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81.
In view of their possible involvement in ammonium assimilation in the coral/algal symbiosis, we have purified two distinct glutamate dehydrogenase isoenzymes from the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal) extracted from the staghorn coral Acropora formosa collected from Magnetic Island, North Queensland, Australia, in 1986–1987. An NADPH-specific glutamate dehydrogenase (GDH) displayed biphasic kinetics with respect to ammonium as the variable substrate; at low substrate concentrations the apparent K
m was below 1 mM, whereas at high substrate concentrations the corresponding value was approximately 200 mM. The NADPH-GDH displayed extremely low activity in the direction of glutamate oxidation; together with the kinetic data this suggests a probable role in ammonium assimilation. A second (NADH-specific) GDH was found to have both amination and deamination activities, and presumably functions in vivo in glutamate oxidation. Kinetic constants are reported for both GDH isoenzymes. 相似文献
82.
A Konoplev S Kaminski E Klemt I Konopleva R Miller G Zibold 《Journal of environmental radioactivity》2002,58(1):1-11
The methodology for estimating radiocaesium distribution between solid and liquid phases in lakes is applied for three prealpine lakes: Lake Constance (Germany), Lake Lugano (Switzerland) and Lake Vorsee (Germany). It is based on use of the exchangeable distribution coefficient and application of the exchangeable radiocaesium interception potential (RIPex). The methodology was tested against experimental data. Good agreement was found between estimated and measured 137Cs concentrations in Lake Constance and Lake Lugano, whereas for Lake Vorsee a discrepancy was found. Bottom sediments in Lake Vorsee are composed mainly of organic material and probably cannot be described in terms of the specific sorption characteristics attributed to illitic clay minerals. 相似文献
83.
H. F. Frolander C. B. Miller M. J. Flynn S. C. Myers S. T. Zimmerman 《Marine Biology》1973,21(4):277-288
84.
Most prebiotic syntheses depend on the reaction of concentrated precursor compounds to produce bio-organic molecules. It is now believed that the early Earth’s atmosphere was not reducing enough to have permitted copious synthesis of precursor molecules. Freezing allows reaction to occur even from dilute solution. This reaction has been demonstrated for the purines but not for the pyrimidines. It is shown here that dilute solutions of simple prebiotic molecules produce the biological pyrimidines cytosine and uracil upon freezing. Cold environments may have allowed synthesis of all of the RNA bases even from low organic yielding atmospheres, such as those of the early Earth, Mars, Titan and Europa. 相似文献
85.
Risk-based regulation assumes that risk assessment can evaluate risks against policy objectives. However, policy goals are often ambiguous and require risk assessors to interpret them for use in risk assessment. This risk assessment-policy gap stems partly from normative and imprecise policy language but is rooted more fundamentally in society's uncertain expectations for the environment. Until this uncertainty is resolved, the democratic and regulatory effectiveness of risk regulation will be undermined by ad hoc policy decisions abdicated to risk assessors. 相似文献
86.
87.
We sampled understory hummingbirds in Amazonian forest fragments from before isolation through nine years after isolation. We recorded 377 captures of eight species in five 1-ha fragments and four 10-ha fragments. The three species netted before isolation, Phaethornis superciliosus, Phaethornis bourcieri , and Thalurania furcata , were nearly equally abundant at that time. After isolation abundance of P. bourcieri and T. furcata did not change, but P. superciliosus became nearly twice as common. Five additional species that were netted only after isolation represented about 10% of the post-isolation sample. The species recorded only after isolation were forest species usually found above the levels of nets; fragments were not colonized by nonforest species. Use of fragments did not differ between 1- and 10-ha fragments. The landscape surrounding the fragments included active cattle pasture, abandoned pasture, and Cecropia -dominated second growth, but this variation had little effect on use of fragments by hummingbirds. The results suggest that these understory hummingbirds can persist in a matrix of fragments, secondary growth, and large forest patches. This response is much different than that of the insectivorous birds that dominate the understory bird community at the site, which are much more vulnerable to fragmentation. 相似文献
88.
