Imposex and butyltin concentrations in Bolinus brandaris (Gastropoda: Muricidae) from the northern Tunisian coast

The imposex incidence and butyltin concentration i.e. tributyltin with its di- and mono-substituted metabolites were investigated in the muricid Bolinus brandaris sampled from two sites on the northern Tunisian coast (the Lagoon of Bizerta and the small Gulf of Tunis). Both populations had imposex, with stages of imposex development varying between VDS 1 and VDS 4.3. All imposex indices (imposex frequency (I %), female penis length, female vas deferens length, vas deferens sequence index, relative penis length index, and vas deferens length index) were significantly higher in snails from the Bizerta lagoon. Butyltins were detected in the whole tissues of both sexes from the two sites. TBT levels were higher in gastropods collected from the lagoon of Bizerta (12.65 ±1.48 ng Sn g^???1 dw in female and 15.21 ±1.13 ng Sn g^???1 dw in male) than in individuals from the Gulf of Tunis (10.71 ±1.26 ng Sn g^???1 dw in female and 11.65 ±1.63 ng Sn g^???1 dw in male), corroborating the data of imposex analysis. These results confirmed that B. brandaris could be used as a bioindicator species of butyltin pollution in the studied areas. In addition, this study provided baseline data that could serve for long-term monitoring of TBT pollution in Tunisia, since legislation to reduce the use of TBT-based antifouling paints has not been introduced yet.     

Shell disturbances and butyltins burden in commercial bivalves collected from the Bizerta lagoon (northern Tunisia)

Shell disturbances and soft tissues butyltin burden were investigated in commercial bivalves Lithophaga lithophaga, Mytilus galloprovincialis, Solen marginatus and Crassostrea gigas from the Bizerta lagoon. Shell disturbances were found in all bivalves, being scarce in S. marginatus. In the internal valve of L. lithophaga, burrowing annelids and sipunculids living inside galleries were observed, while in the external valve, brown-blackish or white stains were found. In M. galloprovincialis, a yellowish mass located at the shell anterior side was found fixed firmly to the pearly layer by a hard brownish structure covering some annelid elliptic eggs. In the internal shell layer of some specimens collected in April, embryos belonging to tubiculous annelids at various developmental stages were observed. In C. gigas, shell thickening was revealed in some specimens corresponding to white doughy deposits at the internal valve and between shell layers. In S. marginatus, only one specimen showing a cavity at the posterior site was found. Total butyltin concentrations in the studied bivalves varied between 30 and 245?ng/g dry weight with tributyltin (TBT) being the predominant compound. The highest concentration was recorded in L. lithophaga collected from the Bizerta Bay and the lowest concentration in S. marginatus from Maghraoua. This study provided baseline data that could serve for long-term monitoring of TBT pollution in Tunisia, since legislation to reduce the use of TBT-based antifouling paints has not been introduced yet.     

Quality assessment of composts prepared with olive mill wastewater and agricultural wastes

The co-composting of solid residue from olive oil production process, exhausted olive cake (EOC), with poultry manure (PM) watered with olive mill wastewater (OMW) was considered as an efficient method for the treatment of olive oil extraction effluent having high organic content including phenolic polluting compounds. The process was carried out by using three aerated windrows of variable compositions. OMW was used continuously during the bio-oxidative period, which lasted three months, to replace water for windrow moistening. The main process parameters (temperature, pH, humidity and C/N) were monitored over four months to ascertain the maturity of the compost. The composting process lasted four months during which 26 moistenings of the mixtures were performed with OMW or water to keep moisture within the ideal range of 45-60% (w/w). At the maturity stage, the C/N ratios were less than 16, pH of the resulting products were slightly alkaline (pH=8) and electrical conductivity was relatively high in the OMW mixtures (5.46-5.48 Sm(-1)) when compared with water application. Nitrates increased (0.16-0.42%) and phenol contents were reduced by more than 49%. Mature composts were then used as an amendment for potato production in a field where no inhibitory effect was observed. Potato productivity increased 10-23% as a result of compost application. No noticeable negative impact of OMW on the soil system was observed. Phenolic compound concentrations in the stabilised composts were comparable in the three studied mixtures (different sites) and averaged 0.24%. Considering previous results and this three year study, it has been observed that the benefit of these composts demonstrated the potential sustainable agronomic production of potato while using locally available recycled organic materials.     

