Acute effects of 3,4-dichloroaniline on biomarkers and spleen histology of the common goby Pomatoschistus microps

The aromatic amine 3,4-dichloroaniline (DCA) is a model environmental contaminant, precursor for synthesis and degradation product of several herbicides, which is commonly found in European estuarine ecosystems. In this work, the possibility of using biochemical and histological markers to assess sub-lethal effects of DCA in natural populations of Pomatoschistus microps juveniles was investigated. Alterations on the activities of the enzymes acetylcholinesterase (AChE), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) and histological alterations on spleen were investigated after 96 h of exposure to sublethal concentrations of DCA (0.50-1.49 mg/l). At the concentrations tested, DCA had no effect on AChE activity. LDH and GST activities were significant altered in treated animals when compared to control groups. As already described for mammals, DCA induced splenic histological alterations in P. microps, including expansion of red pulp and deposition of hemosiderin granules in a concentration-dependent manner. This suggests that DCA is a xenobiotic of concern in estuaries receiving agricultural effluents.     

Radium-226 in water, sediments, and fish from lakes near the city of Elliot Lake, Ontario, Canada

Ra-226 was measured by alpha-emission spectroscopy in water, sediments, and fish (tissues and gut contents), from five lakes in a watershed containing U mining and milling operations at Elliot Lake, Ontario, and from control lakes in an adjacent non-industrialized watershed. Ra-226 transfer parameters from lake water and sediments to fish tissues, and annual intakes by humans consuming fish, were estimated. Mean dissolved 226Ra levels ranged from approximately 76 mBq litre(-1) in water of the most affected lake, to < 10 mBq litre(-1) in control lakes. Levels in summer were consistently higher than in fall or winter; no consistent variation with depth was noted. Sediment levels ranged from approximately 3000 mBq g(-1) dry wt in one study lake to < 100 mBq g(-1) dry wt of sediment in control lakes. Bone 226Ra concentrations were higher than in muscle. The lake trout (Salvelinus namaycush), a predatory secondary consumer, had bone 226Ra levels (< 20 mBq g(-1) dry wt) that did not show significant site variation. In contrast, bottom feeding whitefish had significantly more 226Ra in bone tissue (to 38 mBq g(-1) dry wt in the lake whitefish, Coregonus clupeaformis, and 76 mBq g(-1) in round whitefish, Prosopium cylindraceum) in study lakes than in controls (< 20 mBq g(-1) dry wt). Ra-226 levels in lake trout muscle were low and showed erratic variation among lakes whereas levels in whitefish muscle did not vary significantly among study and control sites. Lake herring (= cisco, Coregonus artedii), a planktivorous fish taken only from Quirke Lake, had mean 226Ra levels of 18 and 1.4 mBq g(-1) dry wt in bone and muscle, respectively. Gut 226Ra levels, highest in lake trout from McCabe and Quirke Lakes (126 +/- 53, 64 +/- 44 mBq g(-1) dry wt, respectively), and just detectable in McCabe and Elliot Lake whitefish (24 +/- 2, 36 +/- 14 mBq g(-1) dry wt, respectively), were below detection in lake trout and whitefish from other lakes. Concentration ratios (CRs) of 226Ra from water to muscle ranged from 8 to 14 in lake trout, 7 to 14 in whitefish, and 4 to 6 in lake herring. The water to bone CRs varied from 81 to 142, 314 to 548, and 126 to 272 in the same species. CRs were always < 1 between sediments and fish tissues. Annual intake of 226Ra by human consumers of these fish was estimated to provide an effective dose of 0.003 mSv year(-1). This represents a small fraction of both natural background (> 2 mSv year(-1)) and the public dose limit (5 mSv year(-1)).     

Alarming decline of freshwater trigger species in western Mediterranean key biodiversity areas

Theidentification of key biodiversity areas (KBA) was initiated by the International Union for Conservation of Nature in 2004 to overcome taxonomic biases in the selection of important areas for conservation, including freshwater ecosystems. Since then, several KBAs have been identified mainly based on the presence of trigger species (i.e., species that trigger either the vulnerability and or the irreplaceability criterion and thus identify a site as a KBA). However, to our knowledge, many of these KBAs have not been validated. Therefore, classical surveys of the taxa used to identify freshwater KBAs (fishes, molluscs, odonates, and aquatic plants) were conducted in Douro (Iberian Peninsula) and Sebou (Morocco) River basins in the Mediterranean Biodiversity Hotspot. Environmental DNA analyses were undertaken in the Moroccan KBAs. There was a mismatch between the supposed and actual presence of trigger species. None of the trigger species were found in 43% and 50% of all KBAs surveyed in the Douro and Sebou basins, respectively. Shortcomings of freshwater KBA identification relate to flawed or lack of distribution data for trigger species. This situation results from a misleading initial identification of KBAs based on poor (or even inaccurate) ecological information or due to increased human disturbance between initial KBA identification and the present. To improve identification of future freshwater KBAs, we suggest selecting trigger species with a more conservative approach; use of local expert knowledge and digital data (to assess habitat quality, species distribution, and potential threats); consideration of the subcatchment when delineating KBAs boundaries; thoughtful consideration of terrestrial special areas for conservation limits; and periodic field validation.     

