Transabdominal chorionic villus sampling in the second and third trimesters of pregnancy: Chromosome quality,reporting time,and feto-maternal bleeding

Transabdominal chorionic villus sampling (TA-CVS) was performed in 210 pregnancies from 13 to 38 weeks using a double-needle technique. The sampling success was comparable to first-trimester TA-CVS and the diagnostic success rate was 98.2 per cent for the short-term technique and 99.3 per cent for cultured villi. Two fetuses could not be karyotyped. We found the chromosome quality to be similar to that in the first trimester, comparing the number of G-bands and other chromosome attributes. There were no unintended losses in a group (n = 142) with no sonographic abnormality, except for one death in utero at 38 weeks, 20 weeks after sampling. Chromosomal aberrations were seen in 19 per cent of cases with abnormal sonograms (n = 58). One case of a discordant karyotype was found (false-negative prediction of Down's syndrome by the short-term preparation). There were no cases of fetal demise due to feto-maternal bleeding. It is suggested that double-needle TA-CVS in advanced pregnancies combines the advantages of rapid karyotyping of chromosomes of good quality and low risk for the fetus, and seems to be easier to practise and is probably safer than cordocentesis.     

博茨瓦纳欧科范果湿地1972～2000年泛滥方式

欧科范果三角洲地区泛滥范围年年在变化.这种变化与安哥拉高地集水区的区域性降水及当地降雨量有关.我们采用了1972～2000年的3000多幅卫星图像来描绘湿地的格局,其中从1985～2000年的图像几乎是NOAAAVHRR每日连续传送的,1972年以来的其它图像是从Landsat传感器上传下来的,其连续性次之.对AVHRR图像每10天为一期,用无监督分类方法分成陆地和水体.对LandsatTM和ERS2-ATSR数据进行分析的结果,与测算的淹没区域89%相吻合.结果显示欧科范果湿地近期30年期间的变化约在2450～11400km2之间.     

Understanding determinants of home range behaviour of feral cats as introduced apex predators in insular ecosystems: a spatial approach

The introduced feral cat (Felis catus) is a widespread generalist with flexible social behaviour and an apex predator without major interspecific competitors in insular ecosystems that evolved in the absence of predators. Mechanistic definitions consider an animal’s home range to be the spatial expression of a cognitive map that is kept up-to-date with the status of critical resources that contribute to animal fitness. We assumed there are two major determinants structuring the home range of cats as apex predators in insular ecosystems: the distribution of critical food resources and conspecific distribution. We hypothesized that cats structure their home ranges by optimizing the use of staple critical food resources and that as a consequence of the presence of rich resources cats tend to socialise, aggregate and share space. We carried out spatial analyses using location data for feral cats tracked using lightweight GPS collars in conjunction with the suitability value of rabbit patches and their associated ownership costs for cats within a New Zealand braided-river environment. Cat home ranges and spatial distribution, especially for females, were related to the inclusion of rabbit patches within home ranges with higher mean value than the average of neighbourhood patches in the landscape. Cats showed solitary behaviour but tolerance to conspecific presence by sharing high-use areas and high-value rabbit patches, mostly at different times, resulting in occasional encounters among males and females. Home range size and patterns of spatial overlap were dependant on sex and season. Solitary spacing patterns as consequence of innate preferences together with resource constraints may regulate feral cat population densities.     

Characterization of products formed in the reaction of ozone with alpha-pinene: case for organic peroxides

The generation of reactive oxygen species (ROS) and their subsequent induced pulmonary and systemic oxidative stress has been implicated as an important molecular mechanism of PM-mediated toxicity. However, recent work has shown that there is significant ROS associated with ambient PM. In order to understand the formation mechanisms as well as understand the potential health effects of particle-bound oxidative species, the alpha-pinene-O(3) oxidation chemical system was studied to elucidate the structures of reaction products using liquid chromatography-multiple stage mass spectrometry (LC-MS(n)). The classes of compounds identified based on their multiple stage-MS fragmentation patterns, mechanistic considerations of alpha-pinene-O(3) oxidation, and general fragmentation rules, of the products from this reaction system were highly oxygenated species, predominantly containing hydroperoxide and peroxide functional groups. The oxidant species observed were clearly stable for the 1-3 h that elapsed during aerosol collection and analysis, and probably for much longer, thus rendering it possible for these species to bind onto particles forming fine particulate organic peroxides that concentrate on the particles and could deliver concentrated doses of ROS in vivo to tissue.     

