Rhizosphere acidification and cadmium uptake by strawberry clover

Strawberry clover (Trifolium fragiferum L.) is a hardy legume found in indigenous or introduced populations throughout the world. Tolerance to saline and alkaline soils, flooding, and heavy metals make it a good prospect for reclamation projects. The research, described here, was conducted to: (i) characterize the morphological variation in plants from available seed sources, (ii) evaluate cadmium uptake and tolerances over a wide range of morphological variants, and (iii) elucidate the variability in the effects of roots on rhizosphere pH and the relationship to cadmium uptake. Seeds from selected accessions were planted in the greenhouse for comparison of morphological variation. The accessions examined had a mean height of 10.7+/-7 cm. Accessions 254916 and 237925 are tall with high rhizosphere pH values and might be useful in phytoremediation. Strawberry clover accessions were also grown hydroponically to examine differences in cadmium uptake. The ability of strawberry clover roots to change rhizosphere pH and take up cadmium was examined using culture tubes containing nutrient agar, a moderate level of cadmium, and a pH indicator dye. The results provided evidence for a negative correlation between rhizosphere pH and cadmium uptake.     

A provider-based water planning and management model--WaterSim 4.0--for the Phoenix Metropolitan Area

Uncertainty in future water supplies for the Phoenix Metropolitan Area (Phoenix) are exacerbated by the near certainty of increased, future water demands; water demand may increase eightfold or more by 2030 for some communities. We developed a provider-based water management and planning model for Phoenix termed WaterSim 4.0. The model combines a FORTRAN library with Microsoft C# to simulate the spatial and temporal dynamics of current and projected future water supply and demand as influenced by population demographics, climatic uncertainty, and groundwater availability. This paper describes model development and rationale. Water providers receive surface water, groundwater, or both depending on their portfolio. Runoff from two riverine systems supplies surface water to Phoenix while three alluvial layers that underlie the area provide groundwater. Water demand was estimated using two approaches. One approach used residential density, population projections, water duties, and acreage. A second approach used per capita water consumption and separate population growth estimates. Simulated estimates of initial groundwater for each provider were obtained as outputs from the Arizona Department of Water Resources (ADWR) Salt River Valley groundwater flow model (GFM). We compared simulated estimates of water storage with empirical estimates for modeled reservoirs as a test of model performance. In simulations we modified runoff by 80%-110% of the historical estimates, in 5% intervals, to examine provider-specific responses to altered surface water availability for 33 large water providers over a 25-year period (2010-2035). Two metrics were used to differentiate their response: (1) we examined groundwater reliance (GWR; that proportion of a providers' portfolio dependent upon groundwater) from the runoff sensitivity analysis, and (2) we used 100% of the historical runoff simulations to examine the cumulative groundwater withdrawals for each provider. Four groups of water providers were identified, and discussed. Water portfolios most reliant on Colorado River water may be most sensitive to potential reductions in surface water supplies. Groundwater depletions were greatest for communities who were either 100% dependent upon groundwater (urban periphery), or nearly so, coupled with high water demand projections. On-going model development includes linking WaterSim 4.0 to the GFM in order to more precisely model provider-specific estimates of groundwater, and provider-based policy options that will enable "what-if" scenarios to examine policy trade-offs and long-term sustainability of water portfolios.     

Environmental accounting and statistics

The views expressed here are the author's and do not necessarily reflect an expression of opinion on the part of the United Nations. The objective of sustainable development is to integrate environmental concerns with mainstream socio-economic policies. Integrated policies need to be supported by integrated data. Environmental accounting achieves this integration by incorporating environmental costs and benefits into conventional national accounts. Modified accounting aggregates can thus be used in defining and measuring environmentally sound and sustainable economic growth. Further development objectives need to be assessed by more comprehensive, though necessarily less integrative, systems of environment statistics and indicators. Integrative frameworks for the different statistical systems in the fields of economy, environment and population would facilitate the provision of comparable data for the analysis of integrated development.     

Nitrogen recovery in an integrated system for wastewater treatment and tilapia production

An integrated system, consisting of Up-flow Anaerobic Sludge Blanket (UASB)-duckweed-tilapia ponds was used for recovery of sewage nutrients and water recycling. A UASB reactor with 40 liter working volume was used as pre-treatment unit followed by a series of three duckweed ponds for nitrogen recovery. The treated effluent and duckweed biomass was used to feed fishponds stocked with Nile tilapia (Oreochromis niloticus). The UASB reactor was fed with raw, domestic sewage at 6 h hydraulic retention time. The three duckweed ponds were stocked with Lemna gibba and fed with UASB effluent at 15 days hydraulic retention time. Nitrogen recovery from UASB effluent via duckweed biomass represented 81% of total nitrogen removal and 46.5% from the total nitrogen input to the system. In subsequent fishponds the nitrogen recovery from duckweed as fish feed was in the range of 13.4–20%. This nitrogen in fish biomass represented 10.6–11.5 g N from the total nitrogen in the raw sewage fed to the UASB reactor. The growth performance of tilapia (Oreochromis niloticus) showed specific growth rates (SGR) in the range of 0.53–0.97. The range of feed conversion ratio (FCR) and protein efficiency ratio (PER) were 1.2–2.2 and 2.1–2.28, respectively. The results of the experiments showed total fish yield and net fish yield in the range of 17–22.8 ton/ha/y and 11.8–15.7 ton/ha/y respectively. In conclusion UASB-duckweed-tilapia ponds provide marketable by-products in the form of duckweed and fish protein, which represent a cost recovery for sewage treatment.     

