环境中大肠杆菌快速生物检测技术研究进展

大肠杆菌作为水质卫生学指标,在环境水质监测中起着非常重要的指示作用.近年来为了克服大肠杆菌常规监测方法的不足,基于现代生物技术原理展开的环境中大肠杆菌的快速检测技术是国内外的研究热点.本文对环境介质大肠杆菌的分子生物学监测方法、免疫学检测方法等的原理及其在环境领域中的应用进行了综述和分析,并在此基础上对大肠杆菌的仪器化发展进行了展望.指出以现代生物技术为核心的并结合现代仪器技术的大肠杆菌快速检测技术将具有良好的应用前景,大肠杆菌的快速检测技术应向操作简单、快速、高灵敏度以及低成本方向发展.     

间接竞争ELISA方法测定水中2，4-D的研究

以2,4-D-BSA为包被抗原,采用自行制备的2,4-D单克隆特异性抗体6D11建立了水中2,4-D的间接竞争 ELISA检测方法．本研究比较了包被抗原2,4-D-BSA浓度分别为240ng·mL-1、120ng·mL-1和60ng·mL-1的反应体系和竞争反应时间为60min和15min的间接竞争ELISA剂量-反应曲线,确定了当包被抗原浓度为60 ng·mL-1、竞争反应时间为15min时,剂量-反应曲线的IC50值较低．采用上述实验条件分别测定了由PBS缓冲溶液、饮用水、清华大学地下水和圆明园福海地表水配制的2,4-D标准溶液的剂量．反应曲线,发现实际水样的基质效应对检测结果的影响较大;采用实际水样和PBS缓冲溶液配水在含有5％乙醇的PBS缓冲体系中反应的方法,基本上消除了基质效应对检测结果的干扰．采用上述优化试验条件,测定2,4-D浓度分别为0．5mg·L-1、0．125mg·L-1和0．03mg·L-1的加标样品,测定数据的准确度符合痕量有机污染物定量检测对准确度的要求,但是平行样品测定数据之间的变异系数较大,需要进一步改进检测方法,用于实际水样的检测．     

基于循环经济理念的煤炭型老工业基地振兴的研究与分析——华丰煤矿为例

华丰煤矿就其地理位置，资源条件和社会环境而言，发展循环经济有着独特优势。按照发展循环经济的要求，充分合理的开发利用资源是当前落实科学发展观所迫切需要解决的问题，发展循环经济需要市场机制与煤矿系统的双重作用，尤其离不开煤矿系统的推进。     

Solution method of overtopping risk model for earth dams

Hydrologic risk analysis relies on a series of probabilistic analyses, and it is a complex problem in estimating the probability distributions of multiple independent and random variables. The goal of this study is to presents the procedure and application of a probability-based risk analysis methodology to evaluate earth dam overtopping risk that induced by concurrent flood and wind. The uncertainty arising from initial water surface level, flood, wind velocity, and dam height are discussed in this research. The improved Monte Carlo simulation and mean-value first-order second-moment method are used to solve the proposed dam overtopping risk model, respectively. The nonparametric kernel density estimation method, which can better learn the complex multimodal characteristic of probability density function than that of traditional parametric estimation method, is employed to improve the probability density function of initial water surface level. The latin hypercube sampling is introduced to generate uniform random number, which improves the efficient and stability compared with simple random sampling. Afterward, an application to the Dongwushi Reservoir in China illustrates that the dam overtopping risk computed using the improved Monte Carlo simulation is lower than that using mean-value first-order second-moment method. Furthermore, the sensitivity analysis show that initial water surface level is more sensitive to overtopping risk than wind velocity.     

