Mycorrhizal induction of phenolic compounds and antioxidant properties of fungi and seedlings during the early steps of symbiosis

Ectomycorrhizal (ECM) symbiosis plays a major role in nutrient cycling and the functioning of forest ecosystems. Trees with well-developed ectomycorrhizas are more resistant to environmental stresses such as drought and to biotic stresses such as root pathogens. The establishment of ECM symbiosis is triggered by signals produced by both partners. These signals lead to morphological changes and a complex development of specific structures in both the plant root and the fungus. The development of the ECM fungi, Paxillus involutus and Pisolithus arhizus, in the presence and the absence of the symbiont—Pinus pinaster—was evaluated as well as their antioxidant properties and phenolic compounds composition in response to the symbiotic association. ECM fungi grew less in the presence of P. pinaster, with P. arhizus being less affected in growth and thus being more adapted to this association. Protocatechuic acid was found only in P. involutus, while P. pinaster roots, both in association and isolated, proved to have other phenolic acids, such as p-hydroxybenzoic and p-coumaric acids. The symbiosis between P. involutus and P. pinaster had no major effects on the symbionts, while the association between P. arhizus and P. pinaster seems to generally decrease the antioxidant effects of both symbionts, despite the increase in p-coumaric and cinnamic acids in the ECM fungi.     

Toxic and essential elements in blood from delivering women in selected areas of São Paulo State, Brazil

This study was designed to evaluate the degree of environmental contamination and possible exposure of pregnant women to toxic elements in seven selected areas of S?o Paulo State, Brazil. The overall median concentration of Mo in maternal blood was 0.53 μg L?1, highly significant differences found between sites (p < 0.0001). Cd was found to be low overall - 0.09 μg L?1 (0.01-0.58 μg L?1) - with mothers from the Coastal and Rural 1 sites having the highest levels (p < 0.016).Median Hg concentration was 0.60 μg L?1 (0.06 μg L?1-4.35 μg L?1); median Pb level was 16.2 μg L?1 (3.5-57.7 μg L?1) and no differences between sites were observed for both metals. Median Mn level was 16.7 μg L?1 (7.0-39.7 μg L?1), being highest in Urban 2 site (p < 0.016). Concentrations of maternal Co were found to range between 0.06 μg L?1 and 1.1 μg L?1 (median 0.25 μg L?1) and As level was 0.60 μg L?1 (0.10-3.8 μg L?1) overall, with no statistical significance between sites for Co and As. Median Se concentrations were found to be 64 μg L?1 (36-233 μg L?1), with the highest median levels found in Urban 3 site; site differences were statistically significant (p < 0.0001). Correlation for each element (between paired maternal and cord blood) was measured only in Rural site 1; significant correlation was shown for Hg, Pb, Mn and Co (p < 0.05). These findings may be interpreted as indicating low environmental contamination in S?o Paulo State, Brazil. These findings could also indicate that pregnant women have little or no contact with pollutants, possibly due to awareness campaigns carried out by public health practitioners.     

Enhanced reductive dechlorination of PCE in unconsolidated soils

An Interstate Technology and Regulatory Council (ITRC) forum was recently held that focused on six case studies in which bioremediation of dense nonaqueous phase liquids (DNAPLs) was performed. The objective was to demonstrate that there is credible evidence for bioremediation as a viable environmental remediation technology. A discussion of the first case study from the ITRC forum was published in the previous issue of Remediation. This article presents a discussion of the second case study, which involves enhanced reductive dechlorination (ERD) of tetrachloroethene (PCE) in unconsolidated soils—primarily silts and clays with very low permeabilities. The project results indicate that complete reductive dechlorination was achieved and provide encouragement that large amounts of nonaqueous solvent can be brought into the reductive dechlorination treatment process by dissolution and desorption, giving support to the contention that the capacity to attack nonaqueous mass is a prerequisite for any effective treatment of DNAPL source zones. The site geology for this project was relatively unfavorable, and further work is needed to confirm that the ERD technology can economically reach a natural attenuation endpoint for this type of setting. © 2006 Wiley Periodicals, Inc.     

