Biodegradation of endosulfan by a soil bacterium

A bacterium capable of metabolizing endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine3-oxide) was isolated from cotton-growing soil and effectively shown to degrade endosulfan into endosulfan sulfate. The bacterium degraded 50% of the compound within 3 days of incubation. Endosulfan sulfate was the only terminal product and no other metabolites were formed during the incubation. Endosulfan and its metabolites were analyzed by gas chromatography. The metabolites formed indicated that the organism follows an oxidative pathway for metabolism of this pesticide. Therefore, the present study, microbial degradation of endosulfan by a soil bacterium, may provide a basis for the development of bioremediation strategies to remediate the pollutants in the environment.     

The valuation of wetland conservation in an urban/peri urban watershed

This study estimates the social benefits of wetland conservation in the Credit River watershed, located in an urban/peri urban area in Southern Ontario, Canada. A stated preference approach was employed to value wetland conservation programs which ranged from retaining the existing wetlands to restoring various levels of acres of wetlands over the 2009–2020 period. A total of 1,407 households completed an internet-based survey which presented trade-offs in binary choice scenarios framed as referenda. Responses were analyzed using various models, one of which was a latent class analysis which segmented respondents into three classes. This econometric approach uncovered significant preference heterogeneity for wetland conservation. Assignment of respondents to the classes suggested that about one-third of the sample was willing to pay small amounts to retain the existing wetlands. An additional third was willing to pay several hundred dollars a year for retention and small positive amounts for additional restoration. The final third were apparently willing to pay considerable sums for retention, but lesser amounts for additional restoration. However, further analysis revealed that respondents in this third class largely constituted yea-sayers. These results suggest caution in interpreting associated economic valuation estimates and highlight the importance of attempting to understand hypothetical bias in wetland and other such valuation studies.     

Oxidative remobilization of biogenic uranium(IV) precipitates: effects of iron(II) and pH

The oxidative remobilization of uranium from biogenic U(IV) precipitates was investigated in bioreduced sediment suspensions in contact with atmospheric O2 with an emphasis on the influence of Fe(II) and pH on the rate and extent of U release from the solid to the aqueous phase. The sediment was collected from the U.S. Department of Energy Field Research Center (FRC) site at Oak Ridge, Tennessee. Biogenic U(IV) precipitates and bioreduced sediment were generated through anaerobic incubation with a dissimilatory metal reducing bacterium Shewanella putrefaciens strain CN32. The oxidative remobilization of freshly prepared and 1-yr aged biogenic U(IV) was conducted in 0.1 mol/L NaNO3 electrolyte with variable pH and Fe(II) concentrations. Biogenic U(IV)O2(s) was released into the aqueous phase with the highest rate and extent at pH 4 and 9, while the U remobilization was the lowest at circumneutral pH. Increasing Fe(II) significantly decreased U remobilization to the aqueous phase. From 70 to 100% of the U in the sediments used in all the tests was extractable at the experiment termination (41 d) with a bicarbonate solution (0.2 mol/L), indicating that biogenic U(IV) was oxidized regardless of Fe(II) concentration and pH. Sorption experiments and modeling calculations indicated that the inhibitive effect of Fe(II) on U(IV) oxidative remobilization was consistent with the Fe(III) oxide precipitation and U(VI) sorption to this secondary phase.     

Recovery Trajectories After In Situ Burning of an Oiled Wetland in Coastal Louisiana,USA

The high degree of physical disturbance associated with conventional response options to oil spills in wetlands is driving the investigation of alternative cleanup methodologies. In March 1995, a spill of gas condensate in a brackish marsh at Rockefeller Wildlife Refuge in southwestern Louisiana was remediated through the use of in situ burning. An assessment of vegetation recovery was initiated in three treatment marshes: (1) oil-impacted and burned, (2) oil impacted and unburned, and (3) a nonoiled unburned reference. We compared percent cover, stem density, and biomass in the treatment marshes to define ecological recovery of the marsh vegetation and soil hydrocarbon content to determine the efficacy of in situ burning as a cleanup technique. Burning led to a rapid decrease in soil hydrocarbon concentrations in the impacted-and-burned marsh to background levels by the end of the first growing season. Although a management fire accidentally burned the oil-impacted-and-unburned and reference marshes in December 1995, stem density, live biomass, and total percent cover values in the oil-impacted-and-burned marsh were equivalent to those in the other treatment marshes after three years. In addition, plant community composition within the oil-impacted-and-burned marsh was similar to the codominant mix of the grasses Distichlis spicata (salt grass) and Spartina patens (wire grass) characteristic of the surrounding marsh after the same time period. Rapid recovery of the oil-impacted-and-unburned marsh was likely due to lower initial hydrocarbon exposure. Water levels inundating the soil surface of this grass-dominated marsh and the timing of the in situ burn early in the growing season were important factors contributing to the rapid recovery of this wetland. The results of this in situ burn evaluation support the conclusion that burning, under the proper conditions, can be relied upon as an effective cleanup response to hydrocarbon spills in herbaceous wetlands.     

