Single drop microextraction and gas chromatography–mass spectrometry for the determination of diflufenican, mepanipyrim, fipronil, and pretilachlor in water samples

An analytical method for the determination of diflufenican, mepanipyrim, pretilachlor, and fipronil in water samples was developed using single drop microextraction in the direct immersion mode and gas chromatography–mass spectrometry. A factorial fractionated design of type 2^6–1 at two levels was performed, to study the influence of experimental variables such as ionic strength, pH, agitation speed, extraction time, drop volume, and sample volume. To establish the optimal conditions for the variables that were significant, a Doehlert design was performed. The optimum conditions of extraction were 1 μL of heptane immersed in 4.0 mL of sample with continuous agitation at 500 rpm for 30 min at room temperature. The developed method proved to have good linearity for the range studied. The detection limits were 0.07 μg L^?1 for diflufenican, 0.03 μg L^?1 for mepanipyrim, 0.08 μg L^?1 for pretilachlor, and 1.39 μg L^?1 for fipronil. The method was validated on river water samples, showing the absence of matrix effect and recoveries ranged from 90.1 to 107.8 %. The results show that the method developed is accurate, sensitive, rapid, simple, and low cost, so it is recommended for application in the analysis of these different classes of pesticides in water samples.     

Cadmium and lead in bovine milk in the mining area of the Caudal River (Spain)

The levels of cadmium and lead in 36 raw bovine milk samples were analysed. These samples come from seven farms with a semi-extensive grazing system and were collected between the autumn of 2007 and the winter of 2008. All the farms were located in Asturias (Spain), a zone of great industrial and mining activity in the proximities of the Caudal River. The samples were collected in sterile precleaned polypropylene tubes and frozen until the analysis. After a lyophilization process, the samples were treated with nitric acid and microwave treatment. Cadmium and lead determinations were carried out using inductively coupled plasma mass spectrometry with sensibility under 0.2 ppb for liquid matrix. The lead content was found to vary from 0.71 to 16.06 μg/kg wet weight (w.w.), and the cadmium was lower than 2 μg/kg w.w. The levels of lead in milk are higher in those farms near zones of storage of mining waste depots, thermal power and areas with high levels of traffic. All the values found are in concordance with research carried out at non-polluted areas, and those for the lead are well below the European Union limitations.     

The valuation of historical sites: a case study of Valdivia,Chile

The economic valuation of cultural heritage is an area of increasing interest and an important research topic in the emerging field of cultural economics. Many services and values associated with cultural heritage are not traded in markets, and their estimation requires methods developed for the valuation of non-market goods, such as those used in environmental economics. This paper applies the contingent valuation method with double dichotomous choice to estimate the value of historical sites in the city of Valdivia, Chile. The valuation exercise was implemented by designing a hypothetical guided walking tour to a cluster of historical sites in the city centre, and surveying tourists visiting the city during the summer of 2004. Parametric and non-parametric statistical methods were used to estimate the survival distribution and the mean and median estimates of the willingness-to-pay (WTP). The study emphasizes the importance of explicitly treating heterogeneous preferences and the sensitivity of the survival distribution to the estimation methods used.     

The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil

The production and properties of a rhamnolipid-type biosurfactant, synthesized by the Pseudomonas aeruginosa LBM10 strain, isolated from a southern coastal zone in Brazil, were investigated. The assays were conducted in a rotary shaker at 30 degrees C and 180 rpm for a period of 96 h. Soybean oil and sodium nitrate were the best sources of carbon and nitrogen, respectively. A nitrogen-limiting condition (C/N ratio of 100) was favorable to biosurfactant production. The formation of stable emulsions was better in saline concentrations below 0.5%, pH values in the range from 6 to 9 and temperatures in the range from 35 to 40 degrees C, maintaining about 80% of its original activity for salinity up to 3% and 120 min of exposure at 100 degrees C. The biosurfactant may be produced with this microorganism using renewable substrates that are readily available, reaching values of 1.42 g l(-1) measured as rhamnose. This biosurfactant has interesting and useful properties for many industrial applications.     

