Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites

This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater zones: (1) subsoil, (2) subsoil to bedrock interface, and (3) bedrock. The gene copy concentrations (GCCs), as gene copies per liter, for bacterial 16S rRNA genes and the denitrifying functional genes, nirK, nirS, and nosZ, were determined using quantitative polymerase chain reaction assays. The results were related to gaseous nitrogen emissions and to the physicochemical properties of the four sites. Overall, nirK and nirS abundance appeared to show no significant correlation to N₂O production (P?=?0.9989; P?=?0.3188); and no significant correlation was observed between nosZ and excess N₂ concentrations (P?=?0.0793). In the majority of piezometers investigated, the variation of nirK and nirS gene copy concentrations was considered significant (P?<?0.0001). Dissolved organic carbon (DOC) decreased with aquifer depth and ranged from 1.0–4.0 mg l^?1, 0.9–2.4 mg l^?1, and 0.8–2.4 mg l^?1 within piezometers located in the subsoil, subsoil/bedrock interface, and bedrock depths, respectively. The availability of increasing DOC and the depth of the water table were positively correlated with increasing nir and nosZ GCCs (P?=?0.0012). A significant temporal correlation was noted between nirS and piezometer depth (P?<?0.001). Interestingly, the nirK, nirS, and nosZ GCCs varied between piezometer depths within specific sites, while GCCs remained relatively constant from site to site, thus indicating no direct impact of agricultural land management strategies investigated on denitrifier abundance.     

Chemical amendment of pig slurry: control of runoff related risks due to episodic rainfall events up to 48 h after application

Losses of phosphorus (P) from soil and slurry during episodic rainfall events can contribute to eutrophication of surface water. However, chemical amendments have the potential to decrease P and suspended solids (SS) losses from land application of slurry. Current legislation attempts to avoid losses to a water body by prohibiting slurry spreading when heavy rainfall is forecast within 48 h. Therefore, in some climatic regions, slurry spreading opportunities may be limited. The current study examined the impact of three time intervals (TIs; 12, 24 and 48 h) between pig slurry application and simulated rainfall with an intensity of 11.0?±?0.59 mm h^?1. Intact grassed soil samples, 1 m long, 0.225 m wide and 0.05 m deep, were placed in runoff boxes and pig slurry or amended pig slurry was applied to the soil surface. The amendments examined were: (1) commercial-grade liquid alum (8 % Al₂O₃) applied at a rate of 0.88:1 [Al/ total phosphorus (TP)], (2) commercial-grade liquid ferric chloride (38 % FeCl₃) applied at a rate of 0.89:1 [Fe/TP] and (3) commercial-grade liquid poly-aluminium chloride (10 % Al₂O₃) applied at a rate of 0.72:1 [Al/TP]. Results showed that an increased TI between slurry application and rainfall led to decreased P and SS losses in runoff, confirming that the prohibition of land-spreading slurry if heavy rain is forecast in the next 48 h is justified. Averaged over the three TIs, the addition of amendment reduced all types of P losses to concentrations significantly different (p?<?0.05) to those from unamended slurry, with no significant difference between treatments. Losses from amended slurry with a TI of 12 h were less than from unamended slurry with a TI of 48 h, indicating that chemical amendment of slurry may be more effective at ameliorating P loss in runoff than current TI-based legislation. Due to the high cost of amendments, their incorporation into existing management practices can only be justified on a targeted basis where inherent soil characteristics deem their usage suitable to receive amended slurry.     

BRC4Env,a network of Biological Resource Centres for research in environmental and agricultural sciences

The Biological Resource Centre for the Environment BRC4Env is a network of Biological Resource Centres (BRCs) and collections whose leading objectives are to improve the visibility of genetic and biological resources maintained by its BRCs and collections and to facilitate their use by a large research community, from agriculture research to life sciences and environmental sciences. Its added value relies on sharing skills, harmonizing practices, triggering projects in comparative biology, and ultimately proposing a single-entry portal to facilitate access to documented samples, taking into account the partnership policies of research institutions as well as the legal frame which varies with the biological nature of resources. BRC4Env currently includes three BRCs: the Centre for Soil Genetic Resources of the platform GenoSol, in partnership with the European Conservatory of Soil Samples; the Egg Parasitoids Collection (EP-Coll); and the collection of ichthyological samples, Colisa. BRC4Env is also associated to several biological collections: microbial consortia (entomopathogenic bacteria, freshwater microalgae…), terrestrial arthropods, nematodes (plant parasitic, entomopathogenic, animal parasitic...), and small mammals. The BRCs and collections of BRC4Env are involved in partnership with academic scientists, as well as private companies, in the fields of medicinal mining, biocontrol, sustainable agriculture, and additional sectors. Moreover, the staff of the BRCs is involved in many training courses for students from French licence degree to Ph.D, engineers, as well as ongoing training.     

