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151.
Simple molecular method to distinguish the identity of Calanus species (Copepoda: Calanoida) at any developmental stage 总被引:1,自引:0,他引:1
Diagnostic morphological characteristics of copepods of the genus Calanus are restricted largely to minor variations in secondary sex characteristics. This presents a persistent problem in the identification
of individuals to species level, especially for immature stages. We have developed a simple molecular technique to distinguish
between the North Atlantic Calanus species (C. helgolandicus, C. finmarchicus, C. glacialis and C. hyperboreus) at any life stage. Using the polymerase chain-reaction (PCR), the mitochodrial large subunit (16S) ribosomal RNA (rRNA)
gene was amplified from individual copepods preserved in ethanol. Subsequent digestion of the amplified products with the
restriction enzymes DdeI and VspI, followed by electrophoretic separation in 2% agarose (Metaphor, FMC Ltd), produced a characteristic pattern for each species.
The versatility of the method is demonstrated by the unambiguous identification to species of any life stage, from egg to
adult, and of individual body parts.
Received: 11 May 1998 / Accepted: 5 August 1998 相似文献
152.
Kathryn A. Powlen Jonathan Salerno Kelly W. Jones Michael C. Gavin 《Conservation biology》2023,37(4):e14058
Protected areas (PAs) are a commonly used strategy to confront forest conversion and biodiversity loss. Although determining drivers of forest loss is central to conservation success, understanding of them is limited by conventional modeling assumptions. We used random forest regression to evaluate potential drivers of deforestation in PAs in Mexico, while accounting for nonlinear relationships and higher order interactions underlying deforestation processes. Socioeconomic drivers (e.g., road density, human population density) and underlying biophysical conditions (e.g., precipitation, distance to water, elevation, slope) were stronger predictors of forest loss than PA characteristics, such as age, type, and management effectiveness. Within PA characteristics, variables reflecting collaborative and equitable management and PA size were the strongest predictors of forest loss, albeit with less explanatory power than socioeconomic and biophysical variables. In contrast to previously used methods, which typically have been based on the assumption of linear relationships, we found that the associations between most predictors and forest loss are nonlinear. Our results can inform decisions on the allocation of PA resources by strengthening management in PAs with the highest risk of deforestation and help preemptively protect key biodiversity areas that may be vulnerable to deforestation in the future. 相似文献
153.
Assessment of aquatic macroinvertebrates is a critical component of many watershed monitoring programs and passive samplers are often used to collect long-term site data, especially in environments where active sampling is not possible. However, standard passive samplers can be expensive and lost in extreme conditions. We developed a sampler using plastic soda bottles (PSB) filled with river rock and compared its effectiveness with standard Hester-Dendy samplers in both lotic and lentic environments. Abundance, taxa richness, and macroinvertebrate composition showed no significant differences between sampler types in either habitat type. PSB samplers, which can be constructed for less than one dollar each, collected the same number of organisms and represented the same diversity as Hester-Dendy devices that cost around $38 each. In studies where funds are limited, PSB samplers appear to be suitable for passive monitoring. 相似文献
154.
Gwen E. Jones 《组织行为杂志》1999,20(7):1057-1072
Using a 2 × 2 × 2 experimental design and data from 158 subjects, this study assessed the effects of three romance participant characteristics and respondent gender on reactions to working with team members involved in a hierarchical workplace romance. The romance participant characteristics included: (a) marital status of the team leader (married versus single); (b) marital status of a coworker (married versus single); and (c) gender of the team leader–coworker dyad (male–female versus female–male). Significant effects were found for team leader marital status, coworker marital status, and gender of the respondent. This study also examined attributions of blame and attributions of motive (job‐related, ego, or love) to the romantic couple for engaging in the workplace romance. Results indicated that the team leader was more frequently attributed blame, yet attribution of blame was affected by the marital status of both the team leader and the coworker. Both the team leader and the coworker were most frequently attributed an ego motive for the involvement. Implications for work teams and managerial policies are discussed. Copyright © 1999 John Wiley & Sons, Ltd. 相似文献
155.
Food surveys for levels of toxic chemicals frequently report the levels on an uncooked basis. It is known that cooking may in some conditions decrease the amount of dioxins and related chemicals in food. Surveys of polybrominated diphenyl ether (PBDE) levels in food to date show data from uncooked food only. In this article, it was shown that broiling, with fat dripped from the foods reported here, ground beef, ground lamb, catfish, trout, and salmon, reduces the amount of PBDEs in these foods. This suggests that calculations of food intake need to take into consideration levels in the cooked food rather than in the uncooked food. 相似文献
156.
