A Modeling System to Assess Land Cover Land Use Change Effects on SAV Habitat in the Mobile Bay Estuary

Estuarine ecosystems are largely influenced by watersheds directly connected to them. In the Mobile Bay, Alabama watersheds we examined the effect of land cover and land use (LCLU) changes on discharge rate, water properties, and submerged aquatic vegetation, including freshwater macrophytes and seagrasses, throughout the estuary. LCLU scenarios from 1948, 1992, 2001, and 2030 were used to influence watershed and hydrodynamic models and evaluate the impact of LCLU change on shallow aquatic ecosystems. Overall, our modeling results found that LCLU changes increased freshwater flows into Mobile Bay altering temperature, salinity, and total suspended sediments (TSS). Increased urban land uses coupled with decreased agricultural/pasture lands reduced TSS in the water column. However, increased urbanization or agricultural/pasture land coupled with decreased forest land resulted in higher TSS concentrations. Higher sediment loads were usually strongly correlated with higher TSS levels, except in areas where a large extent of wetlands retained sediment discharged during rainfall events. The modeling results indicated improved water clarity in the shallow aquatic regions of Mississippi Sound and degraded water clarity in the Wolf Bay estuary. This integrated modeling approach will provide new knowledge and tools for coastal resource managers to manage shallow aquatic habitats that provide critical ecosystem services.     

Integrated Approach for Prioritizing Watersheds for Management: A Study of Lidder Catchment of Kashmir Himalayas

The Himalayan watersheds are susceptible to various forms of degradation due to their sensitive and fragile ecological disposition coupled with increasing anthropogenic disturbances. Owing to the paucity of appropriate technology and financial resources, the prioritization of watersheds has become an inevitable process for effective planning and management of natural resources. Lidder catchment constitutes a segment of the western Himalayas with an area of 1,159.38 km². The study is based on integrated analysis of remote sensing, geographic information system, field study, and socioeconomic data. Multicriteria evaluation of geophysical, land-use and land-cover (LULC) change, and socioeconomic indicators is carried out to prioritize watersheds for natural resource conservation and management. Knowledge-based weights and ranks are normalized, and weighted linear combination technique is adopted to determine final priority value. The watersheds are classified into four priority zones (very high priority, high priority, medium priority, and low priority) on the basis of quartiles of the priority value, thus indicating their ecological status in terms of degradation caused by anthropogenic disturbances. The correlation between priority ranks of individual indicators and integrated indicators is drawn. The results reveal that socioeconomic indicators are the most important drivers of LULC change and environmental degradation in the catchment. Moreover, the magnitude and intensity of anthropogenic impact is not uniform in different watersheds of Lidder catchment. Therefore, any conservation and management strategy must be formulated on the basis of watershed prioritization.     

Effect of Tinospora cordifolia on the reduction of ultraviolet radiation‐induced cytotoxicity and DNA damage in PC12 cells

The safety of Tinospora cordifolia and its potential to protect against ultraviolet radiation‐induced cytotoxicity and DNA damage in PC12 cells were investigated. To evaluate the safety of T. cordifolia, cell viability and agarose gel electrophoresis were carried out using PC12 cells treated with 0 to 100 μg mL^?1 of methanol extract of T. cordifolia. T. cordifolia extracts did not show cytotoxicity ranging 0 to 100 μg mL^?1. In addition, T. cordifolia extracts significantly increased cell viability at 1 ng, 10 ng and 1 μg mL^?1 concentrations in serum‐deprived medium compared to control. To confirm the protective role against UV‐induced damage, PC12 cells alone or in the presence of 10 ng, 100 ng, or 1 μg mL^?1 of T. cordifolia extract were exposed to 250, 270 and 290 nm of UV radiation, which corresponded to doses of 120, 150 and 300 mJ cm^?2, respectively. Treatment with T. cordifolia extracts significantly increased the cell survival rate irradiated at 290 nm. In addition, T. cordifolia extracts significantly reduced cyclobutane pyrimidine dimer formation induced by UV irradiation at all wavelengths. In conclusion, T. cordifolia is not toxic and safe for cells. Our findings can support its application as phototherapy in the medical sector.     

Sonophotocatalytic Degradation of Chitosan in the Presence of Fe(III)/H2O2 System

The degradation of chitosan by means of ultrasound irradiation and its combination with homogeneous photocatalysis (photo-Fenton) was investigated. Emphasis was given on the effect of additive on degradation rate constants. 24 kHz of ultrasound irradiation was provided by a sonicator, while an ultraviolet source of 16 W was used for UV irradiation. To increase the efficiency of degradation process, degradation system was combined with Fe(III) (2.5 × 10⁻⁴mol/L) and H₂O₂ (0.020–0.118 mol/L) in the presence of UV irradiation and the rate of degradation process change from 1.873 × 10⁻⁹−6.083 × 10⁻⁹ mol^1.7 L s⁻¹. Photo-Fenton process led to complete chitosan degradation in 60 min with the rate increasing with increasing catalyst loading. Sonophotocatalysis in the presence of Fe(III)/H₂O₂ was always faster than the respective individual processes. A synergistic effect between ultrasound and ultraviolet irradiation in the presence of Fenton reagent was calculated. The degraded chitosans were characterized by X-ray diffraction (XRD), gel permeation chromatography (GPC) and Fourier transform infrared (FT-IR) spectroscopy and average molecular weight of ultrasonicated chitosan was determined by measurements of intrinsic viscosity of samples. The results show that the total degree of deacetylation (DD) of chitosan change, partially after degradation and the decrease of molecular weight led to transformation of crystal structure. A negative order for the dependence of the reaction rate on total molar concentration of chitosan solution within the degradation process was suggested. Results of this study indicate that the presence of catalyst in the reaction medium can be utilized to reduce molecular weight of chitosan while maintaining the power of irradiated ultrasound and degree of deacetylation.     

