Tolerating an infection: an indirect benefit of co-founding queen associations in the ant Lasius niger

Pathogens exert a strong selection pressure on organisms to evolve effective immune defences. In addition to individual immunity, social organisms can act cooperatively to produce collective defences. In many ant species, queens have the option to found a colony alone or in groups with other, often unrelated, conspecifics. These associations are transient, usually lasting only as long as each queen benefits from the presence of others. In fact, once the first workers emerge, queens fight to the death for dominance. One potential advantage of co-founding may be that queens benefit from collective disease defences, such as mutual grooming, that act against common soil pathogens. We test this hypothesis by exposing single and co-founding queens to a fungal parasite, in order to assess whether queens in co-founding associations have improved survival. Surprisingly, co-foundresses exposed to the entomopathogenic fungus Metarhizium did not engage in cooperative disease defences, and consequently, we find no direct benefit of multiple queens on survival. However, an indirect benefit was observed, with parasite-exposed queens producing more brood when they co-founded, than when they were alone. We suggest this is due to a trade-off between reproduction and immunity. Additionally, we report an extraordinary ability of the queens to tolerate an infection for long periods after parasite exposure. Our study suggests that there are no social immunity benefits for co-founding ant queens, but that in parasite-rich environments, the presence of additional queens may nevertheless improve the chances of colony founding success.     

Bechstein’s bats maintain individual social links despite a complete reorganisation of their colony structure

Several social mammals, including elephants and some primates, whales and bats, live in multilevel societies that form temporary subgroups. Despite these fission–fusion dynamics, group members often maintain long-term bonds. However, it is unclear whether such individual links and the resulting stable social subunits continue to exist after a complete reorganisation of a society, e.g. following a population crash. Here, we employed a weighted network analysis on 7,109 individual roosting records collected over 4 years in a wild Bechstein’s bat colony. We show that, in response to a strong population decline, the colony’s two stable social subunits fused into a non-modular social network. Nevertheless, in the first year after the crash, long-term bonds were still detectable, suggesting that the bats remembered previous individual relationships. Our findings are important for understanding the flexibility of animal societies in the face of dramatic changes and for the conservation of social mammals with declining populations.

     

Life cycle comparison of hydrothermal liquefaction and lipid extraction pathways to renewable diesel from algae

Algae biomass is an attractive biofuel feedstock when grown with high productivity on marginal land. Hydrothermal liquefaction (HTL) produces more oil from algae than lipid extraction (LE) does because protein and carbohydrates are converted, in part, to oil. Since nitrogen in the algae biomass is incorporated into the HTL oil, and since lipid extracted algae for generating heat and electricity are not co-produced by HTL, there are questions regarding implications for emissions and energy use. We studied the HTL and LE pathways for renewable diesel (RD) production by modeling all essential operations from nutrient manufacturing through fuel use. Our objective was to identify the key relationships affecting HTL energy consumption and emissions. LE, with identical upstream growth model and consistent hydroprocessing model, served as reference. HTL used 1.8 fold less algae than did LE but required 5.2 times more ammonia when nitrogen incorporated in the HTL oil was treated as lost. HTL RD had life cycle emissions of 31,000 gCO₂ equivalent (gCO₂e) compared to 21,500 gCO₂e for LE based RD per million BTU of RD produced. Greenhouse gas (GHG) emissions increased when yields exceeded 0.4 g HTL oil/g algae because insufficient carbon was left for biogas generation. Key variables in the analysis were the HTL oil yield, the hydrogen demand during upgrading, and the nitrogen content of the HTL oil. Future work requires better data for upgrading renewable oils to RD and requires consideration of nitrogen recycling during upgrading.     

Warm forming die design,part I: Experimental validation of a novel thermal finite element modeling code

Finite element analysis (FEA) has become an invaluable tool in the design of sheet metal stamping dies and processes. FEA has gained widespread acceptance as the best method of optimizing dies for conventional stamping processes. More recently, FEA has been shown to be an effective method of designing tooling for sheet forming processes. In this work, an FEA based approach is applied to the warm stamping (warm forming) process. This work introduces a new thermal finite element analysis software called PASSAGE®/Forming (PASSAGE) that enables the up-front design of the thermal management of warm forming dies. This thermal finite element analysis software is designed to specifically handle the forming and optimization scenarios related to the heating of a stamping die while minimizing user interface time. In this work, PASSAGE has been applied to a simple block of steel embedded with cartridge heaters to validate the prediction capability of this software under two different heating conditions. The results show that PASSAGE is capable of predicting the actual steady-state temperature distribution within the block with an acceptable level of accuracy while yielding notable information to the user with respect to specifying power requirements. A finite element software package like PASSAGE is a valuable tool that will aid greatly in the implementation of warm forming as a manufacturing process beyond the scope of the laboratory and into production.     

Part based process performance monitoring (PbPPM)

The performances of completed manufacturing processes were evaluated using the surface response to excitation (SuRE) and Lamb wave methods. Both methods used the same piezoelectric elements attached to the surface of the workpiece. The SuRE method and the Lamb wave method were used to identify the structural changes created by welding, drilling, coating, filling a slot with glue, and composite patching. This study indicates that the tested methods are feasible for part based process performance monitoring (PbPPM) which evaluates the quality of the completed process with the sensors attached to the workpiece.     

