Physical and Chemical Connectivity of Streams and Riparian Wetlands to Downstream Waters: A Synthesis

Streams, riparian areas, floodplains, alluvial aquifers, and downstream waters (e.g., large rivers, lakes, and oceans) are interconnected by longitudinal, lateral, and vertical fluxes of water, other materials, and energy. Collectively, these interconnected waters are called fluvial hydrosystems. Physical and chemical connectivity within fluvial hydrosystems is created by the transport of nonliving materials (e.g., water, sediment, nutrients, and contaminants) which either do or do not chemically change (chemical and physical connections, respectively). A substantial body of evidence unequivocally demonstrates physical and chemical connectivity between streams and riparian wetlands and downstream waters. Streams and riparian wetlands are structurally connected to downstream waters through the network of continuous channels and floodplain form that make these systems physically contiguous, and the very existence of these structures provides strong geomorphologic evidence for connectivity. Functional connections between streams and riparian wetlands and their downstream waters vary geographically and over time, based on proximity, relative size, environmental setting, material disparity, and intervening units. Because of the complexity and dynamic nature of connections among fluvial hydrosystem units, a complete accounting of the physical and chemical connections and their consequences to downstream waters should aggregate over multiple years to decades.     

Challenges and Opportunities for Creating Intelligent Hazard Alerts: The “FloodHippo” Prototype

Disasters evolving from hazards are a persistent and deadly occurrence in the United States. Despite this, hazard alerts have remained spatially vague, temporally imprecise, and lack actionable information. These deficiencies indicate a divide between the status quo and what is possible given modern environmental models, geographic information systems (GIS), and smartphone capabilities. This work describes an alternative, prototype system, “FloodHippo,” which integrates operational model outputs, cloud‐based GIS, and expanded communication channels to provide personal and interactive disaster alerts for floods. The precepts and methods underpinning FloodHippo apply equally to other disasters that evolve over space and time, presenting the opportunity for a more intelligent disaster response system. The development of such a system would not only minimize current shortcomings in disaster alerts but also improve resilience through individual action, along with community, academic, and federal cooperation.     

Is Abolitionism Guilty of Racism? A Reply to Cordeiro-Rodrigues

Gary Francione is an abolitionist: he maintains that we ought to abolish the institutions and practices that support the exploitation of animals. He also believes that veganism is the “moral baseline”—that is, he thinks it’s morally required of nearly everyone in the developed world, and many beyond it. Luis Cordeiro-Rodrigues claims that abolitionism is guilty of racism, albeit “racism without racists.” I contend that his arguments for this conclusion aren’t successful.     

Detection and Phylogenetic Analysis of the Hepatitis E Virus in a Canadian Swine Production Network

Viral contamination along the production chain is a significant concern in both food safety and livestock health. Pigs have been reported to act as a reservoir for zoonotic viruses, sometimes emerging ones, and epidemiological studies have shown direct links between the consumption of uncooked pork offal and cases of hepatitis caused by the hepatitis E virus (HEV) genotype 3 in humans. The presence of HEV in swine herds has been reported, but its dissemination in pork production environments is still unknown. To investigate viral contamination sources in the swine industry, 452 environment and fecal samples, including samples from livestock transportation vehicles, were collected over a period of 11 months from ten farms and one slaughterhouse that together represent a single production network. Hepatitis E virus RNA was detected by nested RT-PCR in 32 samples from both inside and outside farm buildings, on trucks, and, mostly, from fomites collected in the slaughterhouse yard, such as on a utility vehicle. Phylogenetic analysis showed a wide diversity of HEV genotype 3 strains, similar to human and swine strains previously found. According to the results of this study, the movements of trucks and utility vehicles might play an important role in HEV dissemination on a slaughterhouse site and throughout an entire network.     

Somatic Coliphage Profiles of Produce and Environmental Samples from Farms in Northern México

Somatic coliphages were quantified in 459 produce and environmental samples from 11 farms in Northern Mexico to compare amounts of somatic coliphages among different types of fresh produce and environmental samples across the production steps on farms. Rinsates from cantaloupe melons, jalapeño peppers, tomatoes, and the hands of workers, soil, and water were collected during 2011–2012 at four successive steps on each farm, from the field before harvest through the packing facility, and assayed by FastPhage MPN Quanti-tray method. Cantaloupe farm samples contained more coliphages than jalapeño or tomato (p range <0.01–0.03). Across production steps, jalapeños had higher coliphage percentages before harvest than during packing (p = 0.03), while tomatoes had higher coliphage concentrations at packing than all preceding production steps (p range <0.01–0.02). These findings support the use of targeted produce-specific interventions at multiple points in the process of growing and packing produce to reduce the risk of enteric virus contamination and improve food safety during fruit and vegetable production.     

