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181.
Mónica Martínez-Martínez Marta Diez-Valcarce Marta Hernández David Rodríguez-Lázaro 《Food and environmental virology》2011,3(2):92-98
Synthetic multiple-target RNA and DNA oligonucleotides were constructed for use as quantification standards for nucleic acid
amplification assays for human norovirus genogroup I and II, hepatitis E virus, murine norovirus, human adenovirus, porcine
adenovirus and bovine polyomavirus. This approach overcomes the problems related to the difficulty of obtaining practical
quantities of viral RNA and DNA from these viruses. The quantification capacity of assays using the standards was excellent
in each case (R
2 > 0.998 and PCR efficiency > 0.89). The copy numbers of the standards were equivalent to the genome equivalents of representative
viruses (murine norovirus and human adenovirus), ensuring an accurate determination of virus presence. The availability of
these standards should facilitate the implementation of nucleic acid amplification-based methods for quantitative virus detection. 相似文献
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184.
Studies of the Slovakian Danube have indicated profound changes in the structures of several invertebrate assemblages in the section of the river bypassed by the Gab?íkovo hydroelectric project in 1992 and in adjacent floodplain water bodies. The present study investigates the relationship between hydrological connectivity and species diversity in the old main channel and adjacent left-bank water bodies, using resident cladocerans as indicators of the ecological integrity of the river-floodplain system. The work aimed to quantify cladoceran habitat preferences using habitat values (HV) and indication weights (IW) calculated from data accumulated during long-term monitoring of planktonic communities of the Danube floodplain (1,840.5–1,804 rkm) and supplemented with data from the literature. Although not listed as an indicator group in the European Union Water Framework Directive, cladocerans are shown to be highly apposite in the ecological assessment of river-floodplain systems. Of 71 recorded cladoceran species, 19 taxa showed a preference for eupotamal habitats, 24 species preferred eupotamal B/parapotamal waters, and 28 cladocerans were found to prefer the plesiopotamal/paleopotamal habitat type. Identified habitat types follow a gradient of hydrological connectivity with the main river channel, ranging from the eupotamal to more or less isolated floodplain water bodies. A secondary aim was to use the calculated values to assess the ecological integrity of the river-floodplain system. The key element of this procedure is the calculation of the Floodplain Index (FI) (Chovanec et al. Large Rivers, 15(1–4), 169–185 2005) from the summation of the habitat values and indication weights of all species present at the sampling sites. Calculated index values indicate the extent of disruption to lateral connectivity in the floodplain area. 相似文献
185.
Mirian R. Calderon Patricia González Marta Moglia Soledad Oliva Gonzáles Mariana Jofré 《Environmental monitoring and assessment》2014,186(7):4411-4422
Urbanization can cause significant changes in the integrity of fluvial ecosystems, which makes it necessary to assess environmental conditions of areas where population growth rates are high. A study of the environmental quality of Chorrillos River (San Luis-Argentina) and its tributaries was carried out in order to evaluate the potential effect of an urbanization gradient. Six sites were sampled along the main course and tributaries of the river. Urbanization variables were measured and included to calculate an Urbanization Index. Physical–chemical analyses were performed in water samples to evaluate water quality through the use of a simplified index of water quality (SIWQ). Plants, macroinvertebrates, and amphibians metrics were used to assess the biological state of the studied sites. The Urbanization Index varied significantly between sites and was significantly correlated to the SIWQ. However, no significant correlations were found between SIWQ and macroinvertebrates and amphibians variables. Water quality of Chorrillos River and its tributaries is good, but it is affected by anthropic influences as reflected by the declining of SIWQ values. Although biological sampling constitutes an important tool in the assessment of water quality of rivers, in this report biological results were not conclusive. 相似文献
186.
Anicuta Stoica-Guzun Luiza Jecu Amalia Gheorghe Iuliana Raut Marta Stroescu Marius Ghiurea Mihai Danila Iuliana Jipa Victor Fruth 《Journal of Polymers and the Environment》2011,19(1):69-79
The ability of fungal strains to attack a composite material obtained from poly(vinyl alcohol) (PVA) and bacterial cellulose
(BC) is investigated. The fungal strain tested was Aspergillus niger. This fungal strain was able to change not only the polymer surface from smoother to rougher, but also to disrupt the polymer.
The degradation results were confirmed by visual observations, scanning electron microscopy (SEM) analyses, X-ray diffraction
analyses and FTIR spectra of the film samples. SEM micrographs confirmed the growth of fungi on the composite film surface.
The degree of microbial degradation depends on culture medium and on composition of polymeric materials, especially on PVA
content. The biodegradation process is accelerated by the presence of glucose in the culture medium as an easily available
carbon source. 相似文献
187.
