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581.
We tested the effects of osmotic stress on survival, developmental rate, and level of HSPs on American horseshoe crab (Limulus polyphemus) embryos. Animals were maintained in the laboratory at an ambient salinity of 20 ppt and then exposed to 4-h osmotic shocks
at salinities of 10, 30, 40, 50, and 60 ppt, with a control group at 20 ppt. Horseshoe crab embryos had 100% developmental
success (defined as individuals reaching the first instar or trilobite larval stage) at all salinities. However, osmotic stresses,
especially hyperosmotic conditions, slowed the rate of development. Embryos subjected to osmotic stress showed higher levels
of HSP70 and HSP90 than control animals kept at a salinity of 20 ppt. HSPs are of value to horseshoe crab embryos in surviving
the fluctuating salinities that are typical of estuarine beach habitats. 相似文献
582.
Jodi D. Hoffman Diana W. Bianchi Lisa M. Sullivan Brenda L. Mackinnon Jamie Collins Fergal D. Malone T. Flint Porter David A. Nyberg Christine H. Comstock Radek Bukowski Richard L. Berkowitz Susan J. Gross Lorraine Dugoff Sabrina D. Craigo Ilan E. Timor-Tritsch Stephen R. Carr Honor M. Wolfe Mary E. D'Alton 《黑龙江环境通报》2008,28(13):1204-1208
583.
584.
Andressa Gonsioroski Mary Laws Vasiliki E. Mourikes Alison Neff Jenny Drnevich Michael J. Plew Jodi A. Flaws 《环境科学学报(英文版)》2022,34(7):46-57
Iodoacetic acid (IAA) is an unregulated water disinfection byproduct that is an ovarian toxicant. However, the mechanisms of action underlying IAA toxicity in ovarian follicles remain unclear. Thus, we determined whether IAA alters gene expression in ovarian follicles in mice. Adult female mice were dosed with water or IAA (10 or 500 mg/L) in the water for 35-40 days. Antral follicles were collected for RNA-sequencing analysis and sera were collected to measure estradiol. RNA-sequencing analysis identified 1063 differentially expressed genes (DEGs) in the 10 and 500 mg/L IAA groups (false discovery rate FDR < 0.1), respectively, compared to controls. Gene Ontology Enrichment analysis showed that DEGs were involved with RNA processing and regulation of angiogenesis (10 mg/L) and the cell cycle and cell division (500 mg/L). Pathway Enrichment analysis showed that DEGs were involved in the phosphatidylinositol 3-kinase and protein kinase B (PI3K-Akt), gonadotropin-releasing hormone (GnRH), estrogen, and insulin signaling pathways (10 mg/L). Pathway Enrichment analysis showed that DEGs were involved in the oocyte meiosis, GnRH, and oxytocin signaling pathways (500 mg/L). RNA-sequencing analysis identified 809 DEGs when comparing the 500 and 10 mg/L IAA groups (FDR < 0.1). DEGs were related to ribosome, translation, mRNA processing, oxidative phosphorylation, chromosome, cell cycle, cell division, protein folding, and the oxytocin signaling pathway. Moreover, IAA exposure significantly decreased estradiol levels (500 mg/L) compared to control. This study identified key candidate genes and pathways involved in IAA toxicity and can help to further understand the molecular mechanisms of IAA toxicity in ovarian follicles. 相似文献
585.
Rachowicz LJ Knapp RA Morgan JA Stice MJ Vredenburg VT Parker JM Briggs CJ 《Ecology》2006,87(7):1671-1683
A newly discovered infectious disease of amphibians, chytridiomycosis, caused by the fungal pathogen Batrachochytrium dendrobatidis, is implicated in population declines and possible extinctions throughout the world. The purpose of our study was to examine the effects of B. dendrobatidis on the mountain yellow-legged frog (Rana muscosa) in the Sierra Nevada of California (USA). We (1) quantified the prevalence and incidence of B. dendrobatidis through repeat surveys of several hundred R. muscosa populations in the southern Sierra Nevada; (2) described the population-level effects of B. dendrobatidis on R. muscosa population abundance; and (3) compared the mortality rates of infected and uninfected R. muscosa individuals from pre- through post-metamorphosis using both laboratory and field experiments. Mouthpart inspections conducted in 144 and 132 R. muscosa populations in 2003 and 2004, respectively, indicated that 19% of R. muscosa populations in both years showed indications of chytridiomycosis. Sixteen percent of populations that were uninfected in 2003 became infected by 2004. Rana muscosa population sizes were reduced by an average of 88% following B. dendrobatidis outbreaks at six sites, but at seven B. dendrobatidis-negative sites, R. muscosa population sizes increased by an average of 45% over the same time period. In the laboratory, all infected R. muscosa developed fatal chytridiomycosis after metamorphosis, while all uninfected individuals remained healthy. In the field experiment in which R. muscosa tadpoles were caged at infected and uninfected sites, 96% of the individuals that metamorphosed at infected sites died vs. 5% at the uninfected sites. These studies indicate that chytridiomycosis causes high mortality in post-metamorphic R. muscosa, that this emerging disease is the proximate cause of numerous observed R. muscosa population declines, and that the disease threatens this species with extirpation at numerous sites in California's Sierra Nevada. 相似文献
586.
