A knowledge network to promote the use and valorization of wild food plants in the Pantanal and Cerrado,Brazil

Regional Environmental Change - The conservation and use of wild food plants should include local people in the decision-making process, and many countries have done so. However, interacting with...     

Blood markers among residents from a coal mining area

Environmental Science and Pollution Research - Coal extraction and processing generate environmental contamination, which has several negative impacts on human health. Hematological and biochemical...     

Ecotoxicity of imidacloprid to soil invertebrates in two tropical soils with contrasting texture

Environmental Science and Pollution Research - Imidacloprid is one of the most commercialized insecticides in agriculture in the world, with a broad spectrum of action. However, little is known...     

Protective effect of gamma-oryzanol against manganese-induced toxicity in Drosophila melanogaster

Environmental Science and Pollution Research - Manganese (Mn) is an essential element that, in excess, seems to be involved in the development of different neurodegenerative conditions....     

One-year Surveillance of Human Enteric Viruses in Raw and Treated Wastewaters,Downstream River Waters,and Drinking Waters

Human enteric viruses are a major cause of waterborne diseases, and can be transmitted by contaminated water of all kinds, including drinking and recreational water. The objectives of the present study were to assess the occurrence of enteric viruses (enterovirus, norovirus, adenovirus, hepatitis A and E virus) in raw and treated wastewaters, in rivers receiving wastewater discharges, and in drinking waters. Wastewater treatment plants’ (WWTP) pathogen removal efficiencies by adenovirus quantitative real-time PCR and the presence of infectious enterovirus, by cell culture assays, in treated wastewaters and in surface waters were also evaluated. A total of 90 water samples were collected: raw and treated wastewaters (treated effluents and ultrafiltered water reused for industrial purposes), water from two rivers receiving treated discharges, and drinking water. Nested PCR assays were used for the identification of viral DNA/RNA, followed by direct amplicon sequencing. All raw sewage samples (21/21), 61.9 % of treated wastewater samples (13/21), and 25 % of ultrafiltered water samples (3/12) were contaminated with at least one viral family. Multiple virus families and genera were frequently detected. Mean positive PCRs per sample decreased significantly from raw to treated sewage and to ultrafiltered waters. Moreover, quantitative adenovirus data showed a reduction in excess of 99 % in viral genome copies following wastewater treatment. In surface waters, 78.6 % (22/28) of samples tested positive for one or more viruses by molecular methods, but enterovirus-specific infectivity assays did not reveal infectious particles in these samples. All drinking water samples tested negative for all viruses, demonstrating the effectiveness of treatment in removing viral pathogens from drinking water. Integrated strategies to manage water from all sources are crucial to ensure water quality.     

Carbon paste electrodes modified with biosolids, soils and biocomposites utilized to study the interaction between organic matter and copper

Carbon paste electrodes (CPEs) modified with a biosolid, two types of soils with different amounts of organic matter (OM), and two biocomposites (soils mixed with a biosolid) were used to assess and compare the Cu(II) ion retention properties of the organic matter contained in the samples. The accumulation of Cu(II) on the surface of the modified carbon paste electrodes (MCPEs) was performed under open-circuit conditions. When comparing the response of the MCPEs while assessing parameters such as pH, preconcentration time, and adsorption/desorption capacity, it was found that the reaction mechanism of the two soils is different between the soils and dissimilar from the biosolid; while the biocomposites show reaction mechanisms that are intermediate between those of the soils and the biosolid. This was proven with the use of infrared spectroscopy, since the FTIR spectra show similarities between the two soils and significant differences between the soils and the biosolid.     

Influence of Sampling Effort on the Estimated Richness of Road-Killed Vertebrate Wildlife

Road-killed mammals, birds, and reptiles were collected weekly from highways in southern Brazil in 2002 and 2005. The objective was to assess variation in estimates of road-kill impacts on species richness produced by different sampling efforts, and to provide information to aid in the experimental design of future sampling. Richness observed in weekly samples was compared with sampling for different periods. In each period, the list of road-killed species was evaluated based on estimates the community structure derived from weekly samplings, and by the presence of the ten species most subject to road mortality, and also of threatened species. Weekly samples were sufficient only for reptiles and mammals, considered separately. Richness estimated from the biweekly samples was equal to that found in the weekly samples, and gave satisfactory results for sampling the most abundant and threatened species. The ten most affected species showed constant road-mortality rates, independent of sampling interval, and also maintained their dominance structure. Birds required greater sampling effort. When the composition of road-killed species varies seasonally, it is necessary to take biweekly samples for a minimum of one year. Weekly or more-frequent sampling for periods longer than two years is necessary to provide a reliable estimate of total species richness.     

Voltammetric analysis of metallothioneins and copper (II) in fish for water biomonitoring studies

An major research area in environmental chemistry is the development of methods for the analysis of biomarkers. Metallothioneins are used as biomarkers in studies of heavy metals exposure in water, because metallothioneins are synthesized and accumulated when organisms are exposed to toxic concentrations of pollutants. In this work, simple and sensitive voltammetric methods were developed for metallothionein and copper (II) determinations in fish liver Lepomis gibbosus. Both analytical methodologies were optimized and applied to samples extracted from individuals previously submitted to sub-lethal toxicological trials with copper sulphate (CuSO₄) and cadmium chloride (CdCl₂). The obtained results showed that both methods are very precise, sensitive, and involve simple sample preparation processes. Moreover, metallothioneins showed better correlation with the toxic exposure than Cu²⁺. To the best of our knowledge, this is the first time that hepatic metallothioneins and Cu²⁺ contents are voltammetrically determined in order to be compared in their function as heavy metal biological indicators.