Profiling of environmental Legionella pneumophila strains by randomly amplified polymorphic DNA method isolated from geographically nearby buildings

Legionella pneumophila (L. pneumophila) which is also known as etiologic agent Legionnaires Disease lives in natural water and man made water systems. These bacteria belonging to Legionellaceae family are divided 15 serogroups. Phenotypical methods used for the identification of Legionella isolates are not very discriminatory. In this study we investigated genotypic features of eight L. pneumophila serogroup 1 and 18 L. pneumophila serogroup 2-14 strains isolated from different buildings in Istanbul by randomly amplified polymorphic DNA (RAPD) method. Eight L. pneumophila serogroup 1 strains (37.5%) were similar RAPD profile and they were isolated from buildings located in a short distance (about 500 m). Four L. pneumophila serogroup 2-14 strains (22%) were identical genotypically. Three of these strains were isolated from buildings located in a short distance.     

Metal Concentrations in Fish Species from the Northeast Mediterranean Sea

Samples of Mugil cephalus and Mullus barbatus were collected in the Northeast Mediterranean coast of Turkey the contents of cadmium, copper, iron, zinc and lead in the liver, gill and muscle tissues were determined by atomic absorption flame spectrophotometry.Except for lead, highest levels of each metal were found in the liver and this was followed by the gill and muscle in both species. Among the metals analyzed, Cu, Zn and Fe were the most abundant in the different tissues while Cd and Pb were the least abundant both in M. cephalus and M. barbatus. Seasonal changes in metal (Cd, Cu, Pb, Fe and Zn) concentration were observed in the tissues of both species, but these seasonal variations may not influence consumption advisories. In general, the highest concentrations were detected for all metals in summer.     

Seasonal variation of some heavy metal pollution with environmental and microbiological parameters in sub-basin of Kocabas Stream (Biga, Canakkale, Turkey) by ICP-AES

Waste water pollution in industrial areas is one of the most important environmental problems. Heavy metal pollution, especially chromium species in waste water sources from tannery affects our lives. Kocabas Stream is located in south-west Marmara region and Biga town is positioned in the sub basin on the stream. This water source functions as the water for irrigation in agriculture, drinking water for animals and for human use. Thus, this study is of great importance. Waste water pollution can affect all ecosystems and human health by directly or indirectly as in food chain. The concentration of heavy metals (Pb, Cd, Cu, Zn and Cr) were pre-analysed by ICP-AES method in water samples taken from sub-basin of Kocabas stream. In the results of these analyses, concentrations of the metals except chromium were founded at the limit value. But the total concentration of the Cr was found at high levels of between 0.0082 +/- 0.0001 and 5.7231 +/- 0.0921 mg l(-1) over the limit value (0.05 mg l(-1); WHO, EPA, TSE 266 and inland water quality classification) at sampling points very close to tannery factories. Also physicochemical and microbiological parameters of Kocabas Stream were determined. The effects of the experimental results on environment were investigated.     

Stereological quantification of placental villus vascularization and its relation to umbilical artery Doppler flow in intrauterine growth restriction

The aim of this study was to investigate the changes in the number of vessels within tertiary stem villi and intermediate/terminal villi which may be responsible for the abnormalities in placental vascular resistance and Doppler velocity index values in growth-restricted fetuses. The placentas of 20 cases with intrauterine growth restriction and 30 cases which were appropriate for gestational age were studied. The umbilical artery resistance index, pulsatility index and systolic to diastolic ratio were measured in each case. The vessels were quantified by a stereological method described previously and vascular surface density and the volume portion of the villi were calculated. The placentas of preterm and term cases with intrauterine growth restriction displayed significant reductions in the vascular surface density of stem and intermediate/terminal villi and volume portion of intermediate/terminal villi stroma when compared with gestation-matched normally grown cases (p<0.05). There was no significant correlation between Doppler index values of the umbilical artery and the stereological parameters of the intermediate/terminal and stem villi in the intrauterine growth restriction group (p>0.05). Some of the pregnancies with intrauterine growth restriction (six patients) with normal Doppler flow velocity waveforms had reduced vascularization in the placentas, and these pregnancies were found to have no perinatal complications. We conclude that,although the placental villi show reduced vascularization in pregnancies with intrauterine growth restriction, the Doppler indices may be normal and this normal flow pattern is related to reduced complication rate. Copyright © 2002 John Wiley & Sons, Ltd.     