Increased PCDD/F formation in the bottom ash from fires of CCA-treated wood

Bottom ash that was the result of the combustion of chromated copper arsenate (CCA) treated wood under controlled fire conditions showed an increase of several orders of magnitude in the levels of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), compared to that of untreated timber. Wood that has been pressure treated with CCA contains copper (II), which is known to catalyse the so-called de novo formation of PCDD/Fs. Comparable levels of PCDD/Fs would be expected in residual ash from burning CCA-treated wood in backyard fires, stoves and wood heaters, as a consequence of similar combustion conditions.     

Farmers,Streams, Information,and Money: Does Informing Farmers About Riparian Management Have Any Effect?

We assessed relationships between the extent to which farmers reported exposure to relevant information and their attitudes towards, knowledge about, and degree of adoption of riparian management strategies. We also examined associations between knowledge of, or receipt of, financial assistance for riparian fencing/planting and intentions for and extent of adoption of this strategy. A mail survey of 718 pastoral farmers in Otago and Southland in New Zealand [294 surveys returned (41%)] yielded 279 usable questionnaires. Indices were developed to reflect range and frequency of information use and range of practices adopted. Attitudes were measured using Likert-type responses to 11 statements, and knowledge as a score on a ten-question true/false test. Positive relationships between information and the three main response variables (attitude, knowledge, and adoption) were weak but significant and systematic. These associations remained significant when important demographic and farm characteristics were taken into account. Informed farmers were more likely to report intentions to carry out riparian fencing or planting within the next year. Farmers who were aware that funding was available were also more likely to state this intention, independent of information level. The reported extent to which waterways had been fenced to exclude stock was related to receipt of funding, but not to information level. Financial factors were the most influential barrier preventing adoption of permanent fencing. Our research shows a positive correlation between the receipt of information and funding and the adoption of specific riparian management measures.     

Food,feed, and fuelwood resources of Nepal: A GIS evaluation

A GIS-based land evaluation model was developed to determine the food, feed, and fuelwood sufficiency of all districts in Nepal. Resource surpluses or deficits were calculated for each district for 1981, the year for which the national land resource data were available. Of the three resources, feed supplies were found to be the most critical. Feed deficits occurred in 57% of all districts in 1981, while food and fuelwood deficits occurred in less than 10% of all districts. Different scenarios were carried out for the year 2000 to estimate the magnitude of future resource deficits, assuming resource use and yields in Nepal remain constant in the face of growing population and livestock numbers. Linking resource data with spreadsheet and GIS systems provides a new way to understand and evaluate resources at the district and national levels. To more fully capture the usefulness of this approach, information on rates of change in productivity and land use is needed.     

Natural factors and human modifications contributing to marsh loss in Louisiana's Mississippi River Deltaic Plain

Natural factors and human modifications contribute to the estimated annual loss of 10,200 ha of coastal land in the Mississippi River Deltaic Plain Region of south Louisiana. This paper combines information on regional geology and human-induced habitat alterations to evaluate the relative importance of human and natural factors to marsh loss. Data on marsh area and habitat type for 139 7.5-min quadrangles were calculated from maps based on aerial photographs from 1955/56 and 1978, and data on regional geology obtained from published maps were used to construct multivariate model relating initial marsh area, change in urban and agricultural area, change in canal and spoil area, canal area in 1978, depth of sediment overlying the Prairie terrace, and subdelta age to marsh loss. The model indicated that between 25.0% and 39.0% of the marsh loss that occurred during the 23-year period was related to canal and spoil construction, and between 9.5% and 12.7% was related to urban and agricultural development. These are minimal estimates of loss because they do not include many secondary effects (for example, canal orientation, saltwater intrusion, and eutrophication) that can also result in indirect loss. Depth of sediment, initial marsh area, delta lobe age by 1978 canal and spoil area interaction, and indirect effects not included in the model accounted for remaining marsh loss.     

DYNAMICS OF ADDED NITRATE AND PHOSPHATE COMPARED IN A NORTHERN CALIFORNIA WOODLAND STREAM1

ABSTRACT: Injections of NO₃ and PO₄ were made during September 1975 into Little Lost Man Creek, a small pristine stream in Redwood National Park, California. Chloride, a conservative constituent, was added in a known ratio to the nutrients. Nutrient loss at a downstream point was calculated using concentration of added Cl as a reference. Nitrate nitrogen (NO₃-N), added for 4 h, reached 920 μg/1 (above 5 μg/1 background) just below the injection point, but increased only to 405 μg/1 at 310 m downstream. The concentration decrease was attributed to dispersion and to uptake by stream biota. Percent of NO₃-N lost decreased with increasing concentration of NO₃-N. Phosphate phosphorus (PO₄-P) was added a week after the NO₃-N for 3 h, causing a concentration increase of 296 μg/1 (above 13 μg/1 background) just below the injection point, of 161 μg/1 at 90 m downstream, and of 98 μg/1 at 310 m. Percent loss of PO₄-P at downstream sites increased with increasing PO₄-P concentration and also for a short period after peak concentration occurred, but then decreased as PO₄-P concentration continued decreasing. Differences in stream response to added NO₃-N and PO₄-P are attributed to differing rates of reaction with biota and differing degrees of interaction with abiotic stream solids.