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981.
Risky Ayu Kristanti Masahiro Kanbe Tadashi Toyam Yasuhiro Tanak Yueqin Tang Xiaolei Wu Kazuhiro Mori 《环境科学学报(英文版)》2012,24(5):800-807
We investigated the biodegradation of 2-nitrophenol (2-NP), 4-nitrophenol (4-NP), and 2,4-dinitrophenol (2,4-DNP) in the rhizosphere of Spirodela polyrrhiza plants by conducting degradation experiments with three river water samples supplemented with each nitrophenol (NP). We then isolated NP-degrading bacteria both from the S. polyrrhiza roots and from the river water. In the river water samples, removal of the three NP was accelerated in the presence of S. polyrrhiza plants. The three NPs persisted in an autoclaved solution with sterile plants suggests that NP removal was accelerated largely by bacterial NP biodegradation rather than by adsorption and uptake by the plants. We isolated 8 strains of NP-degrading bacteria: 6 strains from the S. polyrrhiza roots and 2 strains from river water without the plants. The 2-NP- and 2,4-DNP-degrading bacteria were isolated only from the S. polyrrhiza roots. The 4-NPdegrading bacteria different from those isolated from the river water samples were also found on S. polyrrhiza roots. The 2-NP- and 4-NP-degrading strains isolated from the roots utilized the corresponding NP (0.5 mmol/L) as the sole carbon and energy source. The 2,4-DNP-degrading strains isolated from the roots showed substantial 2,4-DNP-degrading activity, but the presence of other carbon and energy sources was required for their growth. The isolated NP-degrading bacteria from the roots must have contributed to the accelerated degradation of the three NPs in the rhizosphere of S. polyrrhiza. Our results suggested that rhizoremediation with S. polyrrhiza may be effective for NP-contaminated surface water. 相似文献
982.
983.
报道了久效磷对3种海洋微藻细胞内2种清除活性氧的关键性酶———超氧化物歧化酶和过氧化物酶活性的影响.结果显示:1在久效磷的胁迫下,扁藻和三角褐指藻细胞的超氧化物歧化酶(SOD)活性均表现出下降的总变化趋势,而叉鞭金藻细胞的SOD活性时而上升,时而下降,在整个胁迫过程中呈现出无规律性的变化.2随着久效磷胁迫时间的延长,3种微藻细胞的过氧化物酶活性均逐渐下降,表现出相同的变化规律性.这说明不同的藻种,久效磷对其细胞内酶活性的影响不尽相同.推测超氧化物歧化酶和过氧化物酶(POD)活性的降低是微藻细胞内过量产生活性氧,进而引起藻细胞膜脂过氧化伤害的主要原因之一. 相似文献
984.
恶臭污染源环境影响评价要点问题探讨 总被引:1,自引:0,他引:1
针对城市恶臭发生源的无组织与无组织排放方式及臭气成分复杂且大多属复合臭气成分的特点,重点对恶臭源的监测、组分分析方法以及源强综合评价等关键问题进行了研究和总结。 相似文献
985.
采用微波消解-ICP-AES法测定底泥中的铜、镍等金属元素。通过实验和加标回收率的测定,回收率在88~102%之间。 相似文献
986.
The biosafety of methyl tertiary-butyl ether (MTBE), mainly used as a gasoline additive, has long been a contentious topic. In addition to its routine toxicities, MTBE has been demonstrated to disrupt glucose and lipid metabolism and contribute to the development of type 2 diabetes as well as obesity. As one of the morbidities related to dyslipidemia, atherosclerosis is worthy of being investigated under MTBE exposure. Since foam cells derived from macrophages play pivotal roles during atherosclerosis development, we studied the effects of MTBE on macrophages in vitro and assessed the effect of MTBE on atherosclerosis plaque formation with the ApoE?/? mouse model in vivo for the first time. Our results demonstrated that exposure to MTBE at environmentally relevant concentrations decreased the expression of ABCA1 and ABCG1, which are responsible for macrophage cholesterol efflux, at both mRNA and protein levels in THP-1 macrophages. Consequently, treatment with MTBE inhibited the transport of cholesterol from macrophages to High-density lipoprotein. ApoE?/? mice exposed to MTBE at environmentally relevant concentrations (100, 1000 μg/kg) displayed significant increases in lesion area in the aorta and aortic root compared to vehicle-treated ones. Further analysis indicated that MTBE exposure enhanced the macrophage-specific marker Mac-2 contents within plaques in the aortic root, implying that MTBE could promote macrophage-derived foam cell formation and thus accelerate atherosclerosis plaque formation. We for the first time demonstrated the pro-atherogenic effect of MTBE via eliciting disruption of macrophage cholesterol efflux and accelerating foam cell formation and atherosclerosis plaque development. 相似文献
987.
一株反硝化细菌的分离鉴定及其反硝化特性简 总被引:1,自引:0,他引:1
从处理城镇污水的移动床生物膜反应器中分离获得一株反硝化细菌D3,并进一步研究该菌株的系统发育地位及反硝化特性。采用16S rDNA序列分析对菌株进行初步鉴定,探讨了基质浓度、起始pH和温度对菌株反硝化活性的影响。根据形态学特征、生理生化特性及16S rDNA序列测定分析,初步鉴定菌株属于寡养单胞菌属。该菌能利用硝酸钠或亚硝酸钠进行反硝化作用,最佳电子受体是硝酸盐氮,反硝化速率最大为19.86 mg/(L·h),最适生长pH为7.36,最适生长温度为33.5℃。菌株D3在初始硝态氮浓度为140 mg/L,以乙酸钠为惟一碳源,pH为7.36,温度33.5℃的最优生长条件下,培养10 h进入对数生长期,倍增时间为9.9 h,48 h内硝酸盐还原率达95%。 相似文献
988.
989.
用一种具有多孔结构特征的沸石球作为载体固定化硝化细菌强化吸附氨氮废水。结果表明,沸石球能快速固定化硝化细菌,吸附动力学表明150 min对氨氮吸附容量达到2.816 mg/g,而且还有继续增加的趋势。假二级动力学方程和Elovich模型的精确拟合说明,生物沸石球对氨氮的吸附过程可能是非均相扩散起作用及以化学吸附反应为主的复杂转化过程。扩散拟合结果表明,氨氮在生物沸石球的吸附是以液膜扩散和颗粒内扩散为吸附速率的控制步骤,活性的硝化细菌对氨氮的硝化使氨氮在微孔的吸附得到了强化。 相似文献