Spatial contaminant heterogeneity: quantification with scale of measurement at contrasting sites

Material within the terrestrial environment is rarely homogeneously distributed, either spatially or temporally. One consequence of heterogeneity is that uncertainty is usually generated in measurements that are taken with the aim of characterising the environment. For example, a measurement of analyte concentration within soil taken from one sampling location on contaminated land can vary substantially when compared against another sample taken at effectively the same nominal location. The measurement uncertainty arising from the heterogeneity can substantially limit the reliability of the interpretations made upon environmental investigations. The sampling uncertainty usually outweighs the analytical uncertainty from the laboratory, often by a factor of 20 or more. One approach to reducing the uncertainty is to design a more suitable sampling strategy. This might be achieved by predicting the degree of heterogeneity prior to the investigation, but this is often difficult to achieve accurately. Another approach, which was investigated here, is to actually characterise the heterogeneity prior to the main investigation using rapid and inexpensive technology, such as in situ measurement techniques. In situ portable X-ray fluorescence (PXRF) and X-ray microprobe (XMP) techniques were employed to test the feasibility of this approach. Two contrasting contaminated land sites were chosen to characterise the two-dimensional spatial heterogeneity of heavy metal contamination in topsoil at a range of scales (50 m to 0.001 m). The spatial heterogeneity of contaminants, expressed as relative standard deviations, was found to differ between the two sites by a factor of two, largely due to the mode of deposition of pollution. The study also indicated that the heterogeneity did not change systematically with the scale of measurement between sampling locations at either site.     

Designing the Masoala National Park in Madagascar Based on Biological and Socioeconomic Data

Abstract: Conservation biologists have developed powerful tools for reserve selection and design over the past two decades, yet seldom are protected areas actually designed on scientific grounds. Using fundamental biological and socioeconomic principles of conservation science, we designed a new protected area and its multiple-use zone on the Masoala Peninsula in the humid forest zone of Madagascar. The explicit design criteria determined the data gathered for the work, which included (1) spatial distribution and quality of habitat, (2) the areas and species at greatest risk, (3) the relationship between environmental gradients and species distributions, (4) current and predicted human settlement and land and resource use, and (5) the economic potential of natural forest management as an alternative to deforestation. We used a geographic information system to integrate these data layers and applied the design criteria to develop a park proposal that balanced human and wildlife needs. The proposal won the approval of local residents, and a national decree in 1997 designated 2100 km² of rainforest and three satellite marine reserves as the Masoala National Park, with a surrounding multiple-use zone of approximately 1000 km². The new park is Madagascar's largest protected area and protects more lowland (<400 m) humid forest habitat than the entire reserve system combined, a significant step forward in conserving a globally important ecoregion. Consideration of local needs and the national economy was a key element in gaining approval for the Masoala Park. Such an approach toward reserve design could be applied elsewhere to improve chances of establishing and maintaining protected areas over the long term.     

Effects of Amazonian flying rivers on frog biodiversity and populations in the Atlantic rainforest

Given the speed at which humans are changing the climate, species with high degrees of endemism may not have time to avoid extinction through adaptation. We investigated through teleconnection analysis the origin of rainfall that determines the phylogenetic diversity of rainforest frogs and the effects of microclimate differences in shaping the morphological traits of isolated populations (which contribute to greater phylogenetic diversity and speciation). We also investigated through teleconnection analysis how deforestation in Amazonia can affect ecosystem services that are fundamental to maintaining the climate of the Atlantic rainforest biodiversity hotspot. Seasonal winds known as flying rivers carry water vapor from Amazonia to the Atlantic Forest, and the breaking of this ecosystem service could lead Atlantic Forest species to population decline and extinction in the short term. Our results suggest that the selection of morphological traits that shape Atlantic Forest frog diversity and their population dynamics are influenced by the Amazonian flying rivers. Our results also suggest that the increases of temperature anomalies in the Atlantic Ocean due to global warming and in the Amazon forest due to deforestation are already breaking this cycle and threaten the biodiversity of the Atlantic Forest hotspot.