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71.
OBJECTIVE: The objective of the study was to determine which vehicle factors are significantly related to pelvic injury in side impact collisions. Identification of relevant parameters could aid in the reduction of these injuries. METHOD: Side impact crashes from the CIREN database were separated into those in which the occupant sustained a pelvic fracture and those in which no pelvic fracture occurred, although all occupants had serious injuries. A multibody MADYMO model was created of a USDOT SINCAP (U.S. Department of Transportation Side Impact New Car Assessment Program) test of a vehicle with a large center console. RESULTS: From a study of 113 side impact crashes in the ciren database, nearside occupants with pelvic fractures (n = 78) had (i) more door intrusion (mean, 37 vs. 32 cm, p = 0.02) than those who had serious injuries, but not pelvic fractures (ii) a greater likelihood that the lower border of the door intruded more than the upper part (40% vs. 18%, p < 0.025); and (iii) a greater likelihood that their vehicle had a center console (47 vs. 17%, p < 0.005). Other parameters such as occupant age, weight, gender, vehicle weight, and struck vehicle speed change were not significantly different. MADYMO modeling showed that with a center console, an initial positive pelvic acceleration occurred at about 30 msec, followed at about 45 msec by a second acceleration peak in the opposite direction. Reducing console stiffness reduced the second acceleration but not the initial peak. Allowing the seat to translate laterally when contacted by the door reduced the initial pelvic acceleration by 50% and eliminated the second acceleration peak. CONCLUSIONS: Redesigning the center console using less stiff materials and allowing some lateral translation of the seat could aid in reducing pelvic injuries in side impact collisions. 相似文献
72.
Metabolism of acetaminophen (paracetamol) in plants—two independent pathways result in the formation of a glutathione and a glucose conjugate 总被引:1,自引:0,他引:1
Christian Huber Bernadett Bartha Rudolf Harpaintner Peter Schröder 《Environmental science and pollution research international》2009,16(2):206-213
Background, aim, and scope Pharmaceuticals and their metabolites are detected in the aquatic environment and our drinking water supplies. The need for
high quality drinking water is one of the most challenging problems of our times, but still only little knowledge exists on
the impact of these compounds on ecosystems, animals, and man. Biological waste water treatment in constructed wetlands is
an effective and low-cost alternative, especially for the treatment of non-industrial, municipal waste water. In this situation,
plants get in contact with pharmaceutical compounds and have to tackle their detoxification. The mechanisms for the detoxification
of xenobiotics in plants are closely related to the mammalian system. An activation reaction (phase I) is followed by a conjugation
(phase II) with hydrophilic molecules like glutathione or glucose. Phase III reactions can be summarized as storage, degradation,
and transport of the xenobiotic conjugate. Until now, there is no information available on the fate of pharmaceuticals in
plants. In this study, we want to investigate the fate and metabolism of N-acetyl-4-aminophenol (paracetamol) in plant tissues using the cell culture of Armoracia rusticana L. as a model system.
Materials and methods A hairy root culture of A. rusticana was treated with acetaminophen in a liquid culture. The formation and identification of metabolites over time were analyzed
using HPLC-DAD and LC–MSn techniques.
Results With LC–MS technique, we were able to detect paracetamol and identify three of its metabolites in root cells of A. rusticana. Six hours after incubation with 1 mM of acetaminophen, the distribution of acetaminophen and related metabolites in the
cells resulted in 18% paracetamol, 64% paracetamol–glucoside, 17% paracetamol glutathione, and 1% of the corresponding cysteine
conjugate.
Discussion The formation of two independently formed metabolites in plant root cells again revealed strong similarities between plant
and mammalian detoxification systems. The detoxification mechanism of glucuronization in mammals is mirrored by glucosidation
of xenobiotics in plants. Furthermore, in both systems, a glutathione conjugate is formed. Due to the existence of P450 enzymes
in plants, the formation of the highly reactive NAPQI intermediate is possible.
Conclusions In this study, we introduce the hairy root cell culture of A. rusticana L. as a suitable model system to study the fate of acetaminophen in plant tissues. Our first results point to the direction
of plants being able to take up and detoxify the model substrate paracetamol. These first findings underline the great potential
of using plants for waste water treatments in constructed wetlands.
Recommendations and perspectives This very first study on the detoxification of a widely used antipyretic agent in plant tissues again shows the flexibility
of plant detoxification systems and their potential in waste water treatment facilities. This study covers only the very first
steps of acetaminophen detoxification in plants; still, there is no data on long-term exposure as well as the possible impact
of pharmaceuticals on the plant health and stress defense. Long-term experiments need to be performed to follow the fate of
acetaminophen in root and leaf cells in a whole plant system, and to evaluate possible usage of plants for the remediation
of acetaminophen from waste water. 相似文献
73.
