芹菜素对丙烯腈引起大鼠精子脂质过氧化和DNA损伤的影响

分析芹菜素(apigenin,AP)对丙烯腈(acrylonitrile,ACN)引起的大鼠精子脂质过氧化和DNA损伤的影响,并探讨其可能的机制。将50只SPF级SD成年雄性大鼠随机分为阴性对照组(玉米油)、ACN组(50 mg·kg~(-1)ACN)、低AP组(50 mg·kg~(-1)ACN+234 mg·kg~(-1)AP)、高AP组(50 mg·kg~(-1)ACN+468 mg·kg~(-1)AP)、N-乙酰半胱氨酸(N-acetylcysteine,NAC)组(50 mg·kg~(-1)ACN+300mg·kg~(-1)NAC),以5 m L·(kg bw)~(-1)灌胃染毒,1次·d~(-1),6 d·周~(-1),连续13周。检测大鼠精子活性氧(ROS)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及精子DNA损伤情况。结果发现,ACN组、低AP组、高AP组、NAC组精子ROS、MDA含量显著升高,SOD活力显著降低,精子尾部DNA含量百分比、尾长、尾距、Olive尾距均显著增高于对照组(均P0.05);而低AP组、高AP组、NAC组精子ROS、MDA含量、SOD活性和精子DNA损伤情况与ACN组相比差异均无统计学意义(P0.05)。提示ACN可引起大鼠精子脂质过氧化和DNA损伤,而AP、NAC对其无干预作用。     

超高压工程电缆绝缘油对海洋生物的生态毒性效应

随着超高压联网工程建设项目不断增多,取得巨大经济效益的同时,其电缆安全性日益受到关注。采用标准实验方法,研究电缆绝缘油对8种占据不同生态位的海洋生物(费氏弧菌(Vibrio fischeri)、牟氏角毛藻(Chaetoceros mueleri)、卤虫(Artemia sp.)、蒙古裸腹溞(Moina mongolica)、凡纳滨对虾(Litopenaeus vannamei)、裸项栉鰕虎鱼(Ctenogobius gymnauchen)、双齿围沙蚕(Perinereis aibuhitensis)、菲律宾蛤仔(Ruditapes philippinarum))的急性毒性,同时进行了电缆绝缘油对裸项栉鰕虎鱼14 d延长毒性实验和电缆绝缘油对蒙古裸腹溞的慢性毒性。结果表明,电缆绝缘油在50%饱和溶解浓度下未对费氏弧菌产生明显的发光抑制作用;对牟氏角毛藻、凡纳滨对虾、裸项栉鰕虎鱼、双齿围沙蚕和菲律宾蛤仔等5种生物未表现出急性毒性影响,其LC50均大于饱和浓度;对卤虫的96 h-LC50为17.07%,LOEC为12.5%,NOEC为6.25%;对蒙古裸腹溞96 h-LC50为29.75%,LOEC为25.0%,NOEC为12.5%;电缆绝缘油对卤虫和蒙古裸腹溞有剧毒。对裸项栉鰕虎鱼成鱼14 d延长毒性LC50大于500 000 mg·L~(-1);对蒙古裸腹溞母溞存活数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1);对母溞存活期的NOEC为312μg·L~(-1),LOEC为625μg·L~(-1);对产胎数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1);对产幼溞数的NOEC为625μg·L~(-1),LOEC为1 250μg·L~(-1)。     

Fenton法和类Fenton法降解土壤中的二苯砷酸

本文对Fenton法与类Fenton法降解土壤中的二苯砷酸(diphenylarsinic acid,DPAA)进行了研究.考察了H2O2投加量和催化剂种类(Fe2+/Fe3+)对红壤及黑土中DPAA降解效果的影响,并采用高效液相色谱-质谱联用法(HPLC-MS/MS)对降解中间产物进行了初步鉴定.结果显示,针对红壤与黑土分别采用类Fenton法与Fenton法,在H2O2投加浓度为1 mol·L-1,含铁催化剂浓度为0.25 mol·L-1,土水比为1∶3,反应时间为1h的条件下,红壤及黑土中DPAA的降解率均可达到65%以上.HPLC-MS/MS的分析结果表明,DPAA可脱苯环形成降解产物苯砷酸(phenylarsinic acid,PAA),而PAA进一步氧化生成无机砷,这可能是Fenton/类Fenton法降解DPAA的途径之一.     