587.
Summary. Tracer feeding studies with radioactively labeled pyrrolizidine alkaloids (PAs) were performed to attain experimental information
about the specificity and mechanisms of uptake, metabolism and storage of PAs in the alkaloid sequestering ithomiine butterfly
Mechanitis polymnia. Adult butterflies easily ingest the tracers offered dissolved in a saturated sugar solution. Feeding of [14C]rinderine (free base) confirmed that M. polymnia is well adapted to sequester and maintain PAs of the lycopsamine type. Approximately 80% of the ingested radioactivity can
be recovered in methanol extracts of the butterflies over a period of at least 6 hours. Labeled rinderine is efficiently N-oxidized and transformed into a metabolite of still unknown structure. These two metabolites are formed in almost equal amounts
and account for more than 90% of total radioactivity. After four hours the toxic free base is only detectable in traces. Radioactively
labeled senecioylretronecine (free base), a PA that often accompanies PAs of the lycopsamine type in plants, is metabolized
in a different manner. The toxic free base disappeared as fast as the tertiary rinderine, but the final products which accumulated
in a stable ratio after 12 hours were mainly two polar metabolites of unknown structure; senecioylretronecine N-oxide accounts for less than 10% of total PAs. Labeled senecionine a macrocyclic PA, which never has been found in wild caught
M. polymnia is only slowly N-oxidized. In females ca 50% of the ingested senecionine is still present as free base after 24 hours, whereas under the same
conditions in males this percentage is only ca 20%. This difference in N-oxidation was the only significant sex-specific difference observed in various experiments. Larvae of M. polymnia which feed on Solanum tabacifolium, a plant that does not contain PAs, are able to sequester and partly N-oxidize labeled senecioylretronecine and senecionine. However, the storage is not very efficient; with the two tracers less
than 5% of radioactivity remained in the bodies after 24 hours.
Received 19 October 1999; accepted 24 November 1999 相似文献
588.
Eric F. Lambin Pamela Cashman Aaron Moody Bruce H. Parkhurst Mary H. Pax Crystal Barker Schaaf 《Journal of environmental management》1993,38(4)
The main objective is to review the current status of research related to the monitoring of agricultural production in the Sahel (west Africa). The Sahel suffers from frequent shortages of food. It is therefore important to have a tool to monitor environmental variables, and thus crop production, during the agricultural season. Satellite remote sensing can contribute significantly to such a system by collecting information on crops and on environmental variables at a sub-continental geographical scale and with a high temporal frequency. One part of the problem is to estimate crop acreage. The technique of area-sampling frame has to adapted to the Sahelian landscape, which is dominated by traditional farming systems. The second part is to estimate crop yields. Three main approaches exist: statistical, semi-deterministic or deterministic. The use of vegetation indices is discussed as well as techniques to derive biophysical variables from remotely-sensed data. Finally, the integration of these remote-sensing techniques with crop-growth models is discussed and some research needs are identified. It is argued that the quantitative assessment of agricultural production in the Sahel should be based on the integration of remotely-sensed data with semi-deterministic agrometeorological models. This approach will allow a regionalization of the production estimates. 相似文献
589.
590.
This paper identifies three models of person-situation interaction in an effort to conceptually clarify and integrate previous research. Statistical criteria for each model are then developed and tested within one organizational setting. Results indicate partial support for each of the three interaction models, and suggest various improvements for person-situation interaction research. 相似文献