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351.
The metabolism of 14C-labelled lactate was investigated in the horseshoe crab Limulus polyphemus and the stone crab Menippe mercenaria. When a bolus of (14C-U)-D-lactate was injected into L. polyphemus, there was substantial release pf 14CO2 into the medium. In the case of M. mercenaria, 14CO2 release was also observed after injection of (14C-U)-L-lactate into experimental individuals. Analysis of the distribution of radioactivity in whole body extracts of both species revealed easily detectable amounts of radioactivity in the glycogen fraction, although the bulk of the radioactivity was in the cation, anion/neutral and CO2 fractions. To investigate the metabolism of lactate further, 14C-labelled lactate was injected into large individuals of L. polyphemus and M. mercenaria, and the distribution of radioactivity was determined in the hemolymph, muscle and hepatopancreas. Utilization of (14C-U)-D-lactate by L. polyphemus resulted in the accumulation of significant amounts of labelled glucose in all three body compartments as well as the production of labelled glycogen in the telson levator muscle and hepatopancreas. Utilization of (14C-U)-L-lactate resulted in a similar pattern of glucose and glycogen labelling in the hemolymph, cheliped muscle and hepatopancreas of M. mercenaria. These studies demonstrate that both L. polyphemus and M. mercenaria have the capacity for glyco- and gluconeogenesis using lactate as the substrate. 相似文献
352.
Ingo?NarberhausEmail author Claudine?Theuring Thomas?Hartmann Susanne?Dobler 《Chemoecology》2004,14(1):17-23
Summary. Several species of Longitarsus take up, metabolize and
store pyrrolizidine alkaloids (PAs) from their host plants. In feeding experiments using
radioactively labeled PAs of different types we examined the time course of the sequestration
process in L. jacobaeae and
L. aeruginosus. We found that adapted species efficiently
store PAs for at least two weeks without major losses. During that time, there is virtually
no change in the ratio of tertiary alkaloids to stored non-toxic
N-oxides, regardless of chemical form fed to the beetles. This
implies a transient N-oxidation process where the alkaloids are
only temporarily accessible to the enzyme. A dissection experiment with
L. aeruginosus six days after uptake of labeled PAs
showed that the tertiary alkaloids are not found in the hemolymph but are stored in the
elytra and other body compartments. This conforms with earlier experiments that localized the
enzymes site of action in the hemolymph. Furthermore we show that different total alkaloid
doses in the diet of L. jacobaeae and the potentially less
adapted L. succineus do not affect the ratio of recovered
N-oxides to tertiary molecules. Thus, the efficiency of the
N-oxidizing enzyme is not dependent on the concentration of
alkaloids offered. 相似文献
353.
354.
Sabine?HeimEmail author Jan?Schwarzbauer Ralf?Littke 《Environmental Chemistry Letters》2004,2(1):21-25
Specific anthropogenic substances derived from seepage and leakage water from a waste deposit landfill were analysed in groundwater and drainage effluents using gas chromatography-mass spectrometry (GC/MS). Here, we demonstrate the application of an organic marker approach to study lateral and vertical distribution of contamination as well as the long-term emission behaviour. Source-specific and environmentally-stable pesticides, plasticisers and pharmaceuticals, such as N,N-diethyl toluamide, N-butyl benzene sulfonamide, clofibric acid, mecoprop and bisphenol A, were quantitatively analysed. A distinct lateral expansion was detected. Although temporal variations were high, no significant trend was observed. 相似文献
355.
356.
Nathália Nascimento Thales A. P. West Lisa Biber-Freudenberger Eráclito R. de Sousa-Neto Jean Ometto Jan Börner 《Journal of Land Use Science》2020,15(2-3):127-141
ABSTRACT Deforestation driven by agricultural expansion is a major threat to the biodiversity of the Amazon Basin. Modelling how deforestation responds to environmental policy implementation has thus become a policy relevant scientific undertaking. However, empirical parameterization of land-use/cover change (LUCC) models is challenging due to the high complexity and uncertainty of land-use decisions. Bayesian Network (BN) modelling provides an effective framework to integrate various data sources including expert knowledge. In this study, we integrate remote sensing products with data from farm-household surveys and a decision game to model LUCC at the BR-163, in Brazil. Our ‘business as usual’ scenario indicates cumulative forest cover loss in the study region of 8,000 km2 between 2014 and 2030, whereas ‘intensified law-enforcement’ would reduce cumulative deforestation to 1,600 km2 over the same time interval. Our findings underline the importance of conservation law enforcement in modulating the impact of agricultural market incentives on land cover change. 相似文献
357.
