芹菜农药残留监测结果分析

为了解芹菜质量安全现状,对蔬菜批发市场、农贸市场、超市销售的芹菜进行了农药甲胺磷、氧乐果等50项农药的专项抽样调查分析,共抽检芹菜样本213个,通过采用GB2763--2012（食品中农药最大残留限量》进行判定,结果表明：引起芹菜农药残留超标的农药主要为毒死蜱、甲拌磷、多菌灵等,其中由这3种农药引起芹菜超标的比例占农残超标总批次的78．1％,芹菜质量安全状况还需进一步加强。     

贮存寿命评价工具箱设计研究

作为装备贮存可靠性与贮存寿命评价的关键技术,加速寿命试验和加速退化试验已在工程中广泛应用,基于物理分析的贮存寿命特征检测技术也逐步在贮存寿命评价工程中开展。贮存寿命评价技术已有一套完整的理论基础并积累了大量的工程实践经验,但行业内至今仍缺少专门针对贮存寿命评价的软件工具。本文通过对贮存寿命评价工具箱的需求分析研究工作,构建了工具箱的技术框架,并给出工具箱各主要模块的业务流程图和业务概念图,为贮存寿命工具箱的开发提供了支撑。     

Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage

It is observed that ramie (Boehmeria nivea), an economic fiber crop, can establish and colonize metal-contaminated sites in China. Metal tolerance and accumulation by ramie originating from 13 metal-contaminated and 4 “clean” sites in China were compared under field and hydroponic conditions. All selected populations and germplasms displayed good growth performance under diverse metal-contaminated habitats; while growth responses, metal accumulation and tolerance were similar among the 8 populations and 2 germplasms when exposed to solutions containing elevated As, Cd, Pb, or Zn in the laboratory. These revealed that ramie possesses a certain degree of constitutional metal tolerance. To our knowledge, this is the first report of constitutional metal tolerance possessed by a fiber crop. Ramie can be considered as a good candidate for both fiber production and phytoremediation of sites contaminated by multi-metals, as it accumulates relative low metal concentrations, but possesses both high biomass and high economic value.     

Spatial distribution and source identification of persistent pollutants in marine sediments of Hong Kong

A data matrix, obtained during a 3-year monitoring period (2007–2009) from 45 sampling sites in Hong Kong marine, was subjected to determine the spatial characterization and identify the sources of main pollutants. Indicator analyses indicated that polycyclic aromatic hydrocarbons (PAHs), nickel, manganese, and arsenic (As) were at safe levels. Five heavy metals (zinc, lead, cupper, cadmium, chromium (Cr)) were moderate to severe enrichment at some sites. Inner Deep Bay and Victoria Harbor were considered as hot spots for PAHs and the heavy metals, while Tolo Harbor was highly polluted by the heavy metals. Cluster analysis classified the 45 sampling sites into three groups, representing different pollution levels. Principal component analysis/factor analysis identified four principal components (PCs) and explained 84.9 % of the total variances, standing for persistent pollution, N factor, P and Cr factor, and As factor, respectively. Group A was highly polluted by persistent pollution, group B was the less polluted group, and subgroup B1 was less affected by PC3 and PC4 than subgroup B2. Group C, considered as the moderately polluted group, was greatly affected by N factor or persistent pollution, while subgroup C2 received more N pollution than subgroup C1.     

Monitoring method for airborne glymes and its application in fuel exhaust emission measurement.

A monitoring method based on solvent extraction of adsorbed target glymes followed by gas chromatograph-mass spectrometry GC-MS analysis was developed for seven glymes, namely ethylene glycol dimethyl ether, propylene glycol dimethyl ether, ethylene glycol diethyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, triethylene glycol dimethyl ether, and tetraethylene glycol dimethyl ether. The best recoveries of target glymes were achieved when using a combination of sample collection medium of graphitised carbon black (GCB) with a solvent mixture of methylene chloride and methanol (95/5, v/v). Method detection limits ranged from 1.5 microg/m3 for diethylene glycol diethyl ether to 13.2 microg/m3 for ethylene glycol diethyl ether based on a sample volume of 3.4 1. Using this method, diethylene glycol dimethyl ether and diethylene glycol diethyl ether were detected and measured successfully in diluted vehicle exhausts in diesel fuel engine tests.     