Using crowd-sourced photos to assess seasonal patterns of visitor use in mountain-protected areas

Managing protected areas effectively requires information about patterns of visitor use, but these data are often limited. We explore how geotagged photos on Flickr, a popular photo-sharing social-media site, can generate hotspot maps and distribution models of temporal and spatial patterns of use in two mountain-protected areas of high conservation value. In Aconcagua Provincial Park (Argentina), two routes to the summit of Aconcagua were used in summer, but most visitors stayed close to the main road, using formal and informal walking trails and the Visitor Centre, while in winter, there was very limited visitation. In Kosciuszko National Park (Australia), alpine walking trails were popular in summer, but in winter, most visitors stayed in the lower altitude ski resorts and ski trails. Results demonstrate the usefulness of social-media data alone as well as a complement for visitor monitoring, providing spatial and temporal information for site-specific and park-level management of visitors and potential impacts in conservation areas.     

Prey selection by resident common bottlenose dolphins (tursiops truncatus) in Sarasota Bay, Florida

Prey selection was investigated in wild, resident common bottlenose dolphins, Tursiops truncatus, during the summer months in Sarasota Bay, Florida, USA. Stomach content analyses of 15 dolphins with extensive sighting histories and well-documented distributions were used to determine prey use. Prey availability was assessed by purse seine surveys. We compared the relative abundances of prey available to estimates of prey use at closely matching spatial and temporal scales. G-tests determined that dolphins in this study significantly selected for prey at the species, family, and soniferous/non-soniferous prey levels (G _adj  = 753.98–1,775.93, df = 1–21, p ≤ 0.01). While comprising only 6.3% of the total available prey, soniferous fishes accounted for 51.9% of the total prey consumed. Manly’s standardized forage ratios and 95% Bonferroni confidence intervals determined significant positive selection for soniferous prey and against non-soniferous prey (β^S = 0.9461 vs. β^NS = 0.0539). Dolphins selected against Gerridae, Clupeidae, and Sparidae (β ≤ 0.0014), as well as against all the species within those families (β ≤ 0.0190). It is likely that passive listening for soniferous prey provides an ecological or energetic advantage to cetaceans utilizing this specific foraging technique.     

Erratum to: Prey selection by resident common bottlenose dolphins (Tursiops truncatus) in Sarasota Bay, Florida

Prey selection was investigated in wild, resident common bottlenose dolphins, Tursiops truncatus, during the summer months in Sarasota Bay, Florida, USA. Stomach content analyses of 15 dolphins with extensive sighting histories and well-documented distributions were used to determine prey use. Prey availability was assessed by purse seine surveys. We compared the relative abundances of prey available to estimates of prey use at closely matching spatial and temporal scales. G-tests determined that dolphins in this study significantly selected for prey at the species, family, and soniferous/non-soniferous prey levels (G _adj  = 753.98–1,775.93, df = 1–21, p ≤ 0.01). While comprising only 6.3% of the total available prey, soniferous fishes accounted for 51.9% of the total prey consumed. Manly’s standardized forage ratios and 95% Bonferroni confidence intervals determined significant positive selection for soniferous prey and against non-soniferous prey (β^S = 0.9461 vs. β^NS = 0.0539). Dolphins selected against Gerridae, Clupeidae, and Sparidae (β ≤ 0.0014), as well as against all the species within those families (β ≤ 0.0190). It is likely that passive listening for soniferous prey provides an ecological or energetic advantage to cetaceans utilizing this specific foraging technique.     

Vacuolate-attached filaments: highly productive Ridgeia piscesae epibionts at the Juan de Fuca hydrothermal vents