Bastian Herzog Hilde Lemmer Bettina Huber Harald Horn Elisabeth Müller 《Environmental science and pollution research international》2014,21(4):2795-2804
The intensive use of benzotriazoles as corrosion inhibitors for various applications and their application in dishwasher detergents result in an almost omnipresence of benzotriazole (BTri), 4-methyl- and 5-methyl-benzotriazole (4-TTri and 5-TTri, respectively) in aquatic systems. This study aims on the evaluation of the biodegradation potential of activated sludge communities (ASCs) toward the three benzotriazoles regarding aerobic, anoxic, and anaerobic conditions and different nutrients. ASCs were taken from three wastewater treatment plants with different technologies, namely, a membrane bioreactor (MBR-MH), a conventional activated sludge plant CAS-E (intermittent nitrification/denitrification), and CAS-M (two-stage activated sludge treatment) and used for inoculation of biodegradation setups. All ASCs eliminated up to 30 mg L?1 5-TTri and BTri under aerobic conditions within 2–7 and 21–49 days, respectively, but not under anoxic or anaerobic conditions. 4-TTri was refractory at all conditions tested. Significant differences were observed for BTri biodegradation with non-acclimated ASCs from MBR-MH with 21 days, CAS-E with 41 days, and CAS-M with 49 days. Acclimated ASCs removed BTri within 7 days. Furthermore, different carbon and nitrogen concentrations revealed that nitrogen was implicitly required for biodegradation while carbon showed no such effect. The fastest biodegradation occurred for 5-TTri with no need for acclimatization, followed by BTri. BTri showed sludge-specific biodegradation patterns, but, after sludge acclimation, was removed with the same pattern, regardless of the sludge used. Under anaerobic conditions in the presence of different electron acceptors, none of the three compounds showed biological removal. Thus, presumably, aerobic biodegradation is the major removal mechanism in aquatic systems. 相似文献
74.
Singh RB Huber AH Braddock JN 《Journal of the Air & Waste Management Association (1995)》2007,57(4):420-433
A microscale emission factor model (MicroFacPM) for predicting real-time site-specific motor vehicle particulate matter emissions was presented in the companion paper titled "Development of a Microscale Emission Factor Model for Particulate Matter (MicroFacPM) for Predicting Real-Time Motor Vehicle Emissions". The emission rates discussed are in mass per unit distance with the model providing estimates of fine particulate matter (PM2.5) and coarse particulate matter. This paper complements the companion paper by presenting a sensitivity analysis of the model to input variables and evaluation model outputs using data from limited field studies. The sensitivity analysis has shown that MicroFacPM emission estimates are very sensitive to vehicle fleet composition, speed, and the percentage of high-emitting vehicles. The vehicle fleet composition can affect fleet emission rates from 8 mg/mi to 1215 mg/mi; an increase of 5% in the smoking (high-emitting) current average U.S. light-duty vehicle fleet (compared with 0%) increased PM2.5 emission rates by -272% for 2000; and for the current U.S. fleet, PM2.5 emission rates are reduced by a factor of -0.64 for speeds >50 miles per hour (mph) relative to a speed of 10 mph. MicroFacPM can also be applied to examine the contribution of emission rates per vehicle class, model year, and sources of PM. The model evaluation is presented for the Tuscarora Mountain Tunnel, Pennsylvania Turnpike, PA, and some limited evaluations at two locations: Sepulveda Tunnel, Los Angeles, CA, and Van Nuys Tunnel, Van Nuys, CA. In general, the performance of MicroFacPM has shown very encouraging results. 相似文献
75.
76.
An outdoor model ecosystem was designed for the ecotoxicological evaluation of xenobiotics. Two years were necessary before the artificial pond reached a steady state. During this time the composition of the community and its functions were investigated. We recorded the amount of nutrients and the O(2)/CO(2)-metabolism in the water, the density and diversity of the phytoplankton, the aquatic macrophytes and the fauna, the microbial activity in the sediment, and the environmental impacts on the ecosystem. A short time before the application of sodium pentachlorophenate (Na-PCP) the ecosystem was divided into three identical subunits. One of these was used as an internal control, the others were contaminated with two different concentrations of Na-PCP (0.1 and 0.3 mg litre(-1)). These concentrations were maintained over a period of eight weeks. Ecological changes in the contaminated compartments were investigated during a period of one year. The results were compared with those of single-species tests. Significant variations were observed only in the unit receiving 0.3 mg Na-PCP litre(-1). A short time after starting the experiment, the number of rotifers and cyclopids decreased. Primary producers were not affected. An increase of the chloride levels in the water indicated degradation processes. One year after application of the chemical, the remineralisation of nutrients was disturbed. This resulted in a diminution of the phytoplankton and the aquatic fauna. 相似文献
77.