Stack gas dispersion measurements with Large Scale-PIV,Aspiration Probes and Light Scattering Techniques and comparison with CFD

The main purpose of this research is to manage simultaneous measurement of velocity and concentration in large cross-sections by recording and processing images of cloud structures to provide more detailed information for e.g. validation of CFD simulations. Dispersion from an isolated stack in an Atmospheric Boundary Layer (ABL) was chosen as the test case and investigated both experimentally and numerically in a wind tunnel. Large Scale-Particle Image Velocimetry (LS-PIV), which records cloud structures instead of individual particles, was used to obtain the velocity field in a vertical plane. The concentration field was determined by two methods: Aspiration Probe (AP) measurements and Light Scattering Technique (LST). In the latter approach, the same set of images used in the LS-PIV was employed. The test case was also simulated using the CFD solver FLUENT 6.3. Comparison between AP measurements and CFD revealed that there is good agreement when using a turbulent Schmidt number of 0.4. For the LST measurements, a non-linear relation between concentration and light intensity was observed and a hyperbolic-based function is proposed as correction function. After applying this correction function, a close agreement between CFD and LST measurements is obtained.     

Using landsat data to determine land use/land cover changes in Samsun, Turkey

The rapid industrialization and urbanization of an area require quick preparation of actual land use/land cover (LU/LC) maps in order to detect and avoid overuse and damage of the landscape beyond sustainable development limits. Remote sensing technology fits well for long-term monitoring and assessment of such effects. The aim of this study was to analyze LU/LC changes between 1980 and 1999 in Samsun, Turkey, using satellite images. Three Landsat images from 1980, 1987 and 1999 were used to determine changes. A post classification technique was used based on a hybrid classification approach (unsupervised and supervised). Images were classified into six LU/LC types; urban, agriculture, dense forest, open forest-hazelnut, barren land and water area. It is found that significant changes in land cover occurred over the study period. The results showed an increase in urban, open forest/hazelnut, barren land and water area and a decrease in agriculture and dense forest in between 1980 and 1999. In this period, urban land increased from 0.77% to 2.47% of the total area, primarily due to conversions from agricultural land and forest to a lesser degree. While the area of dense forest decreased from 41.09% to 29.64% of the total area, the area of open forest and hazelnut increased from 6.73% to 11.88%.     

珠海码头内铜污染现状及生态风险分析

以珠海码头作为调查船舶防污漆使用影响的对象,分析了珠海周边海域码头内表层沉积物与海水中的重金属含量,以Cu元素为讨论重点,对其进行污染程度与潜在生态危害的评估。结果表明,码头内表层沉积物中Cu含量明显高于码头外沉积物,离市区较近的香洲码头和金沙滩码头内沉积物受到中等至较高程度的Cu污染,海岛东澳岛码头海水易流通,因此受污染程度较低。使用Hakanson生态危害指数法对沉积物进行评估的结果为低潜在生态危害,但码头内海水中Cu元素含量已高于生态安全限值。     

Salmonella mutagenicity analysis of water samples from Çamaltı saltern

Mutagenicity analysis of water samples from Camalti Saltern, Turkey were investigated by applying Salmonella mutagenicity test. Plate incorporation assay was applied in the absence of metabolic activation. XAD 4 and XAD 16 columns were used for the fractionation of the water samples. The results obtained were negative in both TA 98 and TA 100. Although the number of revertants of XAD 16 extract in station B were higher than the revertants in the other stations for TA 98 strain when compared to the solvent control. However this was not significant to be considered as mutagenic. The results were discussed for the future analysis.     

Distributions of 210Pb around a uraniferous coal-fired power plant in Western Turkey

In the present study the spatial and the vertical distributions of 210Pb were investigated in the soils around a uranifereous coal fired power plant (CPP) in Yatagan Basin, in Western Turkey. The variation of 226Ra activity along the soil profiles was studied to assess the unsupported 210Pb distribution in the same samples. 226Ra was measured by gamma spectroscopy and 210Pb activities were determined from 210Po activities using radiochemical deposition and alpha spectroscopy. The total 210Pb activity concentrations in bulk core samples varied in the range of 38-250 Bq kg(-1) in the study sites and of 22-78 Bq kg(-1) in reference site. In the sectioned cores sampled from the study areas the ranges for activity concentrations of 226Ra, total 210Pb and unsupported 210Pb are 24-77; 39-344 and 4-313 Bq kg(-1), respectively. Corresponding ranges for reference site are 37-39; 39-122 and 1-83 Bq kg(-1).