Non-specific association between filamentous bacteria and fungus-growing ants

Fungus-growing ants and their fungal cultivar form a highly evolved mutualism that is negatively affected by the specialized parasitic fungus Escovopsis. Filamentous Pseudonocardia bacteria occurring on the cuticle of attine ants have been proposed to form a mutualistic interaction with these ants in which they are vertically transmitted (i.e. from parent to offspring colonies). Given a strictly vertical transmission of Pseudonocardia, the evolutionary theory predicts a reduced genetic variability of symbionts among ant lineages. The aim of this study was to verify whether actinomycetes, which occur on Acromyrmex octospinosus leaf-cutting ants, meet this expectation by comparing their genotypic variability with restriction fragment length polymorphisms. Multiple actinomycete strains could be isolated from both individual ant workers and colonies (one to seven strains per colony). The colony specificity of actinomycete communities was high: Only 15% of all strains were isolated from more than one colony, and just 5% were present in both populations investigated. Partial sequencing of 16S ribosomal deoxyribonucleic acid of two of the isolated strains assigned both of them to the genus Streptomyces. Actinomycetes could also be isolated from workers of the two non-attine ant species Myrmica rugulosa and Lasius flavus. Sixty-two percent of the strains derived from attine ants and 80% of the strains isolated from non-attine ants inhibited the growth of Escovopsis. Our data suggest that the association between attine ants and their actinomycete symbionts is less specific then previously thought. Soil-dwelling actinomycetes may have been dynamically recruited from the environment (horizontal transmission), probably reflecting an adaptation to a diverse community of microbial pathogens.     

Project house water: a novel interdisciplinary framework to assess the environmental and socioeconomic consequences of flood-related impacts

Protecting our water resources in terms of quality and quantity is considered one of the big challenges of the twenty-first century, which requires global and multidisciplinary solutions. A specific threat to water resources, in particular, is the increased occurrence and frequency of flood events due to climate change which has significant environmental and socioeconomic impacts. In addition to climate change, flooding (or subsequent erosion and run-off) may be exacerbated by, or result from, land use activities, obstruction of waterways, or urbanization of floodplains, as well as mining and other anthropogenic activities that alter natural flow regimes. Climate change and other anthropogenic induced flood events threaten the quantity of water as well as the quality of ecosystems and associated aquatic life. The quality of water can be significantly reduced through the unintentional distribution of pollutants, damage of infrastructure, and distribution of sediments and suspended materials during flood events. To understand and predict how flood events and associated distribution of pollutants may impact ecosystem and human health, as well as infrastructure, large-scale interdisciplinary collaborative efforts are required, which involve ecotoxicologists, hydrologists, chemists, geoscientists, water engineers, and socioeconomists. The research network “project house water” consists of a number of experts from a wide range of disciplines and was established to improve our current understanding of flood events and associated societal and environmental impacts. The concept of project house and similar seed fund and boost fund projects was established by the RWTH Aachen University within the framework of the German excellence initiative with support of the German research foundation (DFG) to promote and fund interdisciplinary research projects and provide a platform for scientists to collaborate on innovative, challenging research. Project house water consists of six proof-of-concept studies in very diverse and interdisciplinary areas of research (ecotoxicology, water, and chemical process engineering, geography, sociology, economy). The goal is to promote and foster high-quality research in the areas of water research and flood-risk assessments that combine and build off-laboratory experiments with modeling, monitoring, and surveys, as well as the use of applied methods and techniques across a variety of disciplines.     

Restoration planning to guide Aichi targets in a megadiverse country

Ecological restoration has become an important strategy to conserve biodiversity and ecosystems services. To restore 15% of degraded ecosystems as stipulated by the Convention on Biological Diversity Aichi target 15, we developed a prioritization framework to identify potential priority sites for restoration in Mexico, a megadiverse country. We used the most current biological and environmental data on Mexico to assess areas of biological importance and restoration feasibility at national scale and engaged stakeholders and experts throughout the process. We integrated 8 criteria into 2 components (i.e., biological importance and restoration feasibility) in a spatial multicriteria analysis and generated 11 scenarios to test the effect of assigning different component weights. The priority restoration sites were distributed across all terrestrial ecosystems of Mexico; 64.1% were in degraded natural vegetation and 6% were in protected areas. Our results provide a spatial guide to where restoration could enhance the persistence of species of conservation concern and vulnerable ecosystems while maximizing the likelihood of restoration success. Such spatial prioritization is a first step in informing policy makers and restoration planners where to focus local and large‐scale restoration efforts, which should additionally incorporate social and monetary cost–benefit considerations.     

