Temperature and salinity: two climate change stressors affecting early development of the New Zealand sea urchin Evechinus chloroticus

Temperature and salinity are important environmental factors affecting the normal functioning of marine animals, particularly animals such as sea urchins living in shallow waters and tide pools. Here, we evaluated the effect of different combinations of temperature and salinity on early embryos of the endemic New Zealand sea urchin Evechinus chloroticus. Animals were collected at Matheson’s Bay (36º18′17′′S; 174º47′51′′E) in north-eastern New Zealand in February 2013. Embryos were exposed to five salinities (29, 31, 34, 35 and 37 ppt) and two temperatures (18 and 21 °C) during the first 24 h of development. Low salinity (29 ppt) affected all parameters (fertilization, development rate, gastrulation and normal development), with ca. 50 % of embryos surviving at 29 ppt, whereas seawater temperature only affected development rate and gastrulation. An increase in temperature from 18 to 21 °C minimized the negative effect of low salinity (≤31 ppt) on development rate and gastrulation of E. chloroticus. Overall, the results of this study suggest that early embryos of E. chloroticus have developmental plasticity to withstand reductions in salinity up to 29 ppt; however, it is still unknown whether the surviving embryos will be able to complete larval development at low salinities, particularly whether the embryos and larvae are carried into extreme environments such as estuaries where salinity is even lower. Multistressor studies are very important for climate change research as multiple environmental factors will act together in the wild, having major consequences for development and recruitment of marine invertebrates.     

Who counts in evaluating the effects of air pollution policies on households? Non-market valuation in the presence of dependencies

Individuals who are likely to realize the largest benefits from improvements in air quality often depend on other members of their households to make time or monetary contributions to their care. The presence of these dependency relationships among household members poses challenges for benefit estimation since it is unlikely that the conditions necessary for recovering the underlying individual preferences from household choices are satisfied in this setting. We propose a conceptual framework that highlights the role of these dependencies in the choice models used to estimate the willingness to pay for environmental quality improvements. We design a complementary stated preference survey that describes hypothetical dependency relationships for household members of different ages to test the implications of our conceptual model. Respondents' stated choices take into account the proposed care-giving responsibilities for young children and teenagers but not for older adults.     

Developing safer passengers through a school-based injury prevention program

Motor vehicle crashes are a leading cause of death among young people. Fourteen percent of adolescents aged 13–14 report passenger-related injuries within three months. Intervention programs typically focus on young drivers and overlook passengers as potential protective influences. Graduated Driver Licensing restricts passenger numbers, and this study focuses on a complementary school-based intervention to increase passengers’ personal- and peer-protective behavior. The aim of this research was to assess the impact of the curriculum-based injury prevention program, Skills for Preventing Injury in Youth (SPIY), on passenger-related risk-taking and injuries, and intentions to intervene in friends’ risky road behavior. SPIY was implemented in Grade 8 Health classes and evaluated using survey and focus group data from 843 students across 10 Australian secondary schools. Intervention students reported less passenger-related risk-taking six months following the program. Their intention to protect friends from underage driving also increased. The results of this study show that a comprehensive, school-based program targeting individual and social changes can increase adolescent passenger safety.     

Physiological responses of horseshoe crab (<Emphasis Type="Italic">Limulus polyphemus</Emphasis>) embryos to osmotic stress and a possible role for stress proteins (HSPs)

We tested the effects of osmotic stress on survival, developmental rate, and level of HSPs on American horseshoe crab (Limulus polyphemus) embryos. Animals were maintained in the laboratory at an ambient salinity of 20 ppt and then exposed to 4-h osmotic shocks at salinities of 10, 30, 40, 50, and 60 ppt, with a control group at 20 ppt. Horseshoe crab embryos had 100% developmental success (defined as individuals reaching the first instar or trilobite larval stage) at all salinities. However, osmotic stresses, especially hyperosmotic conditions, slowed the rate of development. Embryos subjected to osmotic stress showed higher levels of HSP70 and HSP90 than control animals kept at a salinity of 20 ppt. HSPs are of value to horseshoe crab embryos in surviving the fluctuating salinities that are typical of estuarine beach habitats.     

