Effect of ultrasonication and Fenton oxidation on biodegradation of bis(2-ethylhexyl) phthalate (DEHP) in wastewater sludge

The presence of bis(2-ethylhexyl) phthalate (DEHP) and its metabolites, i.e. 2-ethylhexanol, 2-ethylhexanal, and 2-ethylhexanoic acid in wastewater sludge (WWS) were investigated during aerobic digestion and Bacillus thuringiensis (Bt)-based fermentation of WWS. Ultrasonication and Fenton oxidation pre-treatment was applied to improve biodegradability of WWS and bioavailability of the target compounds for digestion and fermentation. DEHP and 2-ethylhexanoic acid were observed at higher concentration, meanwhile 2-ethylhexanol and 2-ethylhexanal were observed at lower concentration in WWS. After 20-day aerobic digestion, DEHP removal was 72%, 89%, and 85%, and 2-ethylhexanoic acid removal was 71%, 84%, 79%, respectively for raw, ultrasonicated, and Fenton-oxidized sludges. Bt was found to degrade DEHP, leading to DEHP removal of 21%, 40%, and 30%, respectively for raw, ultrasonicated, and Fenton-oxidized sludges in the fermentation. The results suggested that aerobic stabilization and Bt-based fermentation can remove the phthalates, and pre-treatment of WWS was also effective in improvement of DEHP biodegradation. Hence, Bt-based biopesticide production from WWS can be applied safely when taking into consideration the phthalate contaminants.     

Activated carbon/Fe(3)O(4) nanoparticle composite: fabrication, methyl orange removal and regeneration by hydrogen peroxide

In the water treatment field, activated carbons (ACs) have wide applications in adsorptions. However, the applications are limited by difficulties encountered in separation and regeneration processes. Here, activated carbon/Fe₃O₄ nanoparticle composites, which combine the adsorption features of powdered activated carbon (PAC) with the magnetic and excellent catalytic properties of Fe₃O₄ nanoparticles, were fabricated by a modified impregnation method using HNO₃ as the carbon modifying agent. The obtained composites were characterized by X-ray diffraction, scanning and transmission electron microscopy, nitrogen adsorption isotherms and vibrating sample magnetometer. Their performance for methyl orange (MO) removal by adsorption was evaluated. The regeneration of the composite and PAC-HNO₃ (powdered activated carbon modified by HNO₃) adsorbed MO by hydrogen peroxide was investigated. The composites had a high specific surface area and porosity and a superparamagnetic property that shows they can be manipulated by an external magnetic field. Adsorption experiments showed that the MO sorption process on the composites followed pseudo-second order kinetic model and the adsorption isotherm date could be simulated with both the Freundlich and Langmuir models. The regeneration indicated that the presence of the Fe₃O₄ nanoparticles is important for a achieving high regeneration efficiency by hydrogen peroxide.     

Occurrence, fate and antibiotic resistance of fluoroquinolone antibacterials in hospital wastewaters in Hanoi, Vietnam

Occurrence and behavior of fluoroquinolone antibacterial agents (FQs) were investigated in hospital wastewaters in Hanoi, Vietnam. Hospital wastewater in Hanoi is usually not treated and this untreated wastewater is directly discharged into one of the wastewater channels of the city and eventually reaches the ambient aquatic environment. The concentrations of the FQs, ciprofloxacin (CIP) and norfloxacin (NOR) in six hospital wastewaters ranged from 1.1 to 44 and from 0.9 to 17 micrgl(-1), respectively. Total FQ loads to the city sewage system varied from 0.3 to 14 g d(-1). Additionally, the mass flows of CIP and NOR were investigated in the aqueous compartment in a small wastewater treatment facility of one hospital. The results showed that the FQ removal from the wastewater stream was between 80 and 85%, probably due to sorption on sewage sludge. Simultaneously, the numbers of Escherichia coli (E. coli) were measured and their resistance against CIP and NOR was evaluated by determining the minimum inhibitory concentration. Biological treatment lead to a 100-fold reduction in the number of E. coli but still more than a thousand E. coli colonies per 100ml of wastewater effluent reached the receiving water. The highest resistance was found in E. coli strains of raw wastewater and the lowest in isolates of treated wastewater effluent. Thus, wastewater treatment is an efficient barrier to decrease the residual FQ levels and the number of resistant bacteria entering ambient waters. Due to the lack of municipal wastewater treatment plants, the onsite treatment of hospital wastewater before discharging into municipal sewers should be considered as a viable option and consequently implemented.     

