植物根瘤内痕量Frankia DNA的提取及鉴定

建立了一种简捷、快速植物根瘤内痕量Frankia DNA的提取方法,即采集自然态下根瘤,剥离瘤瓣,取瘤瓣尖经液氮研主民粉末,以20g/L CTAB（十六烷基三甲基溴化铵）消化胞壁,然后以d=5-10μm的石英粉吸附DNA,再以无菌去离子蒸馏水释放DNA,所获DNA产率及纯度较酚／氯仿法好。经对辽宁沙棘、色赤杨根瘤的瘤内痕量Frankia DNA及体外培养的9种Frankia菌株DNA分纯,并用于酶切及PCR,获满意结果。图3参12     

影响马尾松毛虫虫灾发生类型因素的重要性分析

运用列联表分析方法探讨了小班的林地起源、优势树种、地理、生长等因素对小班的马尾松毛虫虫灾发生类型的影响。分析结果表明：小班的海拔高度和坡度是决定小班的马尾松虫灾发生类型的关键因素。小班的优势种结构、平均树高及郁闭度对小班的马尾松毛虫虫灾发生类型也有较大的影响。图12表1参2     

杨树刺槐混交林沙地土壤的水分—物理性质

研究了刺槐与杨树混交林后沙地土壤水分－物理性质变化的状况,结果表明,刺槐与杨树混交后,土壤水分－物理性质得到了改善,表现在土壤最大持水量增加,毛管持水量、田间持水量得以提高,土壤有效持水量也有了较大幅度的提高,刺槐与杨树混交后土壤总孔隙度、非毛管孔隙度、毛管孔隙度得到提高,并由此而改善了混交林土壤的渗透性能,表4参7     

盐生植物种子萌发的特点及其生理基础

用NaCl和等渗PEG处理8种盐生植物和2种非盐生植物种子。结果表明：NaCl和等渗PEG对所有种子的萌发率都产生抑制作用,而且PEG（对盐生植物）的抑制程度大于等渗NaCl,NaCl对盐生植物萌发的抑制水平随处理时间延长而降低,但对非盐生植物种子萌发率的抑制程度增大到8d以后即不再增大。另外,在0．6MPaNaCl下,随处理时间延长,盐地碱蓬和琐琐种子的α－淀粉酶活性不断提高,SOD活性基本不变,但小麦和大麦种子的α－淀粉酶活性在处理8d后接近最大值,SOD活性显著下降,这些结果说明,影响盐生植物种子萌发率的因素与渗透胁迫,种子内部一些关键酶的活性及萌发过程中大量吸收和积累盐离子等都有关系。图6参19     

铝铁锡共聚物的合成及其特性研究

合成制备了铝铁锡共聚物,测定分析了其水解共聚合和熟化过程中的pH变化,并通过扫描电镜和混凝实验对共聚物的晶形貌像和絮凝作用进行了研究。结果表明：Sn^4 具有比Fe^3 ,Al^3 更强的水解活性,它有使共聚物的分子趋于均一的作用;铝铁锡共聚物的最佳配比为：NAl∶NFe∶NSn=5∶5∶3,最佳水解度B^*＝2．0,此共聚物具有比CPAFC更优良的絮凝性能。     

Reproduction in a simultaneous hermaphroditic shrimp, Lysmata wurdemanni: any two will do?

The caridean shrimp Lysmata wurdemanni (Gibbes) displays protandric simultaneous hermaphroditism with out-crossing, but not all males become simultaneous hermaphrodites (euhermaphrodites). In this laboratory study, we attempted to determine why some shrimp remain males. In our experiment, we grew L. wurdemanni from postlarvae to adults in several group sizes and observed their reproductive function. We found that all shrimp reared in isolation become euhermaphrodites. When cultured in a group, the proportion of shrimp remaining male decreased with increasing group size. Except for those that mated within a day, inter-molt euhermaphrodite-phase shrimp (with or without embryos) and inter-molt male-phase shrimp fertilized eggs successfully. On the other hand, euhermaphrodite shrimp can only mate as females and have their eggs fertilized during a narrow post-molt window (less than 12 h) in each molt cycle (10 days). The fertilization rate of male-euhermaphrodite pairs was similar to that of euhermaphrodite-euhermaphrodite pairs. There are at least two non-exclusive explanations for the persistence of male shrimp in a group. In certain group compositions, an individual may gain more reproductive fitness as a large male with multiple mating partners than as a small female with low clutch size. Alternatively, the presence of male-phase individuals, with variable molt-cycle duration (5-8 days), may be necessary to ensure mating. This study is the first direct experimental demonstration of social control of sex change in the decapod crustaceans.     

