Variation in the absorption of macromolecular proteins in larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase

Absorption of proteins by pinocytosis was studied in the postvalvular intestine of larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase. Comparison of specimens ranging in age from 4 or 5 d (age of mouth opening) to 25 d (period of strictly exotrophic feeding) made it possible to determine the morphofunctional development of enterocytes when fish are fed onArtemia sp. (Brazil strain). The pinocytotic activity of enterocytes was weak at the time of mouth opening, but intensified rapidly. After the first feed (7 to 8 d), enterocytes were capable of absorbing proteins, since they actively incorporated ferritin. At the age of 18 to 19 d, they showed a strong capacity for absorption resulting in the formation of a large supranuclear vacuole by the age of 25 d. Examination of the intestine of larvae fed artificially showed that enterocytes developed little until 18 to 19 d of age, but the presence of supranuclear vacuoles in the cytoplasm nevertheless indicated a capacity for absorption.     

CHANNEL NETWORK EXTENSION BY LOGGING ROADS IN TWO BASINS,WESTERN CASCADES,OREGON1

ABSTRACT: Based on field surveys and analysis of road networks using a geographic information system (GIS), we assessed the hydrologic integration of an extensive logging-road network with the stream network in two adjacent 62 and 119 km² basins in the western Cascades of Oregon. Detailed surveys of road drainage for 20 percent of the 350 km road network revealed two hydrologic flow paths that link roads to stream channels: roadside ditches draining to streams (35 percent of the 436 culverts examined), and roadside ditches draining to culverts with gullies incised below their outlets (23 percent of culverts). Gully incision is significantly more likely below culverts on steep (< 40 percent) slopes with longer than average contributing ditch length. Fifty-seven percent of the surveyed road length is connected to the stream network by these surface flowpaths, increasing drainage density by 21 to 50 percent, depending on which road segments are assumed to be connected to streams. We propose a conceptual model to describe the hydrologic function of roads based on two effects: (1) a volumetric effect, increasing the volume of water available for quickflow and (2) a timing effect, altering flow-routing efficiency through extensions to the drainage network. This study examines the second of these two effects. Future work must quantify discharge along road segments connected to the stream network in order to more fully explain road impacts on basin hydrology.     

PHOSPHORUS ASSIMILATION IN A STREAM SYSTEM OF THE LAKE OKEECHOBEE BASIN1

ABSTRACT: The ability to predict how streams and wetlands retain phosphorus (P) is critical to the management of watersheds that contribute nutrients to adjacent aquatic systems such as lakes. Field and laboratory experiments were conducted to determine the P assimilatory capacity of a stream (Otter Creek) in the Taylor Creek/Nubbin Slough Basin located north of Lake Okeechobee, Florida. Dominant soils in this basin are sandy Spodosols; landuse is primarily dairy farms and beef cattle pastures. Estimates of P assimilation show that sediments assimilate approximately 5 percent of the P load. Phosphorus assimilation rates in the stream were estimated using first-order relationships based on the total P concentration of the water column as a function of distance from the primary source. This method assumes minimal lateral inputs. Stream lengths required for one turnover in P assimilation were estimated to be in the range of 3–16 km. Laboratory studies using intact sediment cores indicated a P assimilation rate of 0.025 m day^?1, and equilibrium P concentration of 0.16 ± 0.03 mg L^?1 in the water column. Dissolved P concentration gradients in the sediments showed upward flux of P at water column P concentration of <0.16 mg L^?1. Approximately 56–77 percent of the P assimilated in the above-ground vegetation during active growth was released or translocated within six months of senesence, suggesting short-term storage in above-ground vegetation. Bottom sediments and recalcitrant detrital plant tissue provide for long-term P assimilation in the creek. Although stream sediments have the potential to adsorb P, high flow rate and low contact period between water and sediment limits this process.     

Detection and Molecular Characterization of Gemycircularvirus from Environmental Samples in Brazil

Gemycircularvirus (GemyCV) is a group of viruses which has been recently proposed as a new viral genus detected in fecal and environmental samples around the world. GemyCVs have been detected in human blood, brain tissue, cerebrospinal fluid, and stool sample. In the present study, we demonstrate for the first time, through molecular detection and characterization, the presence of GemyCVs in environmental samples from Brazil. Our results show a percentage of positivity ranging from 69 (25/36) to 97 % (35/36) in river water samples collected in Manaus, Amazon region, and wastewater from a wastewater treatment plant located in Rio de Janeiro, respectively, revealing GemyCVs as an important environmental contaminant.     

