Orientation of juvenile barn swallows (<Emphasis Type="Italic">Hirundo rustica</Emphasis>) tested in Emlen funnels during autumn migration

Although hirundines have been used extensively in homing experiments, to date no investigation of their migratory orientation has been carried out, despite the well-known migratory habits of many species of this family. This paper reports on a study of the orientation of the barn swallow (Hirundo rustica), a typical diurnal trans-Saharan migrant. Modified Emlen funnels were used to verify the suitability of this species for cage experiments and investigate the role of visual and magnetic cues during the birds first migratory journey. Juvenile swallows were mist-netted at a roost site in central Italy and then tested in a site 19 km apart. Orientation experiments were performed under four experimental conditions: natural clear sky and simulated overcast, in both local and shifted magnetic fields (magnetic North=geographical West). Under clear sky, the swallows tended to orient phototactically toward the best-lit part of the funnel and failed to respond to the magnetic field shift. Under overcast conditions, they oriented northward and modified their directional choices as expected in response to the shifted magnetic North. On the whole, our data indicate that swallows can use magnetic information for compass orientation. Possible explanations for the northward orientation of birds tested under overcast conditions are discussed.Communicated by W. Wiltschko     

Disinfection efficacy on cyst viability of<Emphasis Type="Italic"> Artemia franciscana</Emphasis> (Crustacea),<Emphasis Type="Italic"> Hexarthra fennica</Emphasis> (Rotifera) and<Emphasis Type="Italic"> Fabrea salina</Emphasis> (Ciliophora)

Resting stages (cysts) of Artemia franciscana, Hexarthra fennica and Fabrea salina were exposed for two periods (acute and chronic exposures) to different concentrations of five aquaculture disinfectants (formalin, sodium hypochlorite, potassium permanganate, organic iodine, copper sulphate). The effects of chemical treatments were ranked according to their action on cyst hatching: total inhibition (no cyst hatching), heavy inhibition (significant decrease of cyst hatching), alteration of hatching synchrony, and occurrence of anomalous hatching (death of emerging individuals or birth of malformed organisms). Resting-stage viability was not completely impaired by disinfectant exposures at the ordinary doses used in aquaculture, so that usual treatments are possibly ineffective against the protist and metazoan cysts potentially present in breeding systems. On the other hand, the high resistance shown by cysts suggests the possibility of using these chemicals in live-feed disinfection.     

Orientation of sandhoppers at different points along a dynamic shoreline in southern Tuscany

Orientation experiments were carried out on Talitrus saltator (Crustacea Amphipoda) at four points along 3 km on a dynamic sandy beach inside the Maremma Regional Park (Grosseto, Italy) to highlight behavioural variation related to distance from a river mouth, to erosion or accretion of shoreline, and to human trampling on the beach. Tests were performed using circular transparent Plexiglas arenas, contemporaneously at the four points. Replicates were made in 2 different months (September 2002 and May 2003), on 2–3 successive days, in the morning and afternoon. The distributions of the angles of orientation were compared for the different points and seasons, and multiple regression analysis was performed to test the effects of environmental and intrinsic variables on orientation. Sandhoppers showed the highest scatter at the eroded shoreline, intermediate scatter at the accreting beach most distant from the river mouth, and consistent orientation seaward at the least disturbed point. Orientation of sandhoppers was significantly affected by season, global radiation, time of day, distance from the river mouth, and human trampling. Sex and air humidity were of minor significance in the multiple regression model. The results, on the one hand, confirm plasticity in orientation of sandhoppers living on a dynamic shoreline, and on the other hand, show that variation in orientation could potentially be used as a bioindicator of shoreline changes.Communicated by R. Cattanevo-Vietti, Genova     

Quantifying effects of pollution on biodiversity: a case study of highly diverse molluscan assemblages in the Mediterranean