The disposal of a lime water treatment residue on soil and spoil material from a coalmine: a glasshouse investigation

Eragrostis tef (Zucc.), Cenchrus ciliaris L., and Digitaria eriantha Steud. were grown in a soil (Psammentic Haplustalf) and spoil material from a coalmine both treated with a lime water treatment residue (WTR) at rates of 0, 50, 100, 200, and 400 g kg(-1). The yield of the grasses, from the sum of the three harvests, and concentrations of B, Ca, Cu, K, Fe, Mg, Mn, N, Na, P, and Zn in foliage from the second harvest were determined. The yield of grasses grown in the soil decreased exponentially as WTR application increased. The yields of C. ciliaris, D. eriantha, and E. tef (in the 400 g kg(-1) WTR treated soil) decreased by 74.4, 78.7, and 59.8%, respectively, when compared with the control treatments. In the spoil, the yield of E. tef and D. eriantha decreased by 13.6% and and D. eriantha by 23.9%, while an increase was observed for C. ciliaris (45.4%), at the highest WTR application rate. No relationship existed between yield of E. tef and WTR application rate when grown in the spoil, while a weak negative linear relationship (p < 0.05) was found for D. eriantha and a positive linear relationship existed for C. ciliaris. Magnesium concentrations of the grasses were positively correlated to WTR application rate. Grasses grown in the soil had higher Na concentrations, while those grown in the spoil typically had higher B, N, and Zn concentrations. The decreases in yield were attributed to nutrient deficiencies (notably Zn), induced by high WTR application rates that led to high substrate pH. Disposal of high rates of WTR on the mine materials was not recommended.     

ECOLOGICAL MODELS AND ENVIRONMENTAL STUDIES1

The recent world-wide trend towards centralization of all environmental management functions into one regulatory agency has illustrated the necessity for resources management agencies to adopt a total systems viewpoint. Environmental systems are typically complex and multi-dimensional in nature. Mathematical models for the management of air, water and land resources have found wide acceptance among planners and decision-makers. Ecological models of life processes have not reached the same state of development or acceptance. A general review of ecological systems theory and examples of the types of ecological models that have been developed to date arc presented in this paper. With this material as a background and given the vast literature on engineering and economic models, a conceptual framework for an approach to environmental studies and the analysis of polluted environmental systems is presented.     

A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg(II) in environment

A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of λphage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ≤ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples.     

"Reference Biospheres" for solid radioactive waste disposal: the BIOMASS methodology

The BIOMASS Theme 1 project has developed a methodology for the logical and defensible construction of 'assessment biospheres': mathematical representations of biospheres used in the total system performance assessment of radioactive waste disposal. The BIOMASS Methodology provides a systematic approach to decision making, including decisions on how to address biosphere change. The BIOMASS Methodology was developed through consultation and collaboration with many relevant organisations, including regulators, operators and a variety of independent experts. It has been developed to be practical and to be consistent with recommendations from ICRP and IAEA on radiation protection in the context of the disposal of long-lived solid radioactive wastes. The five main steps in the methodology are described in this paper. The importance of a clear assessment context, to clarify intentions and to support a coherent biosphere assessment process within an overall repository performance assessment, is strongly emphasised. A well described assessment context is an important tool for ensuring consistency across the performance assessment as a whole. The use of interaction matrices has been found to be helpful in clarifying the interactions between different habitats within the biosphere system and the significant radionuclide transfer pathways within the biosphere system. Matrices also provide a useful means of checking for consistency.     

Decolorization of Remazol Black-B using a thermotolerant yeast, Kluyveromyces marxianus IMB3

The ability of Kluyveromyces marxianus IMB3 to decolorize Remazol Black-B dye was investigated. The effect of environmental conditions, such as pH and temperature were examined. No noticeable effects on decolorization were observed when pH varied from 3.0-5.5. Maximum colour removal, 98%, was achieved at 37 degrees C. Little or no colour removal was detected when K. marxianus IMB3 was incubated under anaerobic conditions. Further investigation, in which decolorization was monitored under extreme temperatures and low pH (to inhibit growth) and using ten fold dense inoculum, revealed that decolorization was due to biosorption to the yeast cells and not due to a metabolic reaction.     

Aerial measurement of radioxenon concentration off the west coast of Vancouver Island following the Fukushima reactor accident

In response to the Fukushima nuclear reactor accident, on March 20th, 2011, Natural Resources Canada conducted aerial radiation surveys over water just off the west coast of Vancouver Island. Dose-rate levels were found to be consistent with background radiation, however a clear signal due to ¹³³Xe was observed. Methods to extract ¹³³Xe count rates from the measured spectra, and to determine the corresponding ¹³³Xe activity concentration, were developed. The measurements indicate that ¹³³Xe concentrations on average lie in the range of 30-70 Bq/m³.