Case study analysis of the financial impact of catastrophic safety events

Several catastrophic events in the process industry have caused extensive damage to life and property and have forever changed the process safety landscape. Despite the commitment from the industry, incidents still occur with similar root causes, impacting major companies in various ways. This work examines the financial impact of such incidents on the stability and future of affected companies in different industries. Several devastating incidents were analyzed to evaluate the direct impact in the short- and long-term and to discuss factors influencing the ability of affected companies to withstand the consequence of catastrophic events.     

Effects of environmentally relevant levels of polyethylene microplastic on Mytilus galloprovincialis (Mollusca: Bivalvia): filtration rate and oxidative stress

Environmental Science and Pollution Research - The objective of the present study was to evaluate the potential toxic effects of polyethylene microplastics (PE-MPs) (40–48 μm) on the...     

Acute toxicity and teratogenicity of carbaryl (carbamates), tebufenpyrad (pyrazoles), cypermethrin and permethrin (pyrethroids) on the European sea bass (Dicentrarchus labrax L, 1758) early life stages

Environmental Science and Pollution Research - The toxicity of carbaryl, tebufenpyrad, cypermethrin and permethrin was evaluated in European sea bass Dicentrarchus labrax during the embryonic and...     

Potential of Nassarius nitidus for monitoring organotin pollution in the lagoon of Bizerta （northern Tunisia）

Imposex and butyltin burden were assessed in Nassarius nitidus, Bolinus brandaris and Hexaplex trunculus collected at five stations in the Bizerta lagoon. Biological analysis showed that imposex followed type (a) in N nitidus (distal evolution), against type (d) in the two muricids (proximal evolution). Imposex indices were higher in sites located nearby sources of tributyltin and N nitidus was the least affected species of the five sites, followed by B. brandaris and H. trunculus. Butyltin analysis showed lower accumulation in N nitidus followed by H. trunculus and B. brandaris. This study has allowed the gathering of data on imposex in a snail studied for the first time in Tunisia (N. nitidus). It suggests the possibility of using such snail as a complementary species for organotin monitoring programs in the Mediterranean and further confirmed that H. trunculus is the most suitable species for such investigations.     

Determination of chlorinated pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers in human milk from Bizerte (Tunisia) in 2010

Thirty persistent organohalogen compounds including organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) were determined in breast milk samples (n = 36) of primipara and multipara mothers from Bizerte in 2010. The analytical procedure involved the application of liquid-liquid extraction and gas chromatography with electron capture detector (GC-ECD) or mass spectrometry detector (GC-MS) for identification and quantification. Organohalogen compounds were found in all the analyzed samples, with predominance of p,p′-DDE, p,p′-DDT, p,p′-DDD, HCB and PCBs. The mean concentration of ∑DDTs in breast milk was 1163.9 ng g⁻¹ lipid wt. The ratio of p,p′-DDE/p,p′-DDT was low, suggesting that there is fresh intake of commercial DDT products in Bizerte. The mean levels of HCB and PCB were 286.8 and 331.2 ng g⁻¹ lipid wt respectively. These results were compared with the levels obtained in a previous study carried out in the same area in 2003. A general decrease of ∑DDTs levels and an increase of PCB levels were observed. Among the 10 PBDE congeners evaluated, BDE-28, BDE-47, BDE-99, BDE-66, BDE-138, BDE-100, BDE-154, BDE-153, and BDE-183 were detected in the analyzed samples at different frequency. The total PBDE concentrations ranged from 2.5 to 22.6 ng g⁻¹ lipid wt in the samples, with a mean and median value of 10.7 and 9.8 ng g⁻¹ lipid wt respectively. To our knowledge, this is the first data of PBDEs in Tunisian human milk. The present study shows that age and parity are factors influencing the levels of some organohalogen compounds in human milk.