Genotoxicity evaluation of drinking water sources in human peripheral blood lymphocytes using the comet assay

The potential harm of organic pollutants in drinking water to human health is widely focused on in the wodd; more and more pollutants with genotoxic substances are released into the aquatic environment. Water source samples were collected from 7 different localities of Nanjing City. The potential genotoxicity of organic extracts from drinking water sources were investigated by means of the comet assay in human peripheral lymphocytes. The results showed that all the organic extracts from all the water source samples could induce DNA damages of human peripheral blood lymphocytes at different levels. A significant difference （P 〈 0.01） was observed when compared with the solvent control, The DNA damage increased with the increase of the dosage of the original water source. Significant differences of DNA damage were observed in different drinking water sources, as shown by the multiple comparisons analysis at the dosage of 100x; the degree of DNA damage treated by Hushu waterworks （at town level） was the most serious, the arbitrary units （AU） was 141.62±6.96, however, that of Shangyuanmen waterworks （at city level） was only 109.64±2.97. The analysis also revealed that the genotoxicity of town＇s water sources was higher than that of the city. The results demonstrated that the comet assay can be successfully applied to the genotoxicity monitoring programs of drinking water sources.     

欧美农药环境降解动力学评估方法比较

农药在环境中的降解行为是评估农药环境安全性的基础,农药的降解动力学通常以一级动力学模型描述。现有的环境暴露模型也均需要输入一级动力学模型的农药在土壤中50%消失时间(DT50)。但有时一级动力学模型不适用于描述农药在环境中,特别是土壤中的降解。因此欧盟和北美自由贸易区已发布了一系列关于使用非一级动力学模型计算DT50的导则。介绍了欧美降解动力学评估方法,并用多组降解数据比较了2种评估方法的区别。     

岩溶山地撂荒地演变研究—以郎溪槽谷区为例

中国山区面积广、坡耕地比重大,耕地撂荒不仅关系到山区农地的合理利用和可持续发展政策的制定,还对生态环境问题产生影响.因此,本文以2004年、2010年、2014年、2017年朗溪槽谷遥感影像等为数据源,通过景观格局指数、缓冲区分析及结合槽谷高程分析朗溪槽谷撂荒地分布格局、演变规律、驱动机制和撂荒效应.结果得出:耕地撂荒...     

Responses of butachlor degradation and microbial properties in a riparian soil to the cultivation of three different plants

A pot experiment was conducted to investigate the biodegradation dynamics and related microbial ecophysiological responses to butachlor addition in a riparian soil planted with different plants such as Phragmites australis, Zizania aquatica, and Acorus calamus. The results showed that there were significant differences in microbial degradation dynamics of butachlor in the rhizosphere soils among the three riparian plants. A. calamus displays a significantly higher degradation efficiency of butachlor in the rhizosphere soils, as compared with Z aquatica and P. australis. Half-life time of butachlor degradation in the rhizospheric soils of P. australis, Z aquatica, and A. calamus were 7.5, 9.8 and 5.4 days, respectively. Residual butachlor concentration in A. calamus rhizosphere soil was 35.2% and 21.7% lower than that in Z aquatica and P. australis rhizosphere soils, respectively, indicating that A. calamus showed a greater improvement effect on biodegradation of butachlor in rhizosphere soils than the other two riparian plant. In general, microbial biomass and biochemical activities in rhizosphere soils were depressed by butachlor addition, despite the riparian plant types. However, rhizospheric soil microbial ecophysiological responses to butachlor addition significantly (P < 0.05) differed between riparian plant species. Compared to Z aquatica and P. australis, A. calamus showed significantly larger microbial number, higher enzyme activities and soil respiration rates in the rhizosphere soils. The results indicated that A. calamus have a better alleviative effect on inhibition of microbial growth due to butachlor addition and can be used as a suitable riparian plant for detoxifying and remediating butachlor contamination from agricultural nonpoint pollution.     

中国生态环境监测法律制度建设实践与思考

当前,生态环保法律正在步入体系化发展阶段。搭建起环境法律制度的"四梁八柱"是法律体系的重要标志。环境监测法律制度体系作为生态环境保护法律体系的重要组成部分,发挥着重要的支撑作用。从法律制度和体系化2个维度对现有环境监测法律规范进行系统梳理和归纳分析,有利于在科学分类、质量管理、主体责任、证据效力等方面更好地完善环境监测法律制度体系。     

Feasibility assessment of up-flow anaerobic sludge blanket treatment of sulfamethoxazole pharmaceutical wastewater

