn more easily if they have been evolutionarily isolated from some rather than all predators. Training to a novel predator may be more successful if a species has effective responses to similar predators. In contrast, it may be difficult to teach proper avoidance behavior, or to introduce specialized predator-specific responses, if appropriate motor patterns are not already present. We conclude that pre-release training has the potential to enhance the expression of preexisting antipredator behavior. Potential training techniques involve classical conditioning procedures in which animals learn that model predators are predictors of aversive events. However, wildlife managers should be aware that problems, such as the emergence of inappropriate responses, may arise during such training.     

Gradient analysis of the chrogenetic structure of lowland and highland scotch pine populations

Gradients of genetic distances (GGDs) between 26 adjacent cenopopulations of Scotch pine (Pinus sylvestris L.) along the transects through the distinct landscape-ecotopic isolation borders were analyzed graphically. The results made it possible to reveal and quantitatively estimate gradients and borders of gene-pool structural patterns in populations of this species in the central part of Northern Eurasia. In lowland areas, the most distinct borders were found between pine populations growing on dry lands and bogs (Pineta sphagnosa) in the forest zone of the Transural region, as well as between the Arakaragaiskii and Amankaragaiskii island pine forests in the steppe zone (Northern Turgai). In highland areas (the Urals and the Carpathians), the greatest GGDs were observed between low-mountain (about 600 m above sea level) and middle-mountain (850–900 m) populations. Analysis of GGDs is a promising gene-geographic method for determining population borders and studying the chorogenetic structure of species.     

Modelling the Distribution and Quality of Sand and Gravel Resources in 3D: a Case Study in the Thames Basin,UK

Three-dimensional (3D) models are often utilised to assess the presence of sand and gravel deposits. Expanding these models to provide a better indication of the suitability of the deposit as aggregate for use in construction would be advantageous. This, however, leads to statistical challenges. To be effective, models must be able to reflect the interdependencies between different criteria (e.g. depth to deposit, thickness of deposit, ratio of mineral to waste, proportion of ‘fines’) as well as the inherent uncertainty introduced because models are derived from a limited set of boreholes in a study region. Using legacy borehole data collected during a systematic survey of sand and gravel deposits in the UK, we have developed a 3D model for a 2400 km² region close to Reading, southern England. In developing the model, we have reassessed the borehole grading data to reflect modern extraction criteria and explored the most suitable statistical modelling technique. The additive log-ratio transform and the linear model of coregionalization have been applied, techniques that have been previously used to map soil texture classes in two dimensions, to assess the quality of sand and gravel deposits in the area. The application of these statistical techniques leads to a model which can be used to generate thousands of plausible realisations of the deposit which fully reflect the extent of model uncertainty. The approach offers potential to improve regional-scale mineral planning by providing an enhanced understanding of sand and gravel deposits and the extent to which they meet current extraction criteria.