Evaluating regulatory issues and emission control equipment for air toxics applications

This article describes regulatory issues and emission control equipment for air toxics applications. A brief history of air quality legislation is provided, illustrating the difficulty government has had in implementing air quality statutes; this has contributed to the complexity of air quality regulations. Evaluation methodologies are described for three different emission control technologies: thermal oxidation, catalytic oxidation, and adsorption. Brief case histories are provided that describe the pitfalls and benefits associated with each technology. The benefits of integrating health risk assessment into an air quality program are also discussed.     

Gulf War agents trigger discrete transcriptional changes in human neuronal cells

Reports on Gulf War illness (GWI) implicated the use of the pesticide permethrin (PER), and the insect repellent N,N-diethyl-m-toluamide (DEET), in its etiology, as well as pyridostigmine bromide (PB), which was given as a prophylactic treatment against nerve agent exposure. Using Affymetrix microarrays the genomic response of human neuronal SHSY-5Y cells to 10 days of exposure to these agents was characterized and profiles of gene modulation unique to each treatment were demonstrated. In particular, a significantly greater impact of PER was observed compared to the other treatments. The Ingenuity Pathway Analysis knowledgebase was used to analyze the genomic datasets and attribute functional consequences to the effects of related genes, which were significantly up- or down-regulated in response to different treatments. Canonical pathways shown to be significantly modulated at the genomic level in response to exposure included cellular mechanisms, which might contribute to the clinical presentation in GWI patients and thus be targeted for further investigation as potential targets for therapeutic intervention.     

A review and test of predictive models for the bioaccumulation of radiostrontium in fish

Empirical relations between the ⁹⁰Sr concentration factor (CF) and the calcium concentration in freshwater aquatic systems have previously been determined in studies based on data obtained prior to the Chernobyl accident. The purpose of the present research is to review and compare these models, and to test them against a database of post-Chernobyl measurements from rivers and lakes in Ukraine, Russia, Belarus and Finland. It was found that two independently developed models, based on pre-Chernobyl empirical data, are in close agreement with each other, and with empirical data. Testing of both models against new data obtained after the Chernobyl accident confirms the models’ predictive ability. An investigation of the influence of fish size on ⁹⁰Sr accumulation showed no significant relationship, though the data set was somewhat limited.     

Anthropogenic Transformation of Flora in the City of Astrakhan and Its Environs over the Past 100 Years

Trends in the anthropogenic transformation of flora in the city of Astrakhan and its environs over the past 100 years are discussed. In general, the process of flora synanthropization is observed, with natural aboriginal plant communities being replaced by anthropogenically altered communities.     

The transformation of outdoor ammonium nitrate aerosols in the indoor environment

Recent studies associate particulate air pollution with adverse health effects; however, the exposure to indoor particles of outdoor origin is not well characterized, particularly for individual chemical species. We conducted a field study in an unoccupied, single-story residence in Clovis, California to provide data and analyses to address issues important for assessing exposure. We used real-time particle monitors both outdoors and indoors to quantify nitrate, sulfate, and carbon particulate matter of particle size 2.5 μm or less in diameter (PM-2.5). The results show that measured indoor ammonium nitrate concentrations were significantly lower than would be expected based solely on penetration and deposition losses. The additional reduction can be attributed to the transformation indoors of ammonium nitrate into ammonia and nitric acid gases, which are subsequently lost by deposition and sorption to indoor surfaces. A mass balance model that accounts for the kinetics of ammonium nitrate evaporation was able to reproduce measured indoor ammonium nitrate and nitric acid concentrations, resulting in a fitted value of the deposition velocity for nitric acid of 0.56 cm s⁻¹. The results indicate that indoor exposure to outdoor ammonium nitrate in Central Valley of California are small, and suggest that exposure assessments based on total particle mass measured outdoors may obscure the actual causal relationships for indoor exposure to particles of outdoor origin.     

Seasonal Differences in the Levels of Gaseous Air Pollutants in the Vicinity of a Waste Dump

Hydrogen sulphide, ammonia, nitrogen dioxide, mercaptans and sulphur dioxide (H₂S, NH₃, NO₂, R-SH, SO₂) concentrations were measured at the location in the vicinity of the waste dump to determine the air pollution level of these pollutants prior to the operation of the Mobile Thermal Treatment Plant. Samples were collected over one year period. Seasonal differences, and the influence of meteorological parameters (temperature, relative humidity, pressure and wind direction) on the air pollution levels were studied. Results show relatively low concentrations of H₂S, NO₂, R-SH and SO₂, while NH₃ levels were higher compared to the guideline values. Good weather conditions (high air pressure and low relative humidity) are connected to long range transport of NO₂, while higher temperatures result in elevated NH₃ and R-SH concentrations. Because of the predominant northeast wind direction (the same as the waste dump direction), the contribution of air pollution from the direction of the waste dump at the measuring site is significant, but that does not necessarily mean that the pollutants originated from that source.