Modeling of metal binding in tropical Fluvisols and Acrisols treated with biosolids and wastewater

There is growing concern about the accumulation of metals in tropical agricultural soils. In this study, experimental results from batch studies were used to test whether multi-surface geochemical models could describe metal binding in selected Vietnamese soils. The multi-surface models considered metal binding to iron hydroxides (using the diffuse layer model), organic matter (Stockholm Humic model and NICA-Donnan model), and phyllosilicate clay (Gaines-Thomas equation) as well as complexation to dissolved organic and inorganic ligands in the solution phase. We found that for total dissolved Cd, Cu and Zn the two multi-surface models being tested provided very good model fits for all soils, as evidenced by low root-mean square errors between model predictions and observations. These results suggest that organic matter is an important sorbent for many metals in these soils. However, poor fits were obtained for Cr(III), Mn and Pb for all soils. The study also suggests that the pH is the main factor that controls the solubility of metals in tropical Fluvisols and Acrisols subjected to application of biosolids and wastewater, and that advanced multi-surface models can be used to simulate the binding and release of many trace metals.     

Human ecological effects of tropical storms in the coastal area of Ky Anh (Ha Tinh,Vietnam)

Vietnam is prone to tropical storms. Climate change effects contribute to sea level rise, floods, progression of the low water line and coastal erosion. This paper inventories the perception of local people, assesses and values main aspects of the livelihood damage caused by the tropical storms of the period 2008–2013 in three coastal communes of the Ky Anh District of the Ha Tinh Province in Central Vietnam. The communes were selected because the location of their coastal line is perpendicular to the storm itself, which made them prone to damage. The effects of increasingly extreme weather conditions on three communities in an area most affected by storms and floods on the local residents and their responses to these changing environmental conditions are analyzed and assessed. The results of questionnaires completed by randomly selected local inhabitants of these communes show that storms and related hazards such as flood, sea level rise and heavy rain are perceived as the most impacting climate change intensified phenomena on agriculture and aquaculture, livestock, household property and income. Opinions and measured data provided by the commune and district authorities allow estimating the total direct cost of the tropical storm at 1.56 million $US (The used conversion rate VND/$US is 21,730 when the research was conducted in 2014) during the period 2008–2013. The long-term costs of adaptation and social impact measures will be significantly higher. Details of the monetary figures allow identifying the physical and natural capital of the area as being most affected by the storm. Trend and cost analysis show that the total financial support for hazard prevention and management during 2014–2019 is estimated at 1.19 up to 1.32 million $US. Local stakeholders indicate that climate change adaptation should not be limited to technical measures such as strengthening dikes, but also should target planting protection forests and mangroves and land use planning. Financial support for the relocation policy, stakeholder involvement and integrating climate change adaptation in both the socioeconomic development master plan and local land use planning are also of importance.     

Impact of metal stress on the production of secondary metabolites in <Emphasis Type="Italic">Pteris vittata</Emphasis> L. and associated rhizosphere bacterial communities

Plants adapt to metal stress by modifying their metabolism including the production of secondary metabolites in plant tissues. Such changes may impact the diversity and functions of plant associated microbial communities. Our study aimed to evaluate the influence of metals on the secondary metabolism of plants and the indirect impact on rhizosphere bacterial communities. We then compared the secondary metabolites of the hyperaccumulator Pteris vittata L. collected from a contaminated mining site to a non-contaminated site in Vietnam and identified the discriminant metabolites. Our data showed a significant increase in chlorogenic acid derivatives and A-type procyanidin in plant roots at the contaminated site. We hypothesized that the intensive production of these compounds could be part of the antioxidant defense mechanism in response to metals. In parallel, the structure and diversity of bulk soil and rhizosphere communities was studied using high-throughput sequencing. The results showed strong differences in bacterial composition, characterized by the dominance of Proteobacteria and Nitrospira in the contaminated bulk soil, and the enrichment of some potential human pathogens, i.e., Acinetobacter, Mycobacterium, and Cupriavidus in P. vittata’s rhizosphere at the mining site. Overall, metal pollution modified the production of P. vittata secondary metabolites and altered the diversity and structure of bacterial communities. Further investigations are needed to understand whether the plant recruits specific bacteria to adapt to metal stress.     