Jonathan E. Naile Jong Seong Khim Jessica N. House Paul D. Jones John P. Giesy 《毒物与环境化学》2013,95(7):1219-1232
It has been reported that the relative response factors of isotopically labeled standards and unlabeled standards of the same perfluorinated compounds could be different. Individual (100 ng mL?1) solutions of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were analyzed using high-performance liquid chromatography tandem mass-spectrometry under negative-ion electrospray to detect any impurities present down to 0.5%–0.1% relative to the major component. Purity of the standards ranged from approximately 86% to ≥ 97%. Standard solutions of unlabeled and isotopically labeled materials were analyzed to compare the response factors of isotopically labeled analytes versus their nonlabeled counterparts in three different matrices at equivalent concentrations: organic solvent (methanol), serum extract, and water present individually and concurrently. Not all labeled analytes have the same response factor as their nonlabeled complement, and in at least one case the matrix in which the standard is present may cause significant suppression of response. Standard solutions of electrochemical fluorination produced PFOA and PFOS were quantified under multiple reaction monitoring mode, using calibration curves prepared from standards consisting primarily of linear standards only. The use of linear only standards may cause under-prediction of concentrations, and that the working range of these standards may be limited. 相似文献
157.
Jennifer L. Jones 《国际发展与全球生态学杂志》2013,20(3):266-278
Conservation is increasingly promoted as a sustainable development instrument in Southern Africa, particularly for remote rural communities. Conservation and development schemes are marketed as community-based projects providing local empowerment through the creation of jobs and cash stemming from protected areas, as well as increased biodiversity protection by local communities whose jobs are dependent on the resource. Transfrontier Conservation Areas (TFCAs), mega Peace Parks that cross international borders, are one of the latest conservation and development paradigms in Southern Africa. TFCAs have gained broad support, including government recognition as a development tool. However, there has been minimal research on the impact of TFCAs on local communities. This paper seeks to provide an empirical case study of a South African community bordering the Lubombo TFCA (South Africa, Swaziland, Mozambique). Results are presented that indicate the Mbangweni community in KwaZulu-Natal could experience decreased access to social, natural, and economic resources as a result of the Peace Park. 相似文献
158.
Hamede R Lachish S Belov K Woods G Kreiss A Pearse AM Lazenby B Jones M McCallum H 《Conservation biology》2012,26(1):124-134
Pathogen-driven declines in animal populations are increasingly regarded as a major conservation issue. The Tasmanian devil (Sarcophilus harrisii) is threatened with extinction by devil facial tumor disease, a unique transmissible cancer. The disease is transmitted through direct transfer of tumor cells, which is possible because the genetic diversity of Tasmanian devils is low, particularly in the major histocompatibility complex genes of the immune system. The far northwest of Tasmania now holds the last remaining disease-free wild devil populations. The recent discovery of unique major histocompatibility complex genotypes in the northwestern region of Tasmania has raised the possibility that some animals may be resilient to the disease. We examined the differences in the epidemiology and population effects of devil facial tumor disease at 3 well-studied affected sites in eastern Tasmania and 1 in western Tasmania (West Pencil Pine). In contrast to the 3 eastern sites, there has been no rapid increase in disease prevalence or evidence of population decline at West Pencil Pine. Moreover, this is the only onsite at which the population age structure has remained unaltered 4 years after the first detection of disease. The most plausible explanations for the substantial differences in population effects and epidemiology of the disease between eastern and western sites are geographic differences in genotypes or phenotypes of devils and functional differences between tumor strains in the 2 regions. We suggest that conservation efforts focus on identifying whether either or both these explanations are correct and then, if resistance alleles exist, to attempt to spread the resistant alleles into affected populations. Such assisted selection has rarely been attempted for the management of wildlife diseases, but it may be widely applicable. 相似文献
159.
Substantial improvements in residential energy efficiency are required if the UK is to meet its target of reducing CO 2 emissions by 20% between 1990 and 2010. The Home Energy Conservation Act 1995 (HECA) devolves much of the responsibility for this to local authorities, by requiring them to draw up strategies to increase energy efficiency in this sector by 30% in the next 10-15 years. While many authorities have responded positively to the Act, others are struggling, and 25% of HECA reports initially failed to meet the Act's statutory obligations. The Association for the Conservation of Energy (ACE) conducted research to identify barriers faced by local authorities in implementing HECA. They were found to include officers' lack of experience, their lack of time and a lack of support from elected members. Action must be taken to ensure that HECA achieves its potential. The authors suggest that the Act needs to be adequately supported by central legislation, and that devolution of responsibility must be accompanied by some devolution of finances. The authors also recommend that the Act could be used as an example for international replication. 相似文献
160.
Jones S 《Journal of environmental radioactivity》2008,99(1):1-6
The autumn of 2007 has seen the fiftieth anniversary of two separate events that were of great importance both for the worldwide nuclear industry and for the science of radioecology. This short article marks this anniversary by briefly reviewing the causes, releases, impacts and legacies of both the Kyshtym accident of September 1957 and the Windscale accident of October 1957. 相似文献