Climate change impacts detection in dry forested ecosystem as indicated by vegetation cover change in —Laikipia,of Kenya

The objective of the study was to detect and identify land cover changes in Laikipia County of Kenya that have occurred during the last three decades. The land use types of study area are six, of which three are the main and the other three are the minor. The main three, forest, shrub or bush land and grassland, changed during the period, of which grasslands reduced by 5864 ha (40%), forest by 3071 ha (24%) and shrub and bush land increased by 8912 ha (43%). The other three minor land use types were bare land which had reduced by 238 ha (45%), river bed vegetation increased by 209 ha (72%) and agriculture increased by 52 ha (600%) over the period decades. Differences in spatiotemporal variations of vegetation could be largely attributed to the effects of climate factors, anthropogenic activities and their interactions. Precipitation and temperature have been demonstrated to be the key climate factors for plant growth and vegetation development where rainfall decreased by 200 mm and temperatures increased by 1.5 °C over the period. Also, the opinion of the community on the change of land use and management was attributed to climate change and also adaptation strategies applied by the community over time. For example unlike the common understanding that forest resources utilisation increases with increasing human population, Mukogodo dry forested ecosystem case is different in that the majority of the respondents (78.9%) reported that the forest resource use was more in that period than now and also a similar majority (74.2%) had the same opinion that forest resource utilisation was low compared to last 30 years. In Yaaku community, change impacts were evidenced and thus mitigation measures suggested to address the impacts which included the following: controlled bush management and indigenous grass reseeding programme were advocated to restore original grasslands, and agricultural (crop farming) activities are carried out in designated areas outside the forest conservation areas (ecosystem zoning) all in consultation with government (political class), community and other stakeholders. Groups are organised (environmental management committee) to address conservation, political and vulnerability issues in the pastoral dry forested ecosystem which will sustain pastoralism in the ecosystem.     

Morphological Diversity and Variability of Sympatric Populations of Crucian and Prussian Carps in Radionuclide Contaminated Lakes in the Southern Urals

The variation in the body shape of crucian and prussian carps has been studied by methods of geometric morphometrics in sympatric populations from two geographically close lakes in the Southern Urals subjected to different degrees of contamination with anthropogenic radionuclides. Along with particular interspecies features, interpopulation differences have been detected as multidirectional reorganization of cyprinid morphogenesis under different ecological conditions of adjacent lakes. The morphogenetic divergence of crucian carp from adjacent lakes is almost two times smaller than that of the prussian carp coexisting with them. The increased intragroup morphodiversity in prussian carp indicates incomplete adaptation of the fish to anthropogenic radionuclide contamination of the lakes and their high morphogenetic plasticity and high adaptive potential than in crucian carp.     

Morphogenetic Effects of Drought and Nonselective Elimination in Population of Bank Vole (<Emphasis Type="Italic">Clethrionomys glareolus</Emphasis>) in Southern Taiga Subzone

Methods of geometric morphometrics have been used to estimate the influence ratio of nonselective elimination and drought factors on variation in the shape and size of the mandible in the population of bank vole (Clethrionomys glareolus Schreb.) in the southern taiga subzone. Nonselective elimination of rodent populations for medical and sanitary purposes was carried out in a felling site located in a focus of hemorrhagic fever, in the spring of a climatically normal year and of a dry year. The summer samples of mature young of the year from control bank vole colonies and impact colonies (i.e., recovered after deratization) in adjacent years have been compared. The results show that drought, nonselective elimination, and the interaction of these factors have significant effects on the size and shape of the mandible. Changes in its shape under drought conditions are largely due to allometry. Morphogenetic effects of nonselective elimination are highly repeatable between climatically different years. A significantly higher level of within-group morphological disparity (MNND) of the undisturbed control cenopopulation in a dry year has been revealed, which indirectly indicates a stronger destabilization of morphogenesis upon exposure to the autecological factor. Every ecological factor contributes to the development of specific configurations of the mandible; i.e., it induces certain changes in morphogenesis in response to aut- and synecological effects and their combination.     