Evaluation of alternative approaches for screening contaminated sediments and soils for polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans

Traditional high resolution mass spectrometry (HRMS) analysis for polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) can be time consuming and expensive. Consequently, alternative methods are of great interest to regulatory agencies and others characterizing contaminated sites. One factor that hinders acceptance of alternative methods is a lack of performance information that assesses the alternative method's impacts on analytical results. The U.S. Environmental Protection Agency's Superfund Innovative Technology Evaluation Monitoring and Measurement Technologies Program (EPA SITE MMT) encourages the development and implementation of innovative and alternative monitoring methods by providing performance information on site characterization technologies. This paper presents a comparison of the results obtained from laboratory-based alternative approaches for screening sediment and soil samples for dioxin toxicity equivalents (TEQ(D/F)) to results obtained using traditional HRMS. The laboratory-based approaches included modifying the traditional HRMS analysis to make it more cost-effective (alternate 1613B), analyzing extracts that had been prepared for HRMS using low resolution mass spectrometry, and determining total organic carbon (TOC) content as an indicator of PCDD/F content. These comparisons demonstrated that TEQ(D/F) values generated using toxicity equivalency factors proposed by the World Health Organization in 1998 applied to alternate 1613B and LRMS analyses have a strong linear correlation to the TEQ(D/F) values derived in the same fashion from traditional HRMS analysis. These results would have placed >90% of the samples within the same concentration intervals using ranges of <0.05, 0.05-0.50, 0.50-5, and >5ng TEQ/g. Natural log transformed data for TOC had significantly weaker correlation to TEQ(D/F), indicating that TOC is not a reliable indicator of TEQ(D/F) concentrations.     

Analyses of Urban Drainage Network Structure and its Impact on Hydrologic Response1

Meierdiercks, Katherine L., James A. Smith, Mary Lynn Baeck, and Andrew J. Miller, 2010. Analyses of Urban Drainage Network Structure and Its Impact on Hydrologic Response. Journal of the American Water Resources Association (JAWRA) 1-12. DOI: 10.1111/j.1752-1688.2010.00465.x Abstract: Urban flood studies have linked the severity of flooding to the percent imperviousness or land use classifications of a watershed, but relatively little attention has been given to the impact of urban drainage networks on hydrologic response. The drainage network, which can include storm pipes, surface channels, street gutters, and stormwater management ponds, is examined in the Dead Run watershed (14.3 km²). Comprehensive digital representations of the urban drainage network in Dead Run were developed and provide a key observational resource for analyses of urban drainage networks and their impact on hydrologic response. Analyses in this study focus on three headwater subbasins with drainage areas ranging from 1.3 to 1.9 km² and that exhibit striking contrasts in their patterns and history of development. It is shown that the drainage networks of the three subbasins, like natural river networks, exhibit characteristic structures and that these features play critical roles in determining urban hydrologic response. Hydrologic modeling analyses utilize the Environmental Protection Agency’s Stormwater Management Model (SWMM), which provides a flexible platform for examining the impacts of drainage network structure on hydrologic response. Results of SWMM modeling analyses suggest that drainage density and presence of stormwater ponds impact peak discharge more significantly in the Dead Run subbasins than the percent impervious or land use type of the subbasins.     

Urban–rural differences in atmospheric mercury speciation

Measurements of gaseous elemental mercury (GEM), particulate mercury (Hg_p), and reactive gaseous mercury (RGM) were concurrently recorded at an urban site in Detroit and a rural site in Dexter, both in Michigan for the calendar year 2004. Their average concentrations (±standard deviation) for the urban area were 2.5 ± 1.4 ng m^?3, 18.1 ± 61.0 pg m^?3, and 15.5 ± 54.9 pg m^?3, respectively, while their rural counterparts were 1.6 ± 0.6 ng m^?3, 6.1 ± 5.5 pg m^?3, and 3.8 ± 6.6 pg m^?3, respectively. The medians of urban-to-rural ratios of Hg concentrations indicate approximately 1-fold, 2-fold, and 3-fold gradients between Detroit and Dexter for GEM, Hg_p, and RGM, respectively. The urban–rural differences in Hg also varied considerably on different temporal scales and with wind flow patterns, which was most evident in RGM. Our results show that while Hg at both sites was impacted by regional sources, meteorological conditions, and photochemical transformations, the extent of variations in the observed urban-to-rural gradients, particularly in RGM, cannot be fully accounted for by these processes. Both analyses of the annual data and case studies indicate that the more variable and episodic nature of Hg, particularly RGM, seen in Detroit compared with Dexter, was the result of direct impact from local anthropogenic sources.