Structured sampling designs are important in the assessment of environmental impacts of variable ecological systems. Recent developments have provided a useful framework extending existing univariate techniques into a multivariate context. Measures of taxonomic relatedness have also been introduced, which complement existing measures of diversity of assemblages. In this study, the potential effects of sewage discharge on spatial patterns of highly diverse molluscan assemblages in a Mediterranean rocky subtidal habitat were investigated. Nine 20 cm×20 cm quadrats were taken from each of three sites (80 m–100 m apart) within a putatively impacted location near a sewage outfall (I) and at each of two control locations (Cs) by destructive sampling by SCUBA divers at a depth of 3 m–4 m. A total of 5507 specimens of 151 species were collected. The average and the variance in total abundance of molluscs were greater, on average, at I than at Cs. Higher abundances at the sewage outfall were largely driven by greater numbers of juvenile molluscs. The Shannon diversity of molluscs (H′) was significantly lower at I, but no difference among locations was detected for the total number of species (S). In addition, the taxonomic distinctness (Δ*) of molluscs was greater at Cs, although it was more variable at I. Multivariate analyses showed that there was a significant difference in the structure of assemblages at I compared with Cs. The location near the outfall was characterized by greater abundances of several species, including especially the gastropods Pusillina philippi, Bittium latreilli, and Bittium reticulatum. There was also greater variability in the structure of assemblages among sites and among quadrats at control locations compared to those near the outfall. Using a suite of univariate and multivariate measures, including diversity indices, detailed information on taxonomic structure and analyses of variability at different spatial scales, provided useful insights into the effects of sewage impacts on these diverse assemblages. These results also highlighted the importance of analysing measures of variance, as well as mean in considering effects of stress in natural communities.     

High-energy milling to decontaminate soils polluted by polychlorobiphenyls and atrazine

Mechanical energy has been used so far for running chemical reactions and for preparing new materials in absence of solvents. Very recently, the technology has been applied to solve environmental problems. In this paper, we describe the application of high-energy milling (HEM) for the remediation of soils contaminated by chlorinated organic compounds such as polychlorobiphenyls (PCBs) and agrochemicals like atrazine. NaBH₄ and LiAlH₄ have been successfully used for the total dehydrohalogenation of both classes of compounds, leaving a residue lower than 2 ppmw of the starting compound in the treated soil. LiAlH₄ was found to be more active than NaBH₄.     

A reliable indicator of female fecundity: the case of the yellow belly in<Emphasis Type="Italic"> Knipowitschia panizzae</Emphasis> (Teleostei: Gobiidae)

A large body of research has developed on male ornaments and their role in sexual selection while female ornaments have been less investigated. This study focused on the presence, function, and possible role of a sexually dimorphic nuptial trait as an indicator of female quality in a fish species presenting conventional sex roles: the yellow patch on the belly of Knipowitschia panizzae females. Aquarium spawning experiments demonstrated that this ornament is due to dermal pigments, is displayed only when the female is ready to spawn, and is switched off within minutes from the end of egg deposition. This sexual trait is variable in size among females and indicates female fecundity relative to her own body size. As a consequence, female yellow belly appears to be a reliable indicator of female quality as measured by current fecundity. Field data on natural nests highlights that males usually perform parental care on only one egg batch at a time. The modality of egg deposition suggests that males may be limited in their potential reproductive rates by environmental factors. Male limitations in egg care could favour male choosiness and the evolution of female nuptial displays.Communicated by R. Cattaneo-Vietti, Genova     

Phylogenetic relationships within Ovulidae (Gastropoda: Cypraeoidea) based on molecular data from the 16S rRNA gene

Closely interacting groups, such as hosts and parasites, sometimes provide useful models to study evolution and adaptive processes. In Ovulidae, a widespread family of specialised browsing carnivorous molluscs that feed on polyps and tissues of Anthozoa, close association with the host has produced a plethora of adaptations affecting shell morphology, colour pattern and mantle shape. These morphological characters have been widely used in ovulid taxonomy, although putative differences are often based on trivial and inconsistent variations. In this work we used a molecular approach to generate the basal phylogeny of the family. Phylogenetic analyses of mitochondrial 16S rRNA gene sequences of 32 species from 23 genera reveal unexpected taxonomic groupings from the genus up to the family level, as well as unexpected phenomena of homoplasy in conchological features. Since molecular phylogenies also provide an evolutionary framework where character transformations are polarised, we used this basal ovulid phylogeny to explore the specificity of the association and the degree of morphological adaptation to the host.Electronic Supplementary Material Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s00227-005-1566-0.Communicated by R. Cattaneo-Vietti, Genova     

