Applying adult emergence as an endpoint in a post-exposure laboratory test using two midge species (Diptera: Chironomidae)

Several approaches have been used to evaluate biological impairment in aquatic ecosystems which can be categorized as either laboratory or field. In the recent years, the laboratory toxicity test approach has been extended to field exposures where ambient factors are allowed to influence the test response. Field exposures of laboratory test organisms require method modifications. In this paper, a novel in situ method is described which measures growth, survival and emergence of sediment inhabiting insects (Diptera: Chironomidae) that are used in standardized laboratory toxicity testing. Two standard chironomid species (Chironomus riparius and Chironomus tentans) were used to test the suitability of the approach and to compare the performance of the species. The larvae were transferred to the laboratory for emergence after 7 days in situ exposure which was compared to laboratory responses. Growth, survival and emergence were significantly lower in the in situ pre-exposure than in the laboratory. Also, emergence success was significantly lower in one reference sediment (LMR) than in the other test sediments in both in situ and the laboratory treatment. These lower response levels likely resulted from sediment characteristics and artifacts related to the exposure in the in situ chamber. Feeding and water quality within the exposure chamber appear to be factors that may differ markedly from the laboratory exposure and may affect organism responses. C. riparius developed (growth, emergence time) faster than C. tentans in all treatments, otherwise the species responded similarly. C. riparius may be a better alternative for the chronic in situ exposures because of shorter exposure times and reduced feeding requirements.     

Predation, habitat complexity, and variation in density-dependent mortality of temperate reef fishes

Density dependence in demographic rates can strongly affect the dynamics of populations. However, the mechanisms generating density dependence (e.g., predation) are also dynamic processes and may be influenced by local conditions. Understanding the manner in which local habitat features affect the occurrence and/or strength of density dependence will increase our understanding of population dynamics in heterogeneous environments. In this study I conducted two separate field experiments to investigate how local predator density and habitat complexity affect the occurrence and form of density-dependent mortality of juvenile rockfishes (Sebastes spp.). I also used yearly censuses of rockfish populations on nearshore reefs throughout central California to evaluate mortality of juvenile rockfish at large spatial scales. Manipulations of predators (juvenile bocaccio, S. paucispinus) and prey (kelp, gopher, and black-and-yellow [KGB] rockfish, Sebastes spp.) demonstrated that increasing the density of predators altered their functional response and thus altered patterns of density dependence in mortality of their prey. At low densities of predators, the number of prey consumed per predator was a decelerating function, and mortality of prey was inversely density dependent. However, at high densities of predators, the number of prey killed per predator became an accelerating response, and prey mortality was directly density dependent. Results of field experiments and large-scale surveys both indicated that the strength of density-dependent mortality may also be affected by the structural complexity of the habitat. In small-scale field experiments, increased habitat complexity increased the strength of density-dependent mortality. However, at large scales, increasing complexity resulted in a decrease in the strength of density dependence. I suggest that these differences resulted from scale-dependent changes in the predatory response that generated mortality. Whether increased habitat complexity leads to an increase or a decrease in the strength of density-dependent mortality may depend on how specific predatory responses (e.g., functional or aggregative) are altered by habitat complexity. Overall, the findings of this study suggest that rates of demographic density dependence and the resulting dynamics of local populations may largely depend upon attributes of the local habitat.     

An analysis of effects of San Diego wildfire on ambient air quality

The impact of major gaseous and particulate pollutants emitted by the wildfire of October 2003 on ambient air quality and health of San Diego residents before, during, and after the fire are analyzed using data available from the San Diego County Air Pollution Control District and California Air Resources Board. It was found that fine particulate matter (PM) levels exceeded the federal daily 24-hr average standard during the fire. There was a slight increase in some of the gaseous pollutants, such as carbon monoxide, which exceeded federal standards. Ozone (O3) precursors, such as total hydrocarbons and methane gases, experienced elevated concentration during the fire. Fortunately, the absence of sunlight because of the cloud of thick smoke that covered most of the county during the fire appears to have prevented the photochemical conversion of the precursor gases to harmful concentrations of O3. Statistical analysis of the compiled medical surveillance data has been used to establish correlations between pollutant levels in the region and the resultant health problems experienced by the county citizens. The study shows that the increased PM concentration above the federal standard resulted in a significant increase in hospital emergency room visits for asthma, respiratory problems, eye irritation, and smoke inhalation. On the basis of the findings, it is recommended that hospitals and emergency medical facilities engage in pre-event planning that would ensure a rapid response to an impact on the healthcare system as a result of a large wildfire and appropriate agencies engage in the use of all available meteorological forecasting resources, including real-time satellite imaging assets, to accurately forecast air quality and assist firefighting efforts.     