The UASB system successfully treated sulfamethoxazole pharmaceutical wastewater. High concentration sulfate of this wastewater was the main refractory factor. UASB recovery performance after a few days of inflow arrest was studied. The optimal UASB operating conditions for practical application were determined. Treatment of sulfamethoxazole pharmaceutical wastewater is a big challenge. In this study, a series of anaerobic evaluation tests on pharmaceutical wastewater from different operating units was conducted to evaluate the feasibility of using anaerobic digestion, and the results indicated that the key refractory factor for anaerobic treatment of this wastewater was the high sulfate concentration. A laboratory-scale up-flow anaerobic sludge blanket (UASB) reactor was operated for 195 days to investigate the effects of the influent chemical oxygen demand (COD), organic loading rate (OLR), and COD/SO₄^2? ratio on the biodegradation of sulfamethoxazole in pharmaceutical wastewater and the process performance. The electron flow indicated that methanogenesis was still the dominant reaction although sulfidogenesis was enhanced with a stepwise decrease in the influent COD/SO₄^2? ratio. For the treated sulfamethoxazole pharmaceutical wastewater, a COD of 4983 mg/L (diluted by 50%), OLR of 2.5 kg COD/(m³·d), and COD/SO₄^2? ratio of more than 5 were suitable for practical applications. The recovery performance indicated that the system could resume operation quickly even if production was halted for a few days.     

离子色谱法同时测定大气PM2.5中水溶性有机酸和无机阴离子的研究

建立超声提取-离子色谱法55 min内同时测定大气PM_(2.5)中13种水溶性低分子有机酸和无机阴离子的方法,经不同样品膜空白值考察、预处理条件优化、样品保存实验研究等获得了最佳的实验条件。研究发现,无机阴离子线性回归系数均为0.999以上,有机酸均为0.993以上;样品膜中各离子的相对标准偏差为3.2%~9.8%,样品提取液中各离子的加标回收率为90.6%~108%;当采样体积为24 m~3时,各目标化合物的方法检出限为0.003~0.032μg/m~3。分别于2015年1、6月采集了PM_(2.5)实际样品,1月各离子浓度之和为31.9μg/m~3,范围为0.04~15.0μg/m~3;6月各离子浓度之和为22.3μg/m3,范围为0.01~12.7μg/m~3。两个采样期间,氯离子、硝酸根、硫酸根在13种化合物中占绝对优势,均占总量的95%以上;乙酸、甲酸、甲烷磺酸和乙二酸为其他低含量离子中的主要组分。     

氧化还原电位对活性污泥合成聚3-羟基丁酸-co-3-羟基戊酸酯组分的影响

分析了低DO下,乙酸钠、丙酸钠或葡萄糖作碳源,不同氧化还原电位(ORP)对活性污泥合成聚3-羟基丁酸-co-3-羟基戊酸酯(PHBV)的组分和产量的影响.结果显示,ORP从-30mV(厌氧)增加到 100mV(好氧),PHBV共聚物的产量增加,PHBV共聚物中3-羟基戊酸(HV)单体的摩尔分数降低,细胞生长量和碳源利用量增加;乙酸钠作单一碳源,ORP为-30mV时,PHBV共聚物中HV摩尔分数达到21.0%,ORP为 30mV时,PHBV共聚物在细胞内的质量分数达到35.0%.因此,DO是影响活性污泥合成PHBV共聚物的产量以及PHBV共聚物中HV摩尔分数的重要影响因素.     

城市生态文明建设评价指标体系构建

城市作为人类环境问题和危机产生的重要策源地,建立反映城市生态环境问题和生态文明建设状态的评价指标体系,依据评价结论,指导城市生态文明建设十分必要。文章在城市作为社会—经济—自然复合人工生态系统的理论基础上,分析了社会生态亚系统、经济生态亚系统及自然生态亚系统相互作用关系和城市生态系统主要生物成分组成、结构与功能关系,系统的构建了符合中国国情的城市生态文明评价指标体系。该体系以生态经济、生态环境质量、生态社会为评价要素,下设12个一级指标及42个二级指标,为评价中国城市生态文明建设水平提供科学依据。     

车辆通过交叉口的生态驾驶轨迹优化研究

车辆在道路交叉口经常出现启停和怠速现象,交叉口成为了道路交通网络中高能耗高排放的重要区域.因此需要对车辆通过交叉口的生态驾驶轨迹进行优化研究.首先将当前信号状态分为六种情景,利用数学模型对不同情景下车辆通过交叉口的驾驶轨迹进行定量表达;然后将交叉口上游和下游整体考虑,针对各情景建立了生态驾驶策略模型和生态驾驶轨迹优化算法;最后假定情景并设定参数,利用MATLAB开发程序,结合基于机动车比功率参数的排放量化模型,对各驾驶轨迹的排放进行仿真模拟.模拟结果表明:构建的生态驾驶轨迹优化算法,可使车辆CO2、NOx、CO、HC4种排放物分别降低30.1％、23.6％、24.9％、21.5％,可为面向生态的驾驶辅助系统开发提供应用算法.