Spondyloarthropathy in vertebrae of the aquatic Cretaceous snake <Emphasis Type="Italic">Lunaophis aquaticus</Emphasis>, and its first recognition in modern snakes

Inflammatory arthritis is documented for the first time in snakes. Ossification of the intervertebral capsule and zygapophyseal joints resulting in segmental vertebral fusion was observed in the aquatic Cretaceous snake Lunaophis aquaticus. Such pathologic alterations are pathognomonic for the spondyloarthropathy form of inflammatory arthritis. A survey of 2144 snakes in recent collections, performed to identify Holocene prevalence, revealed only two occurrences in extant snakes. The findings in Bitis gabonica and Elaphe taeniura were indistinguishable from those noted in Lunaophis aquaticus and identical to those previously recognized in modern varanids. The pathology likely represents a form of reactive arthritis related to enteropathic infection. While the disease probably did not affect general locomotion, its vertebral column position may have compromised mating.     

Assessing biodiversity vulnerability to climate change: testing different methodologies for Portuguese herpetofauna

Assessing biodiversity vulnerability to future climate change is essential for developing robust adaptation strategies. A number of vulnerability assessment methodologies have been developed, from bioclimatic envelop models to more complex approaches that also consider biological traits and population status. However, the lack of comparative studies leaves the user to choose among the different methodologies without much guidance. This study applied three vulnerability assessment approaches to the Portuguese herpetofauna: (I) impact assessment approach based on bioclimatic models; (II) integrated vulnerability assessment approach, adding the evaluation of adaptive capacity to approach I; and (III) integrated vulnerability assessment and validation based on expert consultation. Results showed disagreement between the different approaches for 19 % of the species studied. Most differences were found between approach III and the two other approaches. All approaches showed advantages and limitations, the choice of a methodology being ultimately dependent on the study goals. Approach I has proven efficient to capture general vulnerability patterns. Approach II, although presenting results similar to approach I, allows for the identification of key factors affecting the species adaptive capacity and may be useful in tailoring adaptation measures. Approach III further allows us to identify knowledge gaps and to evaluate vulnerability when data availability or quality is reduced. Further, because this approach is based on an expert workshop, it has proven a perfect means to build on the vulnerability assessment results to identify indicator species and prioritize specific adaptation options.     

Direct and efficient liquefaction of potato peel into bio-oil

The world has become dependent on petroleum as a feedstock for most products that we use in our daily lives. As an alternative, bio-refineries that transform biomass into biofuels and primary chemicals are emerging. Actually, many wastes are not fully recycled. For instance, potato peel is a cheap residue available from the potato industry. Up to now, potato peel has been used mostly for livestock feeding. Here, potato peel waste was subjected to direct acid liquefaction at 160 °C in 2-ethylhexanol and diethylene glycol using p-toluene sulfonic acid as catalyst. The product was characterized by infrared spectroscopy, chemical analysis, hydroxyl number and acid value. Results show that liquefaction was achieved in yields higher than 80%. The reactional profile shows two time zones, one comprising the process of liquefaction and one corresponding to the process of decomposition. Kinetics indicate that the liquefaction process occurs 1.9 times faster than the decomposition process. Overall, our findings show for the first time that, despite the water content, potato peel waste can be used to produce bio-oil. The products can be further used as fuels or refined chemicals such as levulinic acid, furfural, 5-hydroxyfurfural, dimethyl furfural and sugars in fermentation processes.     

Using shell middens to assess effects of fishing on queen conch ( Strombus gigas ) populations in Los Roques Archipelago National Park, Venezuela

Queen conch, Strombus gigas, is a commercially important gastropod that has been exploited throughout the Caribbean islands for thousands of years. Shell middens in the region are the physical record of a long-term fishery and their study can provide valuable information on selectivity patterns followed by fishermen and on resulting morphological shifts reflected by shells. In this study, we surveyed 27 middens located at Los Roques, Venezuela, to assess pre-Columbian and modern fisheries and measure their impact on local populations of queen conch. Pre-Columbian middens, covering a period of approximately 350 years of exploitation (1160–1540 A.D.), were mostly composed of adult shells (89%) and mean length of catch was estimated at 22.4 ± 0.2 cm (mean ± SE). A decrease in mean length of catch was observed throughout the modern fishery regime, estimated at 22.2 ± 0.3 cm in 1950–1971 and 20.0 ± 0.3 cm in 1990–1995. Higher proportions of immature individuals ranging from 48 to 67% were found in modern middens. Additionally, a decrease in mean length of mature individuals was detected throughout the modern fishery regime. The appearance of younger and smaller specimens is considered as a sign of heavy exploitation of local populations of queen conch during modern fisheries. Pre-Columbian middens in contrast, permitted to establish a relative baseline from which to compare values registered throughout modern times.     