Fitzgerald EF Shrestha S Palmer PM Wilson LR Belanger EE Gomez MI Cayo MR Hwang SA 《Chemosphere》2011,85(2):225-231
A study was conducted to evaluate the association between PCBs in residential indoor air and in the serum of older, long time residents of three upper Hudson River communities. Samples of indoor air and of serum were collected from 170 persons 55 to 74 years of age, and analyzed for PCBs using glass capillary gas chromatography. After adjusting for age, BMI, cigarette smoking, and Hudson River fish consumption with multiple linear regression analysis, the results indicated statistically significant associations between concentrations in indoor air and serum for PCB-28, a lightly chlorinated congener common in air that accumulates in serum, and PCB-105. Duration of exposure was an important factor, since among persons who had lived in their home for 39 years or more, 11 of the 12 most commonly detected congeners were significantly correlated, as was their sum (∑ PCB). Significant associations between indoor air and serum PCB concentrations also were more likely when collected in cooler months and if the two samples were collected within 20 d of each other. The study is among the first to indicate that PCB concentrations characteristic of residential indoor air are associated with a detectable increase in body burden. 相似文献
188.
Marta Doval Miñarro Enrique González Ferradás Jonathan Barberá Rico Fernando Díaz Alonso Francisco J. Marzal Martínez Cristina Romero Trigueros 《Environmental science and pollution research international》2011,18(3):436-445
Introduction
The change in light intensity that takes place when an ambient air sample is drawn into the detection chamber of a chemiluminescence monitor generates changes in the concentrations of several species, such as NO2, NO and O3. Although this phenomenon has been known for several decades, there is still no commonly accepted approach on when or how to correct for it in NO2 and O3 readings. 相似文献189.
Since 2002, the chemical/petrochemical industrial zone of Tarragona County (Catalonia, Spain) is being annually monitored. As part of the environmental surveillance program, in this study the levels of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), polycyclic aromatic hydrocarbons (PAHs), and metals (As, Cd, Cr, Hg, Mn, Pb and V) were determined in soil and vegetation samples collected in 4 areas of Tarragona County (chemical, petrochemical, urban/residential, and unpolluted). Moreover, the airborne concentrations of the same micropollutants were determined in each area. In soil samples, significant higher levels of PCNs and higher concentrations of PCDD/Fs and PAHs were found in the urban zone. PCDD/F levels in vegetation samples significantly decreased from 2002. The concentrations of Cr in soil samples, as well as V levels in vegetation samples collected in the vicinity of an oil refinery were significantly higher than those found in the unpolluted zones. A significant and progressive increase in V concentrations was also noted. The current results clearly indicate that the petrochemical industry is still being an important focus of inorganic pollution for the surrounding environment. In air, the higher amount of the 7 carcinogenic PAHs suggests a relatively greater impact on the petrochemical and urban areas. The temporal trend of the global pollution was also studied an Integral Risk Index was applied. 相似文献
190.
Palma Orlović-Leko Marta Plavšić Elvira Bura-Nakić Zlatica Kozarac Božena Ćosović 《Atmospheric environment (Oxford, England : 1994)》2009,43(4):805-811
Dissolved organic carbon (DOC), surface active substances (SAS) and copper complexing capacity (CuCC) were studied in bulk precipitations collected periodically from 2003 to 2007 in the continental city of Croatia (Zagreb: n = 27) and in the city at the Adriatic coast (?ibenik: n = 38). DOC concentrations (Zagreb: 0.67–4.03 mgC/L with average concentration of 1.93 ± 0.76 mgC/L; ?ibenik: 0.44–4.13 mgC/L with average concentration of 1.83 ± 0.83 mgC/L) are similar to those measured in other samples of continental rainwater in the northern hemisphere. The concentrations of SAS in samples from Zagreb ranged from 0.055 to 0.45 eq. Triton-X-100 mg/L with average concentration of (0.14 ± 0.06) eq. Triton-X-100 mg/L. SAS fractions were of a similar range in ?ibenik (0.02–0.60 eq. Triton-X-100 mg/L) with an average concentration of 0.11 ± 0.06 eq. Triton-X-100 mg/L. However, the lowest values of SAS (between 0.02 and 0.04 eq. Triton-X-100 mg/L) were observed only in ?ibenik (27%). We have estimated that the higher pH values were responsible for lower surface activity of organic matter in bulk samples from ?ibenik. DOC may form complexes that control the transport and solubility of heavy metals in natural water. CuCC measured in ?ibenik in the range 0.066–1.4 μM Cu2+ was in general higher, compared to the one in Zagreb (0.010–0.586 μM Cu2+) which is the result of biogenically driven organic contribution to the precipitation, especially in the warmer period of the year. 相似文献