Genetic sex determination in <Emphasis Type="Italic">Astatotilapia calliptera</Emphasis>, a prototype species for the Lake Malawi cichlid radiation

East African cichlids display extensive variation in sex determination systems. The species Astatotilapia calliptera is one of the few cichlids that reside both in Lake Malawi and in surrounding waterways. A. calliptera is of interest in evolutionary studies as a putative immediate outgroup species for the Lake Malawi species flock and possibly as a prototype ancestor-like species for the radiation. Here, we use linkage mapping to test association of sex in A. calliptera with loci that have been previously associated with genetic sex determination in East African cichlid species. We identify a male heterogametic XY system segregating at linkage group (LG) 7 in an A. calliptera line that originated from Lake Malawi, at a locus previously shown to act as an XY sex determination system in multiple species of Lake Malawi cichlids. Significant association of genetic markers and sex produce a broad genetic interval of approximately 26 megabases (Mb) using the Nile tilapia genome to orient markers; however, we note that the marker with the strongest association with sex is near a gene that acts as a master sex determiner in other fish species. We demonstrate that alleles of the marker are perfectly associated with sex in Metriaclima mbenjii, a species from the rock-dwelling clade of Lake Malawi. While we do not rule out the possibility of other sex determination loci in A. calliptera, this study provides a foundation for fine mapping of the cichlid sex determination gene on LG7 and evolutionary context regarding the origin and persistence of the LG7 XY across diverse, rapidly evolving lineages.     

The evolution of Müllerian mimicry

It is now 130 years since Fritz Müller proposed an evolutionary explanation for the close similarity of co-existing unpalatable prey species, a phenomenon now known as Müllerian mimicry. Müller's hypothesis was that unpalatable species evolve a similar appearance to reduce the mortality involved in training predators to avoid them, and he backed up his arguments with a mathematical model in which predators attack a fixed number (n) of each distinct unpalatable type in a given season before avoiding them. Here, I review what has since been discovered about Müllerian mimicry and consider in particular its relationship to other forms of mimicry. Müller's specific model of associative learning involving a "fixed n" in a given season has not been supported, and several experiments now suggest that two distinct unpalatable prey types may be just as easy to learn to avoid as one. Nevertheless, Müller's general insight that novel unpalatable forms have higher mortality than common unpalatable forms as a result of predation has been well supported by field experiments. From its inception, there has been a heated debate over the nature of the relationship between Müllerian co-mimics that differ in their level of defence. There is now a growing awareness that this relationship can be mediated by many factors, including synergistic effects between co-mimics that differ in their mode of defence, rates of generalisation among warning signals and concomitant changes in prey density as mimicry evolves. I highlight areas for future enquiry, including the possibility of Müllerian mimicry systems based on profitability rather than unprofitability and the co-evolution of defence.     

Assessment of inherent vulnerability of forests at landscape level: a case study from Western Ghats in India

Assessment of vulnerability is an important step in building long-term resilience in the forestry sector. The objective of this paper is to present a methodological approach to assess inherent vulnerability of forests at landscape level. The approach involves use of vulnerability indicators, the pairwise comparison method, and geographic information system (GIS) tools. We apply this approach to assess the inherent vulnerability of forests of the Western Ghats Karnataka (WGK) landscape, which is a part of the Western Ghats biodiversity hotspot in India. Four vulnerability indicators, namely biological richness, disturbance index, canopy cover, and slope, are selected. We find that forests in 30, 36, 19, and 15 % grid points in this region show low, medium, high, and very high inherent vulnerability, respectively. The forest showing high and very high inherent vulnerability are mostly dry deciduous forests and plantations located largely on the eastern side of the landscape. We also find that canopy cover is one of the key indicators that determine the inherent vulnerability of forests, and natural forests are inherently less vulnerable than man-made plantations. Spatial assessment of inherent vulnerability of forests at landscape level is particularly useful for developing strategies to build resilience to current stressors and climate change in future.     

