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741.
742.
743.
One-day-old anarchistic (selected for successful worker reproduction) and wild-type honey-bee workers were introduced into queenright colonies of honey-bees of two treatments. In treatment 1, all eggs and larvae were offspring of queens from an anarchistic line. In treatment 2, all eggs and larvae were offspring of wild-type queens. In both treatments, adult workers were wild type. This experimental arrangement was used to test the importance of larval genotype on ovary activation in young adult workers. After 12 days, the introduced bees were dissected to determine the frequency of ovary activation. In those colonies provided with wild-type brood, 0% of introduced wild-type bees and 16% of anarchistic bees had activated ovaries. In those colonies provided with anarchistic brood, 13% of introduced wild-type bees and 41% of anarchistic bees had activated ovaries. These results strongly support the hypothesis that selection for high levels of worker reproduction in anarchistic stocks has reduced the amount or composition of brood pheromones produced by larvae that normally signal workers to refrain from reproduction. They also suggest that anarchistic workers have a higher threshold for these signals than wild-type bees. 相似文献
744.
Jasmonic acid treatment and mammalian herbivory differentially affect chemical defenses and growth of wild mustard (Brassica kaber) 总被引:2,自引:0,他引:2
Summary. Jasmonic acid (JA) is a wound-related hormone found in most plants that, when applied exogenously, can induce increases in
levels of chemical defenses in patterns similar to those induced by mechanical damage or insect feeding. Relative to responses
to insect and pathogen attack, chemical responses of herbaceous plants to mammalian herbivore attack have been little studied.
In a field experiment, we compared the effects of JA treatment and naturally occurring mammalian herbivory on the expression
of trypsin inhibitors, glucosinolates, peroxidase activity and growth of wild mustard (Brassica kaber). Exogenous JA significantly increased trypsin inhibitor activity and glucosinolate concentration, and moderately increased
peroxidase activity in the eighth true leaves of five-week-old plants, relative to untreated controls. In contrast, levels
of these chemical defenses in the eighth true leaves or in regrowth foliage of plants that had ∼80% of their leaf area removed
by groundhogs (Marmota monax) did not differ from that in undamaged and untreated controls. Although exogenous JA significantly elevated levels of chemical
defenses, it did not affect height of plants through the season and only slightly reduced time to first flower. Groundhog
herbivory significantly reduced height and delayed or abolished flowering, but these effects were not substantial unless coupled
with apical meristem removal. We hypothesize that the lack of effect of groundhog herbivory on chemical defenses may be due
in part to the speed and pattern of leaf area removal by groundhogs, or physiological constraints caused by leaf area loss.
Despite having no effect on chemical defense production, leaf area loss by groundhogs was more costly to growth and fitness
than the effects of JA application in this study, but only substantially so if coupled with apical meristem removal. We suggest
that in general, costs of defense production in plants are likely to be minimal when compared to the risk of losing large
amounts of leaf area or primary meristematic tissue. Thus, if they are effective at deterring herbivory, the benefits of inducible
defense production likely outweigh the costs in most cases.
Received 20 December 2000; accepted 3 May 2001 相似文献
745.
We determined the nuclear 18S rRNA sequences for 41 species of octocorals and used these to address the validity of the historical
ordinal divisions and the current subordinal divisions within the subclass Octocorallia. We also explored the phylogenetic
affinities of the species Dendrobrachia paucispina, which was originally classified in the order Antipatharia (subclass Ceriantipatharia) although polyp structure indicates
it belongs in the subclass Octocorallia. Trees constructed using maximum likelihood techniques are incongruent with the current
and historical taxonomy of the Octocorallia. There appeared to be three major clades of octocorals. The first clade included
most, but not all, pennatulaceans as a monophyletic group. The second clade contained 21 species, representing all major octocoral
groups other than pennatulaceans. The third clade contained members from three suborders of the Alcyonacea and one member
of the Pennatulacea. These data could not be used to distinguish the branching order of the three major clades. The species
D. paucispina had a close affinity with the genera Corallium and Paragorgia (Alcyonacea: Scleraxonia), although its morphology suggests it is more similar to the genus Chrysogorgia (Alcyonacea: Calcaxonia). The morphological character of dimorphism (the presence of both autozooids and siphonozooids within
a single colony) corresponded loosely with the topology of the most likely trees, and a single origin of dimorphism could
not be rejected. Despite sampling from the majority of families within the Octocorallia, many of the relationships within
this group remain ambiguous.
Received: 16 June 2000 / Accepted: 14 September 2000 相似文献
746.
