Evaluation of soil-washing process for “unwashable” clays and silts from the palmerton zinc site

The Palmerton Zinc Site is a Superfund site in Palmerton, Pennsylvania. Its former mineral processing operations have contaminated nearby wells and soils with zinc and cadmium. Preliminary analysis of soil and dust sampling conducted in May 1991 has revealed that the dust poses a potential threat to human health. Based on the results of a previous study, which showed a high percentage of silt and clay in soils from Palmerton residential properties, it was concluded that soil washing is not likely to be a viable method to treat the soil and dust contamination in Palmerton. However, since the completion of this study, a soil-washing process for “unwashable” clays and silts has been developed. A residential soil sample from Palmerton, which had low concentrations of arsenic, cadmium, and lead, and a somewhat high concentration of zinc, was washed in a bench-scale version of this process. The results showed that the new soil-washing process for “unwashable” clays and silts may be a viable method to treat the soil and dust contamination in Palmerton, depending on the soil quality criteria concentrations selected for site cleanup.     

Genetic evidence for multiple parentage in eastern kingbirds (Tyrannus tyrannus)

Summary Electrophoretic analysis was performed on 28 families of eastern kingbirds (Tyrannus tyrannus) from northern Michigan to estimate the occurrence of multiple parentage. Out of 19 families used in the final analysis, at least one putative parent was excluded in 9 families, or 18 out of 60 offspring (30% of offspring). Distribution of exclusion types conforms most closely to a model of quasi-parasitism, rather than extra-pair fertilizations, with secondary females laying their eggs in the primary female's nest, but random brood parasitism cannot be ruled out as an additional or alternative source of stray genes. Based on the model of random parasitism, an estimated 39% of all offspring in this population may be unrelated to one or both of the putative parents, or 53% based on a model of quasi-parasitism. Heretofore, eastern kingbirds have been considered to be exclusively monogamous; no behavioral evidence for alternative reproductive strategies has ever been reported for this species.     

Genetic Diversity in a Threatened Wetland Species, Helonias bullata (Liliaceae)

Genetic variation was examined in Helonias bullata , a threatened perennial plant species that occurs in isolated wetland habitats. Fifteen populations representing the species' geographic range were sampled. Genetic diversity was low for the species ( H _es = 0.053) as well as within populations ( H _ep = 0.029). Of the 33 allozyme loci examined, 11 (33%) were polymorphic, while on average only 12.8% (4) of the loci were polymorphic within populations. The number of alleles per polymorphic locus was 2.36 for the species and averaged 2.09 across populations. For every genetic parameter calculated, variation in H. bullata was lower than that typically found for narrowly distributed plant species. The lowest levels of genetic diversity were found in northern areas that were colonized following the last glacial epoch. The number of genotypes detected per population ranged from three to 21, with a mean of 13 for this clonally reproducing species. We found a relatively high proportion of total genetic diversity (30.6%) among populations and a significant correlation (p < 0.002) between genetic distance and geographic distance. Genetic drift phenomena appear to play a major role in the population genetics of this species. Anomalously, several populations that appeared most limited in size and vigor were genetically most variable, perhaps because they represent older, relictual populations. Life-history characteristics of H. bullata coupled with low levels of genetic diversity and the degradation and disappearance of wetlands threaten the existence of this species.     

CORRELATION OF ANNUAL PEAK FLOWS FOR PENNSYLVANIA STREAMS1

A common assumption in flood frequency analysis is that annual peak flows are independent events. This study was undertaken to investigate the validity of this assumption with regard to Pennsylvania streams by statistically analyzing the dependence between annual peak flows and to determine if basin carryover effects relate to the degree of dependence. Five tests of dependence, the autocorrelation test, the median crossing test, the turning points test, the rank difference test, and the Spearman rank order serial correlation coefficient test were applied to the series of annual peak flows for 57 streams. Of the 57 streams analyzed, only two exhibited signs of dependence by at least two of the tests performed, and the baseflow component of annual peak flows was found to be unrelated to the degree of dependence exhibited between annual peak flows. It was concluded that the assumption of independence of annual peak flows is valid in flood frequency analysis for Pennsylvania streams.     

Coyotes,deer, and wildflowers: diverse evidence points to a trophic cascade

Spatial gradients in human activity, coyote activity, deer activity, and deer herbivory provide an unusual type of evidence for a trophic cascade. Activity of coyotes, which eat young mule deer (fawns), decreased with proximity to a remote biological field station, indicating that these predators avoided an area of high human activity. In contrast, activity of adult female deer (does) and intensity of herbivory on palatable plant species both increased with proximity to the station and were positively correlated with each other. The gradient in deer activity was not explained by availabilities of preferred habitats or plant species because these did not vary with distance from the station. Does spent less time feeding when they encountered coyote urine next to a feed block, indicating that increased vigilance may contribute, along with avoidance of areas with coyotes, to lower herbivory away from the station. Judging from two palatable wildflower species whose seed crop and seedling recruitment were greatly reduced near the field station, the coyote–deer–wildflower trophic cascade has the potential to influence plant community composition. Our study illustrates the value of a case-history approach, in which different forms of ecological data about a single system are used to develop conceptual models of complex ecological phenomena. Such an iterative model-building process is a common, but underappreciated, way of understanding how ecological systems work.     

Detrimental effects of carotenoid pigments: the dark side of bright coloration

Carotenoid pigments produce yellow, orange, and red integumentary color displays that can serve as reliable signals of health and condition. In many birds and fish, individuals gain competitive or mating advantages by ingesting and utilizing large quantities of carotenoid pigments. Carotenoid pigments serve as antioxidants, performing important functions as free-radical scavengers. The beneficial effects of carotenoid pigments are well documented, but rarely have researchers considered potential detrimental effects of high-level accumulation of carotenoids. We maintained American goldfinches (Carduelis tristis) on high- or low-carotenoid diets through molt and tested for damage to the liver and skeletal muscle. High intake of carotenoids had no measurable effect on liver enzymes but caused an increase in creatine kinase, an indicator of skeletal muscle breakdown, and a reduction in vertical flight performance, a measure of skeletal muscle integrity. The detrimental effects of high-level carotenoid accumulation were approximately equivalent to the negative effects of removing carotenoids from the diet. The adverse effects observed in this study have important implications for theories of the function and evolution of colorful plumage.