Presence of Torque Teno Virus (TTV) in Tap Water in Public Schools from Southern Brazil

Torque teno virus (TTV) was surveyed in tap water collected in schools from three municipalities located in the south of Brazil. TTV genomes were found in 11.7 % (4/34) of the samples. TTV DNA was detected in 10.5 % (2/19) of the samples collected at the city of Caxias do Sul and in 25 % (2/8) of the samples from Pelotas. Those cities have a low rate of sewage treatment. All samples from Santa Cruz do Sul, which has nearly 92 % of its sewage treated, were negative. These results suggest that the amount of sewage treated may have an effect on the detection rates of TTV DNA in drinking water in a given urban area, showing a mild negative correlation (r = ?0.76), when comparing the percentage of sewage treatment to the detection of TTV genomes. The detection rate of TTV was also compared with Escherichia coli, showing a strong correlation (r = 0.97), indicating that TTV may be a suitable marker of fecal contamination.     

Coastal flooding,climate change and environmental justice: identifying obstacles and incentives for adaptation in two metropolitan Boston Massachusetts communities

We explored the possible future impacts of increased coastal flooding due to sea level rise and the potential adaptation responses of two urban, environmental justice communities in the metropolitan Boston area of Massachusetts. East Boston is predominantly a residential area with some industrial and commercial activities, particularly along the coastal fringe. Everett, a city to the north of Boston, has a diversified industrial and commercial base. While these two communities have similar socioeconomic characteristics, they differ substantially in the extent to which residents would be impacted by increased coastal flooding. In East Boston, a large portion of residents would be flooded, while in Everett, it is the commercial/industrial districts that are primarily vulnerable. Through a series of workshops with residents in each community, we found that the target populations do not have an adaptation perspective or knowledge of any resources that could assist them in this challenge. Furthermore, they do not feel included in the planning processes within their communities. However, a common incentive for both communities was an intense commitment to their communities and an eagerness to learn more and become actively engaged in decisions regarding climate change adaptation. The lessons that can be applied to other studies include 1) images are powerful tools in communicating concepts, 2) understanding existing cultural knowledge and values in adaptation planning is essential to the planning process and 3) engaging local residents at the beginning of the process can create important educational opportunities and develop trust and consensus that is necessary for moving from concept to implementation.     

Dimensional analysis and scaling in mechanical mixing for fabrication of metal matrix nanocomposites

For a successful enhancement of mechanical properties of metal matrix nanocomposites, a homogeneous nanoparticle dispersion and distribution in the solidified metal is required. Mechanical mixing can be used for initial break-up of agglomerates, and its study can be simplified with dimensional analysis. Using this technique, mixing time and vortex height were assessed while varying fluid properties, impeller angle, and angular speed. Three relevant dimensionless numbers were recognized: the Reynolds (Re), Froude and Galilei (Ga) numbers. Based on blade and impeller shaft angles, a modified Froude number (Fr*) was defined. These parameters were calculated experimentally, varying angular speed from 200 to 1000 rpm for three different impeller angles: 0°, 15° and 30°. This procedure was performed with three fluids: water, and two aqueous glycerin solutions (25% and 50% by volume). Digital images were taken and processed to measure vortex height. Mixing time was measured for water at 0° impeller angle, angular speed ranging from 200 to 1200 rpm. Results showed an optimal dimensionless mixing time with respect to Re. A linear relationship was found between dimensionless vortex height and Fr*. The first had a second order polynomial relationship with the product ReFr*, regardless of impeller angle. This relationship, together with the Ga, specific for each fluid, allows scaling the results to other fluids such as molten pure aluminum. This study allows experimenting in simpler systems that involve transparent fluids, room temperature and low cost, to then elaborate a prediction of vortex height in fluids where measurements are difficult and costly, such as molten metals.     

Building regional priorities in forests for development and adaptation to climate change in the Congo Basin

Indentifying common priorities in shared natural resource systems constitutes an important platform for implementing adaptation and a major step in sharing a common responsibility in addressing climate change. Predominated by discourses on REDD + (Reduced Emissions from Deforestation and Forest Degradation and conservation, sustainable management of forests and enhancement of forest carbon stocks in developing countries) with little emphasis on adaptation there is a risk of lack of policy measures in addressing climate change in the Congo Basin. Forest products and ecosystem services provide security portfolios for the predominantly rural communities, and play major roles in national development programmes in both revenue and employment opportunities. Thus, raising the profile of forests in the policy arena especially in the twin roles of addressing climate change in mitigation and adaptation and achieving resilient development is crucial. Within the framework of the Congo Basin Forests and Climate Change Adaptation project (COFCCA) project, science policy dialogue was conducted to identify and prioritize forest based sectors vulnerable to climate change but important to household livelihoods and national development. The goal of the prioritization process was for the development of intervention in forest as measures for climate change adaptation in Central Africa. Participants constituted a wide range of stakeholders (government, Non Governmental Organizations, research institutions, universities, community leaders, private sectors etc.) as representatives from three countries directly involved in the project: Cameroon, Central African Republic and Democratic Republic of Congo. Building on national priorities, four forest related sectors were identified as common priorities at the regional level for focus on climate change adaptation. These sectors included: (1) energy with emphasis on fuel wood and Charcoal; (2) Water principally quality, quantity, accessibility, etc.; (3) Food with emphasis on Non Timber Forest Products, and (4) Health linked to healthcare products (medicinal plants). Using these prioritized sectors, the project focused on addressing the impacts of climate change on local communities and the development of adaptation strategies in the three pilot countries of the Congo Basin region. The four sectors constitute the key for development in the region and equally considered as priority sectors in the poverty reduction papers. Focused research on these sectors can help to inject the role of forests in national and local development and their potentials contributions to climate change adaptation in national and public discourses. Mainstreaming forest for climate change adaptation into national development planning is the key to improve policy coherence and effectiveness in forest management in the region.