The morphologically variable reef coral previously known as Montastraea annularis (Ellis and Solander, 1786) has recently been separated into three species based on differences in morphology, behavior, allele
frequencies and some life-history traits of Panamanian specimens. To further investigate the proposed reclassification and
its conformity to the biological species concept we conducted reciprocal intra- and inter- specific fertilization experiments
with gametes from each of the three species on Florida reefs. With one exception, self-fertilization rates were very low or
zero. Within-species crosses resulted in production of planulae, as did all inter-species (hybrid) crosses, but there was
much variation in fertilization success within each type of cross. In an experiment with separated gametes, hybrid crosses
between M. annularis and M. franksi produced more larvae than within-species crosses for each species. Hybridization crosses between M. faveolata and the other two species produced fewer larvae than did within- M. faveolata crosses in the experiment with separated gametes, but many larvae resulted when the hybridizations were performed by mixing
entire gamete bundles. Additional observations showed that M. franksi had 20% larger eggs and fewer eggs per gamete bundle than did the other two species and that it consistently spawned 1 to
1.5 h before the others, a potential temporal barrier to hybridization. These results indicate that there is no inherent pre-zygotic
barrier to cross-fertilization among the three morphological species, although post-zygotic survival and fertility remain
to be determined. The adherence of the proposed reclassification to the biological species concept requires further examination.
Received: 16 April 1997 / Accepted: 17 June 1997 相似文献
89.
Michael J. Miller Sam Wouthuyzen Gen Minagawa Jun Aoyama Katsumi Tsukamoto 《Marine Biology》2006,148(5):1101-1111
A survey for leptocephali around Sulawesi Island in the central Indonesian Seas during May 2001 found that the leptocephali
of the congrid eel, Ariosoma scheelei, were present in all seven areas that were sampled. A total of 551 leptocephali (22–166 mm TL) were collected, and A. scheelei was by far the most abundant species of leptocephali collected during the survey. The wide range of sizes in most areas indicated
that spawning had occurred during a period of several months in many different areas, although the exact spawning locations
were not determined. The larger size classes were more abundant in all areas except in Tomini Bay on the northeast side of
Sulawesi Island. The highest catch rates were observed at the eastern edge of the Java Sea and to the north in the Celebes
Sea near Makassar Strait. Premetamorphic leptocephali were also collected in surface samples at 11 stations (N=62), but metamorphosing leptocephali (N=86) were only caught in IKMT tows that fished from the surface to about 200 m. Metamorphosing leptocephali were collected
primarily at two stations in the Java Sea and Makassar Strait where a surface layer of lower-salinity water was detected.
Their total lengths (105.3–153.3 mm) and the largest premetamorphic individuals suggested that this species can reach maximum
sizes of about 165 mm before beginning to metamorphose. It is hypothesized that this species may be abundant in the Indonesian
Seas region and that it has ecological traits such as large size at recruitment and a small size at reproduction that have
made it successful in many regions of the Indo-Pacific. 相似文献
90.
T.?WernbergEmail author M.?Coleman A.?Fairhead S.?Miller M.?Thomsen 《Marine Biology》2003,143(1):47-55
Ecklonia radiata (C. Ag.) J. Agardh is a common macroalga on reefs in the warm-temperate parts of the southern hemisphere. It is a dominant habitat-former and as such has a strong structuring effect on associated algal assemblages. Morphological variation in E. radiata potentially affects its interactions with the surroundings and contributes to confusion about its taxonomy. We quantified the magnitude of morphological variation in fully developed E. radiata sporophytes across Australasia and tested the hypotheses that E. radiata has different morphology at different locations and that the degree of morphological difference depends on spatial distances among locations. A total of 11 morphological characters were sampled from 11 locations along the Australian coastline from Kalbarri in Western Australia to Sydney in New South Wales as well as from Doubtful Sound, New Zealand. Most morphological characters varied considerably from one location to another. For example, the average (±SE) thallus length was 135.2±12.5 cm in Kalbarri and only 69.7±5.5 cm in Sydney. There were no consistent spatial patterns of variation among individual morphological characters, and, generally, variations among individual characters were poorly correlated (-0.5< R<0.5). This suggests that individual morphological characters develop independently of each other in response to processes operating at different spatial scales. Multivariate measures of morphology were found to be different among some locations and similar among others (-0.37=Clarke's R=1), but there was no correlation (Spearman's R=0.08) between morphological similarity and distance between locations. Consequently, our results do not support clinal variation in E. radiata morphology. Rather, they suggest the presence of discrete morphologically different populations, in which the morphology at any one location reflects multiple forcing factors operating on different morphological characters at different spatial scales. 相似文献