Effect of arm regeneration on energy storage and gonad production in Ophiocoma echinata (Echinodermata: Ophiuroidea)

Two laboratory experiments were conducted on Ophiocoma echinata (Lamarck, 1816) collected from Missouri Key, Florida, USA, to ascertain the effects of arm regeneration on energy storage and gonad production. In each experiment individuals were divided into non-regenerating (all arms intact) and regenerating (three arms autotomized) groups and maintained together in a re-circulating seawater system for 2 mo. In Experiment 1, individuals were fed once a week, and in Experiment 2 once every 2 wk. The amount of food energy required for maintenance was estimated to be 0.172 kJ d⁻¹. The lower feeding level (Experiment 2) was below maintenance ration and both groups lost material relative to an initial group. At the higher feeding level (Experiment 1), regeneration of three arms resulted in less storage of organic material, mainly lipid in the stomach, and less gonad production. Thus, regeneration may reduce energy reserves and reproductive output. Received: 23 April 1998 / Accepted: 11 January 1999     

Preimplantation genetic diagnostic protocols for α- and β-thalassaemias using multiplex fluorescent PCR

Haemoglobinopathies including α- and β-thalassaemia are the world's most common class of single gene disorder. Prenatal diagnosis (PND) for β-thalassaemia has been proven to be an effective strategy for controlling the incidence of new cases and is widely used in several countries where the disease is common. Successful preimplantation genetic diagnosis (PGD) protocols for β-thalassaemia have been introduced using restriction fragment length polymorphism (RFLP), single-stranded conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE). However, contamination and allele dropout (ADO) remain an important concern for all of these strategies. In the present study two PGD protocols for detecting β-thalassaemia mutations (codon 41-42 and IVSI-110) and one for α-thalassaemia (SEA mutation) have been designed and tested. These methods contain failsafe mechanisms to reduce the risk of misdiagnosis due to ADO or contamination and utilise multiplex fluorescent PCR (F-PCR). Interestingly, amplification efficiency and ADO were significantly affected by the choice of DNA polymerase and the freshness of the single cells used. The close similarity between the DNA sequences of β-globin and δ-globin was also found to be an important issue that necessitated careful design of primers for the β-globin gene. Copyright © 2001 John Wiley & Sons, Ltd.     

Prenatal diagnosis of multiple acyl-CoA dehydrogenase deficiency: association with elevated α-fetoprotein and cystic renal changes

We report the occurrence of multiple acyl-CoA dehydrogenase deficiency (MADD) in two consecutive pregnancies in a young, Caucasian, non-consanguineous couple. In the first pregnancy, the maternal serum α-fetoprotein was elevated. A sonogram showed growth delay, cystic renal disease, and oligohydramnios; the parents decided to terminate the pregnancy. Postmortem examination confirmed the cystic renal disease and showed hepatic steatosis, raising the suspicion of a metabolic disorder. The diagnosis of MADD was made by immunoblot studies on cultured fibroblasts. In the subsequent pregnancy, a sonogram at 15 weeks' gestation showed an early growth delay but normal kidneys. The maternal serum and amniotic fluid concentrations of α-fetoprotein were elevated, and the amniotic fluid acylcarnitine profile was consistent with MADD. In vitro metabolic studies on cultured amniocytes confirmed the diagnosis. A follow-up sonogram showed cystic renal changes. These cases provide additional information regarding the evolution of renal changes in affected fetuses and show a relationship with elevated α-fetoprotein, which may be useful in counseling the couple at risk. MADD should be considered in the differential diagnosis of elevated α-fetoprotein and cystic renal disease. Early growth delay may be an additional feature. Copyright © 2001 John Wiley & Sons, Ltd.     

