Interacting pressure and temperature effects on enzymes of marine poikilotherms: Catalytic and regulatory properties of FDPase from deep and shallow-water fishes

At low temperature (3°C), and in the absence of substrate and cofactor, the enzyme, fructose diphosphatase (FDPase), extracted from liver of the benthic fishCoryphaenoides acrolepis, is reversibly inactivated by exposure to relatively low pressures (5,000 psi). At pressures of 20,000 psi at 3°C the native enzyme is irreversibly denatured. When the cofactor and the substrate are present, the enzyme is protected against pressure denaturation; hence, catalysis is insensitive to at least 10,000 psi at 3°C. Similarly, interactions between FDPase and its negative modulator adenosine monophosphate (AMP), are largely pressure insensitive. Pressure sensitivity of the native enzyme and of the catalytic process is much reduced at temperatures above about 9°C. The homologous enzyme from a surface fish,Pimelometopon pulchrum, is strikingly more sensitive to pressure, particularly at low temperatures.     

Effects of copper deficiency and copper toxicity on organogenesis and some physiological and biochemical responses of Scots pine (Pinus sylvestris L.) seedlings grown in hydroculture

The morphological, physiological, and biochemical parameters of 6-week-old seedlings of Scots pine (Pinus sylvestris L.) were studied under deficiency (1.2 nM) and chronic exposure to copper (0.32, 1, 2.5, 5, and 10 μM CuSO₄) in hydroculture. The deposit of copper in the seed allowed the seedlings to develop under copper deficiency without visible disruption of growth. The high sensitivity of Scots pine to the toxic effects of copper was shown, which manifested as a significant inhibition of growth and development. The loss of dominance of the main root and a strong inhibition of lateral root development pointed to a lack of adaptive reorganization of the root system architecture under copper excess. A preferential accumulation of copper in the root and a minor translocation in aerial organs confirmed that Scots pine belongs to a group of plants that exclude copper. Selective impairment in the absorption of manganese was discovered, under both deficiency and excess of copper in the nutrient solution, which was independent of the degree of development of the root system. Following 10 μM CuSO₄ exposure, the absorption of manganese and iron from the nutrient solution was completely suppressed, and the development of seedlings was secured by the stock of these micronutrients in the seed. The absence of signs of oxidative stress in the seedling organs was shown under deficiency and excess of copper, as evidenced by the steady content of malondialdehyde and 4-hydroxyalkenals. Against this background, no changes in total superoxide dismutase activity in the organs of seedlings were revealed, and the increased content of low-molecular-weight antioxidants was observed in the roots under 1 μM and in the needles under 5 μM CuSO₄ exposures.

     

Changes in the species composition and abundance of young-of-the-year fishes in the upper Volga stretch of the Kuibyshev Reservoir during the period of 1991 to 2009

The species composition and abundance of young-of-the-year fishes have been analyzed in catches made in the upper Volga stretch of the Kuibyshev Reservoir between 1991 and 2009. It has been found that the juvenile abundance of roach and blue bream has decreased, while that of perch, silver bream, and spined loach has increased, with young-of-the-year juveniles of common rudd, tubenose goby, round goby, and black-striped pipefish appearing in catches. The results of the study show that, against the background of juvenile population decline in many spring-spawning fish species, the abundance and species diversity of young of the year in summer-spawning species has increased during the observation period.     

Impact of Climate Change on Ecological Quality Indicators and Biogeochemical Fluxes in the Baltic Sea: A Multi-Model Ensemble Study

Multi-model ensemble simulations using three coupled physical-biogeochemical models were performed to calculate the combined impact of projected future climate change and plausible nutrient load changes on biogeochemical cycles in the Baltic Sea. Climate projections for 1961-2099 were combined with four nutrient load scenarios ranging from a pessimistic business-as-usual to a more optimistic case following the Helsinki Commission's (HELCOM) Baltic Sea Action Plan (BSAP). The model results suggest that in a future climate, water quality, characterized by ecological quality indicators like winter nutrient, summer bottom oxygen, and annual mean phytoplankton concentrations as well as annual mean Secchi depth (water transparency), will be deteriorated compared to present conditions. In case of nutrient load reductions required by the BSAP, water quality is only slightly improved. Based on the analysis of biogeochemical fluxes, we find that in warmer and more anoxic waters, internal feedbacks could be reinforced. Increased phosphorus fluxes out of the sediments, reduced denitrification efficiency and increased nitrogen fixation may partly counteract nutrient load abatement strategies.     

