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691.
E. Gornung C. Cordisco A. Rossi De S. Innocentiis D. Crosetti L. Sola 《Marine Biology》2001,139(1):55-60
This study continues a comparative cytogenetic analysis of the fish family Mugilidae, reporting the karyotype characterization of the leaping mullet, Liza saliens, by C-banding, Ag- and fluorochrome-staining, and completing the fluorescence in situ hybridization (FISH)-mapping of the 18S and 5S rRNA genes (rDNA) to the chromosomes of the six Mediterranean mullets, namely L. saliens, L. ramada, L. aurata, Mugil cephalus, Chelon labrosus and Oedalechilus labeo. In all species, except M. cephalus, the 5S rDNA sites were localized on a medium-sized acrocentric chromosome pair, which was considered homeologous in all of them. In L. saliens, an additional 5S rDNA site was detected in a location close to the one shown by major ribosomal genes in M. cephalus, i.e. the subtelomeric region of chromosome pair 1. The 5S rDNA site in M. cephalus is located on the smallest chromosome pair of the complement, which, on the other hand, though on a different position, bears 18S rDNA in all the species of Liza and Chelon examined. The heterochromatin composition and the major and minor ribosomal gene locations suggest that the karyotype of L. saliens (subgenus Protomugil) can be considered intermediate between the karyotype of the more primitive M. cephalus and those of the other Liza (subgenus Liza) species and of the representatives of the more derived genera Chelon and Oedalechilus. 相似文献
692.
Since its introduction to Nova Scotia in the early 1990s, the invasive green alga Codium fragile ssp. tomentosoides has spread rapidly to become a dominant and persistent component of seaweed assemblages in the rocky subtidal zone. To examine the potential of sea urchins (Strongylocentrotus droebachiensis) to regulate Codium, and the potential of the alga to support urchin growth and reproduction, we fed urchins Codium and/or kelp (Laminaria spp., a high quality and preferred native food) in the laboratory for 11 months. Urchins showed a strong and active preference for kelp: they consumed more Laminaria than Codium (as dry weight) in single and mixed diet treatments. Urchins fed only Codium consumed 3.5 times more of the alga than those on a mixed diet, but did not increase their feeding rate in winter when kelp consumption was greatest. Laminaria was consumed at the same rate in single and mixed diets, indicating that the presence of an alternative food had no effect on kelp consumption. Survival and growth (change in test diameter) were lowest on the Codium diet, although the differences among diets were not statistically significant. Urchins on the Codium diet showed no gonadal production over the annual reproductive cycle, compared to a marked rise in gonad index on the Laminaria and mixed diets. Our results indicate that Codium is an unattractive, unpalatable and poor quality food, which is not readily consumed by urchins. Thus we predict that urchins at moderate densities will be much more likely to graze kelp than Codium in mixed stands, creating a mosaic of Codium patches and barren areas. At high densities, however, urchins are likely to destructively graze all seaweeds, although dense stands of the invasive alga may prolong the widespread formation of barrens. 相似文献
693.
Juvenile and adult marine organisms differ in their morphology, chemistry, physiology, behavior, and ecology. Because juvenile
algae are thinner, smaller, and have more delicate tissues than adults, they are often assumed to be more susceptible to grazers.