Assessment of in situ biodegradation of monochlorobenzene in contaminated groundwater treated in a constructed wetland

The degradation of monochlorobenzene (MCB) was assessed in a constructed wetland treating MCB contaminated groundwater using a detailed geochemical characterisation, stable isotope composition analysis and in situ microcosm experiments. A correlation between ferrous iron mobilisation, decreasing MCB concentration and enrichment in carbon isotope composition was visible at increasing distance from the inflow point, indicating biodegradation of MCB in the wetland. Additionally, in situ microcosm systems loaded with 13C-labelled MCB were deployed for the first time in sediments to investigate the biotransformation of MCB. Incorporation of 13C-labelled carbon derived from the MCB into bacterial fatty acids substantiated in situ degradation of MCB. The detection of 13C-labelled benzene indicated reductive dehalogenation of MCB. This integrated approach indicated the natural attenuation of the MCB in a wetland system. Further investigations are required to document and optimise the in situ biodegradation of MCB in constructed and natural wetland systems treating contaminated groundwater.     

Irrigation of treated wastewater in Braunschweig, Germany: an option to remove pharmaceuticals and musk fragrances

In this study the fate of pharmaceuticals and personal care products which are irrigated on arable land with treated municipal wastewater was investigated. In Braunschweig, Germany, wastewater has been irrigated continuously for more than 45 years. In the winter time only the effluent of the sewage treatment plant (STP) of Braunschweig is used for irrigation, while during summer digested sludge is mixed with the effluent. In the present case study six wells and four lysimeters located in one of the irrigated agricultural fields were monitored with regard to the occurrence of 52 pharmaceuticals and two personal care products (PPCPs; e.g. betablockers, antibiotics, antiphlogistics, carbamazepine, musk fragrances, iodinated contrast media (ICM) and estrogens). No differences in PPCP pollution of the groundwater were found due to irrigation of STP effluents with and without addition of digested sludge, because many polar compounds do not sorb to sludge and lipophilic compounds are not mobile in the soil-aquifer. Most of the selected PPCPs were never detected in any of the lysimeter or groundwater samples, although they were present in the treated wastewater irrigated onto the fields. In the groundwater and lysimeter samples primarily the ICM diatrizoate and iopamidol, the antiepileptic carbamazepine and the antibiotic sulfamethoxazole were detected up to several mugl(-1), while the acidic pharmaceuticals, musk fragrances, estrogens and betablockers were likely sorbed or transformed while passing the top soil layer. Potential estrogenic effects are likely to disappear after irrigation, since the most potent steroid estrogens were not measurable.     

Multi-generation studies with Chironomus riparius--effects of low tributyltin concentrations on life history parameters and genetic diversity

While toxicological data are available for numerous chemicals from standard tests, little is known on effects of pollutants over several generations or regarding chronic effects of chemicals on genetic diversity. Within the experiments, effects of the model pollutant tributyltin (TBT) were investigated over eleven generations at a sublethal TBT concentration of 4.46 μg as Sn kg⁻¹ sediment dw on life-cycle parameters and genetic variability of Chironomus riparius. Moreover, the adaptation potential towards TBT was determined. This experimental design enables the identification of TBT effects on life-cycle parameters and the determination of a potential extinction risk caused by chronic exposure. Furthermore, effects on the genetic structure can be determined, which are not predictable based solely on knowledge of the toxic mode of action of the chemical.

Genetic variety was determined via microsatellite analysis, measuring individual length differences of highly variable satellite DNA fragments. For the identification of changes in tolerances towards the stressor, acute and chronic toxicity experiments were conducted.

During the multi-generation study, significant effects on development and reproduction were determined. For some generations, the emergence was significantly (p < 0.05) delayed under TBT exposure. Reproduction seems to be a sensitive parameter as well, whereby females laid significantly larger egg masses (p < 0.05) in the latter generations. TBT did not affect the population growth rate nor the genetic variability, while clear deviations from the Hardy–Weinberg equilibrium appeared. The study also provides strong evidence for the acquirement of a higher tolerance towards the stressor in the TBT-exposed group.     

Using slides to test for changes in crown defoliation assessment methods part II: Application of the image analysis system CROCO

We tested whether the semi-automatic program CROCO can replace visual assessments of slides to detect changes in defoliation assessment methods. We randomly selected a series of slides of 24 Norway spruce trees with 220 field assessments made between 1986 and 1995. The slides had been randomly arranged and assessed by three experts without knowledge of the tree number or the year when the slide was taken. Defoliation scores were computed with CROCO. Each tree had thus three different defoliation scores, field assessments, photo assessments and CROCO scores.CROCO scores were less correlated with the field assessments (Spearmans rank correlation: 0.67) than were the slide assessments with the field assessments (0.79–0.83). However, CROCO was not biased against the field scores, while slide assessments systematically underestimated defoliation.In a multi-variate mixed effect model none of the variables tree overlap, tree visibility and light conditions was significant in explaining differences between slide assessors and CROCO scores. The same model applied for the differences from the field scores yielded significant effects for poor light conditions (CROCO and all assessors), for crown overlap (CROCO and one assessor) and for visibility (one assessor). We conclude, therefore, that CROCO can be used to detect past and future changes in assessment methods without bias if poor quality photographs are avoided.     