TEOM法与实验室称重法监测大气中TSP的对比研究

用数理统计的方法计算出TEOM法与称重法监测结果的偏差,用F检验法检验TEOM法与称重法监测结果之间的致性,用r检测法检验TEOM法与称重法监测结果之间的相关性,用t检验法检验TEOM法与称重法监测结果之间的差异,通过对比研究,找出TEOM法与称重法之间的相互关系和规律。     

Efficient microwave degradation of humic acids in water using persulfate and activated carbon

Environmental Chemistry Letters - Humic acids are complex mixtures of organic molecules of different sizes, molecular weights and functional groups such as phenols, carboxyls, quinones and amino...     

Diversity of crude oil-degrading bacteria and alkane hydroxylase (<Emphasis Type="Italic">alkB</Emphasis>) genes from the Qinghai-Tibet Plateau

The aim of this study was to survey the response of the microbial community to crude oil and the diversity of alkane hydroxylase (alkB) genes in soil samples from the Qinghai-Tibet Plateau (QTP). The enrichment cultures and clone libraries were used. Finally, 53 isolates and 94 alkB sequences were obtained from 10 pristine soil samples after enrichment at 10 °C with crude oil as sole carbon source. The isolates fell into the phyla Proteobacteria, Actinobacteria, and Bacteroidetes, with the dominance of Pseudomonas and Acinetobacter. The composition of degraders was different from polar habitats where Acinetobacter sp. is not a predominant responder of alkane degradative microbial communities. Phylogenetic analysis showed that the alkB genes from isolates and enrichment communities formed eight clusters and mainly related with alkB genes of Pseudomonas, Rhodococcus, and Acinetobacter. The alkB gene diversity in the QTP was lower than marine environments and polar soil samples. In particular, a total of 10 isolates exhibiting vigorous growth with crude oil could detect no crude oil degradation-related gene sequences, such as alkB, P450, almA, ndoB, and xylE genes. The Shannon-Wiener index of the alkB clone libraries from the QTP ranged from 1.00 to 2.24 which is similar with polar pristine soil samples but lower than that of contaminated soils. These results indicated that the Pseudomonas, Acinetobacter, and Rhodococcus genera are the candidate for in situ bioremediation, and the environment of QTP may be still relatively uncontaminated by crude oil.     

Fluctuating asymmetry rather than oxidative stress in <Emphasis Type="Italic">Bufo raddei</Emphasis> can be an accurate indicator of environmental pollution induced by heavy metals

Oxidative stress (OS) and fluctuating asymmetry (FA) as risk markers for environmental stress are widely used to predict changes in the health and fitness of many animals exposed to pollutants. However, from the perspective of protecting declining amphibians, it remains to be verified which one would be a reliable indicator for amphibians exposed to long-term heavy metal pollution under natural conditions. In this study, the OS and FA of Bufo raddei exposed to natural heavy metal pollution were analyzed to determine which marker is more accurate for indicating heavy metal-induced stress. Three years of data were collected during the breeding season of B. raddei from Baiyin (BY), which has been mainly contaminated with Cu, Zn, Pb, and Cd compounds for a long period, and from Liujiaxia (LJX), which is a relatively unpolluted area. Unexpectedly, although significant accumulation of the four heavy metals was found in the kidney and liver of B. raddei from BY, the levels of superoxide dismutase, glutathione peroxidase, and malondialdehyde in these two organs were found to be irregular, with low repeatability in both BY and LJX. However, significant differences in the levels of FA were observed in B. raddei populations from these two areas over the past 3 years (P < 0.01). The degrees of FA in B. raddei populations from BY and LJX were assessed as degree 4 and 1, respectively. In short, this study suggested that FA was a more reliable and effective indicator than OS to monitor and predict long-term environmental stress on anuran amphibians.