Forests in common: Learning from diversity of community forest arrangements in Europe

Europe has a wealth of community forest arrangements. This paper aims to transcend the diversity of locally specific terms and forms, to highlight the value of considering them inclusively. Building on methods to make sense of diversity, we use reflexive grounded inquiry in fifteen cases in Italy, Scotland, Slovenia and Sweden. Within four dimensions (forest, community, relationships between them, and relationships with wider society), we identify 43 subdimensions to describe them collectively. Our approach shows how European arrangements contribute to wider discourses of collective natural resource management. Both tradition and innovation in Europe inform options for environmental governance. Arrangements challenge the distinction between ‘communities of place’ and ‘communities of interest’, with implications for social and environmental justice. They exemplify multilevel environmental governance through both vertical and horizontal connections. Emerging from long histories of political and environmental pressures, they have a role in enhancing society’s connection with nature and adaptive capacity.Electronic supplementary materialThe online version of this article (10.1007/s13280-020-01377-x) contains supplementary material, which is available to authorised users.     

Relationships among Isolated Wetland Size, Hydroperiod, and Amphibian Species Richness: Implications for Wetland Regulations

Abstract: Wetland development within the United States is regulated primarily by size. Decisions concerning wetland destruction or conservation are therefore based in part on three inherent assumptions: (1) small wetlands contain water for short portions of the year; (2) small wetlands support few species; and (3) species found in small wetlands are also found in larger wetlands. We tested these assumptions using data on wetland size, relative hydroperiod (drying scores), and relative species richness of amphibians in depression wetlands of the southeastern United States. We found a significant (p = 0.03) but weak (r² = 0.05) relationship between hydroperiod and wetland size and no relationship (p = 0.48) between amphibian species richness and wetland size. Furthermore, synthetic models of lentic communities predict that short-hydroperiod wetlands support a unique group of species. Empirical investigations support this prediction. Our results indicate that hydroperiod length should be included as a primary criterion in wetland regulations. We advocate a landscape approach to wetlands regulation, focused in part on conserving a diversity of wetlands that represent the entire hydroperiod gradient.     

Blood-based biomarkers in the maternal circulation associated with fetal growth restriction

Fetal growth restriction (FGR) is associated with threefold to fourfold increased risk of stillbirth. Identifying FGR, through its commonly used surrogate—the small-for-gestational-age (SGA, estimated fetal weight and/or abdominal circumference <10th centile) fetus—and instituting fetal surveillance and timely delivery decrease stillbirth risk. Methods available to clinicians for antenatal identification of SGA fetuses have surprisingly poor sensitivity. About 80% of cases remain undetected. Measuring the symphysis-fundal height detects only 20% of SGA fetuses, and even universal third trimester ultrasound detects, at best, 57% of those born SGA. There is an urgent need to find better ways to identify this at-risk cohort. This review summarises efforts to identify molecular biomarkers (proteins, metabolites, or ribonucleic acids) that could be used to better predict FGR. Most studies examining potential biomarkers to date have utilised case-control study designs without proceeding to validation in independent cohorts. To develop a robust test for FGR, large prospective studies are required with a priori validation plans and cohorts. Given that current clinical care detects 20% of SGA fetuses, even a screening test with ≥60% sensitivity at 90% specificity could be clinically useful, if developed. This may be an achievable aspiration. If discovered, such a test may decrease stillbirth.     

Staying in place during times of change in Arctic Alaska: the implications of attachment,alternatives, and buffering

The relationship between stability and change in social-ecological systems has received considerable attention in recent years, including the expectation that significant environmental changes will drive observable consequences for individuals, communities, and populations. Migration, as one example of response to adverse economic or environmental changes, has been observed in many places, including parts of the Far North. In Arctic Alaska, a relative lack of demographic or migratory response to rapid environmental and other changes has been observed. To understand why Arctic Alaska appears different, we draw on the literature on environmentally driven migration, focusing on three mechanisms that could account for the lack of response: attachment, the desire to remain in place, or the inability to relocate successfully; alternatives, ways to achieve similar outcomes through different means; and buffering, the reliance on subsidies or use of reserves to delay impacts. Each explanation has different implications for research and policy, indicating a need to further explore the relative contribution that each makes to a given situation in order to develop more effective responses locally and regionally. Given that the Arctic is on the front lines of climate change, these explanations are likely relevant to the ways changes play out in other parts of the world. Our review also underscores the importance of further attention to the details of social dynamics in climate change impacts and responses.