Monoclonal antibody produced by heterologous indirect enzyme-linked immunosorbent assay and its application for parathion residue determination in agricultural and environmental samples

A heterologous indirect enzyme-linked immunosorbent assay (ELISA) was developed, which was based on monoclonal antibody (Mab) to determine parathion residue in agricultural and environmental samples. Eight Mabs were produced by introducing the heterologous indirect ELISA into the screening procedure. It was shown that these Mabs had more broad-reactivity with twenty competitors than that of 5H7 (Mab produced by homologous screening). So it became much easier using these new Mabs to develop heterologous immunoassays. In addition, all the developed heterologous ELISAs could be used to determine parathion residue in semiquantitative or quantitative level, and their detection limitation (LOD) was around 2 ng/mL. Compared with the previous heterologous ELISA (hapten 11/5H7) with IC₅₀ of 13.3 ng/mL, a better heterologous ELISA (hapten 11/1E1) was obtained with IC₅₀ of 3.8 ng/mL, which improved the sensitivity 3.5 times. Finally, the latter was applied to parathion residue determination in spiked agricultural and environmental samples without extraction or cleanup. The average recoveries of parathion added to water, soil, cucumber, Chinese cabbage and carrot were between 80.4 % and 111.8 %. The LOD for water and soil samples was 5 ng/mL, and the LOD for cucumber, rice and corn samples was 10 ng/mL.     

胶东半岛及附近海域地震活动性异常研究

根据胶东半岛及附近海域地震活动性参数的研究,对2012年存在的地震活动性异常演化情况进行跟踪分析,对2013年度出现的新地震异常进行分析,获得大震孕震过程时、空综合变化结果,提出胶东半岛及附近海域的震情趋势判定意见。     

国外二噁污染及有关标准

文章论述了二的化学成分及主要来源 ;二的毒性 ;日本、美国、德国等国二恶英污染状况及各国制定的标准     

铁合金电炉烟尘的性质与低温固结研究

本文研究了硅铁电炉布袋除尘收集的烟尘性能，烟尘低温固结球团的性能和工艺方法。研究结果表明，硅铁电炉烟尘中ＳｉＯ２ 约占９０ － ９５ ％ ，其它组分含量较低，有利于进行回收利用。烟尘密度小、粒度细、表面活性差、成球性能差。采用添加粘结剂进行自然养护和低温固结处理，均能获得强度较好的固结球团，能够加入铁合金炉回收利用。     

公众态度和机构监督对大气污染的影响

虽然以往对于环境库兹涅兹曲线（EKC）的研究中考虑了正式制度因素如政府机构监督，但是较少考虑非正式制度因素.本文重点检验两种制度情境的影响，微观层面的环境态度形成的非正式制度以及宏观层面的环保机构监督形成的正式制度对大气污染排放量的影响.基于CGSS2010-2013和2015年的数据库，从中提取出体现环境态度的问题，将其赋值指标化后，作为制度情境纳入环境方程.经过公开数据的收集，得到我国26个省份2010-2014年5年的面板数据.通过固定效应和随机效应模型，发现机构监督与人均GDP的交互项对氮氧化物、烟（粉）尘、二氧化硫排放都有影响，但是公众态度与人均GDP的交互项只对烟（粉）尘排放有影响，从而对不同制度情境下的环境库兹涅兹曲线进行了探索.     

Using fuzzy method to evaluate safety condition of big diesel engine

Big diesel engine is one of the most important equipments in industry, and its safety condition plays a great role in oil production. This paper presents a fuzzy method to monitor the safety state of the big diesel. Six useful diagnostic characteristic parameters have been selected to diagnose safety condition of the big diesel engine. The standard fuzzy vector was obtained by using statistical method, so the status of big diesel's safety condition can be determined by calculating the fuzzy distance between the fuzzy vector and standard fuzzy vector. This fuzzy method has been successfully used on PZ12V190 diesel engine.     

中德环保合作的高层往来

写在前面的话．本文完成于今年早些时候，但由于刊物在5月时更换出版单位，因而文章在实效性上略有欠缺，但访谈的内容是永久而实际的问题，在当今和未来都是可资借鉴的。     

New fetal cardiac imaging techniques

Rapid advances in graphics computing and micro-engineering have offered new techniques for prenatal cardiac imaging. Some of them can be non-invasively applied to both clinical and laboratory settings, including dynamic three-dimensional echocardiography, myocardial Doppler imaging, harmonic ultrasound imaging, and B-flow sonography. With clinical constraints, a few others have been mainly used in laboratories, such as endoscopic ultrasound, magnetic resonance imaging and biomicroscopy. Appropriate use and co-use of these new tools will not only provide unique information for better clinical assessment of fetal cardiac disease but also offer new ways to improved understanding of cardiovascular development and pathogenesis. Copyright © 2004 John Wiley & Sons, Ltd.