We examined within-species food preferences of four common generalist herbivores for juvenile and adult tissues of eight common
brown algae in two-choice laboratory food-preference experiments. Our results showed that juvenile algae did not tend to be
a preferred food of herbivores. Juvenile tissues were significantly preferred over adult tissues in only four of the 32 combinations
of algae and herbivores tested. In 12 experiments, adult tissues were preferred over juvenile tissues, and no choice occurred
in the remaining 16 experiments. When sea urchins exhibited a preference, it was always for adult tissues. The other three
herbivores, an isopod and two snails, were more variable in their choices, sometimes preferring juveniles, sometimes adults,
and sometimes having no preference. We measured nitrogen and phlorotannin concentrations in adult and juvenile seaweeds to
see whether these parameters were correlated with herbivore food preferences. Nitrogen levels were similar in juveniles and
adults of three algal species and were higher in juveniles of two. Phlorotannin levels were higher in juveniles of four species
and lower in juveniles of one. The other three species showed no differences in phlorotannin levels. Phlorotannin concentrations
decreased with increasing juvenile size in three species and increased with increasing size in one species. Neither nitrogen
nor phlorotannin concentrations explained overall herbivore food preferences for algae of different stages. Our results suggest
that preferences of certain grazers for juvenile algae are not as strong as previously assumed and are dependent on herbivore
species. Preferences between juveniles and adults are likely to be determined by a combination of morphological and chemical
features of the tissues and the unique responses of herbivore species to those features.
Received: 10 April 2000 / Accepted: 19 November 2000 相似文献
694.
M. Böhm U. Hentschel A. Friedrich L. Fieseler R. Steffen V. Gamulin I. Müller W. Müller 《Marine Biology》2001,139(6):1037-1045
The aim of this study was the documentation of the molecular immune response of Suberites domuncula upon bacterial infection. Additionally, the bacteria that are naturally present in the sponge after prolonged aquarium maintenance were characterized. After 6 months of maintenance of S. domuncula in seawater aquaria, only one bacterial 16S rDNA sequence could be recovered, which belongs to the genus Pseudomonas. Concomitantly, morphologically uniform bacteria were found encapsulated in bacteriocytes. These findings indicate that certain bacteria, possibly of the genus Pseudomonas, are able to persist for long periods in host bacteriocytes. Subsequent to performing a previously established infection assay with S. domuncula, a potentially pathogenic Vibrio sp. was isolated from the tissues. Furthermore, the host tissue disintegrated and asexual propagation bodies (gemmules) were formed. In order to gain insights into the molecular events occurring after bacterial infection, the stress-response kinases, p38 protein kinase and JNK protein kinase, were analyzed. It is demonstrated that these two kinases are activated (phosphorylated) upon incubation of the tissue with the bacterial endotoxin lipopolysaccharide (LPS). Moreover, LPS strongly inhibits protein synthesis. It is concluded that there are many functionally different interactions between S. domuncula and bacteria and that the animal possesses mechanisms to differentiate between bacteria and to respond accordingly. 相似文献
695.
Effects of salinity on endogenous rhythm of the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae) 总被引:1,自引:0,他引:1
The Manila clam Ruditapes philippinarum, an intertidal bivalve, was exposed to different salinity regimes (from 31.0–31.7‰ down to 20‰, 15‰, 10‰, 5‰), and the endogenous
rhythm in its oxygen consumption was studied using an automatic intermittent-flow respirometer. When exposed to salinities
reduced from 31.5‰ to 20‰ and 15‰ under otherwise constant conditions, the clams recovered a clear endogenous circatidal rhythm
in their oxygen-consumption rate after having dampened periods of 12 h and 48 h, respectively. At salinities less than 10‰,
however, the oxygen-consumption rate was depressed greatly at the beginning of the experiment for about 36 h and then increased
to a level higher than normal, but the rhythm of oxygen consumption was not recovered. The results of this study indicate
that the Manila clam, a euryhaline organism, cannot maintain a normal metabolic activity at a salinity lower than 15‰. All
clams were dead after exposure at a salinity of 5‰ for 7 days.
Received: 28 February 2000 / Accepted: 26 August 2000 相似文献
696.
697.
