Host genotype and age have no effect on rejection of parasitic eggs

Egg rejection belongs to a widely used host tactic to prevent the costs incurred by avian brood parasitism. However, the genetic basis of this behaviour and the effect of host age on the probability of rejecting the parasitic egg remain largely unknown. Here, we used a set of 15 polymorphic microsatellite loci, including a previously detected candidate locus (Ase64), to link genotypes of female great reed warblers (Acrocephalus arundinaceus), a known rejecter, with their egg rejection responses in two host populations. We also tested whether host female age, as a measure of the experience with own eggs, plays a role in rejection of common cuckoo (Cuculus canorus) eggs. We failed to find any consistent association of egg rejection responses with host female genotypes or age. It seems that host decisions on egg rejection show high levels of phenotypic plasticity and are likely to depend on the spatiotemporal variation in the parasitism pressure. Future studies exploring the repeatability of host responses towards parasitic eggs and the role of host individual experience with parasitic eggs would greatly improve our understanding of the variations in host behaviours considering the persistence of brood parasitism in host populations with rejecter phenotypes.     

Reluctant pioneers in the European periphery? Environmental activism,food consumption and “growing your own”

East European food self-provisioning (FSP) has fascinated scholars of post-socialism ever since the early 1990s. In keeping with its predominantly economic and cultural conceptualisations, much of this research has been concerned with FSP’s role in household economy and with the social profile of its practitioners. In contrast to western conceptualisations of FSP as an opportunity to expand food activism and foster social justice and environmental sustainability, post-socialist FSP has rarely been considered as such. In Czechia, FSP is practised by 43% of citizens and many of them do so in a relatively environmentally friendly way. Yet, most food-related campaigns run by environmental NGOs (ENGOs) pay little attention to FSP and focus on market-based ethical consumerism and alternative food networks instead. Using insights from actor-network theory, this paper discusses how Czech ENGO activists engage with FSP through discourse and in practice. Based on ethnographic fieldwork and interviews with leading activists, we show that FSP does figure in non-food-related campaigns and that the FSP practised by activists themselves or the FSP carried out by relatives and relatives’ friends are not the same as the FSP on which they are reluctant to campaign. These differences, which include controllability and the time-consuming nature of practising FSP according to some of the activists’ ideals, help this paper to come to an initial understanding of why Czech ENGOs do not run campaigns explicitly focused on FSP at the moment and shed some light on how this could change in the future.     

Organochlorine compounds in red deer (Cervus elaphus L.) and fallow deer (Dama dama L.) from inland and coastal Croatia

Polychlorinated biphenyl (PCB) and organochlorine pesticide (OCP) levels in fat tissue of red and fallow deer (Cervus elaphus L. and Dama dama L.) from two inland and an Adriatic area were established. Of 17 analysed PCBs, PCB-28, PCB-138, PCB-153, PCB-180 and PCB-118 were found in all samples, whilst PCB-101 and PCB-170 were found in more than 50% of samples. They ranged between 0.03 and 5.98 ng g(-1) fat weight. Of seven analysed OCPs, HCB, γ-HCH and 1,1-dichloro-2,2-di(4-chlorophenyl)ethylene (DDE) were found in all samples, whilst β-HCH was found in more than 50% of samples. They ranged between 0.17 and 22.14 ng g(-1) fat weight. The dominating compounds were DDE, PCB-138, PCB153, PCB-118 and PCB-180. According to the Duncan multistage test, the levels of PCB-138, PCB-153, PCB 170 and PCB-180 were significantly higher in perirenal fat samples of specimens taken from the Adriatic area. DDE was significantly higher in the inland deer samples. Some species differences were determined and were mostly related to higher PCB and β-HCH levels in fallow deer samples and higher DDE levels in red deer samples. No sex difference was established. As for age, significantly higher levels of PCB-118 and PCB-52 were found in fawns.     

Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity

The published results on nanoparticles cytotoxicity and genotoxicity such as titanium dioxide nanoparticles (TiO(2) NPs) are inconsistent, and often conflicting and insufficient. Since different parameters may have impact on the toxicity results, there is need to lay stress on detailed characterization of NPs and the use of different testing conditions for assessment of NPs toxicity. In order to investigate whether dispersion procedures influence NP cytotoxicity and genotoxicity, we compared two protocols giving TiO(2) NP dispersions with different stability and agglomeration states. Detailed primary and secondary characteristics of both TiO(2) NP dispersions in culture media were carried out before toxicological testing; TK6 human lymphoblast cells, EUE human embryonic epithelial cells and Cos-1 monkey kidney fibroblasts were used to assess cytotoxicity (by trypan blue exclusion, proliferation activity and plating efficiency assays) and genotoxicity (by the comet assay). DNA strand breaks were detected by the alkaline comet assay. DNA oxidation lesions (especially 8-oxo-7,8-dihydroguanine, 8-oxoG) were measured with a modified comet assay including incubation with specific repair enzyme formamidopyrimidine DNA glycosylase (FPG). The TiO(2) NPs dispersion with large agglomerates (3 min sonication and no serum in stock solution) induced DNA damage in all three cell lines, while the TiO(2) NPs dispersed with agglomerates less than 200 nm (foetal serum in stock solution and sonication 15 min) had no effect on genotoxicity. An increased level of DNA oxidation lesions detected in Cos-1 and TK6 cells indicates that the leading mechanism by which TiO(2) NPs trigger genotoxicity is most likely oxidative stress. Our results show that the dispersion method used can influence the results of toxicity studies. Therefore at least two different dispersion procedures should be incorporated into assessment of cyto- and genotoxic effects of NPs. It is important, when assessing the hazard associated with NPs, to establish standard testing procedures and thorough strategies to consider the diverse conditions relevant to possible exposures.     