Persistence of gemfibrozil, naproxen and mefenamic acid in natural waters

The occurrence of pharmaceuticals in natural waters is a potential threat to human nutrition and ecosystem quality. The persistence of the acidic pharmaceuticals gemfibrozil, naproxen and mefenamic acid was studied in surface waters of Maracaibo Lake and Tule reservoir (Venezuela) under laboratory conditions. A quick and easy analytical method was developed for the determination of the acidic drugs at microgram per liter levels using aqueous derivatization, liquid–liquid extraction and gas chromatography–mass spectrometry. Pharmaceuticals degradation followed a pseudo first-order kinetic and their half-lives were calculated for every experimental condition. Under sunlight, naproxen and mefenamic acid were degraded at moderate rates with half-lives from 9.6 ± 0.5 to 27.0 ± 6.6 days, while gemfibrozil had a higher persistence (t _1/2 = 119.5 ± 15.6 − 288.8 ± 61.3 days).     

Prediction of hourly O3 concentrations using support vector regression algorithms

In this paper we present an application of the Support Vector Regression algorithm (SVMr) to the prediction of hourly ozone values in Madrid urban area. In order to improve the training capacity of SVMrs, we have used a recently proposed approach, based on reductions of the SVMr hyper-parameters search space. Using the modified SVMr, we study different influences which may modify the ozone prediction, such as previous ozone measurements in a given station, measurements in neighbors stations, and the influence of meteorologic variables. We use statistical tests to verify the significance of incorporating different variables into the SVMr. A comparison with the results obtained using a neural network (multi-layer perceptron) is also carried out. This study has been carried out in 5 different stations of the air pollution monitoring network of Madrid, so the conclusions raised are backed by real data. The final result of the work is a robust and powerful software for tropospheric ozone prediction in Madrid. Also, the prediction tool based on SVMr is flexible enough to incorporate any other prediction variable, such as city models, or traffic patters, which may improve the prediction obtained with the SVMr.     

Impact of error self-perception of aerobic capacity in the safety and efficacy of the lifeguards

Introduction. The strong physical demands that are required of lifeguards during rescues also require an accurate self-perception of one's fitness level to be able to regulate the intensity of effort. Objectives. The aim of this study was to determine the real aerobic capacity (RAC) and to compare it with two self-reported measurements: subjective appraisal of aerobic capacity (SAAC) and appraisal of physical exercise (APE). Methods. Fifty-two professional lifeguards were included in the study. For an objective assessment of RAC, the lifeguards’ maximum oxygen uptake (V_O2max) values were measured during treadmill stress tests. A fitness assessment questionnaire was used to obtain the SAAC and APE values. Results. We found a statistically significant association between the APE and RAC variables in the contingency analysis (p?V_O2max value below 43 ml kg^?1?min^?1 considered their aerobic capacity to be high or very high. Conclusion. This self-perception error of true aerobic capacity could lead to premature fatigue during a rescue, endangering both the lifeguard's life and the life of the victim. These data may help lifeguards and beach managers to become aware of the need to know lifeguards’ true physical conditions through testing and structured training programs.     

Recent strandings of the giant jellyfish Rhizostoma luteum Quoy and Gaimard, 1827 (Cnidaria: Scyphozoa: Rhizostomeae) on the Atlantic and Mediterranean coasts

We present reports of sightings of living and stranded specimens of Rhizostoma luteum on the Atlantic coast of Morocco and along the south shore of the Iberian Peninsula in June–July 2012 and in January–February 2013. During summer 2012 and following the dominant currents, the jellyfish first appeared in the Gulf of Cadiz west of the Strait of Gibraltar. Subsequently, seven additional sightings were reported east of the Strait, in the Alboran Sea. In winter 2013, another event of stranded individuals of this species occurred in the Gulf of Cadiz. A phylogenetic analysis performed on the mitochondrial cytochrome c oxidase I (COI) gene sequence in specimens from both stranding events confirmed the morphological classification, ratifying that R. luteum differs from Rhizostoma octopus and Rhizostoma pulmo. This study records the presence of this species for the first time in 60 years.     

A relational demography model of workgroup identification: testing the effects of race,race dissimilarity,racial identification,and communication behavior

This study tested a relational demography model of workgroup identification. We theorized that early in workgroup formation, (a) racial identification would moderate the influence of racial dissimilarity on member communication behavior (frequency and length of verbal participation) during a group task, (b) member communication behaviors would mediate the effect of racial dissimilarity on workgroup identification, and that (c) these effects would be affected non‐symmetrically by racial group membership (White, Black). This model was tested among a sample of 211 undergraduates (113 White and 98 Black), working in 43 temporary workgroups. Whites' racial identification moderated the relationship between racial dissimilarity and member communication behaviors, which in turn partially mediated the effect of racial dissimilarity on workgroup identification, generally consistent with our model. Among Black workgroup members support for our model was limited. Copyright © 2008 John Wiley & Sons, Ltd.