Linking the EU emissions trading scheme: economic implications of allowance allocation and global carbon constraints

We investigate the role of domestic allowance allocation and global emissions constraints for the carbon-market impacts of linking the EU Emissions Trading Scheme (ETS) internationally. Employing a quantitative simulation model of the global carbon market, we find that the economic benefits from connecting the European ETS to emerging non-EU schemes strongly depend on the regional allowance allocation of the linking participants: In a world of moderate carbon constraints, an economically efficient regional allowance allocation induces a much stronger fall in total compliance costs than a sub-optimal (i.e. too high) domestic allocation of emissions permits. However, a more efficient (i.e. stricter) allocation shifts abatement efforts and compliance costs to energy-intensive industries which are covered by the domestic ETS. We further find that committing to ambitious global emissions reduction targets (compatible with stabilizing CO₂ concentrations at 450 ppm) induces much stronger regional abatement efforts and substantially higher compliance costs for the abating regions. In such an ambitious climate policy regime, an efficient domestic allocation of allowances is even more important from an economic perspective: Here, linking emissions trading schemes diminishes the associated compliance costs on the largest scale.

Establishment of facultative sexuals

The existence of sex is one of the major unsolved problems in biology. We use computer simulations to model conditions in which sex may first become established. We develop an individual-based population model and show that a hypothetical facultative sex gene can fix, provided that the initial cost is low. It is demonstrated that the equilibrium fitness in the population increases with increasing population size and decreasing mutation rate. The probability of the establishment of the sex gene is found not to be directly related to the fitness difference between the asexual and sexual populations. This change in fitness on changing the parameters of the model is investigated.     

Dimensional analysis and scaling in mechanical mixing for fabrication of metal matrix nanocomposites

For a successful enhancement of mechanical properties of metal matrix nanocomposites, a homogeneous nanoparticle dispersion and distribution in the solidified metal is required. Mechanical mixing can be used for initial break-up of agglomerates, and its study can be simplified with dimensional analysis. Using this technique, mixing time and vortex height were assessed while varying fluid properties, impeller angle, and angular speed. Three relevant dimensionless numbers were recognized: the Reynolds (Re), Froude and Galilei (Ga) numbers. Based on blade and impeller shaft angles, a modified Froude number (Fr*) was defined. These parameters were calculated experimentally, varying angular speed from 200 to 1000 rpm for three different impeller angles: 0°, 15° and 30°. This procedure was performed with three fluids: water, and two aqueous glycerin solutions (25% and 50% by volume). Digital images were taken and processed to measure vortex height. Mixing time was measured for water at 0° impeller angle, angular speed ranging from 200 to 1200 rpm. Results showed an optimal dimensionless mixing time with respect to Re. A linear relationship was found between dimensionless vortex height and Fr*. The first had a second order polynomial relationship with the product ReFr*, regardless of impeller angle. This relationship, together with the Ga, specific for each fluid, allows scaling the results to other fluids such as molten pure aluminum. This study allows experimenting in simpler systems that involve transparent fluids, room temperature and low cost, to then elaborate a prediction of vortex height in fluids where measurements are difficult and costly, such as molten metals.     

活性氮和世界:200年的变化

本文通过将19世纪后期和20世纪后期的氮流动情况进行对比,分析了食物和能源生产对全球氮循环的影响.我们虽然对人类产生的活性氮的数量及其释放到环境所产生的主要影响有了充分的认识,但是,我们对于环境库中氮的累积速度缺乏了解,由于积累在环境中的氮具有阶式效应,因此,对其在环境中的积累尚有不明之处.未来活性氮的产生、分布状况及其形成速度增加的可能性(尤其是在亚洲)存在巨大的区域性差异,因而需要制定"总活性氮措施",以优化食物和能源生产,保护环境系统.     

Transport company safety climate—The impact on truck driver behavior and crash involvement

Objective: The present study investigated the relationships between safety climate and driving behavior and crash involvement.

Methods: A total of 339 company-employed truck drivers completed a questionnaire that measured their perceptions of safety climate, crash record, speed choice, and aberrant driving behaviors (errors, lapses, and violations).

Results: Although there was no direct relationship between the drivers' perceptions of safety climate and crash involvement, safety climate was a significant predictor of engagement in risky driving behaviors, which were in turn predictive of crash involvement.

Conclusions: This research shows that safety climate may offer an important starting point for interventions aimed at reducing risky driving behavior and thus fewer vehicle collisions.