Climate change impacts on critical international transportation assets of Caribbean Small Island Developing States (SIDS): the case of Jamaica and Saint Lucia

This contribution presents an assessment of the potential vulnerabilities to climate variability and change (CV & C) of the critical transportation infrastructure of Caribbean Small Island Developing States (SIDS). It focuses on potential operational disruptions and coastal inundation forced by CV & C on four coastal international airports and four seaports in Jamaica and Saint Lucia which are critical facilitators of international connectivity and socioeconomic development. Impact assessments have been carried out under climatic conditions forced by a 1.5 °C specific warming level (SWL) above pre-industrial levels, as well as for different emission scenarios and time periods in the twenty-first century. Disruptions and increasing costs due to, e.g., more frequent exceedance of high temperature thresholds that could impede transport operations are predicted, even under the 1.5 °C SWL, advocated by the Alliance of Small Island States (AOSIS) and reflected as an aspirational goal in the Paris Climate Agreement. Dynamic modeling of the coastal inundation under different return periods of projected extreme sea levels (ESLs) indicates that the examined airports and seaports will face increasing coastal inundation during the century. Inundation is projected for the airport runways of some of the examined international airports and most of the seaports, even from the 100-year extreme sea level under 1.5 °C SWL. In the absence of effective technical adaptation measures, both operational disruptions and coastal inundation are projected to increasingly affect all examined assets over the course of the century.     

Effects of geomorphology, habitat, and spatial location on fish assemblages in a watershed in Ohio, USA

In this paper, we evaluate relationships between in-stream habitat, water chemistry, spatial distribution within a predominantly agricultural Midwestern watershed and geomorphic features and fish assemblage attributes and abundances. Our specific objectives were to: (1) identify and quantify key environmental variables at reach and system wide (watershed) scales; and (2) evaluate the relative influence of those environmental factors in structuring and explaining fish assemblage attributes at reach scales to help prioritize stream monitoring efforts and better incorporate all factors that influence aquatic biology in watershed management programs. The original combined data set consisted of 31 variables measured at 32 sites, which was reduced to 9 variables through correlation and linear regression analysis: stream order, percent wooded riparian zone, drainage area, in-stream cover quality, substrate quality, gradient, cross-sectional area, width of the flood prone area, and average substrate size. Canonical correspondence analysis (CCA) and variance partitioning were used to relate environmental variables to fish species abundance and assemblage attributes. Fish assemblages and abundances were explained best by stream size, gradient, substrate size and quality, and percent wooded riparian zone. Further data are needed to investigate why water chemistry variables had insignificant relationships with IBI scores. Results suggest that more quantifiable variables and consideration of spatial location of a stream reach within a watershed system should be standard data incorporated into stream monitoring programs to identify impairments that, while biologically limiting, are not fully captured or elucidated using current bioassessment methods.     

A Stream–Wetland–Riparian (SWR) index for assessing condition of aquatic ecosystems in small watersheds along the Atlantic slope of the eastern U.S.

As part of a regional study by the Atlantic Slope Consortium (ASC) to develop ecological and socioeconomic indicators of aquatic ecosystem condition, we developed and tested a protocol for rapidly assessing condition of the stream, wetland, and riparian components of freshwater aquatic ecosystems. Aspects of hydrology, vegetation, in-stream and wetland characteristics, and on-site stressors were measured in the field. The resulting metrics were used to develop an index of overall condition, termed the Stream–Wetland–Riparian (SWR) Index. Values of this Index were compared to existing biotic indices and chemical measures, and to a Landscape Index created using satellite-based land cover data and a geographic information system (GIS). Comparisons were made at several levels of spatial aggregation and resolution, from site to small watershed. The SWR Index and associated Landscape Indices were shown to correlate highly with biological indicators of stream condition at the site level and for small contributing areas. The landscape patterns prevalent throughout the entire watershed do not necessarily match the patterns found adjacent to the stream network. We suggest a top-down approach that managers can use to sequentially apply these methods, to first prioritize watersheds based on a relative condition measure provided by the Landscape Index, and then assess condition and diagnose stressors of aquatic resources at the subwatershed and site level.