Previously published mtDNA cytochrome b sequences for Xyrichtys novacula (Linnaeus) and two morphologically distinct types of Xyrichtys larvae (Pisces: Labridae) included human cytochrome b sequence, presumably due to sample contamination and/or poor preservation of sample DNA. Those sequences had been used to
identify the two types of Xyrichtys larvae as X. novacula, but owing to the contamination, the identifications are invalid. Fresh specimens were collected: X.␣martinicensis (Cuvier and Valenciennes), X. novacula, X. splendens (Castelnau), and Xyrichtys sp. larvae of each of the two morphotypes (dorsal-forward eye and ventral-forward eye). The cytochrome b fragment was amplified from each specimen using the polymerase chain reaction. Comparison of the sequences with human cytochrome b sequence confirmed that the new sequences were not contaminated. The interspecific differences in Xyrichtys sequences were less than previously reported, but still greater than observed in many fish genera. Distance and parsimony
analyses indicated that X. novacula and X. martinicensis were more closely related to each other than to X. splendens. This conclusion differs from our previous conclusion that X. martinicensis and X. splendens were more closely related to each other than to X. novacula. Distance and parsimony analyses also demonstrated that both larval morphotypes were X. novacula and, thus, our previous conclusions regarding larval ecology and oceanographic transport remain unchanged.
Received: 12 March 1997 / Accepted: 24 October 1997 相似文献
747.
Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization 总被引:1,自引:0,他引:1
F. Fontana J. Tagliavini L. Congiu M. Lanfredi M. Chicca C. Laurente R. Rossi 《Marine Biology》1998,132(3):495-501
A karyotype analysis by several staining techniques was carried out on the great sturgeon, Huso huso (Linnaeus, 1758). The karyotype (2n = 118 ± 2) was composed of 42 pairs of meta-/submetacentric chromosomes and 17 pairs of acrocentrics/microchromosomes. Constitutive
heterochromatin was mainly located at the centromeric regions of the acrocentric chromosomes. The biarmed chromosomes showed
weak C-bands. Fluorescent staining with GC-specific chromomycin A3 showed clearly recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific
4,6-diamidino-2 phenylindole. After Ag-staining, nucleolar organizer regions could be observed on the short arms of two medium-sized
submetacentrics and on two acrocentrics. Digoxigenated 28S and 5S rDNA probes, prepared from Acipenser naccarii DNA and hybridized to metaphase chromosomes, showed signals on six and two chromosomes, respectively. The telomeric sequence
(TTAGGG)
n
detected by FISH was located at both ends of each chromosome. Results are discussed in relation to karyotype organization
and evolution in sturgeons.
Received: 1 April 1998 / Accepted: 21 July 1998 相似文献
748.
In this study, an interval-fuzzy two-stage chance-constrained integer programming (IFTCIP) method is developed for supporting
environmental management under uncertainty. The IFTCIP improves upon the existing interval, fuzzy, and two-stage programming
approaches by allowing uncertainties expressed as probability distributions, fuzzy sets, and discrete intervals to be directly
incorporated within a general mixed integer linear programming framework. It has advantages in uncertainty reflection, policy
investigation, risk assessment, and capacity-expansion analysis in comparison to the other optimization methods. Moreover,
it can help examine the risk of violating system constraints and the associated consequences. The developed method is applied
to the planning for facility expansion and waste-flow allocation within a municipal solid waste management system. Violations
of capacity constraints are allowed under a range of significance levels, which reflects tradeoffs between the system cost
and the constraint-violation risk. The results indicate that reasonable solutions for both binary and continuous variables
have been generated under different risk levels. They are useful for generating desired decision alternatives with minimized
system cost and constraint-violation risk under various environmental, economic, and system-reliability conditions. Generally,
willingness to take a higher risk of constraint violation will guarantee a lower system cost; a strong desire to acquire a
lower risk will run into a higher system cost. 相似文献
749.
Mixed-integer linear programs are proposed for siting development and conservation areas in watersheds, addressing economic
objectives (development perimeter and proximity) and ecological objectives. Links between watershed hydrology and ecology
need not be well defined. Parameters for the linear programs are obtained from linearization of the SWAT hydrologic model. 相似文献
750.
Sezen Saçan Aysun Uğur Uğur Sunlu Baha Büyükışık Mehmet Aksu F. Sanem Sunlu 《Environmental monitoring and assessment》2010,161(1-4):575-582
Bottom sediments reflect in general the relative contamination of a sea area. Therefore, a great deal of monitoring work has been dedicated to the analysis of bottom sediments. Izmir Bay is a very important pollution centre in Turkish Aegean coast region due to a densely populated community, industrial complex and maritime transportation, and there are many streams flowing into the bay that pass through a number of industrial and agricultural areas. It had received the majority of domestic and industrial wastewaters until the wastewater treatment plant was constructed. It is well known that sediments play an important role as reservoirs of a fraction of the pollution in aquatic systems. Therefore, sediment samples were collected monthly from three stations which are located in the inner part of the bay during the period January to December 2003. Temporal variations and seasonal changes on their 210Po and 210Pb contents were examined, and the activity concentrations of 210Po and 210Pb were found to vary from 43±6 to 132±12 and 27±5 to 91±9 Bq kg???1 dry wt, respectively. The highest values of those natural radionuclides were measured at Kar??yaka Station because of the current systems of the bay. Seasonally, the 210Pb levels were found to increase during the winter time for all the stations. 相似文献