微孔曝气与覆盖对城市重污染河道底泥磷形态分布及释放过程的影响

采用自行研发的泥-水界面微孔曝气系统,开展了底泥表面曝气和覆盖对城市重污染河道底泥磷释放及形态分布规律的影响研究.结果表明,微孔曝气能够有效提高上覆水的溶解氧(DO)和沉积物的氧化还原电位(Eh),能够将泥-水界面Eh维持在-100 m V左右,DO提高到6 mg·L-1以上.与对照比较,原位覆盖处理的上覆水DO和Eh有一定提高,但仍明显低于微孔曝气处理.与对照相比较,微孔曝气处理均有效降低上覆水中总磷(TP)和溶解性正磷酸盐(PO3-4)的含量.试验结束时,微孔曝气(A)和微孔曝气+原位覆盖处理(A+C)上覆水中TP含量由初始的0.201 mg·L-1分别降至0.062 mg·L-1和0.050 mg·L-1;上覆水中PO3-4含量由0.086 mg·L-1和0.078 mg·L-1分别降至0.026 mg·L-1和0.023 mg·L-1.与对照相比,微孔曝气处理明显降低了底泥间隙水中TP的浓度,在整个培养期间,其TP含量平均下降38.8%(A)和47.9%(A+C).底泥原位覆盖处理对抑制泥-水界面磷释放能力要弱于微孔曝气处理,而且在试验后期(50 d),上覆水中TP和PO3-4的含量均有所反弹.不管有无覆盖,泥-水界面微孔曝气处理均显著改变了表层底泥磷形态分布特征,显著降低了底泥中铁铝结合态磷(Fe/Al-P)组分比例,而钙结合态磷(Ca-P)含量比例却出现明显增加.单一的表面覆盖处理对底泥磷形态分布特征没有显著影响(P0.05).研究表明,与单一的处理效果相比较,泥-水界面纳米微孔曝气处理,并结合底泥原位覆盖,更有利于抑制城市重污染河道泥-水界面中磷的释放风险.     

Fecal DDA as a biomarker of DDT exposure in chickens

Despite the largely theoretical risks for human morbidity from exposure to DDT (p, p′-dichlorodiphenyltrichloroethane), the reality that it is inexpensive and highly effective in reducing morbidity and mortality from malaria vectors has encouraged its continued use. Here we present data indicating that domestic fowl are potentially excellent sentinel species for detecting possible human exposures to DDT. In addition to measuring residues of DDT in chicken blood or eggs, a potential alternate analyte indicative of recent DDT exposure is the definitive metabolic product DDA (2,2-bis(4-chlorophenyl)acetic acid) in feces.     

Effects of initial decomposing leaf litter of Cinnamomum camphora on the growth and physiology of Impatiens balsamina北大核心CSCD

A pot experiment was conducted to study the allelopathic effects of initial decomposing leaf litter of Cinnamomum camphora on growth and physiology of Impatiens balsamina. Three leaf litter treatments included 20, 40 and 80 g of C. camphora leaf litter mixed with 8 kg of soil, namely T1, T2, and T3, respectively. In order to test the effect of leaf litter addition on the permeability and ventilation of soil simultaneously, a parallel trial with steamed leaf litter was conducted with the three treatments of the leaf litter. The leaf litter was steamed for 2 d to remove the secondary metabolites as much as possible, dried, and then mixed with 8 kg of soil, namely Z1, Z2, and Z3, respectively. No leaf litter was added in control (CK). The growth parameters of I. balsamina were determined at the 20 d, 60 d, 100 d and 120 d after sowing and the main physiology indicators were determined at the 60 d. The results indicated that: (1) The ground surface diameter and height of I. balsamina were inhibited significantly at 60 d (P < 0.05). Photosynthetic pigments and gas exchange parameters of I. balsamina were inhibited significantly at 60 d, and the inhibition effect was stronger with increased amount of leaf litter addition. The chlorophyll content, Pn and Ls decreased significantly with increased amount of leaf litter (P < 0.05). The activity of superoxide dismutase (SOD) and peroxidase (POD) in leaves of I. balsamina decreased with the increase of leaf litter addition. The content of MDA in treatments T1, T2 and T3 were all higher than that in CK, which indicated that I. balsamina suffered oxidative damage in a certain degree. The content of free proline (Pro) and soluble sugar (SS) in leaves of I. balsamina decreased significantly with the increase of the leaf litter (P < 0.05), while the content of soluble protein (SP) increased. (2) In the parallel trial, 60 d after sowing, no obvious difference was observed between CK and any steamed leaf litter treatment in terms of the morphological and physiological features stated above (P > 0.05). It indicated that the soil physical properties were not greatly influenced by leaf litter addition in the dose interval designed, or that the release of secondary metabolites from decomposing leaf litter was probably a better reason to explain the inhibition of leaf litter treatment to I. balsamina growth. (3)The compound effect (CE) of leaf litter decomposition on I. balsamina was enhanced with increase of the leaf litter, to 0.169, 0.354, and 0.497, respectively, in treatments of T1, T2 and T3. The study indicated that initial decomposition of C. camphora leaf litter in soil reduces the content of photosynthetic pigments, inhibits photosynthetic capacity and resistance physiology of I. balsamina, weakens its adaptability to the environment, and restrains growth of the plant. © 2015, Science Press. All rights reserved.     

