Prenatal diagnosis of chinese homozygous α-thalassaemia 1 and haemoglobin H disease by analysis of α- and φζ-globin genes in chorionic villi and amniocytes

Eighty-eight high-risk pregnancies, 81 for homozygous α-thalassaemia 1 and 7 for haemoglobin (Hb) H disease, were collected in this study. Chorionic villus sampling (CVS) was done in 63 cases and amniocentesis in 25 cases to obtain fetal cells. Southern blotting and DNA hybridization with α- and φζ-globin gene probes were used to determine the α-globin gene status. In two non-informative families with non-deletional mutations, DNA analysis failed to rule out the affected condition, and fetal blood sampling (FBS) and Hb electrophoresis were used for the final diagnosis. In the 81 fetuses at risk for homozygous α-thalassaemia 1, 17 (13 by CVS and 4 by amniocentesis) were afffected, 30 were α-thalassaemia 1 heterozygotes, 19 were normal, and the remaining 15 were either normal or heterozygous. In the seven fetuses at risk for Hb H disease, one was normal, three were α-thalassaemia 1 heterozygotes, two were α-thalassaemia 2 heterozygotes, and one was affected with Hb H disease and developed hydrops fetalis. DNA analysis on fetal cells enabled us to diagnose prenatally severe α-thalassaemias, to prevent the birth of infants with Hb H disease, and to minimize maternal obstetrical complications from harbouring a fetus with Hb Bart's hydrops fetalis.     

Experiences of Coal Ash Artificial Reefs in Taiwan

A feasibility study for using fly-ash from Taiwan coal-fired power stations for artificial reef production was started in 1983. Various mixtures of fly ash stabilized with lime, cement, or industrial wastes and formed into blocks were tested in the laboratory. the results showed that the development of compressive strength of those blocks immersed in the sea water was much better than those exposed in the air. Heavy metal (Cd, Cr, Cu, Ni, Pb, Zn) content of the blocks has been monitored routinely to determine the leaching rates. the result indicates that solidification of fly-ash could indeed immobilize heavy metals better than the original fly ash. From March 1984 to February 1987, 3,682 coal ash stabilized blocks with total weight of 300 tons were designed, fabricated and installed at both Hsin-ta, south western coast, and Kuei-hou, northern coast of Taiwan, for field trials. After four years' underwater observations on 100 cubic blocks at Wan-Li, northern Taiwan, it was shown that the physical integrity of the ash reef blocks had been maintained. the compressive strength and durability was better than the concrete artificial reefs nearby. the ash reefs could also attract fishes and the colonization by benthic organisms was similar to that on concrete reefs. Eighty-eight species of fishes were observed, of which 27 were commercially important, comprising more than 80% of the total biomass. These results demonstrate a potential use for stabilized coal ash as artificial reefs to replace traditionally used concrete reefs to protect and enhance the coastal fishery resources in Taiwan in the future.     

Applying hazardous substance management to supplier selection using analytic network process

With increased outsourcing and environmental consciousness, this paper presents an analytic network process (ANP) approach to incorporate the issue of hazardous substance management (HSM) into supplier selection. In this study, identification of criteria of HSM competence is categorized into four dimensions, a multi-criteria decision model is proposed. ANP is then applied to supplier selection and is characterized by interdependencies among decision structure components. An illustrative example in an electronics company is presented to demonstrate how to select a most appropriate supplier in accordance with the requirements of hazardous substance for environmental regulations.     

INFLUENCE OF BATHYMETRIC CHANGES ON HYDRODYNAMICS AND SALT INTRUSION IN ESTUARINE SYSTEM1

ABSTRACT: A vertical (laterally integrated) two‐dimensional numerical model has been applied to study the hydrodynamic characteristics and salt water intrusion in the Tanshui River estuarine system. The cross‐sectional profiles measured in 1978 and 1994 are schematized for model simulations. Detailed model calibration and verification have been conducted with water surface elevations, tidal current, salinity distributions, and residual velocities measured. The overall performance of the model is in qualitative agreement with the available field data. The model was then used to study how hydrodynamics and salt water intrusion change in response to changes in bathymetry. The model simulations indicate that more tidal energy propagates into the estuarine system in 1994 because of the substantial increase in river cross‐sections. The limits of salt intrusion in 1994 extended farther inland than those in 1978. On the other hand, the extent of mangrove wetland in the lower estuary has increased over the past 20 years and is likely a result of the increased salinity in the estuary.     

Modelling the propagation of an internal solitary wave across double ridges and a shelf-slope

Evolution of the internal solitary waves (ISWs) in the northern South China Sea (SCS) has recently attracted the attention of many oceanographers in Taiwan and the United States. These ISWs are believed to have been induced by a branch of the Kuroshio current over Luzon Strait, which propagates westward over two ridges in the Luzon Strait between Taiwan and the Philippines, and further onto the continental margin with a shelf-slope in the SCS. This paper presents some preliminary results for the evolution of a depression ISW across two triangular obstacles using numerical modelling and laboratory experiments. The experimental results confirm that the intervals and relative height between the two obstacles are important factors in the interaction of an ISW with the obstacles. However, in the case of the movement of an ISW of depression-type across the Luzon Strait, the effect of the two ridges on the characteristics of the ISW might be less significant than that from the shelf-slope, due to the variations in relative water depth. Results from numerical experiments also show that the amplitude of an ISW can be augmented once the wave commences its contact with a shelf-slope, where an internal hydraulic jump and wave breaking with vortex motion are evident in the laboratory experiments. Eventually, an ISW of depression-type could become an elevation-type at the edge of the continental shelf landwards beyond the turning point, where the upper layer is larger than the bottom layer in a stratified water column.     