In vivo studies on the effect of some insecticides on the hepatic activities of L-tryptophan 2,3-dioxygenase and pyridoxal phosphokinase of male mice

The effect of Dimethoate, Carbaryl and Permethrin on the activities of liver L-tryptophan 2,3-dioxygenase (total-, holo-, and apo-forms) and pyridoxal phosphokinase of male mice was investigated. Dimethoate inhibited both enzyme systems after single and repeated dose treatment; except the dioxygenase holo-enzyme after repeated doses. Single dose of Carbaryl treatment inhibited the pyridoxal phosphokinase, total and holo-enzyme of L-tryptophan dioxygenase. However, the repeated dose treatment do not affect both enzyme systems. Permethrin inhibited only total-, holo- and apo-enzymes of L-tryptophan dioxygenase whereas repeated administration has no significant effect on both enzymes. The data indicate that these insecticides may induce abnormal tryptophan metabolism through which some endogenous bladder carcinogens are formed.     

Sulfur speciation: Methodology and application to sulfide oxidation studies at the sediment-water interface

A single column ion chromatographic method for the determination of sulfide, sulfite, sulfate and thiosulfate was developed. It uses an anion exchange column (Waters, IC-Pak A) and a borate-gluconate buffer at pH 8.5 that contains EDTA and ascorbic acid to prevent sulfite oxidation. This eluent has relatively low background values for both conductivity and ultraviolet absorption, which allows determination of the above sulfur anions with high sensitivity. The mean percent recovery of the investigated anions in synthetic mixtures were 98.6, 100.0, 99.6 and 100.2 % for sulfide, sulfite, thiosulfate and sulfate, respectively. The method was applied to study sulfide ion oxidation at the water-sediment interface using six aquifer samples collected within the continental United States. Results indicated that sulfide ion disappearance follows a pseudo first-order profile and that the rate of disappearance correlates with the total organic carbon and clay content of the sediment.     

Synthesis of TiO2 nanoparticles in different thermal conditions and modeling its photocatalytic activity with artificial neural network

Titanium dioxide (TiO₂) nanoparticles were prepared by sol gel route. The preparation parameters were optimized in the removal of 4-nitrophenol (4-NP). All catalysts were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). An artificial neural network model (ANN) was developed to predict the photocatalytic removal of 4-NP in the presence of TiO₂ nanoparticles prepared under desired conditions. The comparison between the predicted results by designed ANN model and the experimental data proved that modeling of the removal process of 4-NP using artificial neural network was a precise method to predict the extent of 4-NP removal under different conditions.     

Aminobenzenesulfonamide functionalized SBA-15 nanoporous molecular sieve: A new and promising adsorbent for preconcentration of lead and copper ions

A rapid method for the extraction and monitoring of nanogram level of Pb²⁺ and Cu²⁺ ions using uniform silanized mesopor (SBA-15) functionalized with aminobenzenesulfonamide groups and flame atomic absorption spectrometry (FAAS) is presented. Aminobenzenesulfonamide functionalized SBA-15 was synthesized according to procedure in the literature and the presence of organic groups in the silica framework was demonstrated by FT-IR spectra. The functionalized product showed the BET surface area 110 m²/g and pore diameter 5.1 nm, based on adsorption-desorption of N₂ at 77 K. The effect of several variables such as (amount of adsorbent, stirring time, pH and presence of other ions in the medium) has been studied. Lead and copper were completely extracted at pH greater than 3 after stirring for 10 min. The maximum capacity of the adsorbent was found to be 191.3 ± 1.4 and 155.0 ± 1.0 μg of lead and copper ions/mg functionalized SBA-15, respectively. The preconcentration factor of the method was found to be 200. The detection limit of the technique was 3.4 and 0.4 ng/mL for Pb²⁺ and Cu²⁺, respectively. The applications of this methodology for real samples were examined by various water type, black tea and pepper samples.     

Acute and chronic toxicity of nickel to nitrifiers at different temperatures

This study investigated the acute nickel toxicity on nitrification of low ammonia synthetic wastewater at 10, 23, and 35°C. The nickel inhibition half-velocity constants(K_(I,Ni)) for ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria(NOB) based on Ni/MLSS ratio at 10, 23, and 35°C were 5.4 and 5.6 mg Ni/g MLSS, 4.6 and 3.5 mg Ni/g MLSS, and 9.1 and 2.7 mg Ni/g MLSS, respectively. In addition, chronic toxicity of nickel to nitrification of low ammonia synthetic wastewater was investigated at 10°C in two sequencing batch reactors(SBRs). Long-term SBRs operation and short-term batch tests were comparable with respect to the extent of inhibition and corresponding Ni/MLSS ratio. The μ_(max), b, and K_o of AOB were 0.16 day~(-1), 0.098 day~(-1) and 2.08 mg O_2/L after long-term acclimatization to nickel of 1 mg/L at 10°C, high dissolved oxygen(DO)(7 mg/L) and long solids retention time(SRT) of 63–70 days. Acute nickel toxicity of nitrifying bacteria was completely reversible.