Vacuolate sulfur bacteria with high morphological similarity to vacuolate-attached filaments previously described from shallow hydrothermal vents (White Point, CA) were found at deep-sea hydrothermal vents. These filamentous bacteria grow in dense mats that cover surfaces and potentially provide a significant source of organic carbon where they occur. Vacuolate-attached filaments were collected near vents at the Clam Bed site of the Endeavour Segment of the Juan de Fuca Ridge and from the sediment surface at Escanaba Trough on the Gorda Ridge. A phylogenetic analysis comparing their 16S rRNA gene sequences to those collected from the shallow White Point site showed that all vacuolate-attached filament sequences form a monophyletic group within the vacuolate sulfur-oxidizing bacteria clade in the gamma proteobacteria. Abundance of the attached filaments was quantified over the length of the exterior surface of the tubes of Ridgeia piscesae worms collected from the Clam Bed site at Juan de Fuca yielding a per worm average of 0.070 ± 0.018 cm³ (n = 4). In agreement with previous results for White Point filaments, anion measurements by ion chromatography showed no detectable internal nitrate concentrations above ambient seawater (n = 9). For one R. piscesae tube worm “bush” at the Easter Island vent site, potential gross epibiont productivity is estimated to be 15 to 45× the net productivity of the worms.     

Biogeography of Atlantic and Mediterranean ascidians

Ascidian fauna have been intensively studied in the Atlantic Ocean, adjacent subpolar regions and Mediterranean Sea for the last 20 years. Here, we have described current species distribution patterns and identified nested areas of endemism using parsimony analysis of endemicity (PAE). We also identified diversity hotspots, areas in which species occurred exclusively, and gaps in information about distribution patterns. Finally, we compared ascidian distributions among various proposed biogeographic divisions. The comprehensive literature review provided data on the geographic distribution of 627 species of ascidians. In the West Atlantic, there were three peaks in richness: north Caribbean—96 species, São Paulo and Rio de Janeiro—61 species and the subantarctic region—54 species. In the eastern Atlantic, the greatest richness occurred on Spanish and French coasts—91 species and in Senegal—83 species. In the Mediterranean, the greatest richness was in Spain and France—142 species and Italy—127 species. PAE designated 20 areas of endemism nested within eight larger regions that were more or less in agreement with realms or provinces of previous studies: North Atlantic, Caribbean, southeastern Brazil, Magellanic, Subantarctic, Tropical West Africa, South Africa, and Mediterranean. Distribution patterns of the Ascidiacea, in general, followed previously proposed divisions of regions and provinces in the Atlantic and adjacent polar regions, but not subdivisions of these regions.     

Assessing sustainable development in urban areas using cognitive mapping and MCDA

As interest in sustainability-related issues has increased over recent years, so too has urban sustainability risen to the fore, in academic, practitioner, and policymaking circles alike. Urban sustainability requires a balance between environmental concerns, the economy, and social development in urban areas. However, over the years, there has been an exponential increase in urban density, accompanied by increased economic activity and high levels of consumption, which have hindered urban planning and made the sustainable management of urban areas more difficult. It has therefore become increasingly necessary to combine the interests of the various stakeholders involved in – or affected by – urban planning measures, in order to achieve a balance between their needs, those of the environment and future generations, and the need for economic development. Sustainability evaluation models can in this sense be considered a baseline condition for sustainable development. However, most existing evaluation systems present limitations in terms of criteria identification and the calculation of the respective trade-offs. To address these issues, the current study aims to combine cognitive mapping and the Analytic Hierarchy Process (AHP) to prioritize the determinants of sustainable development in urban areas. The advantages and limitations of our proposal are also analyzed.     

Policy on wetland impact mitigation

Important policy issues concerning the mitigation of impacts from construction and development affecting wetlands are under examination by the U.S. Congressional Office of Technology Assessment, the Environment and Public Works Committee of the U.S. Senate, and the National Wetlands Technical Council. The issues divide into two main parts: (1) how the current strategy to simplify federal regulation of wetlands is limiting the success of mitigation; and (2) how to change the present strategy for mitigation under the U.S. Clean Water Act, if at all. Requirements for site-specific analysis of impacts and their mitigation requirements are being replaced by simple, uniform national guidelines on impact mitigation; these guidelines are designed to streamline the regulatory process in which the proposed activities typically generate only minor impacts. This trend ignores how the intrinsic values of the affected wetland modify the actual severity of impact. A change in regulatory strategy may be necessary so that the efforts at impact mitigation are scaled to wetland values as well as the magnitude of adverse effects normally expected with particular activities.