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11.
C. Clark K. F. Kelly N. Smith N. Fairweather T. Brown A. Johnston N. E. Haites 《黑龙江环境通报》1991,11(7):467-470
The polymerase chain reaction has been used to detect an abundant class of short repeat DNA families of the form (dC-dA)n.(dG-dT)n, known as microsatellites. These units are found throughout the human genome and have been characterized for several loci including APOC2 on chromosome 19ql2-ql3.2. The locus APOC2 is linked to the gene for dystrophia myotonica and a microsatellite within this locus was used to derive polymorphisms in a family to predict the inheritance of the disease. Chorionic villus sampling (CVS) was performed at 151/2 weeks' gestation. Following DNA extraction from the CVS material and parental blood samples, microsatellite analysis was carried out by the polymerase chain reaction. 相似文献
12.
Imen Rabeh Khaoula Telahigue Safa Bejaoui Tarek Hajji Lassaad Chouba M’hamed EL Cafsi 《Chemistry and Ecology》2019,35(3):204-218
Mercury (Hg) is a ubiquitous and a major environmental metal pollutant in the aquatic ecosystem. The present study was performed to evaluate the effect of mercury graded doses exposure on oxidative stress, redox status, metallothionein levels and genotoxicity in the intestine of sea cucumber Holothuria forskali. Specimens were exposed for 96?h to three concentrations of Hg (40, 80 and 160?µg/L). Exposure of H. forskali to Hg promoted oxidative stress with an increase in malondialdehyde (MDA), protein carbonyl (PCO) and advanced oxidation protein products (AOPP) levels. An increase of glutathione (GSH), vitamin C (ViteC) and non-protein thiols (NPSH) contents was also observed. Additionally, antioxidant activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) increased especially with the highest doses of Hg indicating a great defense of the antioxidant system. Our investigation revealed an increase in total Metallothionein (MT) content which was more pronounced at the sharpest concentrations of Hg. A significant decline of AChE activity was also observed. In the intestine of Hg-treated H. forskali genotoxicity was confirmed by DNA degradation. 相似文献
13.
This study examines: (1) the impact of two methods of giving screening results on health behaviour and beliefs, evaluated in a randomized controlled trial. (2) The association between uptake of screening and health risk behaviours and beliefs. Two methods of feedback of screening results were given: simple informational feedback, and more extensive feedback, including target-setting and a written contract. At six month follow-up, those who received simple informational feedback had lost less weight and had increased their exercise less than those who received more extensive feedback. Attenders believed more strongly in the relationship between lifestyle and health, perceived themselves as less healthy than others and scored higher on the GHQ than staff who did not attend for screening. These results suggest that belief in the effectiveness of lifestyle change in promoting health, and anxiety about health, can influence uptake of screening. Second, the method of presenting screening results can influence the impact of screening on subsequent behaviour change. 相似文献
14.
Rosanna L. Mattingly Edwin E. Herricks Douglas M. Johnston 《Environmental management》1993,17(6):781-795
The environmental impact of loss of natural stream and riparian habitat is of concern throughout the United States and Europe.
Environmental impacts related to such activities as channelization of and levee construction along streams and rivers are
particularly apparent in the Midwestern United States. The objective of the research presented here was to delineate the extent,
relative degree of impact, and implications for management of channelization and levee construction along watercourses located
in the state of Illinois. According to records maintained through the Illinois Streams Information System data base (Illinois
Department of Conservation), nearly 25% of surface water resources in the state have been modified directly by channelization
and/or levee construction. Reviews of agency records, elaboration of case histories, interviews with agency personnel, and
inspections of impacted sites indicated that these alterations have occurred without the benefit of effective mitigation.
Although permit records may provide suggestions for mitigation to be incorporated in the design of a particular project, permits
issued generally do not require even minimal instream habitat and bank stabilization efforts in conjunction with channel alteration.
Information derived from policy and case study analyses suggests that institutional constraints, rather than lack of particular
understanding about mitigation, provide major barriers to protecting the state's surface water resources in terms of regulatory
review, policy interpretation and implementation, and project evaluation. Recommendations for environmental management efforts
regarding these and similar channel alterations are elaborated from these findings. 相似文献
15.
Adversarial safety analysis: borrowing the methods of security vulnerability assessments 总被引:2,自引:0,他引:2
Johnston RG 《Journal of Safety Research》2004,35(3):245-248
INTRODUCTION: Safety and security share numerous attributes. The author, who heads the (Security) Vulnerability Assessment Team at Los Alamos National Laboratory, therefore argues that techniques used to optimize security might be useful for optimizing safety. OPTIMIZING SECURITY: There are three main ways to attempt to improve security-security surveys, risk assessment (or "design basis threat"), and vulnerability assessments. The latter is usually the most effective. SAFETY ANALOGS: Vulnerability assessment techniques used to improve security can be applied to safety analysis--even though safety is not ordinarily viewed as having malicious adversaries (other than hazards involving deliberate sabotage). Thinking like a malicious adversary can nevertheless have benefits in identifying safety vulnerabilities. SUGGESTIONS: The attributes of an effective safety vulnerability assessment are discussed, and recommendations are offered for how such an adversarial assessment might work. CONCLUSION: A safety vulnerability assessment can potentially provide new insights, a fresh and vivid perspective on safety hazards, and increased safety awareness. 相似文献
16.
