Quantification of human amniotic fluid constituents by high resolution proton nuclear magnetic resonance (NMR) spectroscopy

We have investigated the ability of high-resolution proton NMR spectroscopy to provide a biochemical constituent screening of human amniotic fluid (AF). Proton NMR spectra were obtained at 300 MHz on AF from patients undergoing amniocentesis in the mid-trimester. Only AF from normal pregnancies (normal fetal karyotype, normal a-fetoprotein levels, normal birth outcome) was used in this study. The AF supernatant was lyophilized and resuspended in deuterated water containing 0.1 mm phosphate buffer and 6.02 mm disodium maleate. Identification of low molecular weight compounds was confirmed by two-dimensional NMR spectra (primarily correlated spectroscopy, or COSY) and standard addition techniques. A broad profile of compounds were ‘NMR visible’ in a single proton spectrum, including creatinine, glucose, organic acids (acetate, citrate, and lactate) and several amino acids (alanine, histidine, leucine, phenylalanine, tyrosine and valine). The proton spectrum was unaffected by prior freezing/thawing of AF samples. We were able to quantify compounds by comparison with an added concentration standard (maleate) at concentrations as low as 30 μm. Good agreement with literature values based on other analytical techniques was obtained.     

Der Einfluß von Verbraucher und Handel auf die Qualität landwirtschaftlicher Produkte und Lebensmittel

The modern consumer is very much interested in healthy food. The reason is that a special group of consumers is focusing more and more attention on health and nutrition problems. Further, there is increased emphasis on education in food science at universities as well as polytechnics. For some years worldwide dietary goals are published with the intention to reduce nutrition-based sickness. For the first time in our nutrition report 1988, clear proposals for optimal nutrition were formulated.     

Der Strahlungshaushalt der terrestrischen Planeten Venus,Erde und Mars

The radiation budget of planets substantially determines their climates. For the terrestrial planets having their own imperceptible heat source compared to the solar radiation, the radiation budget supplies energy that can be transformed and used in their atmospheres and at their surfaces. The crucial role of the radiation budget for the Earth's climate is manifested in current international programs like the World Climate and Global Change Programs. The intercomparison and analysis of the radiation budget of the terrestrial planets, due to quite different atmospheric compositions and external conditions, provide a deeper insight into the relations of the different components of the radiation budget.