Comparison of two reactor concepts for anoxygenic H2 production by Rhodobacter capsulatus

In the contribution at hand two main concepts of photobioreactors are considered: the flat-panel reactor and the tubular reactor. Both reactors are investigated under outdoor conditions in Germany in the summer time for their applicability for H₂ production by means of purple non-sulphur bacteria Rhodobacter capsulatus. The experiments are performed as fed batch. The performance of a photobioreactor is evaluated in terms of H₂ productivity per IRS (illuminated reactor surface). It is demonstrated that both reactors could be operated stable for several weeks with comparable H₂ productivities. The mean hydrogen productivity for the panel and the tubular reactor is 3690 ml H₂/(m_IRS² d) and 3350 ml H₂/(m_IRS² d) respectively. While approximately 8 m² of the illuminated reactor surface of the panel reactor can be installed on 1 m² of ground space, the relation of the illuminated reactor surface to the ground area for the tubular reactor is approximately 1–1.     

Norwegian farmers ceasing certified organic production: Characteristics and reasons

This article examines the characteristics of and reasons for Norwegian farmers’ ceasing or planning to cease certified organic production. We gathered cross-sectional survey data in late 2007 from organic farmers deregistering between January 2004 and September 2007 (n = 220), and similar data from a random sample of farmers with certified organic management in 2006 (n = 407). Of the respondents deregistering by November 2007, 17% had quit farming altogether, 61% now farmed conventionally, and 21% were still farming by organic principles, but without certification. Nearly one in four organic farmers in 2007 indicated that they planned to cease certification within the next 5–10 years. From the two survey samples, we categorised farmers who expect to be deregistered in 5–10 years into three groups: conventional practices (n = 139), continuing to farm using organic principles (uncertified organic deregistrants, n = 105), and stopped farming (n = 33). Of the numerous differences among these groups, two were most striking: the superior sales of uncertified organic deregistrants through consumer-direct marketing and the lowest shares of organic land among conventional deregistrants. We summarised a large number of reasons for deregistering into five factors through factor analysis: economics, regulations, knowledge-exchange, production, and market access. Items relating to economics and regulations were the primary reasons offered for opting out. The regression analysis showed that the various factors were associated with several explanatory variables. Regulations, for example, figured more highly among livestock farmers than crop farmers. The economic factor strongly reflected just a few years of organic management. Policy recommendations for reducing the number of dropouts are to focus on economics, environmental attitudes, and the regulatory issues surrounding certified organic production.     

Errors and uncertainties in a gridded carbon dioxide emissions inventory

Emission inventories (EIs) are the fundamental tool to monitor compliance with greenhouse gas (GHG) emissions and emission reduction commitments. Inventory accounting guidelines provide the best practices to help EI compilers across different countries and regions make comparable, national emission estimates regardless of differences in data availability. However, there are a variety of sources of error and uncertainty that originate beyond what the inventory guidelines can define. Spatially explicit EIs, which are a key product for atmospheric modeling applications, are often developed for research purposes and there are no specific guidelines to achieve spatial emission estimates. The errors and uncertainties associated with the spatial estimates are unique to the approaches employed and are often difficult to assess. This study compares the global, high-resolution (1 km), fossil fuel, carbon dioxide (CO₂), gridded EI Open-source Data Inventory for Anthropogenic CO₂ (ODIAC) with the multi-resolution, spatially explicit bottom-up EI geoinformation technologies, spatio-temporal approaches, and full carbon account for improving the accuracy of GHG inventories (GESAPU) over the domain of Poland. By taking full advantage of the data granularity that bottom-up EI offers, this study characterized the potential biases in spatial disaggregation by emission sector (point and non-point emissions) across different scales (national, subnational/regional, and urban policy-relevant scales) and identified the root causes. While two EIs are in agreement in total and sectoral emissions (2.2% for the total emissions), the emission spatial patterns showed large differences (10~100% relative differences at 1 km) especially at the urban-rural transitioning areas (90–100%). We however found that the agreement of emissions over urban areas is surprisingly good compared with the estimates previously reported for US cities. This paper also discusses the use of spatially explicit EIs for climate mitigation applications beyond the common use in atmospheric modeling. We conclude with a discussion of current and future challenges of EIs in support of successful implementation of GHG emission monitoring and mitigation activity under the Paris Climate Agreement from the United Nations Framework Convention on Climate Change (UNFCCC) 21st Conference of the Parties (COP21). We highlight the importance of capacity building for EI development and coordinated research efforts of EI, atmospheric observations, and modeling to overcome the challenges.