Few would now deny that the use of organobromine compounds to achieve fire retardancy in a diverse array of products and materials has led to contamination of the ecosphere on a widespread scale. This environmental prevalence and persistence of the brominated flame retardants, coupled with growing evidence of their potential for harm, present all too familiar parallels with the previous generation of persistent organic pollutants. Indeed, given the intrinsic properties of these brominated chemicals, the nature and extent of the current problem could well have been predicted in advance. The question is then whether we are prepared to let history repeat itself once more or to take precautionary action now to switch to more sustainable alternatives. The choice facing society is not between brominated flame retardants and unsafe products, but between fire safety leading to global contamination or fire safety achieved in less polluting ways. If we look beyond options for simple chemical-for-chemical substitution to alternative materials and designs, many of the solutions are already available. The remainder could undoubtedly be developed given the incentives to do so. However, a strong and clear policy approach, backed by legislative phase-outs within specified (and challenging) timeframes, will be necessary to break our current dependency on organobromine chemistry. This paper presents the justification for such an approach, reviews those initiatives already underway to replace brominated flame retardants and identifies pathways to the use of more sustainable products in the service of society. 相似文献
17.
Molly Johnston Chanelle Warton Mark D. Pertile Michelle Taylor-Sands Martin B. Delatycki Lisa Hui Julian Savulescu Catherine Mills 《黑龙江环境通报》2023,43(2):226-234
Prenatal screening for sex chromosome aneuploidies (SCAs) is increasingly available through expanded non-invasive prenatal testing (NIPT). NIPT for SCAs raises complex ethical issues for clinical providers, prospective parents and future children. This paper discusses the ethical issues that arise around NIPT for SCAs and current guidelines and protocols for management. The first section outlines current practice and the limitations of NIPT for SCAs. It then outlines key guidelines before discussing the ethical issues raised by this use of NIPT. We conclude that while screening for SCAs should be made available for people seeking to use NIPT, its implementation requires careful consideration of what, when and how information is provided to users. 相似文献
18.
Roberts DA Johnston EL Müller S Poore AG 《Environmental pollution (Barking, Essex : 1987)》2008,152(1):153-162
Epifaunal communities associated with macroalgae were exposed to storm water pulses using a custom made irrigation system. Treatments included Millipore freshwater, freshwater spiked with trace metals and seawater controls to allow for the relative importance of freshwater inundation, trace metals and increased flow to be determined. Experimental pulses created conditions similar to those that occur following real storm water events. Brief storm water pulses reduced the abundance of amphipods and gastropods. Freshwater was the causative agent as there were no additional effects of trace metals on the assemblages. Laboratory assays indicated that neither direct nor latent mortality was likely following experimental pulses and that epifauna readily avoid storm water. Indirect effects upon epifauna through salinity-induced changes to algal habitats were not found in field recolonisation experiments. Results demonstrate the importance of examining the effects of pulsed contaminants under realistic exposure conditions and the need to consider ecologically relevant endpoints. 相似文献
19.
Muluken E. Muche Sumathy Sinnathamby Rajbir Parmar Christopher D. Knightes John M. Johnston Kurt Wolfe S. Thomas Purucker Michael J. Cyterski Deron Smith 《Journal of the American Water Resources Association》2020,56(3):486-506
Gridded precipitation datasets are becoming a convenient substitute for gauge measurements in hydrological modeling; however, these data have not been fully evaluated across a range of conditions. We compared four gridded datasets (Daily Surface Weather and Climatological Summaries [DAYMET], North American Land Data Assimilation System [NLDAS], Global Land Data Assimilation System [GLDAS], and Parameter‐elevation Regressions on Independent Slopes Model [PRISM]) as precipitation data sources and evaluated how they affected hydrologic model performance when compared with a gauged dataset, Global Historical Climatology Network‐Daily (GHCN‐D). Analyses were performed for the Delaware Watershed at Perry Lake in eastern Kansas. Precipitation indices for DAYMET and PRISM precipitation closely matched GHCN‐D, whereas NLDAS and GLDAS showed weaker correlations. We also used these precipitation data as input to the Soil and Water Assessment Tool (SWAT) model that confirmed similar trends in streamflow simulation. For stations with complete data, GHCN‐D based SWAT‐simulated streamflow variability better than gridded precipitation data. During low flow periods we found PRISM performed better, whereas both DAYMET and NLDAS performed better in high flow years. Our results demonstrate that combining gridded precipitation sources with gauge‐based measurements can improve hydrologic model performance, especially for extreme events. 相似文献
20.
William Moomaw Lucy Johnston 《Mitigation and Adaptation Strategies for Global Change》2008,13(5-6):615-642
Climate change is creating substantial and growing impacts on the Northeastern United States. As the world’s seventh largest contributor of heat trapping carbon dioxide, the region will play a critical role in moving towards stabilizing global concentrations at a level that avoids serious adverse consequences. The Northeast region is well positioned to be a leader in technology and policy innovation for reducing emissions, and can drive national and international programs that are essential to providing a safer future climate. This paper summarizes technological mitigation options and measures as well as opportunities for public and private actions to reduce emissions. The authors propose a ‘3% solution’ of annual emission reductions to put the Northeastern United States on an emissions reductions path that is consistent with the level of reductions necessary to avoid dangerous climate change. The 3% solution requires a combination of policies that will reduce the energy imbedded in the region’s infrastructure and technologies, and individual action to choose the lowest emitting of available technologies and practices. 相似文献