Quantifying inconsistencies in old cadastral maps and their impact on land-use reconstructions

ABSTRACT

Old cadastral maps represent a historical reference dataset for long-term land-use reconstructions. This study presents identification of inconsistencies in the nineteenth century Franziscean cadastre, one of the largest sets of old cadastral maps worldwide, by comparing three versions of the maps and written documents created in the same period. We identified all parcels and their land-use in the four sub-sources in six study areas. The overall share of inconsistencies among 5 771 identified parcels is 7.4%, with the biggest share of inconsistency in agroforestry and forestry classes. The most frequent inconsistencies are of ‘Not differentiable land use’ (n = 212) and ‘Different land-use’ categories across the sub-sources (n = 113). We conclude that the frequency of uncertainties in old cadastral maps may limit the validity of historical land-use reconstructions, affecting the eventual restoration and management efforts based on such data. We provide a summary for the use of Franziscean cadastre.     

Factors inducing the intense burrowing activity of the red-swamp crayfish,<Emphasis Type="Italic"> Procambarus clarkii</Emphasis>, an invasive species

The burrowing activity of the invasive red-swamp crayfish, Procambarus clarkii, was studied along a 25-m-long transect in an irrigation ditch system in Italy. Our objective was to understand the factors inducing this species intense digging, which can result in bank collapse and consequently in severe damage to both agricultural fields and natural ecosystems. Burrow morphology and position, together with their occupancy by crayfish and digging, were recorded once every 6 h for 10 consecutive days. The majority of burrows were simple, although a few had a chimney and were constructed at a farther distance from the water surface than simple burrows. Burrow occupancy and digging, together with their plugged/unplugged status, were constant throughout a 24-h cycle and were not related to any abiotic parameter of the habitat. Crayfish occupied and dug a burrow for a relatively short time (6 h on average). Once abandoned, old burrows were rarely reoccupied and often collapsed, while crayfish excavated new ones. As a result, the overall number of burrows increased. This massive use of banks by P. clarkii seems to be related to soil composition and humidity, which favour crayfish digging but also cause the easy collapse of burrows.     

Removal and pre-concentration of phenolic species onto beta-cyclodextrin modified poly(hydroxyethylmethacrylate-ethyleneglycoldimethacrylate) microbeads

Poly(Hydroxyethylmethacrylate-Ethyleneglycoldimethacrylate), poly(HEMA-EGDMA), microbeads with 150-200 microm in size, was prepared by suspension polymerization. Beta-cyclodextrin was modified onto the polymeric microbeads using glutaraldahyde activation in an acidic medium at pH=2.5. FT-IR and TGA were used for the characterization of modified polymers and the determination of the nature of the interaction between phenolic compounds and the modified polymeric microbeads. Plain and beta-cyclodextrin modified microbeads were used in adsorption-desorption studies of phenolic species in single solution. Adsorption capacities of the phenolic species onto the plain microbeads were found to be 28.2, 17.0, 14.3, 9.8, and 1.92 mg/g polymer for o-chloro phenol, p-nitro phenol, p-chloro phenol, o-nitro phenol, and phenol, respectively. However, for beta-cyclodextrin modified microbeads, adsorption capacity of phenolic species was determined as 274, 365, 128, 182, and 87 mg/g for phenol, o-nitro phenol, p-nitro phenol, o-chloro phenol, and p-chloro phenol, respectively. Desorption ratio for the phenolic species was more than 90%, except for o-nitro phenol. Detection limits of the phenolic species were improved at least 500-fold for UV-Vis spectrophotometric detection, after the pre-concentration of all phenolic species used in this study. Adsorption time for the phenolic species onto beta-cyclodextrin-modified poly(HEMA-EGDMA) microbeads was found to be reasonable short (10-60 min) and suitable for the applications. Also, synthesized microbeads were useful for the repeated use for the removal and pre-concentration of phenolic species.