Introduced and cryptogenic species in Port Phillip Bay, Victoria, Australia

Port Phillip Bay (PPB) is a large (1,930 km²), temperate embayment in southern Victoria, Australia. Extensive bay-wide surveys of PPB have occurred since 1840. In 1995/1996 the Commonwealth Scientific and Industrial Research Organization (CSIRO) Centre for Research on Introduced Marine Pests (CRIMP) undertook an intensive evaluation of the region with the aims of developing a comprehensive species list of native and introduced biota and contrasting previous bay-wide assessments with a current field survey in order to detect new incursions and discern alterations to native communities. Two methods were used to meet these aims: a re-evaluation of regional museum collections and published research in PPB to identify and determine the timing of introductions; and field surveys for benthic (infauna, epifauna and encrusting) organisms between September 1995 to March 1996. One hundred and sixty introduced (99) and cryptogenic (61) species were identified representing over 13% of the recorded species of PPB. As expected, the majority of these are concentrated around the shipping ports of Geelong and Melbourne. Invasions within PPB appear to be increasing, possibly due to an increase in modern shipping traffic and an increase in aquaculture (historically associated with incidental introductions); however the records of extensive biological surveys suggest that this may, in part, be an artefact of sampling effort. In contrast to Northern Hemisphere studies, PPB (and Southern Hemisphere introductions in general) have significantly different suites of successfully invading taxa. PPB is presented as one of the most invaded marine ecosystems in the Southern Hemisphere.Communicated by M.S. Johnson, Crawley     

Effect of deuterium-depleted water on reproduction of rainbow trout

During the artificial reproduction of salmonides, the fecundity can be increased either by improving the viability of spermatozoa, or by extending the time period during which a roe micropile remains open, thus allowing its fecundation. Practically, this can be achieved by the use of some fertilising techniques suitable for fish species. Here, we show that the reproduction of rainbow trout in a 1:1 solution of deuterium-depleted water and distilled water led to a significant increase in survival of roes during their embryonic development. Moreover, the addition of glucose and fructose into the deuterium-depleted fertilising solutions led to a further increase in roe survival during embryonic development. The increase in survival is mainly explained by an increase in the motility of spermatozoa.     

Toxicity of uranium mine receiving waters to early life stage fathead minnows (Pimephales promelas) in the laboratory.

Elevated concentrations of arsenic, nickel, and molybdenum in aquatic systems around northern Saskatchewan uranium mines are an environmental concern. Early life stage fathead minnows were used to assess toxicity from several aquatic systems near the Key Lake and Rabbit Lake uranium operations. Hatching success of fish embryos exposed to waters receiving contaminants associated with uranium ore milling was reduced by 32-61% relative to controls. Mortality differed in two lakes receiving mill effluents because of opposing factors influencing metal toxicity (i.e. low pH and high hardness). In one mill receiving water (Fox Lake), larval mortality was 0%, whereas mortality was 85% in water collected from a downstream location (Unknown Lake). Fish embryos exposed to open-pit dewatering effluent receiving waters, or water from a flooded open pit (i.e. pit waters), hatched 26-39% earlier than those exposed to reference or control water. The combination of low water hardness and elevated nickel concentrations in pit waters contributed to the early hatching. Egg hatchability and hatching time were more sensitive indicators of toxicity than 'standard' endpoints, like larval mortality and growth. Current regulatory emphasis on single contaminants and standard toxicological endpoints should be re-evaluated in light of the complex interaction among confounding variables such as pH, hardness. conductivity, and multi-metal mixtures.     

Genetic Diversity of Lepilemur mustelinus ruficaudatus, a Nocturnal Lemur of Madagascar

The genetic polymorphism of natural populations of Lepilemur mustelinus ruficaudatus was studied by protein electrophoresis. We sampled blood from 72 individuals from four populations separated by geographic or anthropogenic barriers from southwestern Madagascar. Six out of 22 enzyme loci showed genetic variation with a degree of polymorphism of 0.273. The expected and observed degree of genetic heterozygosity over all loci is similar to that of other primates (H_e = 0.058, H_o = 0.036). The F-statistics revealed that the four subpopulations were similar with respect to gene structure (F_ST = 0.065, p = 0.016), but the genotypic structures within subpopulations were inconsistent with random mating. For the total of the four subpopulations the proportion of heterozygous individuals was significantly smaller than expected under random mating (F_IS = 0.373, F_IT = 0.414, p < 0.01). These results correspond closely to what is expected considering the low migration ability of individuals of L. m ruficaudatus leading to small and rather isolated inbred populations.     

Effects of two organophosphorus insecticides on some biochemical constituents in the nervous system and liver of rabbits

Male Baladi rabbits were acutely and sub-chronically intoxicated with cyanofenphos and profenophos. The levels of cholesterol, triglycerides, B-lipoproteins and total proteins were determined in the serum, brain, spinal cord and sciatic nerve of rabbits. Moreover, the activities of alkaline phosphatase, acid phosphatase and glutamic-pyruvic transaminase were determined in the liver of the animals. The whole studies revealed that the biochemical constituents were highly affected by the tested insecticides. Also, the liver function suffered from adverse effects of the tested insecticides.     

Coat color polymorphism in populations of the northern mole vole in the Ural Region

Collections made in the course of long-term field studies on ecology of the northern mole vole Ellobius talpinus Pall. in the Ural Region and neighboring areas (more than 2000 individuals from 24 points of the species range) were used to analyze geographic variation in its coat color (color morphs). On the basis of long-term observations (1985–1999) on marked animals from a polymorphic population (Kurtamyshskii raion, Kurgan oblast), the life spans of males and females and the dependence of life span on population density and structure were estimated in animals of different color morphs. Each color morph of E. talpinus was shown to have specific features of the seasonal dynamics of age structure and migrations.     

Importance of eating habits and sample size in the estimation of environmental mercury contamination using biological indicators

The importance of eating habits and the number of fish sampled in the estimation of mercury environmental contamination through biological indicators was studied. The species used were one with piscivorous habits (Anguilla anguilla) and another with omnivorous habits (Ciprinus carpio). From one original sample for each species, randomized samples were generated using the subroutine GGSRS from the mathematics library IMSL, and later the statistical significance of Pearson's correlation coefficient for the relationship between fish weight and mercury concentrations in muscle was obtained for each random sample. The results show that the use of omnivorous species such as Ciprinus carpio as a biological indicator of contamination enables us to carry out estimations with a greater degree of confidence than when piscivorous species such as Anguilla anguilla are used.