Experimental investigation of fast flame propagation in stratified hydrogen–air mixtures in semi-confined flat layers

This paper presents results of an experimental investigation on fast flame propagation and the deflagration-to-detonation transition (DDT) and following detonation propagation in a semi-confined flat layer filled with stratified hydrogen–air mixtures. The experiments were performed in a transparent, rectangular channel open from below. The combustion channel has a width of 0.3 m and a length of 2.5 m. The effective layer thickness in the channel was varied by using different linear hydrogen concentration gradients. The method to create quasi-linear hydrogen concentration gradients that differ in the range and slope is also presented. The ignited mixtures were accelerated quickly to sonic flame speed in the first obstructed part of the channel. The interaction of the fast flame propagation with different obstacle set-ups was studied in the second part of the channel. The experimental results show an initiation of DDT by one additional metal grid in the obstructed semi-confined flat layer. Detonation propagation and failed detonation propagation were observed in obstructed and unobstructed parts of the channel.     

Diclofenac sodium aqueous systems at low concentrations: Interconnection between physicochemical properties and action on hydrobionts

Diclofenac sodium(DS) is a widely used nonsteroidal anti-inflammatory drug(NSAIDs).NSAIDs are poorly removed during standard wastewater treatment.The consequences of the presence of NSAIDs in rivers and lakes at 10~(-11)–10~(-8) mol/L are not yet established;therefore, ecotoxicologists have focused their efforts on studying the effect of lowconcentration NSAIDs on fish and hydrobionts, and also on predicting the potential risks to humans.Literature provides some information about the bioeffects of some NSAID solutions in low concentrations but there is no physicochemical explanation for these phenomena.Studying the physicochemical patterns of DS solutions in the low range of concentrations and establishing an interconnection between the solutions' physicochemical properties and bioeffects can provide a conceptually new and important source of information regarding the unknown effects of DS.The physicochemical properties and action of DS solutions on Ceriodaphnia affinis cladocerans,Paramecium caudatum infusoria, Chlorella vulgaris unicellular green algae, as well as on the growth of the roots of Triticum vulgare wheat seeds, were studied in the calculated concentration range of 1 × 10~(-3)–1 × 10~(-18) mol/L.The relationship between these phenomena was established using the certified procedures for monitoring the toxicity of natural water and wastewater.It was shown for the first time that water solutions of DS are dispersed systems in which the dispersed phase undergoes a rearrangement with dilution, accompanied by changes in its size and properties, which affects the nonmonotonic dependences of the system's physicochemical properties and could cause nonmonotonic changes in action on hydrobionts in the low concentration range.     

Experimental study of flame acceleration and the deflagration-to-detonation transition under conditions of transverse venting

Results of experiments on critical conditions for flame acceleration and the deflagration-to-detonation transition in tubes with transverse venting are presented. Tests were made with hydrogen mixtures in two tubes (inner diameter of 46 and 92 mm) with obstacles. Ratios of vent area to total tube area were 0.2 and 0.4. Venting was shown to influence flame acceleration significantly. The greater the vent ratio, the more reactive the mixture necessary for development of fast flames. Critical conditions for flame acceleration in tubes with venting, expressed through a critical mixture expansion ratio σ_cr, were found to be σ_cr/σ₀1+2, where σ₀ is the critical value for a closed tube. Critical conditions for detonation onset in a vented tube were found to be very close to those in a closed tube with similar configuration of obstacles.     

The "Linh Duong" Pseudonovibos spiralis (Mammalia, Artiodactyla) is a new buffalo

The controversial phylogenetic position of the recently described South-East Asian endemic bovid, Pseudonovibos spiralis, was evaluated on the basis of phylogenetic analyses of originally obtained nearly complete 12S mitochondrial rDNA sequences for this species and Bubalus bubalis and 26 sequences of Bovidae from the Genbank using Cervus elaphus (Cervidae) as outgroup. In most of the phylogenetic analyses performed using PAUP 4.0 (maximum parsimony, maximum likelihood and neighbour-joining), Bovidae consisted of two major clades: Bovinae including the tribes Bovini, Tragelaphini and Boselaphini, and Antilopinae + Caprinae, incorporating all other bovids. In most trees P. spiralis fell within the buffalos (subtribe Bovina) between Bubalus and Syncerus. Therefore, our phylogenetic analyses of bovid mitochondrial 12S rRNA gene sequences suggest the close relationship of this enigmatic species with the buffalos and its placement within the subtribe Bovina.