Tapping practices and SAP yields of the NIPA palm (NIPA Fruticans) in Papua New Guinea

Methods of tapping the nipa palm (Nipa fruticans Wurmb.) were developed in the course of a 3-year study conducted in seven villages in the Baimuru district of the Gulf Province of Papua New Guinea. The experimental sites were located about 1.5–23 km from the sea. Their plant density varied from 4700 to 2400 palms per ha and the mean height of the plants was nearly 13 m.Five pre-treatment patterns were tested. Once-a-week treatment, maintained for 12 weeks, yielded an average of 155 ml of sap/24 h/palm for one month, whereas 3–5-times-a-week treatment, for 5–12 weeks, yielded an average of 1300 ml of sap. The duration of treatment depended on the state of development of the flower stalk. The pre-treatment pattern chosen as the standard was to bend the stalk 12 times in one direction, to pat with the hands the length of the stalk backwards and forwards 64 times, and to give the base four kicks; this was repeated four times a week.In the course of the 3-year study it was found that the average sugar content of fresh nipa sap is rather high, about 16.4% w/v, as compared with, for example, about 12% w/v of sugar cane. The results of the present study are discussed with reference to producing fuel alcohol from the Papua New Guinea nipa palm.     

A chemical view of the most ancient metazoa--biomarker chemotaxonomy of hexactinellid sponges

Hexactinellid sponges are often considered to be the most ancient metazoans. Lipid biomarkers from 23 species were studied for information on their phylogenetic properties, particularly their disputed relation to the two other sponge classes (Demospongiae, Calcarea). The most prominent lipid compounds in the Hexactinellida comprise C28 to C32 polyenoic fatty acids. Their structures parallel the unique patterns found in demosponge membrane fatty acids ('demospongic acids') and strongly support a close phylogenetic association of the Demospongiae and the Hexactinellida. Both taxa also show unusual mid-chain methylated fatty acids (C15-C25) and irregular C25- and C40-isoprenoid hydrocarbons, tracers for specific eubacteria and Archaea, respectively. These biomarkers indicate a similar, highly conservative symbiont community, although some shift in the abundance of the associated microbiota was observed. The lack of these features in calcareous sponges further contradicts the still common view that Calcarea and Demospongiae are more closely related to each other than either is to the Hexactinellida.     

False-negative prenatal exclusion of Wiskott-Aldrich syndrome by measurement of fetal platelet count and size

The study of the fetal platelet count and size can, according to the literature, be used for the prenatal diagnosis of the Wiskott-Aldrich syndrome (WAS). So far, no affected fetuses have been identified by this method. All pregnancies in which this method had been applied to resulted, as correctly predicted, in the birth of normal children. Here we report on a familial case of WAS where the haematological parameters failed to reveal the affected second child. Hence we assume that the platelet count and size of platelets remain normal in fetuses with WAS to the gestational age of 22 weeks and cannot be used for prenatal diagnosis.     

Intrauterine mediastinal teratoma associated with non-immune hydrops fetalis

We describe a rare case of non-immune hydrops fetalis caused by mediastinal teratoma. The sonographic appearance was that of a mixed cystic and solid mass in the antero-superior mediastinum. The teratoma, on post mortem, extended cranially to the upper part of the thyroid, exerting pressure and causing deviation of the trachea, oesophagus, and aortic arch. The pathogenesis of non-immune hydrops fetalis suggests obstruction of venous return caused by this tumour.