We determined the nuclear 18S rRNA sequences for 41 species of octocorals and used these to address the validity of the historical
ordinal divisions and the current subordinal divisions within the subclass Octocorallia. We also explored the phylogenetic
affinities of the species Dendrobrachia paucispina, which was originally classified in the order Antipatharia (subclass Ceriantipatharia) although polyp structure indicates
it belongs in the subclass Octocorallia. Trees constructed using maximum likelihood techniques are incongruent with the current
and historical taxonomy of the Octocorallia. There appeared to be three major clades of octocorals. The first clade included
most, but not all, pennatulaceans as a monophyletic group. The second clade contained 21 species, representing all major octocoral
groups other than pennatulaceans. The third clade contained members from three suborders of the Alcyonacea and one member
of the Pennatulacea. These data could not be used to distinguish the branching order of the three major clades. The species
D. paucispina had a close affinity with the genera Corallium and Paragorgia (Alcyonacea: Scleraxonia), although its morphology suggests it is more similar to the genus Chrysogorgia (Alcyonacea: Calcaxonia). The morphological character of dimorphism (the presence of both autozooids and siphonozooids within
a single colony) corresponded loosely with the topology of the most likely trees, and a single origin of dimorphism could
not be rejected. Despite sampling from the majority of families within the Octocorallia, many of the relationships within
this group remain ambiguous.
Received: 16 June 2000 / Accepted: 14 September 2000 相似文献
698.
Noninvasive Stress and Reproductive Measures of Social and Ecological Pressures in Free-Ranging African Elephants 总被引:7,自引:0,他引:7
Abstract: The African elephant ( Loxodonta africana ) experienced a poaching-related 60% population decline between 1979 and 1988 that was inordinately concentrated on adults. This, coupled with political pressures to delist the elephant, has created a need for noninvasive physiological measures that can quantify the long-term effects of past mortality patterns of this long-lived species. We collected fresh fecal samples from 16 female elephants in three different groups over 23 months at Tarangire National Park, Tanzania, and analyzed them for fecal progesterone and cortisol metabolites. Social and ecological measures were collected concurrently. Fecal progesterone metabolite measures corresponded significantly with stage of gestation, and appear to be able to confirm pregnancy in female elephants from as early as 3 months of gestation. We found that progesterone metabolite concentrations were significantly lower during the dry season than during the wet season after controlling for stage of gestation. Fecal cortisol metabolite concentrations showed the opposite seasonal pattern, being significantly higher in the dry season and inversely correlated with rainfall across seasons. Fecal cortisol metabolite concentrations also increased with group size and were correlated positively with dominance rank in the largest group. Our results suggest that measures of progesterone and cortisol metabolites in feces provide indices of reproductive function and physiological stress that can quantify both natural and human disturbances in African elephants. These measures are ideally suited for monitoring the long-term effects of social disruption from poaching and a variety of other management concerns. 相似文献
699.
A Systematic Test of an Enterprise Strategy for Community-Based Biodiversity Conservation 总被引:6,自引:0,他引:6
N. Salafsky H. Cauley G. Balachander B. Cordes J. Parks C. Margoluis S. Bhatt C. Encarnacion D. Russell R. Margoluis 《Conservation biology》2001,15(6):1585-1595
Abstract: A commonly held belief is that if people can benefit financially from enterprises that depend on nearby forests, reefs, and other natural habitats, then they will take action to conserve and sustainably use them. The Biodiversity Conservation Network brought together conservation and development organizations and local communities to systematically test this hypothesis across 39 conservation project sites in Asia and the Pacific. Each project implemented one or more community-based enterprises such as setting up an ecotourism lodge, distilling essential oils from wild plant roots, producing jams and jellies from forest fruits, harvesting timber, or collecting marine samples to test for pharmaceutical compounds. Each project team collected the biological, enterprise, and social data necessary to test the network's hypothesis. We present the results of this test. We found that a community-based enterprise strategy can lead to conservation, but only under limited conditions and never on its own. We summarize the specific conditions under which an enterprise strategy will and will not work in a decision chart that can be used by project managers to determine whether this strategy might make sense at their site. We also found that an enterprise strategy can be subsidized and still create a net gain that pays for conservation. Based on our experiences, we recommend developing "learning portfolios" that combine action and research to test other conservation strategies. 相似文献
700.