川西高原河流水体CDOM的光化学降解特性

川西高原部分河流溶解性有机碳(DOC)含量极高,因有色溶解性有机质(CDOM)具有独特的光学特性,其光降解特征与规律对于分析高寒区天然水体DOC动态及区域水-陆碳循环具有重要的环境意义.本文选取川西高原两种主要地貌类型的河流共5条,即高山峡谷区河流杂谷脑河、抚边河及岷江,丘状高原区河流白河、黑河,对河流水体CDOM三维荧光光谱(EEMs)与紫外-可见光吸收光谱(UV-Vis)采用平行因子分析(PARAFAC)与二维相关光谱(2D-COS)进行分析.结果表明:①白河和黑河CDOM的5 d降解率分别为64. 85%和63. 43%,光降解速率常数分别为0. 167 d-1和0. 173 d-1;抚边河CDOM浓度低(0. 71 m-1)光降解现象不明显,杂谷脑河和岷江CDOM光降解行为较为复杂;②在光降解过程中,除岷江外其余4条河流荧光溶解性有机质(FDOM)内源特征(FI)逐渐减弱,黑河和白河CDOM芳香性、疏水性特征(SUVA254和SUVA260)及腐殖化程度(HIXa)逐渐降低;③川西高原5条河流中FDOM组分均呈2类4个组分,即C1(275/310 nm,类酪氨酸)、C2 [280(250)/400 nm,UVA类腐殖质]和C3(255/440 nm,UVA类腐殖质)、C4[270(360)/492 nm,UVA类腐殖质],类腐殖质FDOM较易光降解;④5条河流中UVA类腐殖质FDOM(尤其是500 nm发射波段)光降解反应先于类酪氨酸物质;丘状高原河流中UVA类腐殖质FDOM对于光照的敏感度大于高山峡谷区河流;⑤白河的主成分分析中识别出了2个因子,解释了这些参数变化的87. 28%,反映了光降解过程对于CDOM特征、荧光组分等方面的影响.     

生物滴滤池净化二氯甲烷废气的实验研究

在φ50mm生物滴滤池内进行了二氯甲烷废气净化的实验研究.由工厂活性污泥经驯化培养得到菌种,进一步接种、在填料表面挂膜,接种和挂膜约需30d.滴滤池内装填聚丙烯散堆填料,废气和循环液在滴滤池内逆流操作,循环液pH值为7.0±0.5,温度维持在28.5±2℃的条件下,生物膜系统能较好地适应进口浓度的变化.在气体空床停留时间为15.7s、二氯甲烷进口浓度为0.7～3.12g/m³的范围内,去除效率为72.0%～99.1%.滴滤池中的酸性环境对二氯甲烷的降解具有明显抑制作用.     

An integrated assessment method of urban drainage system: A case study in Shenzhen City, China

In recent years, the urban drainage system in China is facing the dual pressure of renovation and construction. This requires that the integrated assessment for the planning and operation of the urban drainage system is obligatory. To evaluate the urban drainage system, an integrated assessment methodology based on the analytic hierarchy process (AHP), integrated simulation, and fuzzy assessment is established. This method is a multi-criteria decision adding app roach to the assessment of the urban drainage system comprehensively. Through the integration of the Storm Water Management Model (SWMM), a simple wastewater treatment plant model, and a surface water quality model, an integrated modelling system for the urban drainage system is developed and applied as a key tool for assessment. Using the established method, a case study in Shenzhen City has been implemented to evaluate and compare two urban drainage system reno vation plans, the distributed plan and the centralized plan. Because of the particularity of this case study, the established method is not applied entirely. Considering the water environ mental impact, ecological impact, technological feasibility, and economic cost, the integrated performance of the distri buted plan is better. As shown in this case study, the proposed method is found to be both effective and practical.     

Review of water pollution control in China

Water resource shortage and pollution has seriously threatened the survival and development of developing countries. Because of China’s specific economical and social circumstances, complete adoption of developed countries’ experience is unrealistic. At present, China needs to develop strategies and technologies in source water pollution control and municipal environmental remediation that embrace the country’s specific need to battle the water resource problem. Among them, efficient source water pretreatment is a critical step to ensure a safe municipal water supply. Unlike developed countries, it is not yet feasible in China to treat water supplied to the household and have it meet the standard of direct drinking; therefore, it is more appropriate to refer to it as service water. As a beneficial supplement, an additional community drinking water network and household drinking water apparatus can be considered.