2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor

Emerging evidence showed that 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) could induce expression of certain reactivation-associated genes in astrocytes, however, the consequent cellular effects and molecular mechanisms are still unclear. During the process of astrocyte reactivation, migration is a critical cellular event. In the present study, we employed wound-healing assay and Transwell® motility assay to explore the effects of TCDD on cell migration in primary cultured rat cortical astrocytes. We found that upon TCDD treatments at relative low concentrations (10^? 10 and/or 10^? 9?mol/L), the ability of primary astrocytes to migrate horizontally and vertically was promoted. In line with this cellular effect, the mRNA expression of two pro-migratory genes, including cell division cycle 42 (CDC42) and matrix metalloproteinase 2 (MMP2) was induced by TCDD treatment. Dioxin exerts its toxic effects mainly through aryl hydrocarbon receptor (AhR) pathway. So the role of AhR pathway in the pro-migratory effects of TCDD was examined using an AhR antagonist, CH223191. We found that application of CH223191 significantly reversed the pro-migratory effects of TCDD. Interestingly, the basal ability of horizontal migration as well as basal levels of CDC42 and MMP2 expression were dramatically reduced suggesting a possible physiological role of AhR in maintaining the endogenous migration ability of the primary astrocytes. These findings support the notion that dioxin promotes astrocyte reactivation at molecular and cellular levels.     

The thermal transformation mechanism of chlorinated paraffins: An experimental and density functional theory study

The increasing production and usage of chlorinated paraffins(CPs) correspondently increase the amount of CPs that experience thermal processes. Our previous study revealed that a significant amount of medium-chain chlorinated paraffins(MCCPs), short-chain chlorinated paraffins(SCCPs) as well as aromatic and chlorinated polycyclic aromatic hydrocarbons(Cl-PAHs) were formed synergistically during the thermal decomposition of CP-52(a class of CP products).However, the transformation mechanisms of CP-52 to these compounds are still not very clear.This article presents a mechanistic analysis on the decomposition of CP-52 experimentally and theoretically. It was found that CP-52 initially undergoes dehydrochlorination and carbon chain cleavage and it transformed into chlorinated and unsaturated hydrocarbons. Cyclization and aromatization were the most accessible pathways at low temperatures(200–400°C), both of which produce mostly aromatic hydrocarbons. As the temperature exceeds 400°C, the hydrocarbons could decompose into small molecules, and the subsequent radical-induced reactions become the predominant pathways, leading to the formation of Cl-PAHs. The decomposition of CP-52 was investigated by using density functional theory and calculations demonstrating the feasibility and rationality of PCB and PCN formation from chlorobenzene. The results improve the understanding of the transformation processes from CP-52 to SCCPs and Cl-PAHs as well as provide data for reducing their emissions during thermal-related processes.     

Impact of dissolved organic matter on the photolysis of the ionizable antibiotic norfloxacin

Norfloxacin (NOR), an ionizable antibiotic frequently used in the aquaculture industry, has aroused public concern due to its persistence, bacterial resistance, and environmental ubiquity. Therefore, we investigated the photolysis of different species of NOR and the impact of a ubiquitous component of natural water — dissolved organic matter (DOM), which has a special photochemical activity and normally acts as a sensitizer or inhibiter in the photolysis of diverse organics; furthermore, scavenging experiments combined with electron paramagnetic resonance (EPR) were performed to evaluate the transformation of NOR in water. The results demonstated that NOR underwent direct photolysis and self-sensitized photolysis via hydroxyl radical (·OH) and singlet oxygen (¹O₂) based on the scavenging experiments. In addition, DOM was found to influence the photolysis of different NOR species, and its impact was related to the concentration of DOM and type of NOR species. Photolysis of cationic NOR was photosensitized by DOM at low concentration, while zwitterionic and anionic NOR were photoinhibited by DOM, where quenching of UOH predominated according to EPR experiments, accompanied by possible participation of excited triplet-state NOR and ¹O₂. Photo-intermediate identification of different NOR species in solutions with/without DOM indicated that NOR underwent different photodegradation pathways including dechlorination, cleavage of the piperazine side chain and photooxidation, and DOM had little impact on the distribution but influenced the concentration evolution of photolysis intermediates. The results implied that for accurate ecological risk assessment of emerging ionizable pollutants, the impact of DOM on the environmental photochemical behavior of all dissociated species should not be ignored.     

