细胞分裂素基因（T－cyt）在转基因烟草中的表达及其对生长发育的影响

将编码细胞分裂素合成关键酶──异戊烯基转移酶的Ｔ－ｃｙｔ基因分别置于ＣａＭＶ３５Ｓ启动子，ｒｂｃＳ启动子和Ｔ－ｃｙｔ基因自身启动子的调控下，构建成不同的嵌合质粒用于转化烟草，通过ＰＣＲ检测，Ｓｏｕｔｈｅｒｎ杂交和ＮＰＴⅡ的酶活检测对获得的转基因烟草进行了鉴定．Ｎｏｒｔｈｅｒｎ分析表明：ＣａＭＶ３５Ｓ启动子驱动下的Ｔ－ｃｙｔ基因在转基因烟草的根、茎、叶中均有ｍＲＮＡ的积累；ｒｂｃＳ启动子指导下Ｔ－ｃｙｔ基因在叶中转录较强，茎中次之，在根中几乎未表达．以根据抗原决定簇进行人工合成的复合抗原免疫家兔得到异戊烯基转移酶抗体，ＥＬＩＳＡ结果表明转基因烟草根中异戊烯基转移酶含量较高．Ｔ－ｃｙｔ基因的表达对转基因烟草的生长发育有明显影响：其叶绿素ａ、ｂ含量明显增加，叶衰老迟缓；顶端优势受到抑制，侧芽生长旺盛；与对照相比，其根系不发达．     

用地下水资料探讨渭河盆地及周边地区地震形势

本文用陕、甘、宁、青的地下水前兆观测资料，运用异常系列方法的思路，研究了四省区中强地震的中期前兆指标，并进一步探讨了渭河盆地及周边的地震形势。     

自制固相微萃取装置-气相色谱法测定空气中痕量苯

采用自制的固相微萃取(SPME)装置结合气相色谱仪(GC)对室内空气中痕量苯进行定性和定量的分析。阐述了固相微萃取法的原理和定量的机理;对影响SPME的因素(空气柱、萃取时间、解吸时间和温度)进行了讨论;对所建立的方法进行了系统评价,结果表明:建立的方法具有良好的线性关系(相关系数R=0.9998),检出限为0.01mg/L,3个含量点的相对标准偏差在允许的范围7%以内,回收率为98.18%。     

浅析职业服装的分类及设计

1 前言 职业服装是融标志性、功能性、时尚性、实用性及科学性于一体并具有行业特点和职业特征．能满足职业功能，体现团队精神和服饰文化的标志性服装。     

天津市区县间大气污染相互传输贡献研究

灰霾污染是当前天津市面临的首要大气污染问题。应用MM5/CMAQ模型,选取1月和7月作为冬、夏两季典型代表月份,采用源开关法,对天津市各区县之间的PM2.5污染相互传输贡献进行识别分析。结果表明,天津市各区县间的相互污染影响具有较显著的季节变化和区域分布特点。在冬夏两季的北辰和滨海新区,50%以上污染都来自于本区域贡献。冬季,中心城区和平、河东、河西、南开、河北约30%的污染来自于本区域贡献;夏季,中心城区来自本区域贡献大幅减少,而静海、宁河、津南及东丽等区来自本区域贡献比重较冬季有所增加。总体而言,滨海新区和北辰区主要受本地污染源影响,其他区域受外部源贡献影响较大。研究结果为筛选重点控制区域,制定城市大气污染治理控制方案提供科学依据。     

Distribution characteristics and indicator significance of Dechloranes in multi-matrices at Ny-Ålesund in the Arctic

In recent years, Dechloranes have been widely detected in the environment around the world. However, understanding and knowledge of Dechloranes in remote regions, such as the Arctic, remain lacking. Therefore, the concentrations of 5 Dechloranes in surface seawater, sediment, soil, moss, and dung collected from Ny-Ålesund in the Arctic were measured with the concentrations 93 pg/L, 342, 325, 1.4, and 258 pg/g, respectively, which were much lower than those in Asian and European regions. The mean ratios of anti-Dechlorane Plus (DP) to total DP (?_anti) in seawater, sediment, soil, moss, dung, and atmospheric samples were 0.36, 0.21, 0.18, 0.27, 0.66, and 0.43, respectively. Results suggested that the main source of DP in seawater, sediment, soil, and moss was long-range atmospheric transport. However, the ratio identified in dung was different, for which the migration behavior of the organism is probably the main source of DP.     

Distribution characteristics and indicator significance of Dechloranes in multi-matrices at Ny-Ålesund in the Arctic

In recent years, Dechloranes have beenwidely detected in the environment around the world. However, understanding and knowledge of Dechloranes in remote regions, such as the Arctic, remain lacking. Therefore, the concentrations of 5 Dechloranes in surface seawater, sediment, soil, moss, and dung collected from Ny-Ålesund in the Arctic were measured with the concentrations 93 pg/L, 342, 325, 1.4, and 258 pg/g, respectively, which were much lower than those in Asian and European regions. The mean ratios of anti-Dechlorane Plus (DP) to total DP (f_anti) in seawater, sediment, soil, moss, dung, and atmospheric samples were 0.36, 0.21, 0.18, 0.27, 0.66, and 0.43, respectively. Results suggested that the main source of DP in seawater, sediment, soil, andmosswas long-range atmospheric transport. However, the ratio identified in dung was different, for which the migration behavior of the organism is probably themain source of DP.     

与地裂缝正交马蹄形地铁隧道动力响应模拟分析

在地铁隧道—地裂缝动力相互作用模型试验中,拟采用点荷载的加载方式。为了合理制定模型试验加载方案,建立了点荷载和移动荷载2种加载方式下,与地裂缝正交的马蹄形隧道—地裂缝—地层动力相互作用的数值模型。通过对2种加载方式下的拱底土层竖向最大位移、土层最大加速度分布、土层加速度时程以及衬砌加速度时程进行对比分析,可以得出在2种加载方式下,拱底竖向最大位移、土层加速度分布、土层加速度时程以及衬砌加速度时程在分布上有相近的规律,从而说明了在模型试验中,可以用点荷载加载方式来代替移动荷载,为模型试验提供了依据。     

黄泔水发酵微生物絮凝剂B-12的研究

以黄泔水为廉价培养基,对絮凝剂产生菌B-12进行发酵培养,用单因素实验和正交设计试验,考察了培养基条件对絮凝剂产生菌絮凝效果的影响。结果表明:B-12的优化培养条件为:黄泔水体积分数为12.5%,硫酸铵为0.5g/L,温度为28℃,初始培养pH为6.5。在此条件下所产生的絮凝剂对高岭土悬液絮凝率高达81.2%。利用黄泔水生产的微生物絮凝剂B-12对化粪池废水、城市生活污水和土壤悬浊液具有良好的絮凝效果。     

湿式电除尘器伏安特性的实验研究

伏安特性是分析电除尘放电特性和运行状况的基本方法。深入研究电除尘伏安特性,有助于提高除尘效率,为微细颗粒物控制提供了理论基础。实验研究了不同电场风速、雾滴粒径和喷水量下湿式电除尘的伏安特性。结果表明:当电场风速为1.0～1.2m/s、喷嘴压力为0.3～0.4MPa、喷水流量为0.24m3/h,湿式电除尘器处于最佳工作状态,通过对该状态下的除尘效率的测定,验证了该参数组合的正确性。