Peter Schröder Lyudmila Lyubenova Christian Huber 《Environmental science and pollution research international》2009,16(7):795-804
Background, aim and scope
Mixed pollution with trace elements and organic industrial compounds is characteristic for many spill areas and dumping sites. The danger for the environment and human health from such sites is large, and sustainable remediation strategies are urgently needed. Phytoremediation seems to be a cheap and environmentally sound option for the removal of unwanted compounds, and the hyperaccumulation of trace elements and toxic metals is seemingly independent from the metabolism of organic xenobiotics. However, stress reactions, ROS formation and depletion of antioxidants will also cause alterations in xenobiotic detoxification. Here, we investigate the capability of plants to detoxify chlorophenols via glutathione conjugation in a mixed pollution situation. 相似文献78.
A protocol has been proposed for testing seats for whiplash protection, however injury criteria have not yet been chosen. Assuming that whiplash symptoms arise from non-physiological motions of vertebral segments, we determined the ability of proposed criteria to predict peak individual vertebral displacements. Twenty-eight volunteers were subjected to rear impacts while seated in a car seat with head restraint, mounted onto a sled. Accelerometers were used to record head and torso accelerations. The volunteer data was used as a basis for testing post-mortem human specimens (PMHS). The seat was replaced by a platform onto which was mounted each of 11 cervico-thoracic spines. An instrumented headform was mounted to the upper end of the spine. The head restraint, head-to-restraint geometry, sled, and impact pulse remained the same. Head and T1 accelerations were measured and individual vertebral sagittal (XZ) plane rotations and translations were obtained from high speed video. Proposed injury criteria (NIC, Nkm, Nte, Nd) were tested for their ability to predict average, total, and peak intervertebral displacements. PMHS specimens had chest and head X (horizontal) and Z (vertical) linear accelerations similar to volunteers whose heads hit the head restraint. The best predictors were: Nd shear and peak intervertebral posterior translation (r(2) = 0.80), Nd extension and peak extension angle (r(2) = 0.70), and Nd distraction and peak distraction (r(2) = 0.51). Therefore consideration should be given to a displacement based injury criteria such as Nd in assessment of whiplash protection devices. 相似文献
79.
Armando I. Perez Wayne C. Huber James P. Heaney E. E. Pyatt 《Journal of the American Water Resources Association》1972,8(5):900-908
ABSTRACT. A mathematical model to predict water quality in a surface-groundwater system is under development. This project is being sponsored by the Environmental Protection Agency. The ultimate goal of this study is to obtain cause and effect relationships between pollutant sources and the ensuing concentrations at different locations in a basin. Several programs are used to model the various hydrologic processes occurring in nature, namely: rainfall, runoff, flow in surface bodies of water, infiltration, and groundwater flow. At every time step in the simulation, the water quantity computations for the above hydrologic models are performed first. Subsequently, the results of these computations, typically in the form of flow velocities, are used as input to the water quality calculations. The water quality routines involve the modeling of the associated physical, chemical, and biological processes. In this study, emphasis is being placed on pollution in agricultural areas. Accordingly the Lake Apopka basin in Central Florida is being used as the application site. 相似文献
80.
OBJECTIVES: Safety of the airbag supplemental restraint system (airbag) is a well-known concern. Although many lives are saved each year through airbag use, injuries continue to occur, especially to the head. Airbag safety research has focused primarily on severe injuries, while minor and moderate injuries have been largely ignored. METHODS: In this study, 205,977 injury cases from the 1995 to 2001 National Automotive Sampling System (NASS)/ Crashworthiness Data System (CDS) were surveyed to determine the prevalence of AIS 1 and 2 facial and brain loss of consciousness (LOC) injuries and determine if these injuries are a concern. The query was focused on frontal impacts in vehicles equipped with airbags. Only occupants wearing appropriate seatbelts were included in this study so that the airbag would provide occupant protection under optimal conditions. Of the 205,977 injury cases studied, 2.4% met this criterion. RESULTS: From the data gathered, the trends seem to indicate an increase in these specific injuries, both in terms of the total number and the proportion to all injury cases. In 1995, AIS 1 and 2 head injuries accounted for 96.5% of all head injuries caused by airbags. By 2001, the percentage had risen 3.0% to 99.5%. Injuries occurring in vehicles equipped with first-generation versus second generation airbags were compared, and data seem to suggest that there is a higher rate of minor and moderate head injuries when occupants are in second-generation airbag-equipped vehicles, even when appropriate lap and shoulder belts are used. CONCLUSIONS: The short timeframe surveyed prevents drawing meaningful conclusions about statistical significance, but the graphical representations of the data in this study underscore an urgent need for further investigation based on current trends in order to understand the issue of minor and moderate head injury prevention in regard to airbags. 相似文献