Studies on mercuric sulfide,a component of cinnabar,a Chinese herbal medicine,on celluar functions in mouse lung and fibroblasts

Although the cytotoxic effects of mercuric chloride (HgCl₂) and methylmercury chloride (MeHg) have been extensively studied, the insoluble mercuric sulfide (HgS) has been the subject of fewer studies. Since the traditional Chinese mineral drug, cinnabar (containing >95% HgS) continues to be used as an ingredient for infant sedation, the pharmacological and toxicological effects of HgS need to be clarified. In previous experiments, HgS and cinnabar were shown to be absorbed from the gastrointestinal tract (GIT) and distributed in various tissues including the lungs. Thus, a preliminarily examination of whether HgS might exert any oxidative stress on a mouse lung was undertaken. HgS reduced GSH content and increased lipid peroxidation in the lung. Further studies on the cytotoxic effects and the possible mechanisms of action of HgS were compared with HgCl₂ and MeHg in cultured lung fibroblast V₇₉ cells. The results showed that HgS produced cytotoxicity at a concentration (400–1200 µM)in a dependent manner with IC₅₀ of 795.6 µM, as compared to HgCl₂ and MeHg, 8.1 µM and 5.9 µM, respectively. In addition, the HgS induced the phenomena of DNA fragmentation, increasing reactive oxygen species (ROS) and decreasing mitochondrial membrane potential, accompanied by decreased levels of intracellular ATP and GSH and higher lipid peroxidation levels, similar to HgCl₂ and MeHg, but with different toxicokinetic properties. These findings provide evidence for understanding the mechanisms underlying the toxic effects of HgS.     

Assessing carbon footprint in the life cycle of accommodation services: the case of an international tourist hotel

The accommodation sector is the most demanding energy consumer among all building stock categories. This study quantifies an international tourist hotel in Taiwan to holistically estimate the greenhouse gas emissions of hotel accommodation services through a complete life cycle inventory. The study also investigates the difference between the carbon emissions of the baseline and reduction years to understand the effects of carbon emission reduction on the hotel industry. Results show that energy consumption is the main source of carbon footprint. However, the carbon emissions of other activities, such as the production and transportation of hotel amenities and laundry services, are 15.90% for the baseline year and 16.03% for the reduction year. These values are larger than the 5% cutoff rule according to PAS 2050. Thus, these factors should also be considered in assessing the carbon emissions of accommodation services. Hotel occupancy rate significantly affects carbon emissions during a one-night stay in a standard room as the functional unit. The selection of the functional unit should be considered in the hotel strategy. Although this case study involves an international tourist hotel in Taiwan, the findings and recommendations for improvements can be applied elsewhere.     

Recycling rates of waste home appliances in Taiwan

The objective of this study is to provide a calculation of recycling rates of waste home appliances in Taiwan, for the EPA to amend these rates in order to increase the recycling efficiency. The recycling rate is calculated by a formula according to the statistical results obtained through: (1) an estimation of domestic use of home appliances using time series analysis with multiplicative seasonal ARIMA model, (2) a further estimation of generated waste home appliances based upon the estimated domestic use and the corresponding distribution of lifetime span, and (3) a cost analysis of recycling home appliances based on a sampling survey with stratified systematic sampling conducted among collectors and a survey on five recycling plants of waste home appliances. The suggested recycling rates in this study finally used by the EPA show that all of the recycling rates have increased compared with the rates in previous years. This study also implies that amending the recycling rates may only solve some recycling problems temporarily; however, the recycling system of waste home appliances in Taiwan has to be reformed to increase the recycling efficiency for the long term.     

The Reuse of Urban and Industrial Waste in Tai-Lin-Pu Reclamation Project, Taiwan

This study introduces the principles of KMG's (Kaoshiung Municipal Government) dealing with the non-poisonous urban and industrial waste through reclamation of shore land in reinforcing a sense of coastal protection and land development in Tai-Lin-Pu coastal area, southern Taiwan.

Through a series of experimental studies, we found that substitutes of coarse aggregate with a broad spectrum of integrating slag powder, fly ash, and cementitious material can be obtained with a benefit up to 80% saving of cement. the integrated aggregates from the non-poisonous industrial wastes were subsequently made into armour units and used in the field tests at Tai-Lin-Pu coastal area, where the shorelines are seriously eroded. After being subjected to several severe typhoon advents, the results showed that the waste-made units used as the protection breakwater, together with construction wastes and excavated soil as the filling material, prove to be an effective practice in utilizing recycled urban waste to reclaim erosive shore lands. Moreover, this study also demonstrates that through detailed analysis of the waste characteristics, scrap material could be turned into valuable construction aggregates, and highlights the value of non-poisonous urban and industrial waste as a alternative resource for the shore protection engineering.