Occurrence and removal of six pharmaceuticals and personal care products in a wastewater treatment plant employing anaerobic/anoxic/aerobic and UV processes in Shanghai,China

The occurrence and removal of six pharmaceuticals and personal care products (PPCPs) including caffeine (CF), N, N-diethyl-meta-toluamide (DEET), carbamazepine, metoprolol, trimethoprim (TMP), and sulpiride in a municipal wastewater treatment plant (WWTP) in Shanghai, China were studied in January 2013; besides, grab samples of the influent were also taken every 6 h, to investigate the daily fluctuation of the wastewater influent. The results showed the concentrations of the investigated PPCPs ranged from 17 to 11,400 ng/L in the WWTP. A low variability of the PPCP concentrations in the wastewater influent throughout the day was observed, with the relative standard deviations less than 25 % for most samples. However, for TMP and CF, the slight daily fluctuation still reflected their consumption patterns. All the target compounds except CF and DEET, exhibited poor removal efficiencies (<40 %) by biological treatment process, probably due to the low temperature in the bioreactor, which was unfavorable for activated sludge. While for the two biodegradable PPCPs, CF, and DEET, the anaerobic and oxic tank made contributions to their removal while the anoxic tank had a negative effect to their elimination. The tertiary UV treatment removed the investigated PPCPs by 5–38 %, representing a crucial polishing step to compensate for the poor removal by the biologic treatment process in winter.     

资源节约型与环境友好型社会技术选择及其创新激励机制的比较研究

建设资源节约型、环境友好型社会要求必须强化技术创新的“选择”意识，除传统的经济效率标准外，还要以生态效率与其它社会价值为基准来选择技术发展的方向。基于此，特提出技术剖新选择的评价标准。并认为，当前生态化技术创新面临的主要障碍是缺乏恰当的激励机制，从而导致企业的长远发展以及人类社会的可持续发展与企业短期生存压力之间的矛盾难以化解。运用博弈论的方法比较了当前各种激励方式下社会整体帕累托最优的存在性问题。结果表明，单纯对生态化产品价格进行补贴并不能使企业生态化技术创新的程度达到社会帕累托最优的程度，创新投资补贴能直接降低企业生态化技术创新所面临的风险，因此具有更好的激励效果。     

中国企业实施可持续发展战略的态势分析

本文从绿色食品标志认证、环境标志产品认证、环境管理体系（ＩＳＯ１４０００）认证和推进清洁生产等四个方面分析了中国企业参与实施可持续发展战略的现状,指出了与国外工商企业的差距,并就进一步引导中国企业界的广泛参与提出了相应的对策与建议。     

Hydrolysis of Polylactic Acid (PLA) and Polycaprolactone (PCL) in Aqueous Acetonitrile Solutions: Autocatalysis

Polylactic acid (PLA) is a hydrolytically degradable aliphatic polyester. The rate of polymer hydrolysis increases with time, and that has been attributed to the high reactivity of the terminal ester and the kinetics of autocatalysis. Hydrolysis is carried out in an acetonitrile/water solution to eliminate any solid-state contributions such as diffusion and crystallinity to the degradation process. A kinetic equation is derived to describe the autocatalytic effect of the increasing carboxylic acid end-group concentration. The results of solution hydrolysis are examined and found to fit the derived equation. Hydrolysis was also carried out with polycaprolactone (PCL) in acetonitrile, where reaction kinetics were found to differ from those of PLA. The PCL polymer required external acid catalysis by the addition of HCl, whereas hydrolysis of PLA was selfcatalyzed by the carboxylic acid end-groups.     

几种常用无机混凝剂处理印染废水试验及评述

通过常用无机混凝剂ＰＦＳ、ＰＡＣ、ＦｅＳＯ４．７Ｈ２Ｏ及Ａｌ２（ＳＯ４）３．１８Ｈ２Ｏ处理印染废水试验，确定各自的最佳混凝处理条件。在最佳条件下，４种混凝剂对印染废水都有一定的处理效果。经成本等指标比较后，推荐ＰＦＳ为印染废水处理的适宜混凝剂。     

呼吸防护用品的分类及选用

本文较为系统地介绍了呼吸防护用品的分类方法、常见类别呼吸防护用品的特点和适用场合及呼吸防护用品选择、使用一般原则和注意事项，对呼吸防护用品的选用具有一定的指导意义。