Kill before being killed: an experimental approach supports the predator-removal hypothesis as a determinant of intraguild predation in top predators

Intraguild predation (IGP) has been explained in terms of competitor-removal, food-stress and predator-removal hypotheses. Only the first two hypotheses have been fairly well studied. To test the predator-removal hypothesis as a force determining IGP in avian predators, we performed a field experiment to simulate the presence of an IG predator (eagle owl Bubo bubo dummy) in the surrounding of the nests of four potential IG prey (black kite Milvus migrans, red kite Milvus milvus, booted eagle Aquila pennata and common buzzard Buteo buteo). To discard the possibility that an aggressive reaction towards the eagle owl was not related to the presence of the IG predator, we also presented a stuffed tawny owl Strix aluco, which is a potential competitor but cannot be considered an IG predator of the studied diurnal raptors considered in the experiment. While almost always ignoring the tawny owl, raptors chiefly showed an interspecific aggressive behaviour towards their IG predator. Our results seem to support the predator-removal hypothesis, as the IG prey may take advantage of the diurnal inactivity of the IG predator to remove it from their territory. However, the recorded behaviour may be also considered as a special variety of mobbing (i.e. a prey’s counter-strategy against its predator), where the mobber is sufficiently powerful to escalate predator harassment into deliberate killing attempts. In their turn, eagle owls can respond with an IG predatory behaviour aimed at removing IG prey species which are highly aggressive mobbers.     

Impacts of changes in land use and fragmentation patterns on Atlantic coastal forests in northern Spain

Changes in forested landscapes may have important consequences for ecosystem services and biodiversity conservation. In northern Spain, major changes in land use occurred during the second half of the 20th century, but their impacts on forests have not been quantified. We evaluated the dynamics of landscape and forest distribution patterns between 1957 and 2003 in Fragas do Eume Natural Park (northwestern Spain). We used orthoimages and a set of standard landscape metrics to determine transitions between land cover classes and to examine forest distribution patterns. Eucalypt plantations showed the greatest increase in area (197%) over time. Furthermore, transitions to eucalypt plantations were found in all major land cover classes. Forest showed a net decline of 20% in total area and represented 30% of the landscape area in 2003. Forest losses were mainly due to eucalypt plantations and the building of a water reservoir, while forest gains were due to increases in shrubland, meadows and cultivated fields which had been recolonised. Forest patch size and core area decreased, and edge length increased over time. In turn, increases were obtained in mean distance between forest patches, and in adjacency to eucalypt plantations and to a water reservoir. These results suggest an increase in forest fragmentation from 1957 to 2003, as well as a change in the nature of the habitat surrounding forest patches. This study shows that land use changes, mostly from eucalypt plantation intensification, negatively affected forested habitats, although some regeneration was ongoing through ecological succession from land abandonment.     

Variation of surface ozone in Campo Grande,Brazil: meteorological effect analysis and prediction

The effect of meteorological variables on surface ozone (O₃) concentrations was analysed based on temporal variation of linear correlation and artificial neural network (ANN) models defined by genetic algorithms (GAs). ANN models were also used to predict the daily average concentration of this air pollutant in Campo Grande, Brazil. Three methodologies were applied using GAs, two of them considering threshold models. In these models, the variables selected to define different regimes were daily average O₃ concentration, relative humidity and solar radiation. The threshold model that considers two O₃ regimes was the one that correctly describes the effect of important meteorological variables in O₃ behaviour, presenting also a good predictive performance. Solar radiation, relative humidity and rainfall were considered significant for both O₃ regimes; however, wind speed (dispersion effect) was only significant for high concentrations. According to this model, high O₃ concentrations corresponded to high solar radiation, low relative humidity and wind speed. This model showed to be a powerful tool to interpret the O₃ behaviour, being useful to define policy strategies for human health protection regarding air pollution.     

Identification of best available techniques in the seafood industry: a case study

A Best Available Techniques (BAT) analysis of a mussel canning processing facility in northwest Spain was undertaken to investigate potential cleaner production and efficiency improvements. Potential techniques were identified from the literature and from our experience in the seafood sector. BATs were then assessed for possible implementation in a mussel canning facility. This work describes, in considerable detail, the advantages and disadvantages of the techniques discussed.     

Enhancement of conservation knowledge through increased access to botanical information

Herbarium specimens are increasingly recognized as an important resource for conservation science and virtual herbaria are making specimens freely available to a wider range of users than ever before. Few virtual herbaria are designed with conservation use as a primary driver. Exceptionally, Brazil's Reflora Virtual Herbarium (RVH) was created to increase knowledge and conservation of the Brazilian flora. The RVH is closely integrated with the Flora of Brazil 2020 platform on which Brazil's new national Flora is under construction. Both resources are accessible via the Reflora home page and thousands of users move seamlessly between these Reflora resources. To understand how the Reflora resources are currently used and their impact on conservation science, we conducted a literature review and an online survey. We searched for publications of studies in which Reflora resources were used and publications resulting from Brazilian researchers who were part of Reflora's research and mobility program. The survey contained multiple choice questions and questions that required a written response. We targeted Reflora webpage visitors with the survey to capture a wider range of Reflora users than the literature review. Reflora resources were used for a variety of conservation-relevant purposes. Half the 806 scientific publications in which Reflora was cited and 81% of the 1069 survey respondents accessing Reflora resources mentioned conservation-relevant research outputs. Most conservation-relevant uses of the Reflora resources in scientific publications were research rather than implementation focused. The survey of Reflora users showed conservation uses and impacts of virtual herbaria were more numerous and diverse than the uses captured in the literature review. Virtual herbaria are vital resources for conservation science, but they must document use and impacts more comprehensively to ensure sustainability.