The meta-hydroxylation step in the phenylpropanoid pathway: a new level of complexity in the pathway and its regulation

Together in silico and genetic mining approaches have recently designated the CYP98 family of plant cytochromes P450 as the family of enzymes that catalyzes the meta-hydroxylation step in the phenylpropanoid pathway. This meta-hydroxylation is not catalyzed on the free p-coumaric acid as anticipated, but on its conjugates with shikimic, quinic, or phenyllactic acids. While all CYP98s have in common phenol meta-hydroxylase activity, p-coumaroylshikimate remains their preferred substrate. High expression of CYP98s is detected in lignifying tissues in stems, roots, and siliques. The CYP98A3 gene disruption in Arabidopsis thaliana leads to a drastic inhibition of lignin synthesis, cell growth, and plant development. The meta-hydroxylation of phenolic precursors is thus essential for higher plant development. Isolation of coding sequences belonging to the CYP98 family from basil, wheat, and extensive functional analysis of the recombinant enzymes, together with CYP98s from other plant taxa, helps shedding some light on mechanisms of P450s evolution. Most importantly, the occurrence of the meta-hydroxylation on esters of shikimic or quinic acids introduces a new biochemical regulation mechanism in the phenylpropanoid pathway.     

When does quality of relationships with coworkers predict burnout over time? The moderating role of work motivation

The present prospective study examines the interplay between the quality of relationships with coworkers and work motivation in predicting burnout. Considering self-determined motivation at work as a potential moderator, we investigated whether relationships with coworkers are equally important to all employees in preventing burnout. A total of 533 college employees participated in this study. Data were collected at two time points, two years apart. Results from structural equation modeling indicated negative main effects for high-quality relationships and self-determined motivation on burnout. A significant interaction effect between these two factors on burnout was also revealed, suggesting that high-quality relationships with coworkers is crucial for those employees who exhibit less self-determined work motivation. Implications for burnout research and management practices are discussed (Deci & Ryan, 1985 ). Copyright © 2009 John Wiley & Sons, Ltd.     

The assessment of electromigration as a new technique to study diffusion of radionuclides in clayey soils

For the performance assessment study of a geological disposal of High Level Waste (HLW) in clayey formations, migration studies are essential. For low permeability soils (clays), classical diffusion studies take a very long time. In order to reduce the experimental time, we propose an electrical field as driving force to accelerate the migration of ionic species. This paper reports the assessment of the electromigration technique as a powerful new and fast technique for migration studies. The apparent molecular diffusion coefficient can be derived by two independent methods using the migration parameters obtained from an electromigration experiment, namely the apparent dispersion coefficient and the apparent convection velocity. First, it can be calculated from the velocity of the migrating species by the Einstein relation. But, corrections are necessary for electroosmotic flow. The apparent electroosmotic mobility is experimentally determined as 2.2·10⁻⁹ m²/Vs. Second, it can be calculated from the relation between the apparent dispersion coefficient and the total apparent convection velocity. But it is necessary to know the dispersion length of the medium. The dispersion length for Boom Clay is experimentally determined as 8·10⁻⁵ m. Because of the serious reduction in time, it becomes possible to run series of experiments at different electrical fields to obtain averaged values for the apparent molecular diffusion coefficient according to the two methods. Experiments at different electrical fields have another advantage: the intercept of the linear relationship between the total apparent convection velocity and the apparent dispersion coefficient gives the apparent molecular diffusion coefficient. The apparent molecular diffusion coefficients obtained for ⁸⁵Sr, ¹³¹I and HTO are respectively 0.8·10⁻¹¹, 15·10⁻¹¹, and 24·10⁻¹¹ m²/s. These values are confirmed by pure diffusion experiments. The excellent agreement with the apparent molecular diffusion coefficients obtained by classical diffusion tests clearly demonstrates the feasibility of the electromigration technique for the determination of diffusion coefficients.     

Gender roles and economics of exploitation,processing and marketing of bivalves and impacts on forest resources in the Sanaga Delta region of Douala-Edea Wildlife Reserve,Cameroon

Socio-economic surveys were carried out in the Malimba and Mouanko communities in the Sanaga delta region of the Douala-Edea Wildlife Reserve, Cameroon, on the exploitation, processing and marketing of bivalves, with emphasis on gender aspects and impacts on the surrounding forest resources. Sixty people from 16 villages were sampled using a structured questionnaire with field observations documented during the peak fishing period (November 2000–June 2001). Results indicated a strong gender differentiation in the activity, with males dominating bivalve harvesting and shell processing and women dominating the processing for meat. Monthly bivalve meat production of 32.9 tons (197.4 tons per year) generated revenues of 65.4 million FCFA (74.9 million per year). Monthly shell production was put at 1380 tons (8040 tons per year) generating a monthly revenue of 74 million FCFA (444 million per year). Problems faced during exploitation, processing and marketing stages include physical, health and socio-economic: use of very depreciable rudimentary tools, shell injuries, fire and hot water burns, insect pests; blindness, deafness; inadequate storage facilities, transport and remoteness of the area. Bivalve processing activities using wood contributed to a total annual harvest of 50 986 m³ from the surrounding forest. Sustainable conservation measures geared towards poverty alleviation are proposed.