Real time alteration of a nuclear waste glass and remobilization of lanthanide into an interphase

The development of predictive models for the long term evolution of nuclear waste glass requires the complete knowledge of the glass dissolution at the laboratory scale. A new approach was developed to determine the initial reaction during the first steps of experience, a new concept was developed, based on the combination of dynamic leaching test and the characterization of the altered materials. With this experimental set-up it is possible to follow in real time the glass alteration process at a fine temporal scale. The results put in evidence a singular behaviour of the lanthanide, shown by a concentration peak of La, Nd and Ce after 2 h and a quick decrease of their concentration measured on line in the solution during the leaching test. This fact is directly linked to the development of an interphase (altered layer which differs from the initial solid by its texture, structure and chemical composition) at the interface of the glass surface and the leaching solution. This work is an attempt to integrate the formation of the alteration products (here the interphase) during leaching into the dissolution mechanisms of a nuclear waste glass. A model is proposed and discussed.     

Application of an enzyme-linked immunosorbent assay for the detection of clenbuterol residues in swine urine and feeds

The present study outlines applications of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol residues. Antisera were raised from rabbits immunized with diazotized clenbuterol-bovine serum albumin (BSA) conjugate. The assay was specific to clenbuterol with a half-maximum inhibition concentration (IC₅₀) of 1.8 ng/mL and 2.5 ng/mL in blank swine urine and phosphate buffer solution, respectively. The assay had high cross-reactivity (86%) with mabuterol, but low with other adrenergic agonists and antagonists. The average recovery of clenbuterol, as measured with the ELISA, ranged from 90% to 112% in swine urine samples and from 86% to 95% in feeds, respectively. This new assay was compared with commercial ELISA test kits. An excellent correlation (r ² = 0.98) between the two methods and satisfactory recoveries suggest that the new assay can be suitable for the determination of clenbuterol residues in real samples. The assay was used to analyze clenbuterol residues in 103 swine urine samples and 68 feed samples collected from northern China. Approximately 50% of the urine samples and 25% of the feed samples analyzed were found positive (concentration of clenbuterol ≥ 1 ppb). The results indicate that clenbuterol was misused in some of the areas surveyed.     

Production of the monoclonal antibody against tylosin for immunoassay of macrolide antibiotics in milk

The objective of the present study was to develop an immunoassay for multi-determination of the residues of four macrolide antibiotics in milk. Tylosin was derivatized with 6-aminohexanoic acid to synthesize a hapten that was used to produce the monoclonal antibody. The obtained monoclonal antibody simultaneously recognized tylosin, acetylisovaleryltylosin, tilmicosin and desmycosin with crossreactivities of 100%, 91%, 49% and 76%, respectively. Then an indirect competitive immunoassay was developed to determine the four analytes in milk simultaneously. The limits of detection for the four analytes were in the range of 5.5–11.7 ng/mL depending on the compound. The recoveries of the four analytes from fortified blank milk were in the range of 78%–96% with coefficients of variation lower than 11.4%. The developed immunoassay could be used as a practical tool for rapid screening the residues of the four anlytes in milk.     

Preparation of Fish-scale Gelatins by Mild Hydrolysis and Their Characterization

This study characterized gelatins prepared by mild hydrolysis of freshwater fish-scale collagen. Among the selected types of protease (trypsin, neutral protease, papain, and alkaline protease), alkaline protease was proven to be the most effective enzyme for gelatin extraction by hydrolysis of fish-scale collagen. The optimum hydrolysis conditions were as follows: reaction time, 6 h; temperature, 50 °C; pH 9; and enzyme amount, 3 % (w/w). Under these optimum hydrolysis conditions, the gelatin yield reached 48.1 % (w/w). The gelatins prepared by alkaline protease hydrolysis show higher emulsion activity and lower emulsion stability indices than those prepared by water extraction.     

Medical waste management in China: A case study of Nanjing

Medical waste management is of great importance due to its infectious and hazardous nature that can cause undesirable effects on humans and the environment. The objective of this study was to analyze and evaluate the present status of medical waste management in the light of medical waste control regulations in Nanjing. A comprehensive inspection survey was conducted for 15 hospitals, 3 disposal companies and 200 patients. Field visits and a questionnaire survey method were implemented to collect information regarding different medical waste management aspects, including medical waste generation, segregation and collection, storage, training and education, transportation, disposal, and public awareness.The results indicated that the medical waste generation rate ranges from 0.5 to 0.8 kg/bed day with a weighted average of 0.68 kg/bed day. The segregated collection of various types of medical waste has been conducted in 73% of the hospitals, but 20% of the hospitals still use unqualified staff for medical waste collection, and 93.3% of the hospitals have temporary storage areas. Additionally, 93.3% of the hospitals have provided training for staff; however, only 20% of the hospitals have ongoing training and education. It was found that the centralized disposal system has been constructed based on incineration technology, and the disposal cost of medical waste is about 580 US$/ton. The results also suggested that there is not sufficient public understanding of medical waste management, and 77% of respondents think medical waste management is an important factor in selecting hospital services.The problematic areas of medical waste management in Nanjing are addressed by proposing some recommendations that will ensure that potential health and environmental risks of medical waste are minimized.