Temperature-Dependent Persistence of Human Norovirus Within Oysters (<Emphasis Type="Italic">Crassostrea virginica</Emphasis>)

This study characterizes the persistence of human norovirus in Eastern oysters (Crassostrea virginica) held at different seawater temperatures. Oysters were contaminated with human norovirus GI.1 (Norwalk strain 8FIIa) by exposing them to virus-contaminated water at 15 °C, and subsequently holding them at 7, 15, and 25 °C for up to 6 weeks. Viral RNA was extracted from oyster tissue and hemocytes and quantitated by RT-qPCR. Norovirus was detected in hemocytes and oysters held at 7 and 15 °C for 6 weeks and in hemocytes and oysters held at 25 °C for up to 2 and 4 weeks, respectively. Results confirm that NoV is quite persistent within oysters and demonstrate that cooler water temperatures extend norovirus clearance times. This study suggests a need for substantial relay times to remove norovirus from contaminated shellfish and suggests that regulatory authorities should consider the effects of water temperature after a suspected episodic norovirus-contamination event.     

Structural Organization of Baculovirus Occlusion Bodies and Protective Role of Multilayered Polyhedron Envelope Protein

Baculoviruses are the ingenious insect pathogens. Outside the host, baculovirus occlusion bodies (OB) provide stability to occlusion-derived viruses (ODV) embedded within. The OB is an organized structure, chiefly composed of proteins namely polyhedrin, polyhedron envelope protein (PEP) and P10. Currently, the structural organization of OB is poorly understood and the role of OB proteins in conferring the stability to ODV is unknown. Here we have shown that the assembly of polyhedrin unit cells into an OB is a rapid process; the PEP forms in multiple layers; the PEP layers predominantly contribute to ODV viability. Full-grown OBs (n = 36) were found to be 4.0 ± 1.0 µm in diameter and possessed a peculiar geometry of a truncated rhombic dodecahedron. The atomic force microscopy (AFM) study on the structure of OBs at different stages of growth in insect cells revealed polyhedrin assembly and thickness of PEP layers. The thickness of PEP layers at 53 h post-transfection (hpt) ranged from 56 to 80 nm. Mature PEP layers filled up approximately one third of the OB volume. The size of ODV nucleocapsid was found to be 433 ± 10 nm in length. The zeta potential and particle size distribution study of viruses revealed the protective role of PEP layers. The presence of a multilayered PEP confers a viable advantage to the baculoviruses compared to single-layered PEP. Thus, these findings may help in developing PEP layer-based biopolymers for protein-based nanodevices, nanoelectrodes and more stable biopesticides.     

Assessing agricultural systems vulnerability to climate change to inform adaptation planning: an application in Khorezm,Uzbekistan

Agriculture is one of the most vulnerable sectors to climate change. The current vulnerability assessments through traditional fragmented sectoral methods are insufficient to capture the effects on complex agricultural systems. Therefore, the traditional methods need to be replaced by integrated approaches. The objective of this study is to propose a holistic vulnerability assessment method for agricultural systems. By aggregating both agro-ecological and socio-economic information, we develop an agricultural systems vulnerability index (ASVI) which allows for (i) a classification of geographical units according to their vulnerability level, (ii) an identification of key determinants of vulnerability for each unit and (iii) an assessment of adaptation policy scenarios considering their effects on the sustainability of the analysed systems. The proposed method is applied in the Khorezm region of Uzbekistan—a representative irrigated agricultural region in the lower Amu Darya river basin. A decision support tool is used to facilitate multi-criteria decision analysis, including the computation of the index and performing sensitivity analysis of the results. The assessment for Khorezm reveals significant spatial differences of vulnerability levels due to a variation of contributing factors, e.g. natural resources, water productivity, rural-urban ratio. It reveals also that feasible land and water management policies could reduce the vulnerability in Khorezm, particularly in the districts with the poorest agro-ecological conditions. Overall, the proposed method could support national and local authorities in the identification of sustainable adaptation policies for the agriculture sector.