地质勘探巷道的通风新方法探讨

探讨了巷道通风的新方法，其中包括用间断风筒的通风、半循环通风、封闭式通风、高压通风机通风等，并就通风研究的发展方向提出了几点建议     

火工品可靠性(或安全性)评估的Bayes方法

对火工品可靠性（或安全性）评估中通常采用的经典方法进行了剖析。介绍了数理统计方法学中近年来兴起的Ｂａｙｅｓ统计推断方法，并给出了此法在火工品二项分布、正态分布情况下，对可靠度评估的应用实例。     

深水淹没高喷注浆帷幕技术

介绍导孔钻进、旋喷注浆成桩、桩顶沉降回灌等三重管高压旋喷注浆技术、参数、设备、工艺及其在上海市杨树浦水厂改建工程中的成功应用。该技术对保证安全、优质、高效的施工发挥了重要作用。     

三维电极电催化氧化法处理废水的研究进展

综述了近几年国内外在三维电极电化学反应器方面的最新研究进展,分析了电源的输出形式、阴阳极板的布置方式、粒子电极的填充方式等因素对废水处理效果的影响,探讨了碳纳米材料等新材料在粒子电极载体上的应用,最后介绍了三维电极法与其他技术联用的最新研究,并指出了三维电极法存在的问题及今后的研究方向。     

城市轨道交通火灾事故风险评估及对策措施

运用故障树安全评价的方法和火灾风险评估中的定量分析方法,以人员安全为出发点,对轨道交通运营过程火灾事故风险进行评估。并对重庆市轻轨2号线临江门车站进行紧急情况下人员疏散计算,判断轻轨临江门车站发生火灾等紧急事故时,能否确保乘客的人身安全。结合轨道交通现状给出相应对策措施,降低事故发生的可能性,确保乘客生命安全和财产不受损失。     

Using game theory to describe strategy selection for environmental risk and carbon emissions reduction in the green supply chain

This paper provides an approach in the context of green supply chain management, using game theory to analyze the strategies selected by manufacturers to reduce life cycle environmental risk of materials and carbon emissions. Through the application of the ‘tolerability of risk’ concept, a basis for determining the extent of environmental risk and carbon emissions reduction has been established. Currently, scant attention is given to holistic supervision of the supply chain with respect to carbon emissions by governments, and thus the starting hypothesis here is that the default strategy that manufacturers will adopt is only to reduce carbon emissions, and thereby environmental risk, in so far as this is compatible with the aim of increasing revenue. Moreover, we further hypothesize that, once necessary governmental policy has been established in the supply chain management, the strategic choices of the manufacturers would be influenced by government penalties or incentives. A case example is provided to demonstrate the insight that indicates the application of game theory. The limitations of the game model and analysis are discussed, laying a foundation for further work.     

上覆岩层瓦斯卸压范围及流动规律的应用研究

为合理、高效地治理朱家店煤矿巷道掘进和煤层开采过程中瓦斯浓度多次超限的技术阻力,本文针对该煤矿不同煤层上覆岩层瓦斯卸压范围及流动规律进行了较为深入的研究,建立了其BP神经网络破坏高度的数学预测模型。通过合理地计算,本文确定了该矿开采煤层上覆岩层的走向及倾向卸压长度、卸压上限和卸压范围,最后提出了有效控制上覆岩层瓦斯大量流向工作面及采空区的技术策略,从而达到了遏制矿井瓦斯事故发生的目的。