Ultra-structural observations on the lymphoid organs of the freshwater catfish, Clarias batrachus (Linnaeus)

Light microscopic and ultra-structural studies of the lymphoid tissues such as blood immunocytes, spleen and pronephros of the freshwater catfish, Clarias batrachus, were carried out. The peripheral blood showed nucleated erythrocytes, total leucocytic count (TLC) more than that observed in mammalian blood and leucocytes with morphological appearance similar to the mammalian white blood cells (WBCs). The spleen and pronephros showed presence of numerous lymphocytes, monocytes and nucleated red blood cells (RBCs) along with hemosiderin-containing macrophages. The morphology of lymphoid organs of the catfish has been discussed in light of the evolution of the immune system in this class of vertebrates.     

Fungicidal activity and molecular modeling of fusarubin analogues from Fusarium oxysporum

Abstract

Fusarubin analogues of Fusarium oxysporum f. sp. ciceris were investigated for antifungal activity in vitro against five soil borne phytopathogenic fungi. 3-O-Methyl-8-O-methyl-fusarubin was inhibitory towards S. sclerotiorum (EC₅₀ 0.33?mmol L^?1) and Sclerotium rolfsii (EC₅₀ 0.38?mmol L^?1). A structure–antifungal activity relationship of fusarubin analogues was established from their activity performance. Possible mechanism of action of these compounds was studied using molecular docking and simulations against three target enzymes which revealed receptor ligand binding affinity. Docking of 3-O-methyl-8-O-methyl-fusarubin into the succinate dehydrogenase site revealed formation of salt bridge, hydrogen bond, π–anion, π–alkyl, and Van der Waals interactions.     

Changing diversity of hymenopteran parasitoids from organically and conventionally managed tea-ecosystem of North Bengal, India

Tea, Camellia sinensis (L.) O. Kuntze, is grown conventionally (application of chemical fertilizer and pesticides) as well as organically in Darjeeling hills and adjoining plains. Studies on parasitic Hymenoptera gain relevance due to their effective role as biocontrol agents for maintenance of ecological balance and as biological indicators of health of tea agro-ecosystem. Diversity of the hymenopterans was studied using Shannon-Weaver index (1963) both at morphospecies and family levels. The analysis reflected the changing diversity of this group with season, pesticide application and the condition of the tea crop. It further indicated that mainly four parasitoid groups such as braconids, ichneumonids, eulophids and scelionids, showed greater diversity in organic plantation as compared to conventional. Since species (= RTU: recognizable taxonomic unit) diversity and family diversity indices agreed with one another a surrogacy based system (family for RTU) could be adopted for diversity analysis.     

Oxidant stress, antioxidants and nitric oxide in traffic police of Hyderabad, India

Exposure to environmental pollutants is known to be harmful to health, in general, and to lungs in particular. In this respect, traffic police are at particular risk due to the nature of their job, since they are exposed to emissions from the vehicles. Here, we show that in the traffic police of Hyderabad city, India, the plasma levels of lipid peroxides are high, whereas the concentrations of the nitric oxide are low. In addition, the levels of various antioxidants in the RBC lysate such as catalase, superoxide dismutase and glutathione peroxidase were found to be low with no significant alteration in plasma ceruloplasmin levels. These results suggest that exposure to air pollutants, a major portion of which is due to emissions from the vehicles, can increase oxidant stress, decrease the levels of antioxidants and nitric oxide. This imbalance in the oxidant/antioxidant system may lead to lung damage and is likely to cause respiratory problems in individuals exposed to air pollution.     

Organochlorine pesticide residues in catfish,Tachysurus thalassinus (Ruppell, 1835), from the South Patches of the Bay of Bengal

Twenty-one wild brown rats (Rattus norvegicus) were collected from four areas of Milan (Italy). Main organs were harvested from each rat, fixed in 10% neutral buffer formalin, and embedded in paraffin. Organ sections were stained with hematoxylin and eosin, kidney sections were also stained with the Ziehl-Neelsen method. Graphite furnace atomic absorption spectrophotometry was performed on all kidney samples. The presence of inclusion bodies and karyocytomegaly was scored. Six adult rats out of 21 (28.6%) showed kariocytomegaly and intranuclear inclusion bodies of the renal proximal tubules, characteristic of lead toxicity. The nuclear inclusion bodies were always Ziehl-Neelsen positive. The six rats which showed the characteristic histological alterations ascribed to lead, had lead concentrations statistically higher than the three control rats (4 microg/g wet wt. versus 0.09 microg/g wet wt., P<0.001) and than the captured wild rats. Intranuclear inclusion bodies were found in rats with kidney lead concentrations greater than 0.6 microg/g wet weight. There were significant differences between the four areas of capture. The incidence of intranuclear inclusion bodies and karyocytomegaly in proximal tubular cells was higher in rats with high lead levels detected by spectrophotometry. The present results indicate a renal lead concentration of 0.6 microg/g wet weight as a threshold to detect histologic kidney changes due to lead absorption. Results demonstrate that the examination of adult free-living rats may be considered an appropriate method to obtain a rapid, sensitive, easy, cost effective estimate of lead contamination in urban areas. Animal models allow us to evaluate potential human health risk associated with environmental contamination.     

Phytoplanktonic Community of Organically Polluted Tropical Reservoirs in Eastern India

Plankton is a kaleidoscopic spectrum of organisms with repre-sentatives from almost all phyla of animals and thousands of non-flowering plants. Unicellular protozoans, invertebrates and bacteriato diatoms all drift around as the plankton community. The produc-tion rate of plankton is determined by a host of environmental pa-rameters like physico-chemical properties of water and soil, mete-orological characteristics of the region, and morphometric and hy-drographic features of the water body[3]…     

Effect of different design aspects of pipe for earth air tunnel heat exchanger system: A state of art

Earth air tunnel heat exchanger (EATHE) is a promising passive technique to provide thermal comfort condition in buildings. EATHE system uses undisturbed temperature of the ground for heating/cooling of air. Despite the several advantages, this technique has not become much popular owing to its high capital cost (mainly pipe cost and trench excavation cost) and large land area requirements. The primary objective of this study is to present a comprehensive review of different EATHE pipe layouts, pipe properties and positioning of the pipe with their advantages and limitations. It is observed that the ring pipe layout is the most cost-effective pipe-layout for small size EATHE system because it saves excavation cost by using a trench of the existing foundation of the building. However, Grid pipe-layout is an ideal layout for a large size EATHE system. Multi-layer pipe layouts should be used to reduce the land area requirement significantly. Moreover, EATHE system can be installed beneath the building (under building foot-print) to eliminate extra land area requirement for the installation of EATHE system. This review article shows that the Initial capital cost and land area requirement for the EATHE system can be substantially reduced by using appropriate pipe layout. It can be concluded that if EATHE system is installed with proper design strategies, it will be a clean and cost-effective method for building heating/cooling with significant power savings.