Seeding agroecology through new farmer training in Canada: knowledge,practice, and relational identities

As a concept, agroecology emphasises the interweaving of scientific and traditional ecological knowledge and is evolving in conjunction with farmer-led social movements from around the world addressing the health, equity and ecological sustainability of food systems. In Canada, many new agroecological farmers come from non-farming backgrounds and are finding limited training opportunities and support structures. While there is a growing literature on the evolution of agroecology, there is limited research on the existence and impact of training programmes on the subject-formation of new farmers. In this paper, we consider the subject-formation of new agroecological farmers through a case study of the Everdale Community Learning Centre, one of Canada’s only agroecological farm schools. In particular, we explore how the knowledge, practice, and relational identities of participating graduates are informed by and build on the science, practice, and movement of agroecology. Drawing on a survey and interviews with past participants, we found that Everdale’s education programme contributes to an agroecological subject-formation by promoting the co-creation of place-based agricultural knowledge; teaching the complexities of agroecology practice through both experiential and theoretical training; and, building a supportive community of peers. We conclude with reflections on ways to encourage a greater diversity of new farmer entrants and opportunities to support training programme graduates in establishing successful farms. These findings provide insight into developing new agroecological farmers and supporting the growing agroecological movement in Canada.     

Nitrogen critical loads for alpine vegetation and terrestrial ecosystem response: are we there yet?

Increases in the deposition of anthropogenic nitrogen (N) have been linked to several terrestrial ecological changes, including soil biogeochemistry, plant stress susceptibility, and community diversity. Recognizing the need to identify sensitive indicators of biotic response to N deposition, we empirically estimated the N critical load for changes in alpine plant community composition and compared this with the estimated critical load for soil indicators of ecological change. We also measured the degree to which alpine vegetation may serve as a sink for anthropogenic N and how much plant sequestration is related to changes in species composition. We addressed these research goals by adding 20, 40, or 60 kg N x ha(-1) x yr(-1), along with an ambient control (6 kg N x ha(-1) x yr(-1) total deposition), to a species-rich alpine dry meadow for an eight-year period. Change in plant species composition associated with the treatments occurred within three years of the initiation of the experiment and were significant at all levels of N addition. Using individual species abundance changes and ordination scores, we estimated the N critical loads (total deposition) for (1) change in individual species to be 4 kg N x ha(-1) yr(-1) and (2) for overall community change to be 10 kg N x ha(-1) x yr(-1). In contrast, increases in NO3- leaching, soil solution inorganic NO3-, and net N nitrification occurred at levels above 20 kg N x ha(-1) x yr(-1). Increases in total aboveground biomass were modest and transient, occurring in only one of the three years measured. Vegetative uptake of N increased significantly, primarily as a result of increasing tissue N concentrations and biomass increases in subdominant species. Aboveground vegetative uptake of N accounted for <40% of the N added. The results of this experiment indicate that changes in vegetation composition will precede detectable changes in more traditionally used soil indicators of ecosystem responses to N deposition and that changes in species composition are probably ongoing in alpine dry meadows of the Front Range of the Colorado Rocky Mountains. Feedbacks to soil N cycling associated with changes in litter quality and species composition may result in only short-term increases in vegetation N pools.     

Assimilation of dissolved glucose from sea water by the sea bass Dicentrarchus labrax and the sea bream Sparus aurata

Larvae of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) were transferred to normal or glucose-enriched sea water immediately after mouth opening to assess their ability to absorb and assimilate glucose at the beginning of the larval period. Assimilation was monitored by histological and cytochemical analysis of the liver. The results showed that (1) the larvae of both species regularly ingested water, (2) glucose absorption resulted in glycogen accumulation in the hepatocytes (this was more marked in sea bass than in sea bream), and (3) glucose delayed the pathological effects of fasting. Consideration of metabolic derivatives indicates that hepatic glycogen probably arises from neoglucogenesis.     

Larval development of Ocypode quadrata (Brachyura: Crustacea) under laboratory conditions

The larvae of Ocypode quadrata (Fabricius) have been reared in the laboratory, from hatching to megalopa stage, at 35 S, 25°C. The five zoeal stages and the megalopa are described, including functional appendages of each stage. On the basis of morphological characteristics, the first zoeal and megalopa stages of O. quadrata can be distinguished from similar stages of closely related Ocypodinae. At 25°C, the megalopa appeared in a minimum of 34 days following hatching.     

Bisexually bonded ranging in chimpanzees (Pan troglodytes verus)

Abstact While male mammals seek to maximize access to potential mates, females maximize feeding efficiency. Ranging patterns are therefore often sex specific. Sex-specific ranging patterns have also been reported for East African chimpanzees and a recent study on female ranging patterns concludes that social organization is best described by a male-bonded community model, where females occupy individual home ranges that are distributed within the boundaries of the male-defended range. In West African chimpanzees, however, such sex-specific ranging patterns have not been consistently observed and a bisexually bonded community model, where both sexes use the entire home range equally, has been suggested to best describe social organization. In this study we analyze 5 years of data on individual ranging patterns of chimpanzees in the Taï National Park (Côte dIvoire) to test specific predictions of the different models of chimpanzee social organization. We found that although males in Taï had slightly larger home ranges than females, all individual home ranges and core areas overlapped highly. Small individual home range size differences were entirely due to the use of peripheral areas and were correlated with female social dominance. These findings strongly support the bisexually bonded community model for Taï chimpanzees. Thus, we conclude that there are fundamental differences in the space-use patterns of East and West African chimpanzees and discuss possible factors leading to such differences.Communicated by D. Watts