Alfonso Marzal Maribel Reviriego Florentino de Lope Anders Pape Møller 《Behavioral ecology and sociobiology》2007,61(10):1573-1580
Immune responses constitute a major way for hosts to defend themselves against parasites. Because hosts do not habitually
produce strong responses all the time, immune responses might be costly to produce or maintain. We tested experimentally if
the production of a response to a challenge with a novel antigen resulted in a cost in terms of fitness using the highly colonial
house martin Delichon urbica as a model system. We injected adult breeding birds during laying of the first clutch with either Newcastle disease virus
(NDV) or a control injection, and the clutch was subsequently removed to induce relaying. NDV stimulates the non-specific
immune system, causing production of antibodies during a period of more than 2 weeks. Accordingly, we found an increase in
leukocyte counts in experimental birds compared to controls. Experimental treatment reduced the frequency of re-laying, caused
a delay in timing of relaying and a reduction in brood size. Quality of nestlings in terms of body size, body mass and T-cell-mediated
immune response did not differ significantly between treatments. Overall, seasonal reproductive success differed significantly
between treatments, showing that the production of an immune response by adult birds is costly in terms of future fecundity. 相似文献
358.
Mathias?RickingEmail author Helga?Neumann-Hensel Jan?Schwarzbauer Anders?Svenson 《Environmental Chemistry Letters》2004,2(3):109-112
The proportions of elemental sulphur species in sediment from a contaminated area and a reference sediment sample were compared with the acute toxicity measured in short-term toxicity tests. Octameric elemental sulphur was separated into three operationally defined fractions. Here we show that octameric elemental sulphur contributes to the acute toxicity measured by bioluminescence inhibition. Our findings show that only a small part of extractable elemental sulphur was effective during exposure to the test organisms. A firmly bound fraction of sulphur was found in a contaminated sediment. This information has to be taken into account while interpreting the acute sediment toxicity. 相似文献
359.
In mammals with solitary females, the potential for males to monopolize matings is relatively low, and scramble competition polygyny is presumed to be the predominant mating system. However, combinations of male traits and mating tactics within this type of polygyny have been described. The main aim of our study was to identify the relative importance of, and interactions among, potential determinants of contrasting male reproductive tactics, and to determine their consequences for male reproductive success in a small solitary nocturnal Malagasy primate, the gray mouse lemur (Microcebus murinus). We studied their mating behavior over three consecutive annual mating seasons. In addition, we determined the genetic relationships among more than 300 study animals to quantify the reproductive success of individual males. We found that, with a given relatively low overall monopolization potential, successful male mouse lemurs roamed extensively in search of mates, had superior finding ability and mated as early as possible. However, contest competition was important too, as temporary monopolization was also possible. Males exhibited different mating tactics, and heavier males had a higher reproductive success, although most litters had mixed paternities. Switching between tactics depended on short-term local variation in monopolization potential determined by a pronounced dynamic in fertilization probability, number of alternative mating opportunities, and the operational sex ratio. This study also revealed that the dynamics of these determinants, as well as the mutual interactions between them, necessitate a detailed knowledge of the mating behavior of a species to infer the impact of determinants of alternative mating tactics.Electronic Supplementary Material Supplementary material is available in the online version of this article at
Communicated by S. AlbertsThis revised version was published online in August 2004 with corrections to Figure 2. 相似文献
360.
Julia?FischerEmail author Dawn?M.?Kitchen Robert?M.?Seyfarth Dorothy?L.?Cheney 《Behavioral ecology and sociobiology》2004,56(2):140-148
Free-ranging adult male baboons give loud two-syllable wahoo calls during dawn choruses, interactions between groups, when chasing females, and in aggressive interactions with other males. Previous research has shown that the rate and duration of these contest wahoos are correlated with a males competitive ability: high-ranking males call more often, call at faster rates, and call for longer bouts than do low-ranking males. Here we report that acoustic features of wahoos also reveal information about male competitive ability. High-ranking males give wahoos with higher fundamental frequencies (F0) and longer hoo syllables. Within-subject analyses revealed that, as males fall in rank, the hoo syllables tend to shorten within a period of months. As males age and continue to fall in rank, F0 declines, hoo syllables shorten, and formant dispersion decreases. Independent of age and rank, within bouts of calling F0 declines and hoo syllables become shorter. Because wahoos are often given while males are running or leaping through trees, variation in these acoustic features may function as an indicator of a males stamina. The acoustic features of contest wahoos thus potentially allow listeners to assess a males competitive ability.Communicated by C. Nunn 相似文献