Exposure assessment of lead to workers and children in the battery recycling craft village,Dong Mai,Vietnam

Human exposure to lead (Pb) due to uncontrolled Pb-acid battery recycling has been an environmental health issue in newly developed industrial regions. We conducted a human monitoring survey in Dong Mai, a battery recycling village in Vietnam, to assess exposure status to Pb. Lead level was measured in hair, blood and urine samples of residents in Dong Mai and two reference sites during 4 years spanning 2007–2011. In Dong Mai, Pb levels in three matrixes were significantly higher than those in reference sites. Blood Pb levels of all adults and children exceeded 10 μg/dL, the Centers for Disease Control and Prevention definition of an elevated blood Pb level. Clear increase of urinary δ-aminolevulinic acid (ALA) level with increasing blood Pb level indicated disruption of heme synthesis. One adult exceeded 100 μg/dL of blood Pb, where encephalopathy is of concern. The blood Pb levels achieved various toxic effect threshold values, and elevated blood Pb was not limited to recycling workers, but was also in children and women of reproductive age. Serious pollution status of Dong Mai village suggests an importance of further monitoring surveys in various developing Asian countries.     

Soil contamination by brominated flame retardants in open waste dumping sites in Asian developing countries

In Asian developing countries, large amounts of municipal wastes are dumped into open dumping sites each day without adequate management. This practice may cause several adverse environmental consequences and increase health risks to local communities. These dumping sites are contaminated with many chemicals including brominated flame retardants (BFRs) such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs).BFRs may be released into the environment through production processes and through the disposal of plastics and electronic wastes that contain them.The purpose of this study was to elucidate the status of BFR pollution in municipal waste dumping sites in Asian developing countries. Soil samples were collected from six open waste dumping sites and five reference sites in Cambodia, India, Indonesia, Malaysia, and Vietnam from 1999 to 2007. The results suggest that PBDEs are the dominant contaminants in the dumping sites in Asian developing countries, whereas HBCD contamination remains low. Concentrations of PBDEs and HBCDs ranged from ND to 180 μg/kg dry wt and ND to 1.4 μg/kg dry wt, respectively, in the reference sites and from 0.20 to 430 μg/kg dry wt and ND to 2.5 μg/kg dry wt, respectively, in the dumping sites. Contamination levels of PBDEs in Asian municipal dumping sites were comparable with those reported from electronic waste dismantling areas in Pearl River delta, China.     

Persistence of white spot syndrome virus in shrimp ponds and surrounding areas after an outbreak

White Spot Syndrome Virus (WSSV) is the major and most serious pathogen in shrimp aquaculture industries. By using a sensitive PCR-based detection technique followed by sequencing multiple PCR products for confirmation, we address to the question of whether WSSV can persist in shrimp ponds and surrounding areas after an outbreak. The seawater samples were taken from two shrimp ponds and surrounding canals in a coastal area in northern Vietnam, Quang Ninh Province where the shrimps cultured in the two ponds had been killed due to a WSSV outbreak in April 2001 and the ponds were thereafter abandoned. A total of 480 seawater samples (30 samples each for July and December of 2001 and 2002) were subjected to WSSV genome detection. Although the detection rates of WSSV genome were generally gradually declined in seawater environments of both diseased shrimp ponds and surrounding canals, WSSV was still detected with rates of more than 10% in the diseased ponds and lower in surrounding canals in December 2002, 20 months after the WSSV outbreak.     

Contamination by perfluorinated compounds in water near waste recycling and disposal sites in Vietnam

There are very few reports on the contamination by perfluorinated chemicals (PFCs) in the environment of developing countries, especially regarding their emission from waste recycling and disposal sites. This is the first study on the occurrence of a wide range of PFCs (17 compounds) in ambient water in Vietnam, including samples collected from a municipal dumping site (MD), an e-waste recycling site (ER), a battery recycling site (BR) and a rural control site. The highest PFC concentration was found in a leachate sample from MD (360 ng/L). The PFC concentrations in ER and BR (mean, 57 and 16 ng/L, respectively) were also significantly higher than those detected in the rural control site (mean, 9.4 ng/L), suggesting that municipal solid waste and waste electrical and electronic equipment are potential contamination sources of PFCs in Vietnam. In general, the most abundant PFCs were perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluoroundecanoic acid (PFUDA; <1.4–100, <1.2–100, and <0.5–20 ng/L, respectively). Interestingly, there were specific PFC profiles: perfluoroheptanoic acid and perfluorohexanoic acid (88 and 77 ng/L, respectively) were almost as abundant as PFOA in MD leachate (100 ng/L), whereas PFNA was prevalent in ER and BR (mean, 17 and 6.2 ng/L, respectively) and PFUDA was the most abundant in municipal wastewater (mean, 5.6 ng/L), indicating differences in PFC contents in different waste materials.