Relative risk of abnormal karyotype in fetuses found to have an atrioventricular septal defect (AVSD) on fetal echocardiography

One hundred and twenty-five fetuses were identified as having an AVSD with normal venous connections, normal arterial connections and normal cardiac situs on fetal echocardiography. Fetal karyotype was known in 111 of these cases. The relative risk of fetal trisomy 21 at mid-trimester was 107 (95% CI 87–127) times the expected number of cases compared with risk from maternal age alone, and that for trisomy 21,18 or 13 was 95 (95% CI 79–109). This data may be useful in counselling pregnant women about risk of fetal karyotypic abnormality after a diagnosis of fetal AVSD. Copyright © 2005 John Wiley & Sons, Ltd.     

Open fetal surgery for myelomeningocele

Despite efforts at prevention through the use of preconception folic acid, spina bifida remains one of the most common congenital anomalies of the central nervous system that is compatible with life. It is, however, associated with a significant degree of lifelong morbidity. The development of open fetal surgery for myelomeningocele (MMC) has been a long process but one that serves as a model for how new procedures and technologies need to be properly evaluated before being brought into mainstream medical practice. Even so, risks and benefits need to be evaluated for each patient. The currently available studies have been carried out on a highly selected patient population where the fetal findings provided the maximum opportunity for benefit from prenatal closure of the MMC defect. There is the potential that as the surgery becomes more widely available, pressure will be brought to bear to perform surgery in cases where the likelihood for benefit is decreased and yet the risks are not. The only way to duplicate the results of the current studies is to follow the methodology and criteria that were used in the studies. This will mean that not every fetus with an MMC will be a candidate for in utero surgery. The balance of risk to benefit will continue to evolve as further technological advances are evaluated and more follow-up information is obtained. Copyright © 2011 John Wiley & Sons, Ltd.     

Temporal variation of recruits as a basis of ephemeral genetic heterogeneity in the western rock lobster Panulirus cygnus

Allozymes were examined in quantitative lunar monthly collections of larval recruits of the western rock lobster Panulirus cygnus George over three recruitment seasons at two sites nearly 350 km apart in Western Australia. At Alkimos, the southern site, recruitment occurs in a relatively narrow peak early in the spring, whereas at the northern Houtman Abrolhos Islands, recruitment extends into the summer months. In the 1995/1996 recruitment season, the frequency of the GPI ^* 100 allele increased from early to late in the season, but the frequencies were indistinguishable at the two sites in each monthly collection. The combination of this temporal variation in allelic frequencies with the contrasting patterns of recruitment at the Abrolhos Islands and Alkimos resulted in genetically different cohorts at the two sites. This pattern was ephemeral, as it was not repeated in the subsequent two years. Thus, ephemeral genetic patchiness in P. cygnus can be generated by the locally-specific genetic mix of recruits obtained from a common larval pool. This mechanism is the probable explanation of previously observed temporal and possible spatial genetic variation in adult P. cygnus, and highlights the importance of studying recruitment in order to understand the genetic structure of marine species. Received: 22 February 1999 / Accepted: 8 June 1999     

Microtox assay to determine the toxicity of degradation products from degradable plastics

Six types of starch-polyethylene degradable plastics were evaluated for the release of water-soluble toxic compounds under accelerated degradation conditions. A plastic strip (2.5×15.2 cm) was placed in a 250-ml Erlenmeyer flask with 100 ml of ASTM type I water with or without trace element solutions and shaken at 65°C and 110 rpm for 20 weeks in replicates of two. High temperature was used to accelerate the oxidative degradation of polyethylene. Plastic degradation was measured by loss of tensile strength, percentage elongation, strain energy, and weight-average molecular weight. The most rapid period of polyethylene thermal degradation was complete for most materials by day 28. Ten-milliliter aqueous samples were removed from each flask at days 1, 7, 28, 56, 84, and 140 (water volumes were maintained at 100 ml with fresh type I water), filtered through glass filters, then evaluated by using the Microtox Toxicity Analyzer (Microbics Corporation, Carlsbad, CA). No water-soluble toxic compounds were detected during the period of rapid film degradation. Toxicity was observed at day 28 for one film and at day 84 for all films, which could possibly correlate with the release of small oxidative compounds such as formaldehyde and acetaldehyde. Because of the sensitivity of this assay, positive results must be confirmed by otherin vitro studies.Journal Paper No. J-14851 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa. Projects No. 2889 and 0178.     

ENVIRONMENTAL IMPACT OF STREAM CHANNELIZATION1

ABSTRACT Geologic, engineering, and biological investigations of six Pennsylvania coldwater streams were undertaken to determine the impact of channel modifications instituted both prior to and following Hurricane Agnes. The primary focus of the study was on the ecological changes brought about by stream channelization. No long-term deleterious effects on water quality, attached algae, benthic fauna, or forage fish populations were found. Trout, however, were found to be greater in numbers and weight in natural than in channelized stream reaches. Lack of suitable physical habitat appears to be the primary cause of reduced trout populations in stream reaches which have been channelized.