Land degradation due to erosion in public perception. Case study: Secaşul Mare river basin settlements (Transylvanian Depression,Romania)

According to the International Decade for Natural Disaster Reduction (IDNDR 1990–1999), the risk indicates potential losses due to particular natural phenomenon, and these could be reduced by improving of prevention and education. People perceive these losses differently depending on phenomenon occurrence, severity, and impact in time. Starting from this idea, this research presents public perception on land degradation through erosion in a small area from the central part of Romania (south-west of Transylvanian Depression). The research was based on a questionnaire consisting of 16 questions. The items were structured by issues: awareness assessment regarding hazard and risk phenomena, assessment of type of property and land use, assessment of knowledge and information on the possible production of negative effects by natural phenomena, and evaluation of land owners’ attitudes towards the occurrence of erosion on their land. Results reveal that the public perception on erosion is weak. This process is perceived as insignificant due to lack of phenomenon knowledge and especially because of scarcity preoccupation in land’s quality monitoring. Even though the owned lands are affected by erosion forms, the owners are not aware of the phenomenon that generates them. Material damages caused by erosion, loss of soil quality, and land fertility decrease are less perceived because the economic losses fill only at long term. This perception leads to underestimating erosion risk compared to other natural phenomena and to a passive attitude towards this particular phenomenon.     

微藻生物柴油研究现状

本文介绍了微藻生物柴油的优势及技术进展,以及国内外培养微藻制备生物柴油的研究现状,探讨了微藻生物柴油生产关键技术环节及存在的主要问题,展望了微藻生物柴油的未来发展趋势和潜在的应用前景。     

县域生态功能区划研究——以桐柏县为例

结合桐柏县的实例，对县域生态功能区划分的原则和方法进行初步探讨。综合利用3S技术对县域生态敏感性和生态服务功能重要性的分析和评价，提出桐柏县生态服务功能区划的初步方案。将桐柏县划分为生态支持区，产品供给区，生态调节区。简要分析了各功能区在桐柏县经济可持续发展中的地位和作用，指出各区协调发展的方向和途征。     

Distribution of natural radionuclides in surface soils in the vicinity of abandoned uranium mines in Serbia

The activity concentrations of natural radionuclides in soils from the area affected by uranium mining at Stara Planina Mountain in Serbia were studied and compared with the results obtained from an area with no mining activities (background area). In the affected area, the activity concentrations ranged from 1.75 to 19.2 mg kg^?1 for uranium and from 1.57 to 26.9 mg kg^?1 for thorium which is several-fold higher than those in the background area. The Th/U, K/U, and K/Th activity ratios were also determined and compared with data from similar studies worldwide. External gamma dose rate in the air due to uranium, thorium, and potassium at 1 m above ground level in the area affected by uranium mining was found to be 91.3 nGy h^?1, i.e., about two-fold higher than that in background area. The results of this preliminary study indicate the importance of radiological evaluation of the area and implementation of remedial measures in order to prevent further dispersion of radionuclides in the environment.     

我国农业自然资源法的问题与改善途径初探

在介绍我国农业自然资源基本法律体系的基础上,分析了现行法律存在的主要问题,提出了强化农业资源管理的若干建议：①制定农业自然资源综合法律,完善农业自然资源法律体系；②更新充实现行农业自然资源法,以适应社会主义市场经济的要求；③从机制和手段两方面革新管理体制,强化农业自然资源相对统一的综合管理。     

Characteristics of black carbon aerosol mass concentration over the East Baltic region from two-year measurements

Continuous measurements of black carbon (BC) aerosol mass concentration were performed at a background site Preila (55°55'N, 21°00'E, 5 m a.s.l., Lithuania) during the period 2008-2009. The data were used to characterize the BC mass concentration distribution over the East Baltic region. High increase in aerosol BC concentration was associated with the change in air mass characteristics and biomass burning during the winter heating season and spring wildfires. Monthly means of BC concentration ranged from 212 to 1268 ng m(-3) and the highest hourly means of concentration were from 4800 to 6300 ng m(-3), predominantly in spring and winter months. During the October-April period the BC mass concentrations were about twice as high as those in the summertime. The BC diurnal pattern in winter was typically different from that in spring indicating the seasonal variation of the atmospheric boundary layer height. The weekday/weekend difference was not strongly pronounced because the BC concentrations in Preila are mainly affected by long-range transport or local sources. Typical periodicities caused by anthropogenic and meteorological influences have been identified using Fourier analysis. It was shown that domestic heating appears as a 365 day periodicity; traffic slightly contributes 5-7 day peaks in the spectrum and elevated long-range BC can be identified as characteristic peaks with periodicities in the range from 16 to 29 days.Temporal evolution and transport of BC aerosols were interpreted by the air mass backward trajectory analysis in conjunction with the examination of the wavelength dependence on the aethalometer data. Air masses originated from the North Atlantic Ocean and Scandinavia were favourable for lower BC concentrations (350 ng m(-3)), while the BC level associated with the Western Europe airflows was significantly higher (970 ng m(-3)). The mean values of ?ngstr?m exponent of the absorption coefficient (monthly means 1.45 ± 0.25 and 0.84 ± 0.50 over January and June, respectively) revealed that the BC concentration observed over the East Baltic is influenced by submicron sized particles as a result of incomplete biomass combustion during the winter season.